BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0022.p (253 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 143 3e-34 SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown ... 139 8e-33 slr0315 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 132 8e-31 MA0479 [IQR] COG1028 Dehydrogenases with different specificities... 128 1e-29 alr5286 [IQR] COG1028 Dehydrogenases with different specificitie... 127 2e-29 >SMa0320 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sme Sinorhizobium meliloti Length = 249 Score = 143 bits (361), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 90/249 (36%), Positives = 133/249 (53%), Gaps = 9/249 (3%) Query: 1 MTNNGKRVAWVTGGGTGIGESGAEFLAADGWTVVVSGRRKEELDRVVANITKKGGKAEAI 60 MT+ ++A VTG +GIG + A LA G V ++ RR ++L+ + I KGG+A I Sbjct: 1 MTSLNGKIALVTGASSGIGAATAAKLAEAGAKVGIAARRTDKLEDLKKKIEAKGGEALVI 60 Query: 61 PLDVSNKTDVNKAAETIVGKHGRIDLLVNSAGINVPKRSWADMELEGWDKLVEINLNGVL 120 +DV + T V + +V +G ID+LVN+AG+ +P +++ W ++V++N+ G+L Sbjct: 61 EMDVVDTTSVEAGVKKLVDAYGSIDILVNNAGL-MPLSDIDQFKVDEWQRMVDVNVKGLL 119 Query: 121 YCMRAVLPTMRKQKDGCIINVASWAGRHVSKMPGPAYTTTKHAVLALTHSFNMDECV-NG 179 AVLP M KQ G + N++S AGR V K Y TKHAV A + M+ +G Sbjct: 120 NTTAAVLPQMIKQHSGHVFNMSSIAGRKVFKGL-SVYCATKHAVTAFSDGLRMEVGQKHG 178 Query: 180 LRACCLSPGEVATPILKQ------RPVVPSEAEQAKMLQPEDCGRTIAFVASMPARVCMN 233 +R C+ PG VAT + R + A Q LQ ED G TI F A PA V + Sbjct: 179 IRVTCIQPGAVATELYDHITDPGYRQQMDELATQMTFLQGEDIGDTIVFAAQAPAHVDVA 238 Query: 234 EILISPTHN 242 E+ + P Sbjct: 239 ELFVLPVEQ 247 >SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sau Staphylococcus aureus N315 Length = 231 Score = 139 bits (349), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 80/239 (33%), Positives = 130/239 (54%), Gaps = 9/239 (3%) Query: 1 MTNNGKRVAWVTGGGTGIGESGAEFLAADGWTVVVSGRRKEELDRVVANITKKGGKAEAI 60 MT ++A VTG G+GIGE+ A L +G VV++GR K++L V + + K + Sbjct: 1 MTVLTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQNVANQLAQDSVKV--V 58 Query: 61 PLDVSNKTDVNKAAETIVGKHGRIDLLVNSAGINVPKRSWADMELEGWDKLVEINLNGVL 120 P DV+NK +V++ + G +D+++NSAG + + D +++ WD ++++N+ G L Sbjct: 59 PTDVTNKEEVDELMKIAQQTFGGLDIVINSAGQMLSSK-ITDYQVDEWDSMIDVNIKGTL 117 Query: 121 YCMRAVLPTMRKQKDGCIINVASWAGRHVSKMPGPAYTTTKHAVLALTHSFNMDECVNGL 180 Y +A LPTM +Q G +IN+AS +G V+K Y+ TK AV +T + G+ Sbjct: 118 YTAQAALPTMLEQSSGHLINIASISGFEVTK-SSTIYSATKAAVHTITQGLEKELAKTGV 176 Query: 181 RACCLSPGEVATPILKQRPVVPSEAEQAKMLQPEDCGRTIAFVASMPARVCMNEILISP 239 + +SPG V T I PS+ K L P+D + + + P V +NEI + P Sbjct: 177 KVTSISPGMVDTAITAAYN--PSD---RKKLDPQDIAEAVLYALTQPKHVNVNEITVRP 230 >slr0315 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Syn Synechocystis Length = 244 Score = 132 bits (332), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 84/244 (34%), Positives = 128/244 (52%), Gaps = 8/244 (3%) Query: 1 MTNNGKRVAWVTGGGTGIGESGAEFLAADGWTVVVSGRRKEELDRVVANITKKGGKAEAI 60 M N +V +TG +GIGE+ A+ LA +G VV+ GRR ++L++++ I GG AE Sbjct: 1 MLNIENKVIVITGASSGIGEATAKLLAQNGAKVVLGGRRIDKLEKLIKQIHASGGTAEFK 60 Query: 61 PLDVSNKTDVNKAAETIVGKHGRIDLLVNSAGINVPKRSWADMELEGWDKLVEINLNGVL 120 +DV+++ DV E K GR+D++ N+AG+ +P +++E WD ++ +N+ GVL Sbjct: 61 TVDVTDRHDVKAFVEFANDKFGRVDVIFNNAGV-MPLSPMNALKVEEWDNMINVNIRGVL 119 Query: 121 YCMRAVLPTMRKQKDGCIINVASWAGRHVSKMPGPAYTTTKHAVLALTHSFNMDECVNGL 180 + A LP M Q G IIN AS G HV Y TK+AV A++ + + Sbjct: 120 NGIAAGLPIMEAQGGGQIINTASI-GAHVVVPTAAVYCATKYAVWAISEGLRQES--QNI 176 Query: 181 RACCLSPGEVATP----ILKQRPVVPSEAEQAKMLQPEDCGRTIAFVASMPARVCMNEIL 236 R +SPG VAT I + E + L E R + + S P V +NEI+ Sbjct: 177 RVTTISPGVVATELGSDITDESSKGLLEELRKTALTSEAIARAVLYAVSQPDDVDVNEII 236 Query: 237 ISPT 240 + PT Sbjct: 237 VRPT 240 >MA0479 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mac Methanosarcina acetivorans str.C2A Length = 258 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 74/188 (39%), Positives = 106/188 (56%), Gaps = 2/188 (1%) Query: 7 RVAWVTGGGTGIGESGAEFLAADGWTVVVSGRRKEELDRVVANITKKGGKAEAIPLDVSN 66 +VA VTG GTG GES AE L A G +V + R LD + I G + I +DV + Sbjct: 15 KVAIVTGAGTGNGESIAERLYAGGASVALVSRHIAPLDDICNRIDSSGKRTFPIEVDVRD 74 Query: 67 KTDVNKAAETIVGKHGRIDLLVNSAGINVPKRS-WADMELEGWDKLVEINLNGVLYCMRA 125 V A +I+ + G+ID+ VN+AGI P + D+++E W ++E +L GV YCM+ Sbjct: 75 PHSVQIAVSSIIERFGKIDIAVNNAGITGPANTPLQDLDIEIWRDVIETDLTGVFYCMKY 134 Query: 126 VLPTMRKQKDGCIINVASWAGRHVSKMPGPAYTTTKHAVLALTHSFNMDECVNGLRACCL 185 +P M K G I+N++S G V AYTT KH V+ LT S ++ + +R C + Sbjct: 135 EIPAMLKNGSGAIVNMSSANGL-VGLAGMAAYTTAKHGVIGLTRSAALELAESNIRVCAV 193 Query: 186 SPGEVATP 193 +PG VATP Sbjct: 194 APGYVATP 201 >alr5286 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 255 Score = 127 bits (320), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 75/240 (31%), Positives = 132/240 (55%), Gaps = 3/240 (1%) Query: 1 MTNNGKRVAWVTGGGTGIGESGAEFLAADGWTVVVSGRRKEELDRVVANITKKGGKAEAI 60 M+ KR A +TG +GIG++ A A G V + R ++L VV + G A+A Sbjct: 15 MSLEQKRRALITGASSGIGKATALAFAKAGIDVALVSRSLDKLTPVVEATKQTGVVAKAY 74 Query: 61 PLDVSNKTDVNKAAETIVGKHGRIDLLVNSAGINVPKRSWADMELEGWDKLVEINLNGVL 120 +D+++ + V + I G ID+LVN+AG+ + ++ L W +++ +NL GV Sbjct: 75 TVDLADVSQVEAKIQAIAIDFGDIDILVNNAGVAYTA-TLSETPLADWQQVINLNLTGVF 133 Query: 121 YCMRAVLPTMRKQKDGCIINVASWAGRHVSKMPGPAYTTTKHAVLALTHSFNMDECVNGL 180 C++ +LPTMR + G IINV+S A + G Y+ +K ++AL+ + +E V+G+ Sbjct: 134 ECIKGILPTMRDRHTGTIINVSSIAAKQSFPHWG-VYSVSKAGLMALSQTLAQEERVHGI 192 Query: 181 RACCLSPGEVATPILKQRPVVPSEAEQAKMLQPEDCGRTIAFVASMPARVCMNEILISPT 240 R + PG V T L V ++ +++KML PE ++I + A +P + ++E+ + P+ Sbjct: 193 RVTAICPGAVNTG-LWDTETVKADFDRSKMLTPEIVAQSILYTALLPPQAVIDELTLMPS 251