BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0022.p (294 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20762 [P] COG3627 Uncharacterized enzyme of phosphonate metab... 497 e-140 AGc297 [P] COG3627 Uncharacterized enzyme of phosphonate metabol... 491 e-139 mlr3346 Non COG 478 e-135 phnJ [P] COG3627 Uncharacterized enzyme of phosphonate metabolis... 454 e-127 ECs5081 [P] COG3627 Uncharacterized enzyme of phosphonate metabo... 454 e-127 >SMb20762 [P] COG3627 Uncharacterized enzyme of phosphonate metabolism Sme Sinorhizobium meliloti Length = 297 Score = 497 bits (1279), Expect = e-140, Method: Compositional matrix adjust. Identities = 237/286 (82%), Positives = 249/286 (87%) Query: 5 TYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDV 64 TYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTA+ILGPDDV Sbjct: 6 TYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTASILGPDDV 65 Query: 65 LKVIDQGSDDTTNAISIRKFFGKTAGVATTTSTTDATVIQTRHRIPEASLHAGQVLVYQV 124 LKVIDQG+DDTTNA+SIR FF K A VA TT TT+AT+IQTRHRIPE L GQ LVYQV Sbjct: 66 LKVIDQGADDTTNAVSIRAFFQKVADVAVTTRTTEATIIQTRHRIPEEQLREGQTLVYQV 125 Query: 125 PIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATAYAYPVKVNARYVMDP 184 PIPEPLRFLEPRETETR+MHAL EYGLMHVKLYEDIAR GHIAT YAYPVKV RYVMDP Sbjct: 126 PIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIARNGHIATTYAYPVKVEGRYVMDP 185 Query: 185 SPTPKFDNPKMDNCAALQLFGAGREKRIYAIPPYTTVVSLDFEDHPFTRYRFNAPCALCG 244 SPTPKFDNPKM ALQLFGAGREKRIYA+PPYT VVSLDFEDHPF +F+ PCALCG Sbjct: 186 SPTPKFDNPKMHMSEALQLFGAGREKRIYAVPPYTEVVSLDFEDHPFEVQKFDKPCALCG 245 Query: 245 ADNSYLDEIVTDDKGGRMFVCSDTDFCEQRQAAGHHGTESAAPHKE 290 A+N YLDE+V DDKGGRMFVCSDTD CE R+A GH G A KE Sbjct: 246 AENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHAGAMLAPAAKE 291 >AGc297 [P] COG3627 Uncharacterized enzyme of phosphonate metabolism Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 300 Score = 491 bits (1264), Expect = e-139, Method: Compositional matrix adjust. Identities = 232/276 (84%), Positives = 245/276 (88%) Query: 6 YNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDVL 65 YNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQ+TA+ILGPDDVL Sbjct: 14 YNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQLTASILGPDDVL 73 Query: 66 KVIDQGSDDTTNAISIRKFFGKTAGVATTTSTTDATVIQTRHRIPEASLHAGQVLVYQVP 125 KVIDQG+DDTTNA+SIR FF K A VA TT T DAT+IQTRHRIPE LH+GQVLVYQVP Sbjct: 74 KVIDQGADDTTNAVSIRAFFQKVADVAVTTRTKDATIIQTRHRIPEEELHSGQVLVYQVP 133 Query: 126 IPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATAYAYPVKVNARYVMDPS 185 IPEPLRFLEPRETETR MHAL EYGLMHVKLYEDIAR G I+T YAYPVKV RYVMDPS Sbjct: 134 IPEPLRFLEPRETETRVMHALEEYGLMHVKLYEDIARNGRISTTYAYPVKVAGRYVMDPS 193 Query: 186 PTPKFDNPKMDNCAALQLFGAGREKRIYAIPPYTTVVSLDFEDHPFTRYRFNAPCALCGA 245 PTPKFDNPKM+ ALQLFGAGREKRIYA+PPYT VVSLDFEDHPF RF+ PCALCG Sbjct: 194 PTPKFDNPKMNMSDALQLFGAGREKRIYAVPPYTDVVSLDFEDHPFEIQRFDKPCALCGG 253 Query: 246 DNSYLDEIVTDDKGGRMFVCSDTDFCEQRQAAGHHG 281 +N YLDE+V DDKGGRMFVCSDTD CE R+A GH G Sbjct: 254 ENVYLDEVVLDDKGGRMFVCSDTDHCEDRRAHGHKG 289 >mlr3346 Non COG Length = 299 Score = 478 bits (1230), Expect = e-135, Method: Compositional matrix adjust. Identities = 225/277 (81%), Positives = 241/277 (87%) Query: 5 TYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGPDDV 64 TYNFAYLDEQTKRMIRRAILK IAIPGYQVPFASREMPMPYGWGTGGVQVTA+I+GPDDV Sbjct: 6 TYNFAYLDEQTKRMIRRAILKGIAIPGYQVPFASREMPMPYGWGTGGVQVTASIIGPDDV 65 Query: 65 LKVIDQGSDDTTNAISIRKFFGKTAGVATTTSTTDATVIQTRHRIPEASLHAGQVLVYQV 124 LKVIDQG+DDTTNA+SIR FF K A VA TT+T AT+IQTRHRIPE L AGQVLVYQV Sbjct: 66 LKVIDQGADDTTNAVSIRAFFKKVANVAVTTNTASATIIQTRHRIPEHPLTAGQVLVYQV 125 Query: 125 PIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATAYAYPVKVNARYVMDP 184 PIPEPLRFLEPRETETR+MHAL EYGLMHVKLYEDIA+ G IAT YAYPVKV RYVMDP Sbjct: 126 PIPEPLRFLEPRETETRKMHALEEYGLMHVKLYEDIAKHGRIATTYAYPVKVEGRYVMDP 185 Query: 185 SPTPKFDNPKMDNCAALQLFGAGREKRIYAIPPYTTVVSLDFEDHPFTRYRFNAPCALCG 244 SPTPKFDNPKM ALQLFGAGREKRIYA+PP+T VVSLDFEDHPF F+ PCALC Sbjct: 186 SPTPKFDNPKMHRSPALQLFGAGREKRIYAVPPFTDVVSLDFEDHPFEVQTFDQPCALCA 245 Query: 245 ADNSYLDEIVTDDKGGRMFVCSDTDFCEQRQAAGHHG 281 A+N YLDE++ DD GG MFVCSDTD CE+R+ GH G Sbjct: 246 AENVYLDEVILDDHGGHMFVCSDTDHCEKRREEGHRG 282 >phnJ [P] COG3627 Uncharacterized enzyme of phosphonate metabolism Eco Escherichia coli K12 Length = 281 Score = 454 bits (1168), Expect = e-127, Method: Compositional matrix adjust. Identities = 208/279 (74%), Positives = 236/279 (84%) Query: 2 NAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGP 61 N YNFAYLDEQTKRMIRRAILKA+AIPGYQVPF REMPMPYGWGTGG+Q+TA+++G Sbjct: 3 NLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGE 62 Query: 62 DDVLKVIDQGSDDTTNAISIRKFFGKTAGVATTTSTTDATVIQTRHRIPEASLHAGQVLV 121 DVLKVIDQG+DDTTNA+SIR FF + GV TT T DATVIQTRHRIPE L Q+++ Sbjct: 63 SDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATVIQTRHRIPETPLTEDQIII 122 Query: 122 YQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATAYAYPVKVNARYV 181 +QVPIPEPLRF+EPRETETR MHAL EYG+M VKLYEDIARFGHIAT YAYPVKVN RYV Sbjct: 123 FQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGRYV 182 Query: 182 MDPSPTPKFDNPKMDNCAALQLFGAGREKRIYAIPPYTTVVSLDFEDHPFTRYRFNAPCA 241 MDPSP PKFDNPKMD ALQLFGAGREKRIYA+PP+T V SLDF+DHPFT +++ PCA Sbjct: 183 MDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEPCA 242 Query: 242 LCGADNSYLDEIVTDDKGGRMFVCSDTDFCEQRQAAGHH 280 +CG+ +SYLDE+V DD G RMFVCSDTD+C Q+ A + Sbjct: 243 ICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEAKNQ 281 >ECs5081 [P] COG3627 Uncharacterized enzyme of phosphonate metabolism Ecs Escherichia coli O157:H7 Length = 281 Score = 454 bits (1167), Expect = e-127, Method: Compositional matrix adjust. Identities = 208/276 (75%), Positives = 235/276 (85%) Query: 2 NAPTYNFAYLDEQTKRMIRRAILKAIAIPGYQVPFASREMPMPYGWGTGGVQVTAAILGP 61 N YNFAYLDEQTKRMIRRAILKA+AIPGYQVPF REMPMPYGWGTGG+Q+TA+++G Sbjct: 3 NLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPMPYGWGTGGIQLTASVIGE 62 Query: 62 DDVLKVIDQGSDDTTNAISIRKFFGKTAGVATTTSTTDATVIQTRHRIPEASLHAGQVLV 121 DVLKVIDQG+DDTTNA+SIR FF + GV TT T DATVIQTRHRIPE L Q+++ Sbjct: 63 SDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDATVIQTRHRIPETPLTEDQIII 122 Query: 122 YQVPIPEPLRFLEPRETETRRMHALAEYGLMHVKLYEDIARFGHIATAYAYPVKVNARYV 181 +QVPIPEPLRF+EPRETETR MHAL EYG+M VKLYEDIARFGHIAT YAYPVKVN RYV Sbjct: 123 FQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYEDIARFGHIATTYAYPVKVNGRYV 182 Query: 182 MDPSPTPKFDNPKMDNCAALQLFGAGREKRIYAIPPYTTVVSLDFEDHPFTRYRFNAPCA 241 MDPSP PKFDNPKMD ALQLFGAGREKRIYA+PP+T V SLDF+DHPFT +++ PCA Sbjct: 183 MDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPPFTRVESLDFDDHPFTVQQWDEPCA 242 Query: 242 LCGADNSYLDEIVTDDKGGRMFVCSDTDFCEQRQAA 277 +CG+ +SYLDE+V DD G RMFVCSDTD+C Q+ A Sbjct: 243 ICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQSEA 278