BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0007.p (207 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc02275 [Q] COG1335 Amidases related to nicotinamidase Sme Sino... 295 3e-80 mlr4882 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesor... 286 2e-77 BMEI0546 [Q] COG1335 Amidases related to nicotinamidase Bme Bruc... 271 1e-72 RSc1760 [Q] COG1335 Amidases related to nicotinamidase Rso Ralst... 248 6e-66 VCA0712 [Q] COG1335 Amidases related to nicotinamidase Vch Vibri... 197 1e-50 >SMc02275 [Q] COG1335 Amidases related to nicotinamidase Sme Sinorhizobium meliloti Length = 211 Score = 295 bits (756), Expect = 3e-80, Method: Compositional matrix adjust. Identities = 141/197 (71%), Positives = 157/197 (79%) Query: 7 DDLLLVIDVQNDFCPGGALAVADGDAVVPVVNRLAARFDHVVLTQDWHPAGHSSFATSHP 66 D+ L+VID+QNDFCPGGALAV GD +VP VNRL HVVLTQDWHPAGHSSFA++HP Sbjct: 15 DEALIVIDMQNDFCPGGALAVEGGDEIVPAVNRLIDASPHVVLTQDWHPAGHSSFASTHP 74 Query: 67 GAAPFSEIAMPYGRQTLWPDHCIQGSAGAAFHPDLAIDRAELVIRKGFRVAIDSYSAFFE 126 G APF +AMPYG QTLWP+HC+QGSAGA FHP L AELVIRKGFR IDSYSAFFE Sbjct: 75 GKAPFQTVAMPYGEQTLWPEHCVQGSAGADFHPSLRWTSAELVIRKGFRREIDSYSAFFE 134 Query: 127 NDRKTPTGLAGYLRERGLRRVVMAGLATDFCVQYSALDARRLGFETTVVLAGCRAIDLGG 186 ND +TPTGLAGYLRERG+R V + GLATDFCV +SALDA GF T+VVL CR IDL G Sbjct: 135 NDHRTPTGLAGYLRERGIRSVTLCGLATDFCVAFSALDAVAKGFSTSVVLGACRGIDLNG 194 Query: 187 SLAVATAAMRAAGVQLV 203 SLA T MR AGV+L+ Sbjct: 195 SLAAMTLRMRDAGVRLI 211 >mlr4882 [Q] COG1335 Amidases related to nicotinamidase Mlo Mesorhizobium loti Length = 204 Score = 286 bits (732), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 139/200 (69%), Positives = 154/200 (77%) Query: 7 DDLLLVIDVQNDFCPGGALAVADGDAVVPVVNRLAARFDHVVLTQDWHPAGHSSFATSHP 66 D L+VID+QNDFCPGGALAV GD +VP+VN + R DHVVLTQDWHPAGHSSFA+SHP Sbjct: 3 DQALVVIDLQNDFCPGGALAVTGGDEIVPLVNDMIRRTDHVVLTQDWHPAGHSSFASSHP 62 Query: 67 GAAPFSEIAMPYGRQTLWPDHCIQGSAGAAFHPDLAIDRAELVIRKGFRVAIDSYSAFFE 126 GA PF+ I MPYG+QTLWPDHCIQGS G+ FH LA +AELVIRKGFR IDSYSAFFE Sbjct: 63 GAQPFTMIEMPYGQQTLWPDHCIQGSLGSDFHSGLAWTKAELVIRKGFRPDIDSYSAFFE 122 Query: 127 NDRKTPTGLAGYLRERGLRRVVMAGLATDFCVQYSALDARRLGFETTVVLAGCRAIDLGG 186 ND TPTGL GYLRERG+ V + GLATDFCV +SALDA GF+TTV L CR IDL G Sbjct: 123 NDHTTPTGLGGYLRERGIDTVTLVGLATDFCVGFSALDAVSQGFKTTVRLDACRGIDLNG 182 Query: 187 SLAVATAAMRAAGVQLVEEL 206 SL MR AGV L + L Sbjct: 183 SLEAMLRRMRDAGVALEDHL 202 >BMEI0546 [Q] COG1335 Amidases related to nicotinamidase Bme Brucella melitensis Length = 251 Score = 271 bits (692), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 124/198 (62%), Positives = 149/198 (75%) Query: 10 LLVIDVQNDFCPGGALAVADGDAVVPVVNRLAARFDHVVLTQDWHPAGHSSFATSHPGAA 69 L+VIDVQNDFCPGGALAV GD ++P+VNRL ++VV+TQDWHPA HSSFA++H G+ Sbjct: 48 LVVIDVQNDFCPGGALAVERGDEIIPIVNRLIGESENVVVTQDWHPANHSSFASNHSGSR 107 Query: 70 PFSEIAMPYGRQTLWPDHCIQGSAGAAFHPDLAIDRAELVIRKGFRVAIDSYSAFFENDR 129 PF + M YG Q LWPDHC+Q S GA FHPDL RA+LV+RKGFR+ IDSYSAFFEND Sbjct: 108 PFDTVNMSYGPQVLWPDHCVQNSEGAEFHPDLQWWRAQLVVRKGFRIGIDSYSAFFENDH 167 Query: 130 KTPTGLAGYLRERGLRRVVMAGLATDFCVQYSALDARRLGFETTVVLAGCRAIDLGGSLA 189 TPTGL GYLRER + + M GLATDFCV SALDA + GF+ V L CR IDL GS+ Sbjct: 168 CTPTGLGGYLRERNIGSLTMVGLATDFCVASSALDAVQQGFQVRVRLDACRGIDLNGSMN 227 Query: 190 VATAAMRAAGVQLVEELV 207 + M+ AGV+L++E V Sbjct: 228 IMLEKMKQAGVELIDEPV 245 >RSc1760 [Q] COG1335 Amidases related to nicotinamidase Rso Ralstonia solanacearum Length = 210 Score = 248 bits (633), Expect = 6e-66, Method: Compositional matrix adjust. Identities = 125/204 (61%), Positives = 143/204 (70%) Query: 2 MKIAPDDLLLVIDVQNDFCPGGALAVADGDAVVPVVNRLAARFDHVVLTQDWHPAGHSSF 61 M D LLV+DVQNDF PGGALAV DGD V+PV+NRLA F VVLTQDWHP H SF Sbjct: 1 MHPTATDCLLVMDVQNDFLPGGALAVPDGDRVIPVINRLARAFGRVVLTQDWHPRDHVSF 60 Query: 62 ATSHPGAAPFSEIAMPYGRQTLWPDHCIQGSAGAAFHPDLAIDRAELVIRKGFRVAIDSY 121 A +HPGA PF I +PYG+Q LWP HC+Q S GAA L + A+L++RKG+ IDSY Sbjct: 61 AANHPGAQPFGTIDLPYGQQVLWPVHCVQHSTGAALADALDVPHAQLIVRKGYHRHIDSY 120 Query: 122 SAFFENDRKTPTGLAGYLRERGLRRVVMAGLATDFCVQYSALDARRLGFETTVVLAGCRA 181 SAFFE DR TPTGL GYLRE G+R V AGLATDFCV +SALDAR G E V+ CRA Sbjct: 121 SAFFEADRTTPTGLLGYLRELGIRHVFCAGLATDFCVAWSALDARAAGLEVVVIEDACRA 180 Query: 182 IDLGGSLAVATAAMRAAGVQLVEE 205 IDL GSLA A M AG+ ++ Sbjct: 181 IDLDGSLAQAWQRMTDAGIARLQS 204 >VCA0712 [Q] COG1335 Amidases related to nicotinamidase Vch Vibrio cholerae Length = 206 Score = 197 bits (501), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 98/195 (50%), Positives = 129/195 (66%), Gaps = 2/195 (1%) Query: 10 LLVIDVQNDFCPGGALAVADGDAVVPVVNRLAARFDHVVLTQDWHPAGHSSFATSHPGAA 69 LL++DVQNDF P GAL V DGDAVVPV+N+L F+HV+ T+DWHPA H+SFA+ G Sbjct: 5 LLLVDVQNDFSPSGALPVPDGDAVVPVINQLLPLFEHVIATKDWHPAKHASFASVQ-GKT 63 Query: 70 PFSEIAMPYGRQTLWPDHCIQGSAGAAFHPDLAIDRAELVIRKGFRVAIDSYSAFFENDR 129 P I + Q +WPDHC+Q + GA F L + R ++ KG + +DSYS FF+N R Sbjct: 64 PGEVIDLNGIPQVMWPDHCVQNTHGAEFIAGLDVQRITHIVTKGTHLDVDSYSGFFDNQR 123 Query: 130 KTPTGLAGYLRERGLRRVVMAGLATDFCVQYSALDARRLGFETTVVLAGCRAIDLG-GSL 188 TGLA YL +G+ V +AGLATD+CV+Y+ALDA L F+T V+ CR ++L G Sbjct: 124 FHATGLAEYLSNQGISEVYIAGLATDYCVKYTALDAVSLYFKTWVIQDACRGVELNAGDC 183 Query: 189 AVATAAMRAAGVQLV 203 A A AM+ AG QL+ Sbjct: 184 ATAWHAMQTAGCQLI 198