BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0254.0006.p (273 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0958 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mac M... 201 2e-51 MTH973 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mth M... 135 1e-31 MJ0915 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mja M... 130 4e-30 PA3505 [R] COG1712 Predicted dinucleotide-utilizing enzyme Pae P... 129 5e-30 RSp0722 Non COG 124 3e-28 >MA0958 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mac Methanosarcina acetivorans str.C2A Length = 271 Score = 201 bits (510), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 109/269 (40%), Positives = 166/269 (61%), Gaps = 5/269 (1%) Query: 10 RVAVAGLGAIGTDVAKALDQGIEGLTLVAVSANSPDKHRGWVAELKSA-PKLVPIEQLAE 68 ++ + G G IG + +A+D G L A+ +S K A LK+ P + IE+L Sbjct: 3 KIGIVGCGFIGGQICRAIDGGEVSAELYALCDSSESKALELAASLKTCKPSYMKIEELIR 62 Query: 69 VADIVVECAPSKLVRSIVAPVVERGKTAVVLSVGALLQNE---DLVDLARAHGGQIIVPT 125 D+++E A VR IV ++ G ++LSVGAL E L LA+ H ++ P+ Sbjct: 63 GVDLIIESASQNAVRFIVPQALKAGCDVMILSVGALADEELRDTLFGLAKEHNCKLYFPS 122 Query: 126 GALIGLDAVTAAAVGTIHSAKLVTRKPVAGLVGAPHLAENNIQIEGITEPLRIFEGSARD 185 GA++G+D + +A+ I S L TRKP AGL+GAP++ E+ I++E + + +FEG A + Sbjct: 123 GAVVGIDGLNSASAAGISSVTLSTRKPPAGLMGAPYVVEHGIELEKLEKKTVLFEGPASE 182 Query: 186 AAKGFPANLNVAVALSLAGIGPDRTRVEIWADPTVTRNTHRIEVDSDSARFSMMIENIPS 245 A K FPAN+NVA +SLAGIG +RTRV++ ADP++ RN H I V+ + +FS +EN+PS Sbjct: 183 AVKAFPANVNVAATISLAGIGFERTRVKVIADPSLFRNVHEIIVEGEFGKFSTRVENLPS 242 Query: 246 -ENPKTGRITALSVIACLRKLRASLRIGT 273 ENPKT + ALS ++ L+K+ ++IGT Sbjct: 243 PENPKTSYLAALSAVSTLKKILNPVQIGT 271 >MTH973 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mth Methanothermobacter thermautotrophicus Length = 257 Score = 135 bits (339), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 94/217 (43%), Positives = 123/217 (56%), Gaps = 25/217 (11%) Query: 64 EQLAEVADIVVECAPSKLVRSIVAPVVERGKTAVVLSVGALLQ---NEDLVDLARAHGGQ 120 + L EV D+VVE A + VR +V ++E GK VV+SVGAL+ E LV+LA + Sbjct: 59 DMLPEV-DLVVEAASPEAVRDLVPEILEAGKDVVVMSVGALMDPELREMLVELASLNDAT 117 Query: 121 IIVPTGALIGLDAVTAAAVGTIHSAKLVTRKPVAGLVGAPHLAENNIQIEGIT--EPLRI 178 I VP+GA++GLD + AA++G I S KL+TRKP L GI+ E + Sbjct: 118 IHVPSGAIVGLDGLKAASMGNIESVKLITRKPPRSL--------------GISMDEKKVL 163 Query: 179 FEGSARDAAKGFPANLNVAVALSLAGIGPDR-TRVEIWADPTVTRNTHRIEVDSDSARFS 237 + G A +A K FP N+NVA ALSLA DR VEI ADP V RN H + V D F Sbjct: 164 YRGRASEAVKKFPLNINVAAALSLAC---DRDVDVEIIADPAVDRNVHEVTVRGDFGEFK 220 Query: 238 MMIENI-PSENPKTGRITALSVIACLRKLRASLRIGT 273 + EN+ S NPKT + A S I L+ L ++ IGT Sbjct: 221 TITENVRCSVNPKTSVMAAYSAIKLLKSLSENIHIGT 257 >MJ0915 [R] COG1712 Predicted dinucleotide-utilizing enzyme Mja Methanococcus jannaschii Length = 267 Score = 130 bits (327), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 99/270 (36%), Positives = 140/270 (51%), Gaps = 11/270 (4%) Query: 10 RVAVAGLGAIGTDVAK-ALDQGIEGLTLVAVSANSPDKHRGWVAELKSAPKLVPIEQLA- 67 ++ + G GAIG + K LD I+ + AV + DK + ++E A I+ L Sbjct: 3 KIGIVGCGAIGNFITKKVLDGTIKNAKISAVYDRNFDKAK-TLSERTGAKICSSIDDLVK 61 Query: 68 EVADIVVECAPSKLVRSIVAPVVERGKTAVVLSVGALLQNE---DLVDLARAHGGQIIVP 124 E D+VVE A K V I + K +++SVGAL + L DLA+ G +I +P Sbjct: 62 EDLDLVVEAASIKAVEEIAEKSLINNKDVLIMSVGALADKKLFLKLRDLAKTVGRKIYLP 121 Query: 125 TGALIGLDAVTAAAVGTIHSAKLVTRKPVAGLVGAPHLAENNIQIEGITEPLRIFEGSAR 184 +GA+ GLDA+ A +G I L T KPVA L A L + E I P+ +FEG Sbjct: 122 SGAIGGLDAIKALRLGEIEEVVLKTTKPVAALEDA--LKNLGYKPEDIKNPVIVFEGDVF 179 Query: 185 DAAKGFPANLNVAVALSLAGIGPDRTRVEIWADPTVTRNTHRIEVDSDSARFSMMIENIP 244 A K FPAN+NV+V LS+A P +V I ADP N H + V S + IEN+P Sbjct: 180 KAIKEFPANINVSVTLSIAAEFP--AKVVIVADPNAKLNKHELFVKSSIGTLRVCIENVP 237 Query: 245 -SENPKTGRITALSVIACLRKLRASLRIGT 273 ENP+T + A S + +R L +++GT Sbjct: 238 FEENPRTSALAAYSAVRLIRDLAEPVKVGT 267 >PA3505 [R] COG1712 Predicted dinucleotide-utilizing enzyme Pae Pseudomonas aeruginosa Length = 267 Score = 129 bits (325), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 89/257 (34%), Positives = 140/257 (54%), Gaps = 7/257 (2%) Query: 11 VAVAGLGAIGTDVAKALDQGIE-GLTLVAVSANSPDKHRGWVAELKSAPKLVPIEQLAEV 69 + + G GAIG V + L+ + + V V +S + R +A L+ P+++ E Sbjct: 4 IVMIGCGAIGAGVLELLENDPQLRVDAVIVPRDSETQVRHRLASLRRPPRVLSALPAGER 63 Query: 70 ADIVVECAPSKLVRSIVAPVVERGKTAVVLSVGALLQN---EDLVDLARAHGGQIIVPTG 126 D++VECA + + V P + +G +V+SVGAL + E L A+A G +I + G Sbjct: 64 PDLLVECAGHRAIEQHVLPALAQGIPCLVVSVGALSEPGLVERLEAAAQAGGSRIELLPG 123 Query: 127 ALIGLDAVTAAAVGTIHSAKLVTRKPVAGLVGAPHLAENNIQIEGITEPLRIFEGSARDA 186 A+ +DA++AA VG + S + RKP + +G P E ++ + + IF+GSAR+A Sbjct: 124 AIGAIDALSAARVGGLESVRYTGRKPASAWLGTP--GETVCDLQRLEKARVIFDGSAREA 181 Query: 187 AKGFPANLNVAVALSLAGIGPDRTRVEIWADPTVTRNTHRIEVDSDSARFSMMIENIP-S 245 A+ +P N NVA LSLAG+G DRT+V + ADP N H++E F + + P + Sbjct: 182 ARLYPKNANVAATLSLAGLGLDRTQVRLIADPESCENVHQVEASGAFGGFELTLRGKPLA 241 Query: 246 ENPKTGRITALSVIACL 262 NPKT +T SV+ L Sbjct: 242 ANPKTSALTVYSVVRAL 258 >RSp0722 Non COG Length = 268 Score = 124 bits (310), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 92/261 (35%), Positives = 134/261 (51%), Gaps = 12/261 (4%) Query: 10 RVAVAGLGAIGTDVAKALDQGIE-GLTLVAVSANSPDKHRGWVAELKSAPKLVPIEQL-- 66 V++ G GAIG V + L + V V + D+ R +A AP+ + +L Sbjct: 3 HVSMVGCGAIGQGVLELLKSDPDLCFDTVIVPEHGMDRARAAIAPF--APRTRVMTRLPA 60 Query: 67 -AEVADIVVECAPSKLVRSIVAPVVERGKTAVVLSVGALLQ---NEDLVDLARAHGGQII 122 A+ D++VECA +R V P +E+G +V+SVGAL + E L AR Q+ Sbjct: 61 QADRPDLLVECAGHDALREHVVPALEQGIDCLVVSVGALSEPGLAERLEAAARRGHAQMQ 120 Query: 123 VPTGALIGLDAVTAAAVGTIHSAKLVTRKPVAGLVGAPHLAENNIQIEGITEPLRIFEGS 182 + +GA+ +DA+ AA VG + + RKP G P AE ++ + IFEG Sbjct: 121 LLSGAIGAIDALAAARVGGLDAVVYTGRKPPRAWKGTP--AERQFDLDALDRTTVIFEGK 178 Query: 183 ARDAAKGFPANLNVAVALSLAGIGPDRTRVEIWADPTVTRNTHRIEVDSDSARFSMMIEN 242 A DAA FP N NVA L+LAG+G +RT V + ADPT+ N H +E F +++ Sbjct: 179 ASDAALLFPKNANVAATLALAGLGMERTHVRLLADPTIDENIHHVEARGAFGGFELIMRG 238 Query: 243 IP-SENPKTGRITALSVIACL 262 P + NPKT +T SV+ L Sbjct: 239 KPLAANPKTSALTVFSVVRAL 259