BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0259.0004.p (366 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1786 [C] COG1740 Ni,Fe-hydrogenase I small subunit Sty Salmon... 527 e-149 hyaA [C] COG1740 Ni,Fe-hydrogenase I small subunit Eco Escherich... 509 e-144 ZhyaA [C] COG1740 Ni,Fe-hydrogenase I small subunit EcZ Escheric... 507 e-143 ECs1128 [C] COG1740 Ni,Fe-hydrogenase I small subunit Ecs Escher... 507 e-143 STM1539 [C] COG1740 Ni,Fe-hydrogenase I small subunit Sty Salmon... 499 e-141 >STM1786 [C] COG1740 Ni,Fe-hydrogenase I small subunit Sty Salmonella typhimurium LT2 Length = 372 Score = 527 bits (1357), Expect = e-149, Method: Compositional matrix adjust. Identities = 242/361 (67%), Positives = 289/361 (80%), Gaps = 1/361 (0%) Query: 6 ETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHGLECTC 65 ETFY +RR+G+TRRSF KFCSL A+S GLG +IA ALE KPR+PV+W+HGLECTC Sbjct: 5 ETFYQAMRRKGVTRRSFLKFCSLAATSLGLGAGMTPKIAWALENKPRIPVVWIHGLECTC 64 Query: 66 CSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLAVEGNP 125 C+ESFIRS+HPL KDV+LS+ISLDYDD +MAAAG QAE + ++ RY G+Y+LAVEGNP Sbjct: 65 CTESFIRSSHPLAKDVILSLISLDYDDTLMAAAGAQAEEVFDDITTRYAGKYILAVEGNP 124 Query: 126 PLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPIDKVIT 185 PL E GMFCI GG+PF+E+L+ A + A+IAWG CASWGCVQAA+PNPT A PIDKVIT Sbjct: 125 PLGEQGMFCISGGRPFIEKLKKAAAGASAIIAWGNCASWGCVQAARPNPTQATPIDKVIT 184 Query: 186 DKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRSHFDAG 245 DKPIVKVPGCPPI +VM+ ++T++ TF RLPELDR GRP MFY +RIHDKCYRR+HFDAG Sbjct: 185 DKPIVKVPGCPPIPDVMSAIITYMVTFDRLPELDRMGRPLMFYGQRIHDKCYRRAHFDAG 244 Query: 246 QFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSEDGFWD 305 +FVE+WDD+ ARKGYCLYKMGCKGPTTYNACS+ RWN GVSFPIQSGHGC GCSE+GFWD Sbjct: 245 EFVESWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCSENGFWD 304 Query: 306 KGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAV-KRLTHKPDRAVRKDK 364 +GSFY R+ I Q G AD +G+ A G V V HA +A+ +R HK A + + Sbjct: 305 RGSFYSRVVDIPQMGTHSTADTVGLTALGVVAAGVGGHAVASALNQRKRHKQQLAQAEQQ 364 Query: 365 P 365 P Sbjct: 365 P 365 >hyaA [C] COG1740 Ni,Fe-hydrogenase I small subunit Eco Escherichia coli K12 Length = 372 Score = 509 bits (1312), Expect = e-144, Method: Compositional matrix adjust. Identities = 229/329 (69%), Positives = 275/329 (83%) Query: 6 ETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHGLECTC 65 ETFY +RRQG+TRRSF K+CSL A+S GLG A +IA ALE KPR+PV+W+HGLECTC Sbjct: 5 ETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTC 64 Query: 66 CSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLAVEGNP 125 C+ESFIRSAHPL KDV+LS+ISLDYDD +MAAAG QAE + E+ +Y G+Y+LAVEGNP Sbjct: 65 CTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAEEVFEDIITQYNGKYILAVEGNP 124 Query: 126 PLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPIDKVIT 185 PL E GMFCI G+PF+E+L+ A + A+IAWGTCASWGCVQAA+PNPT A PIDKVIT Sbjct: 125 PLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTCASWGCVQAARPNPTQATPIDKVIT 184 Query: 186 DKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRSHFDAG 245 DKPI+KVPGCPPI +VM+ ++T++ TF RLP++DR GRP MFY +RIHDKCYRR+HFDAG Sbjct: 185 DKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAG 244 Query: 246 QFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSEDGFWD 305 +FV++WDD+ ARKGYCLYKMGCKGPTTYNACS+ RWN GVSFPIQSGHGC GC+E+GFWD Sbjct: 245 EFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCAENGFWD 304 Query: 306 KGSFYDRLTTIQQFGIEKNADQIGMAAAG 334 +GSFY R+ I Q G AD +G+ A G Sbjct: 305 RGSFYSRVVDIPQMGTHSTADTVGLTALG 333 >ZhyaA [C] COG1740 Ni,Fe-hydrogenase I small subunit EcZ Escherichia coli O157:H7 EDL933 Length = 372 Score = 507 bits (1305), Expect = e-143, Method: Compositional matrix adjust. Identities = 228/329 (69%), Positives = 274/329 (83%) Query: 6 ETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHGLECTC 65 ETFY +RRQG+TRRSF K+CSL A+S GLG A +IA ALE KPR+PV+W+HGLECTC Sbjct: 5 ETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTC 64 Query: 66 CSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLAVEGNP 125 C+ESFIRSAHPL KDV+LS+ISLDYDD +MAAAG QAE + E+ +Y G+Y+LAVEGNP Sbjct: 65 CTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAEEVFEDIITQYNGKYILAVEGNP 124 Query: 126 PLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPIDKVIT 185 PL E GMFCI G+PF+E+L+ A + A+IAWGTCASWGCVQAA+PNPT A IDKVIT Sbjct: 125 PLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTCASWGCVQAARPNPTQATSIDKVIT 184 Query: 186 DKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRSHFDAG 245 DKPI+KVPGCPPI +VM+ ++T++ TF RLP++DR GRP MFY +RIHDKCYRR+HFDAG Sbjct: 185 DKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAG 244 Query: 246 QFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSEDGFWD 305 +FV++WDD+ ARKGYCLYKMGCKGPTTYNACS+ RWN GVSFPIQSGHGC GC+E+GFWD Sbjct: 245 EFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCAENGFWD 304 Query: 306 KGSFYDRLTTIQQFGIEKNADQIGMAAAG 334 +GSFY R+ I Q G AD +G+ A G Sbjct: 305 RGSFYSRVVDIPQMGTHSTADTVGLTALG 333 >ECs1128 [C] COG1740 Ni,Fe-hydrogenase I small subunit Ecs Escherichia coli O157:H7 Length = 372 Score = 507 bits (1305), Expect = e-143, Method: Compositional matrix adjust. Identities = 228/329 (69%), Positives = 274/329 (83%) Query: 6 ETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHGLECTC 65 ETFY +RRQG+TRRSF K+CSL A+S GLG A +IA ALE KPR+PV+W+HGLECTC Sbjct: 5 ETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAWALENKPRIPVVWIHGLECTC 64 Query: 66 CSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLAVEGNP 125 C+ESFIRSAHPL KDV+LS+ISLDYDD +MAAAG QAE + E+ +Y G+Y+LAVEGNP Sbjct: 65 CTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAEEVFEDIITQYNGKYILAVEGNP 124 Query: 126 PLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPIDKVIT 185 PL E GMFCI G+PF+E+L+ A + A+IAWGTCASWGCVQAA+PNPT A IDKVIT Sbjct: 125 PLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTCASWGCVQAARPNPTQATSIDKVIT 184 Query: 186 DKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRSHFDAG 245 DKPI+KVPGCPPI +VM+ ++T++ TF RLP++DR GRP MFY +RIHDKCYRR+HFDAG Sbjct: 185 DKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAG 244 Query: 246 QFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSEDGFWD 305 +FV++WDD+ ARKGYCLYKMGCKGPTTYNACS+ RWN GVSFPIQSGHGC GC+E+GFWD Sbjct: 245 EFVQSWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCAENGFWD 304 Query: 306 KGSFYDRLTTIQQFGIEKNADQIGMAAAG 334 +GSFY R+ I Q G AD +G+ A G Sbjct: 305 RGSFYSRVVDIPQMGTHSTADTVGLTALG 333 >STM1539 [C] COG1740 Ni,Fe-hydrogenase I small subunit Sty Salmonella typhimurium LT2 Length = 367 Score = 499 bits (1285), Expect = e-141, Method: Compositional matrix adjust. Identities = 243/350 (69%), Positives = 282/350 (80%) Query: 6 ETFYSVIRRQGITRRSFHKFCSLTASSFGLGPLAASRIANALETKPRMPVIWLHGLECTC 65 +TFY V+RR G+TRRSF KFCSLTA+S GL +IA ALE KPR PVIWLHGLECTC Sbjct: 5 DTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGLECTC 64 Query: 66 CSESFIRSAHPLIKDVLLSMISLDYDDMIMAAAGHQAEAILEEARLRYKGRYVLAVEGNP 125 C+ESFIRSAHPL KD +LS+ISLDYDD IMAAAG QAE L + YKG Y++AVEGN Sbjct: 65 CTESFIRSAHPLAKDAILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAVEGNA 124 Query: 126 PLNEGGMFCIDGGKPFVERLRWMAESSMAVIAWGTCASWGCVQAAKPNPTGAVPIDKVIT 185 PLNE GMFCI G+PF+E+L+ ++ + A+IAWG+CASWGCVQAA+PNPT A P+ K+IT Sbjct: 125 PLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSCASWGCVQAARPNPTKATPVHKLIT 184 Query: 186 DKPIVKVPGCPPIAEVMTGLVTFITTFGRLPELDRQGRPKMFYSERIHDKCYRRSHFDAG 245 DKPI+KVPGCPPI EVM+ ++T++ F R+P LDR GRPKMFY +RIHDKCYRR+HFDAG Sbjct: 185 DKPIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAHFDAG 244 Query: 246 QFVEAWDDECARKGYCLYKMGCKGPTTYNACSALRWNGGVSFPIQSGHGCFGCSEDGFWD 305 QFVEAWDDE ARKGYCLYKMGCKGPTTYNACS +RWN GVSFPIQSGHGC GCSEDGFWD Sbjct: 245 QFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSEDGFWD 304 Query: 306 KGSFYDRLTTIQQFGIEKNADQIGMAAAGAVGLTVAAHAAITAVKRLTHK 355 GSFY R T I Q GIE AD+IG+ AG G AHA ++A+K +K Sbjct: 305 YGSFYSRATGIPQTGIEATADKIGLGVAGVAGAAAIAHATVSAIKHARNK 354