BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0016.p (286 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the T... 217 3e-56 SA2366 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 211 1e-54 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 211 2e-54 lin0862 [R] COG2159 Predicted metal-dependent hydrolase of the T... 196 4e-50 mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the T... 64 3e-10 >AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 327 Score = 217 bits (552), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 120/297 (40%), Positives = 173/297 (58%), Gaps = 21/297 (7%) Query: 4 QLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEI-AVDLTKRVNDRLAEACARHPGR 62 +L +I DTRL+ MD GI+ +LS APA Q + + A+++ +R ND LAE CA+ P R Sbjct: 35 RLLDIQDTRLKLMDAHGIETMILSLNAPAVQAIPDKTKAIEIARRANDALAEECAKRPDR 94 Query: 63 FAAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNG------LFIDDKRFWPIFEVA 116 F AFAALP P+ A +EL RCVN+L F GA+++G + L+ D ++ P + Sbjct: 95 FLAFAALPLQDPDAATQELRRCVNDLGFVGALVNGFSQEGDGQTPLYYDLPQYRPFWGEV 154 Query: 117 EKLDVPIYLHPSVPHSEVTKYYYEDYARDFPTVIRPAWGFTVETATAAIRLVLSRALEKY 176 EKLDVP YLHP P + ++ Y P ++ P W F ETA A+RL+ S +++ Sbjct: 155 EKLDVPFYLHPRNPLPQDSRIY-----DGHPWLLGPTWAFAQETAVHALRLMASGLFDEH 209 Query: 177 PRLQIILGHFGETLPFLLWRIDQALA-------RPGQEGLGFRRQFSQHFHVTCSGFFST 229 PRL IILGH GE LP+++WRID A P + F F+++FH+T SG F T Sbjct: 210 PRLDIILGHMGEGLPYMMWRIDHRNAWVKLPPRYPAKR--RFVDYFNENFHITTSGNFRT 267 Query: 230 PALTCTMLELGIDRIMFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 L +LE+G DRI+F+ D+P+ + +W ++ D+ K+ NARRL KL Sbjct: 268 QTLIDAILEIGADRILFSTDWPFENIDHASDWFHATSIAEADRVKIGRTNARRLFKL 324 >SA2366 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sau Staphylococcus aureus N315 Length = 336 Score = 211 bits (538), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 112/291 (38%), Positives = 175/291 (60%), Gaps = 12/291 (4%) Query: 2 ADQLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPG 61 AD+L+ D R++ M+ + +QVLS+G + L + A++L ++ ND+LA A++P Sbjct: 48 ADELSH-HDERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIAQYPN 106 Query: 62 RFAAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDV 121 RF FA LP PE AARE ERC+N+L FKGA+I G F+D ++ IF+ AE LDV Sbjct: 107 RFVGFATLPINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKTAENLDV 166 Query: 122 PIYLHPSVPHSEVTKYYYEDYARDFPTVIRP-----AWGFTVETATAAIRLVLSRALEKY 176 PIYLHP+ +S++ + YY+ ++P V +G+ ++ AI LVLS ++Y Sbjct: 167 PIYLHPAPVNSDIYQSYYKG---NYPEVTAATFACFGYGWHIDVGIHAIHLVLSGIFDRY 223 Query: 177 PRLQIILGHFGETLPFLLWRIDQAL-ARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCT 235 P+L +I+GH+GE +PF L R+D+AL A + + F F++T SG + P Sbjct: 224 PKLNMIIGHWGEFIPFFLERMDEALFAEHLNHSVSYY--FKNSFYITPSGMLTKPQFDLV 281 Query: 236 MLELGIDRIMFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 E+GIDRI++A DYPY++ + ++ EL L+ ++K K+ N +LL L Sbjct: 282 KKEVGIDRILYAADYPYIEPEKLGVFLDELGLTDEEKEKISYTNGAKLLGL 332 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 211 bits (536), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 109/292 (37%), Positives = 165/292 (56%), Gaps = 12/292 (4%) Query: 1 VADQLAEIGDTRLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHP 60 + ++L ++G R+ MD GID+QV+S +P Q PE+A L ++ ND+L+EA HP Sbjct: 51 IHERLIDLGAGRIAHMDATGIDIQVISITSPGVQVFEPELATTLARQANDQLSEAIKNHP 110 Query: 61 GRFAAFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLD 120 RFA AA+ P+ AA E+ER + +L G +I+ T G ++DD+++W IF AE Sbjct: 111 DRFAGLAAVAPQSPQTAALEIERAIQKLGLCGVLINSHTGGEYLDDQKYWEIFAAAEANS 170 Query: 121 VPIYLHPSVPHSEVTKYYYEDYARDFPTVIRPAWGFTVETATAAIRLVLSRALEKYPRLQ 180 PIYLHP P E+ + DY F WGFTVETAT +RL++ + +P+L Sbjct: 171 APIYLHPRTPSPEMISPFL-DYGLYF-----AGWGFTVETATHGLRLIMGGVFDHFPKLT 224 Query: 181 IILGHFGETLPFLLWRIDQALARPGQEGLGFR------RQFSQHFHVTCSGFFSTPALTC 234 I+LGH GE LP+ L R+D + + G + F HF +T SG S PAL Sbjct: 225 IVLGHMGEGLPYWLQRLDNRYSLQVEIGAVPKMPRLPSEYFKDHFLITTSGVCSPPALRH 284 Query: 235 TMLELGIDRIMFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 + LG D ++FA DYPY ++ ++++ +S ++RK+ NA +L +L Sbjct: 285 VLEVLGSDAVLFAADYPYESVEEAVSFLDNAPISDQERRKIYQTNAEKLFRL 336 >lin0862 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Lin Listeria innocua Length = 332 Score = 196 bits (499), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 110/285 (38%), Positives = 160/285 (56%), Gaps = 5/285 (1%) Query: 7 EIGDT--RLRAMDEAGIDVQVLSHGAPAAQKLRPEIAVDLTKRVNDRLAEACARHPGRFA 64 E+ DT RL MD+ GID+QVLS+G + Q L P+I+V L KR ND LA+ ++P RFA Sbjct: 48 EMQDTEKRLAFMDQHGIDMQVLSYGNSSPQNLGPKISVALCKRANDELAKVIQQNPTRFA 107 Query: 65 AFAALPTPVPEQAARELERCVNELKFKGAMIHGLTNGLFIDDKRFWPIFEVAEKLDVPIY 124 FA LP PE AA EL+R V EL FKGA++ G + D+ ++PIFE+AEKLDVPI Sbjct: 108 GFAVLPVGSPEDAAIELKRAVEELGFKGALVKGQFENKYFDNSFYYPIFEMAEKLDVPIS 167 Query: 125 LHPS-VPHSEVTKYYYEDYARDFPTVIRPAWGFT--VETATAAIRLVLSRALEKYPRLQI 181 HPS +P +Y+ D D T + + GF ++ +R++LS +K P L+I Sbjct: 168 FHPSFIPEPITEQYFASDAWSDVVTGVFSSAGFGWHMDVGIQVVRMILSGIFDKLPNLKI 227 Query: 182 ILGHFGETLPFLLWRIDQALARPGQEGLGFRRQFSQHFHVTCSGFFSTPALTCTMLELGI 241 I GH GE +P L R+D L + + +VT SG + EL Sbjct: 228 ITGHLGEMVPMFLNRMDDTLGHWTTLERKISDYYRTNVYVTPSGLLYKNEWKFLLNELDE 287 Query: 242 DRIMFAVDYPYVDNKDGMNWIRELQLSIDDKRKLVGNNARRLLKL 286 + +++A+DYP+V ++ ++ L L+ + K K+ NA +LL L Sbjct: 288 NHLIYALDYPFVKTENAGTFLDTLDLTDEVKAKIAHENAEKLLHL 332 >mlr9221 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mlo Mesorhizobium loti Length = 357 Score = 64.3 bits (155), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 68/258 (26%), Positives = 121/258 (46%), Gaps = 31/258 (12%) Query: 39 EIAVDLTKRVNDRL-AEACARHPGRFAAFAALPTPVPEQAARELERCVNELKFKGAMIHG 97 +++ L + +N+ + AE R P R A +P PE A E+E +L+F ++ Sbjct: 107 DLSAALCRAINNWISAEWLDRDP-RLRASIVVPVHSPELAVAEIEHRAADLRFVQVLMLA 165 Query: 98 LTNGLFIDDKRFWPIFEVAEKLDVPIYLHPSV----PHSEV--TKYYYEDYARDFPTVIR 151 + + + ++ WPI+ AE+ D+PI +H P S + + +Y +DY P Sbjct: 166 MGE-VPLGRRQNWPIYAAAERHDLPIGIHAGSSLRNPPSPLGWSSHYIDDYVSQAP---- 220 Query: 152 PAWGFTVETATAAIRLVLSRALEKYPRLQIILGHFGET-LPFLLWRIDQALARPGQEGLG 210 GF+ A LV+ K+PRL+++L G T LP LWR ++ E Sbjct: 221 ---GFS----GALNSLVVEGVFNKFPRLKVVLLESGVTWLPAHLWRANKQWRGVRSETPW 273 Query: 211 FRRQFS----QHFHVTCSGFFSTPA---LTCTMLELGIDR-IMFAVDYPYVDNKDGMNWI 262 R S +H +T F + P + + ++G D ++F+ DYP+ + DG + + Sbjct: 274 LTRPPSEIIREHVRLTVQPFDAPPTDGQVQTIIDQIGSDEMLLFSTDYPHW-HFDGTDAV 332 Query: 263 RELQLSIDDKRKLVGNNA 280 + LS + K++ +NA Sbjct: 333 PQ-GLSHESLSKMLADNA 349