BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0257.0009.p (404 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc01435 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate tran... 286 4e-77 AGc3695 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 281 2e-75 mll1448 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate trans... 263 6e-70 BMEI0616 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate tran... 243 5e-64 CC2098 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transf... 243 9e-64 >SMc01435 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Sme Sinorhizobium meliloti Length = 303 Score = 286 bits (733), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 150/293 (51%), Positives = 198/293 (67%), Gaps = 4/293 (1%) Query: 1 VLIAGPTASGKSALALELAQRTGGVVINTDSMQVYRDLRIITARPTAGEEALVPHRLYGH 60 +LI GPTASGKSALA++LAQR GGVVIN DSMQVY L+++TARP E V H LYGH Sbjct: 12 ILITGPTASGKSALAVKLAQRHGGVVINADSMQVYGTLKVLTARPDQSEMGGVEHFLYGH 71 Query: 61 VDAGVNFSAGAWVADAAKVLGEVRAQGRLPIFIGGSGLYFKALTRGLSAVPPIPADVRDG 120 + G +S G W+ +A ++ + +GRLP+F+GG+GLYFKALT GLS +P IP + R Sbjct: 72 IPPGRAYSTGVWLREAEALVARLGKEGRLPVFVGGTGLYFKALTGGLSDMPEIPLETRKR 131 Query: 121 VRARLEQHGVEALHAELAERDPVSAERLKPRDRTRITRALEVVEATGRPLPDWHREGLPP 180 +R RL + G EALH EL+ RDP +A R++P D RI RALE++EATGRP+ + + P Sbjct: 132 LRTRLTEEGAEALHGELSARDPETAGRVRPSDGQRIVRALEIIEATGRPIGSYQQSRGPV 191 Query: 181 LLPPGDFHAVFLAPERETLYARIDARFGSMLDAGALDEVAALAARGLDPLLPAMKAHGVP 240 ++ P + + PER L+ RID RF +ML GA++EV AL A L P +P MKA GVP Sbjct: 192 IIDPARAQKIVVLPERPVLHGRIDRRFETMLARGAVEEVRALLALRLPPEMPVMKAIGVP 251 Query: 241 ALIRHLRGEISREEAAKIGRADTRHYAKRQFTWFRHQLPEFAWV---EPDSAG 290 + L+GE+S ++ + G A TR YAKRQ TWFR+QL E W +PD+ G Sbjct: 252 QIAAMLKGEMSEKQVIEAGAAATRQYAKRQMTWFRNQLDE-TWQRIEDPDALG 303 >AGc3695 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 326 Score = 281 bits (719), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 146/278 (52%), Positives = 187/278 (67%) Query: 1 VLIAGPTASGKSALALELAQRTGGVVINTDSMQVYRDLRIITARPTAGEEALVPHRLYGH 60 +LI GPTASGKSALAL LA+ GVVIN DSMQVY LR++TARP+ E VPHRLYGH Sbjct: 40 ILITGPTASGKSALALRLARERNGVVINADSMQVYDTLRVLTARPSDHEMEGVPHRLYGH 99 Query: 61 VDAGVNFSAGAWVADAAKVLGEVRAQGRLPIFIGGSGLYFKALTRGLSAVPPIPADVRDG 120 V AG +S G W+ D + +L ++R +GR P+ +GG+GLYFKALT GLS +P IP D+R+G Sbjct: 100 VPAGSAYSTGEWLRDISGLLSDLRGEGRFPVIVGGTGLYFKALTGGLSDMPAIPDDLREG 159 Query: 121 VRARLEQHGVEALHAELAERDPVSAERLKPRDRTRITRALEVVEATGRPLPDWHREGLPP 180 +RARL + G LHAEL RDP A+ L+P D RI RALEV+EATG+ + D+ R P Sbjct: 160 LRARLIEEGAAKLHAELVSRDPSMAQMLQPGDGQRIVRALEVLEATGKSIRDFQRASGPM 219 Query: 181 LLPPGDFHAVFLAPERETLYARIDARFGSMLDAGALDEVAALAARGLDPLLPAMKAHGVP 240 ++ P + PER L+ RI+ RF +M+D+GA++EV AL A L P AMKA GV Sbjct: 220 IIDPERAQKFIVLPERPVLHDRINRRFEAMMDSGAVEEVQALLALNLAPDATAMKAIGVA 279 Query: 241 ALIRHLRGEISREEAAKIGRADTRHYAKRQFTWFRHQL 278 + L G + E + A TR YAKRQ TWFR+Q+ Sbjct: 280 QIADMLTGRMGAAEVIEKSAAATRQYAKRQMTWFRNQM 317 >mll1448 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Mlo Mesorhizobium loti Length = 321 Score = 263 bits (671), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 145/287 (50%), Positives = 188/287 (65%), Gaps = 2/287 (0%) Query: 1 VLIAGPTASGKSALALELAQRTGGVVINTDSMQVYRDLRIITARPTAGEEALVPHRLYGH 60 +LIAGPTASGKSALAL+LA+R GGV++N+DSMQ Y L ++TARP A + A VPH LYGH Sbjct: 20 ILIAGPTASGKSALALDLAERAGGVIVNSDSMQGYSVLDVLTARPEAADLARVPHFLYGH 79 Query: 61 VDAGVNFSAGAWVADAAKVLGEVRAQGRLPIFIGGSGLYFKALTRGLSAVPPIPADVRDG 120 V G +S GAW+ D K++ + R P+F+GG+GLYF+AL G+S +P IP +RD Sbjct: 80 VHPGTAYSTGAWLRDVMKLIDD-GMLSRRPVFVGGTGLYFRALAEGISEMPDIPPRIRDR 138 Query: 121 VRARLEQHGVEALHAELAERDPVSAERLKPRDRTRITRALEVVEATGRPLPDWHREGLPP 180 R L++ G LH L D +A +LKP D RI RALEV++A+GR + +W E P Sbjct: 139 WRYELKEQGAVKLHGLLLREDSRAAMQLKPTDSQRIVRALEVLDASGRSILEWQAERGRP 198 Query: 181 LLPPGDFHAVFLAPERETLYARIDARFGSMLDAGALDEVAALAARGLDPLLPAMKAHGVP 240 L+ + + P+R L RI+ RF MLD GAL+EV LAA GLDP LPAMKA GV Sbjct: 199 LIDRQTARFLVIEPDRAALVDRIERRFDQMLDKGALEEVRQLAALGLDPDLPAMKAIGVR 258 Query: 241 ALIRHLRGEISREEAAKIGRADTRHYAKRQFTWFRHQL-PEFAWVEP 286 L + G++S EA + + TR YAKRQ TWFRHQL PE+ + P Sbjct: 259 DLQAAMAGQLSFPEAIERAKIATRQYAKRQATWFRHQLGPEWQRLRP 305 >BMEI0616 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Bme Brucella melitensis Length = 310 Score = 243 bits (621), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 143/280 (51%), Positives = 176/280 (62%) Query: 1 VLIAGPTASGKSALALELAQRTGGVVINTDSMQVYRDLRIITARPTAGEEALVPHRLYGH 60 +LIAGPTASGKSALA+ +A+ TGG ++NTDSMQVY L ++TARP+ A H LYGH Sbjct: 10 ILIAGPTASGKSALAIRMAKATGGFIVNTDSMQVYGVLDLLTARPSRANLAEAEHFLYGH 69 Query: 61 VDAGVNFSAGAWVADAAKVLGEVRAQGRLPIFIGGSGLYFKALTRGLSAVPPIPADVRDG 120 V +S G W D +LG QGR+PIF+GG+GLYF+AL GLS P + A VRD Sbjct: 70 VPPSSTYSTGKWFEDVEALLGRCELQGRVPIFVGGTGLYFRALLGGLSQTPEVSAQVRDH 129 Query: 121 VRARLEQHGVEALHAELAERDPVSAERLKPRDRTRITRALEVVEATGRPLPDWHREGLPP 180 R R+E G +ALHA L RDP A L+P D RI RALEV+E+TG+ L +W + Sbjct: 130 WRGRMEAEGAKALHAVLCVRDPAIAAALQPSDSQRIVRALEVLESTGKSLLEWQKVKGRA 189 Query: 181 LLPPGDFHAVFLAPERETLYARIDARFGSMLDAGALDEVAALAARGLDPLLPAMKAHGVP 240 L+ + L P+R L RI RF +M GA+DEV AL A LDP LPAMKA GV Sbjct: 190 LVDDQSAQKIVLRPDRAWLGVRIARRFSAMWAEGAIDEVRALLALDLDPALPAMKAIGVR 249 Query: 241 ALIRHLRGEISREEAAKIGRADTRHYAKRQFTWFRHQLPE 280 + L +SREEA + TR YAKRQ TWFR+QL E Sbjct: 250 EVSAFLAETMSREEAIERSVIATRQYAKRQSTWFRNQLGE 289 >CC2098 [J] COG0324 tRNA delta(2)-isopentenylpyrophosphate transferase Ccr Caulobacter vibrioides Length = 295 Score = 243 bits (619), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 132/285 (46%), Positives = 173/285 (60%), Gaps = 1/285 (0%) Query: 2 LIAGPTASGKSALALELAQRTGGVVINTDSMQVYRDLRIITARPTAGEEALVPHRLYGHV 61 LIAGPTASGKSA AL+LA+R G ++N DSMQ+Y LR++TA P+ E A PH L+ V Sbjct: 12 LIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEETARAPHHLFQIV 71 Query: 62 DAGVNFSAGAWVADAAKVLGEVRAQGRLPIFIGGSGLYFKALTRGLSAVPPIPADVRDGV 121 D + +S G W+ A + L E++A+G+ I +GG+GLYF+ALT GL+ VPP+P R+ Sbjct: 72 DPALGWSVGRWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADVPPVPETQREIS 131 Query: 122 RARLEQHGVEALHAELAERDPVSAERLKPRDRTRITRALEVVEATGRPLPDWHREGLPPL 181 G L DP + R++ DR R+ RA V TG+ L W + P Sbjct: 132 SLLYAARGETEFREILKPLDPEAEARIESGDRQRLVRAHAVAITTGKSLTAWQTD-TKPA 190 Query: 182 LPPGDFHAVFLAPERETLYARIDARFGSMLDAGALDEVAALAARGLDPLLPAMKAHGVPA 241 L PG + + L P R LYAR DAR M++ GALDEV AL RGLDP LPA+KA G Sbjct: 191 LAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPALPALKAVGYRE 250 Query: 242 LIRHLRGEISREEAAKIGRADTRHYAKRQFTWFRHQLPEFAWVEP 286 HLRGE S ++A R +TR YAKRQ TWFR+Q P++ + P Sbjct: 251 FAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP 295