BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0260.0002.p (504 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0102 [S] COG4716 Myosin-crossreactive antigen Sau Staphylococc... 564 e-160 SPy0470 [S] COG4716 Myosin-crossreactive antigen Spy Streptococc... 539 e-153 L180241 [S] COG4716 Myosin-crossreactive antigen Lla Lactococcus... 528 e-149 lin0483 Non COG 275 2e-73 >SA0102 [S] COG4716 Myosin-crossreactive antigen Sau Staphylococcus aureus N315 Length = 591 Score = 564 bits (1453), Expect = e-160, Method: Compositional matrix adjust. Identities = 275/509 (54%), Positives = 356/509 (69%), Gaps = 7/509 (1%) Query: 1 MEDHFECLWDLFRSIPSLELEGASVLDEFYWLNKDDPNYTLRRATVKQGQDAHTDGLFTL 60 ME+HFECLWDLFRSIPSLE++ ASVLDEFYWLNK+DPNY+ R KQGQ TDG FTL Sbjct: 83 MENHFECLWDLFRSIPSLEIDNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTL 142 Query: 61 SEQAQKELIALFLATRKQMETKRIDEVFGRDFFNSNFWLYWRTMFAFEEWHSALEMKLYL 120 ++ A KE++ L L + ++ +I +VF DFFNSNFW+YW+TMFAFE WHSA+EM+ YL Sbjct: 143 TKTAIKEILDLCLTNEEDLDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYL 202 Query: 121 HRFIHHIGGLPDFSTLKFTKYNQYESLVLPLSKWLLAQGVNFRYGVGVTDIDFDIKRGRK 180 RF+HHIGGL DFS LKFTKYNQYESLVLP+ ++L + GV F Y V V DI D+ +K Sbjct: 203 MRFVHHIGGLADFSALKFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQK 262 Query: 181 QATAIRWLENGIEGSLIIGADDLVFMTIGSLTENSDMGDHHTAARLNEGPAPAWDLWRRI 240 A I +G S+ + DDLVF+T GS+TE+S GD+ T A + +W LW+ + Sbjct: 263 IAREILIHRHGKAESIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNL 322 Query: 241 AAKDAAFGRPNVFCGNIPASKW-ESATITTLDKRIPAYIRKIAKRDPFSGKVVTGGIVTV 299 A + FG P+ FC NIP W SAT TT +K I I I KRDP +GK VTGGI+T+ Sbjct: 323 ARQSPEFGNPDKFCQNIPKKSWFVSATSTTNNKEIIDTIESICKRDPLAGKTVTGGIITI 382 Query: 300 KDSSWLLSWTTNRQPHFKRQPSDQVVVWVYSLFVDKAGDYVRKPMQDCTGEEITQEWLYH 359 DS+W +S+T NRQ FK QP +++ W+Y+L+ D GDY++KP+ +C+G EI QEWLYH Sbjct: 383 NDSAWQMSFTINRQQQFKDQPENEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYH 442 Query: 360 LGVPVASIPELAAGAAKTVPVMMPYVTSFFMPRQAGDRPDVVPKDAVNFAFIGQFAESGE 419 LGV I +LA + T+PV MPY+TS+FM R GDRP VVP + N +FIG FAE+ E Sbjct: 443 LGVSTDKIEDLAKHVSNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLSFIGNFAET-E 501 Query: 420 RDCIFTTEYSVRTAMEAVYILLKIERGVPEVFNSTYDIRKLLAAMGRLRDGNEI-EIPGP 478 RD +FTTEYSVRTAMEAVY LL I+RG+PEV NST+D+R L+ A+ L D ++ EI Sbjct: 502 RDTVFTTEYSVRTAMEAVYQLLNIDRGIPEVINSTFDLRVLMDAVYELNDHQDLREITKD 561 Query: 479 AFLREL----ILKKIEATELGELLNKYHL 503 + +++L LKKI+ T + LL ++ L Sbjct: 562 SKMQKLALAGFLKKIKGTYIETLLKEHKL 590 >SPy0470 [S] COG4716 Myosin-crossreactive antigen Spy Streptococcus pyogenes M1 GAS Length = 590 Score = 539 bits (1388), Expect = e-153, Method: Compositional matrix adjust. Identities = 261/508 (51%), Positives = 347/508 (68%), Gaps = 6/508 (1%) Query: 1 MEDHFECLWDLFRSIPSLELEGASVLDEFYWLNKDDPNYTLRRATVKQGQDAHTDGLFTL 60 ME+HFEC+WD++RSIPSLE+ GAS LDEFYWL+KDDPN + R K+G DG +TL Sbjct: 83 MENHFECMWDMYRSIPSLEIPGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTL 142 Query: 61 SEQAQKELIALFLATRKQMETKRIDEVFGRDFFNSNFWLYWRTMFAFEEWHSALEMKLYL 120 +Q+ KELI L + T + + + I+E F DFF SNFW+YW TMFAFE+WHSA+EM+ Y Sbjct: 143 GKQS-KELIHLIMKTEESLGDQTIEEFFSEDFFKSNFWVYWATMFAFEKWHSAVEMRRYA 201 Query: 121 HRFIHHIGGLPDFSTLKFTKYNQYESLVLPLSKWLLAQGVNFRYGVGVTDIDFDIKRGRK 180 RFIHHI GLPDF++LKF KYNQY+S+V P+ +L + V+ ++ VTDI + G+K Sbjct: 202 MRFIHHIDGLPDFTSLKFNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKK 261 Query: 181 QATAIRWLENGIEGSLIIGADDLVFMTIGSLTENSDMGDHHTAARLNEGPAPAWDLWRRI 240 A I +G ++ + DDLVF+T GS+TE+S G HH A+ + +W+LW + Sbjct: 262 VAKTIHMTVSGEAKAIELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENL 321 Query: 241 AAKDAAFGRPNVFCGNIPASKW-ESATITTLDKRIPAYIRKIAKRDPFSGKVVTGGIVTV 299 AA+ FG P VF ++PA W SAT T I YI ++ RD GKV TGGI+T+ Sbjct: 322 AAQSDDFGHPKVFYQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITI 381 Query: 300 KDSSWLLSWTTNRQPHFKRQPSDQVVVWVYSLFVDKAGDYVRKPMQDCTGEEITQEWLYH 359 DS+W++S+ +RQPHFK Q ++ VW+Y L+ + G+YV K +++CTG+EIT+EWLYH Sbjct: 382 TDSNWMMSFAIHRQPHFKEQKENETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYH 441 Query: 360 LGVPVASIPELAA-GAAKTVPVMMPYVTSFFMPRQAGDRPDVVPKDAVNFAFIGQFAESG 418 LGVPV I +LA+ TVPV MPY+TS+FMPR GDRP V+P +VN AFIG FAES Sbjct: 442 LGVPVDKIKDLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESP 501 Query: 419 ERDCIFTTEYSVRTAMEAVYILLKIERGVPEVFNSTYDIRKLLAAMGRLRDGNEI---EI 475 RD +FTTEYS+RTAMEAVY L +ERG+PEVFNS YDIR+LL A L D I ++ Sbjct: 502 SRDTVFTTEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDL 561 Query: 476 PGPAFLRELILKKIEATELGELLNKYHL 503 P PA + ++ KKI+ T + ELL +L Sbjct: 562 PIPALIEKIGHKKIKDTFIEELLKDANL 589 >L180241 [S] COG4716 Myosin-crossreactive antigen Lla Lactococcus lactis Length = 587 Score = 528 bits (1360), Expect = e-149, Method: Compositional matrix adjust. Identities = 261/503 (51%), Positives = 348/503 (69%), Gaps = 9/503 (1%) Query: 1 MEDHFECLWDLFRSIPSLELEGASVLDEFYWLNKDDPNYTLRRATVKQGQDAHTDGLFTL 60 ME+HFECLWDLFR+IPSLE++ AS+LDEFY +++DDPN + R +G+ DG FTL Sbjct: 83 MENHFECLWDLFRTIPSLEVDDASILDEFYRIDRDDPNSSNCRIIHNRGERVDDDGKFTL 142 Query: 61 SEQAQKELIALFLATRKQMETKRIDEVFGRDFFNSNFWLYWRTMFAFEEWHSALEMKLYL 120 S +AQ+E+I LF+ + + K I++ F +FF SNFWLYW +MFAFE+WHSA+EM+ Y+ Sbjct: 143 SPKAQEEIIKLFMTKEELLVGKTIEDCFTDEFFKSNFWLYWCSMFAFEKWHSAIEMRRYI 202 Query: 121 HRFIHHIGGLPDFSTLKFTKYNQYESLVLPLSKWLLAQGVNFRYGVGVTDIDFDIKRGRK 180 RFIHHI GLPDFS LKFTKYNQYESLV PL +L GV+F+YG V +++ D G+K Sbjct: 203 MRFIHHIEGLPDFSALKFTKYNQYESLVKPLISYLKDHGVDFKYGAQVENVEVDFSNGKK 262 Query: 181 QATAIRWLENGIEGSLIIGADDLVFMTIGSLTENSDMGDHHTAARLNEGPAPAWDLWRRI 240 A AI + + I+ + +DLVF+T GS+TE+S G AA +W LW+ + Sbjct: 263 VAKAIVFADKEIKE---LTENDLVFVTNGSITESSTQGSPTQAAPKTSELGGSWKLWQNL 319 Query: 241 AAKDAAFGRPNVFCGNIPASKW-ESATITTLDKRIPAYIRKIAKRDPFSGKVVTGGIVTV 299 A + FG P+VFC IP W SAT+T + +I Y K+ R SGKV+TGGI+TV Sbjct: 320 AKQSEEFGHPDVFCKEIPKEAWVVSATVTWKNLKIQPYFEKLTHRQLRSGKVITGGIITV 379 Query: 300 KDSSWLLSWTTNRQPHFKRQPSDQVVVWVYSLFVDKAGDYVRKPMQDCTGEEITQEWLYH 359 KDS+WLLS+TT+RQPHFK Q + V WVY L + G+Y++KP++DCTGEEITQE LYH Sbjct: 380 KDSNWLLSFTTHRQPHFKEQNDQETVTWVYGLLSNTPGNYIKKPIEDCTGEEITQELLYH 439 Query: 360 LGVPVASIPELAAGAAKTVPVMMPYVTSFFMPRQAGDRPDVVPKDAVNFAFIGQFAESGE 419 LG+P I T+PV MP++TS+FM R+ GDRP VVP+ +VN AF+G FAE+ E Sbjct: 440 LGMPEEEIEAFVKENTNTIPVYMPFITSYFMLREPGDRPLVVPEQSVNLAFLGNFAET-E 498 Query: 420 RDCIFTTEYSVRTAMEAVYILLKIERGVPEVFNSTYDIRKLLAAMGRLRDGNEIEIPGPA 479 RD +FTTEYSVRTAMEAVY LL ++RGVPEV+ S YD+R L ++ L D ++E + Sbjct: 499 RDTVFTTEYSVRTAMEAVYQLLNVDRGVPEVYASAYDLRTLASSAYYLSDKQKLEDLELS 558 Query: 480 FLR----ELILKKIEATELGELL 498 F++ ++ LKKI+ T + ELL Sbjct: 559 FIKKQALKVGLKKIKGTYIEELL 581 >lin0483 Non COG Length = 566 Score = 275 bits (704), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 167/465 (35%), Positives = 243/465 (52%), Gaps = 18/465 (3%) Query: 2 EDHFECLWDLFRSIPSLELEGASVLDEFYWLNKDDPNYTLRRATVKQGQ--DAHTDGLFT 59 E+ +E WDLF SIPSL++ SV +E + P + R K DAH+ G Sbjct: 111 EETYENFWDLFSSIPSLDMPNFSVTEEILNFDHLHPTHAQARLVDKDRNILDAHSMGF-- 168 Query: 60 LSEQAQKELIALFLAT-RKQMETKRIDEVFGRDFFNSNFWLYWRTMFAFEEWHSALEMKL 118 + L+ LAT ++++ I + F FF +NFW W+T FAF++W S E + Sbjct: 169 --NNNDRMLMTKLLATPEEKLDNLTIRDWFDEHFFETNFWYMWQTTFAFQKWSSLFEFRR 226 Query: 119 YLHRFIHHIGGLPDFSTLKFTKYNQYESLVLPLSKWLLAQGVNFRYGVGVTDIDFDIKRG 178 Y++R + + + T NQYESL+LPL +L V+F V DIDF G Sbjct: 227 YMNRMMLEFSRIDTLEGVTRTPLNQYESLILPLKTFLDKHHVDFTINQTVEDIDFKDAPG 286 Query: 179 RKQATAIRWLENGIEGSLIIGADDLVFMTIGSLTENSDMGDHHTAARLNEGPAPAWDLWR 238 ATA+ L +G ++ +G DD V MT +T+++ +GD +T A E + +LW Sbjct: 287 -ITATALH-LSDG--STIELGPDDDVIMTNACMTDSATLGDMNTPAPKPEEKPISGELWY 342 Query: 239 RIAAKDAAFGRPNVFCGNIPASKWESATITTLDKRIPAYIRKIAKRDPFSGKVVTGGIVT 298 ++A K G P F G+ + W+S T+T ++ I + P SG ++T Sbjct: 343 KVAQKKPNLGNPEPFFGHEEETNWQSFTVTCHGDKLLKRIERFTGNIPGSG-----ALMT 397 Query: 299 VKDSSWLLSWTTNRQPHFKRQPSDQVVVWVYSLFVDKAGDYVRKPMQDCTGEEITQEWLY 358 KDS+WL+S QPHFK Q ++ + W Y L+ D+ GD+V+KPM++CTGEEI E + Sbjct: 398 FKDSNWLMSTVVAAQPHFKAQDANTTIFWGYGLYPDRVGDFVKKPMKECTGEEILYELMC 457 Query: 359 HLGVPVASIPELAAGAAKTVPVMMPYVTSFFMPRQAGDRPDVVPKDAVNFAFIGQFAESG 418 HL E+ A +P MPY+ + F PR DRP VVP+ + NFA I QF E Sbjct: 458 HLNWQ-DDFEEIKADIINVIPCYMPYIDAQFEPRAMSDRPAVVPEGSTNFAMISQFVEI- 515 Query: 419 ERDCIFTTEYSVRTAMEAVYILLKIERGVPEVFNSTYDIRKLLAA 463 +D +FT EYSVR A AVY LL I++ + V D + L A Sbjct: 516 PKDMVFTEEYSVRAARIAVYTLLDIDKKICPVTPHNRDPKVLAKA 560