BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0021.p (466 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2114 [GEPR] COG0477 Permeases of the major facilitator superfa... 133 8e-31 BS_yceI [GEPR] COG0477 Permeases of the major facilitator superf... 114 4e-25 YPO1932 [GEPR] COG0477 Permeases of the major facilitator superf... 114 5e-25 STM0356 [GEPR] COG0477 Permeases of the major facilitator superf... 114 6e-25 PM0835 [GEPR] COG0477 Permeases of the major facilitator superfa... 112 1e-24 >PA2114 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 423 Score = 133 bits (335), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 116/456 (25%), Positives = 200/456 (43%), Gaps = 46/456 (10%) Query: 18 SIDIGPLEQKHLQYANYRAVADTTEITKTHWHIATANALGWGFDGMDGVIFALISPMVIK 77 ++D GP + + YRA+ D E + W + +G+G DGMD + + + P +I Sbjct: 2 TLDAGPDASRDGHFGWYRAL-DKQE-RRAFW----SCKIGYGLDGMDTQMLSFVIPTLIA 55 Query: 78 EFSLSLPEYRSGMQIALFVGIAGLYFWPWLADRYGRRTLLAVNIALFSLLMPVAAMSPTF 137 + + E + L AG + L+DR GR L + + F+ + ++ + Sbjct: 56 LWGIGTGEAGFIHTMTLLASAAGGWIAGILSDRIGRVLTLQLTVLWFAFFTFLCGLAQNY 115 Query: 138 AVFVIARSLLFFALNGEWSLGSMLVAETWPARLRGRVISITRSAWCLG----ATLAGAIT 193 ++AR+L+ F GEW+ G++L+ E AR RG+ + + +S W +G A L + Sbjct: 116 EQLLVARTLMGFGFGGEWTAGAVLIGEVIKARDRGKAVGLVQSGWAIGWGLTAILYSLMF 175 Query: 194 GLVAANFGWRIAVMVPGVIALLAIYIRSTCPESPYWVRAQDRKRRISETLARGGTVSTED 253 L+ WR M+ + AL + +R E + A+ R + Sbjct: 176 SLLPPEEAWRALFMLGLLPALFVLVVRRLVKEPEIYREARQRNQ---------------- 219 Query: 254 SAWFGKAKSVGIRQVFMPDVLPATLVALFVACASTCIYGTVGAWMPLYLATEKHWSTAEY 313 A V ++F P +L TL A + + Y + AW+P +L TE+ + Sbjct: 220 -----GADKVSFYEIFAPGMLSITLRASLLTTGALGGYYAITAWLPTFLKTERGLTVLGT 274 Query: 314 SLFYVFYGLCGFLGLCLVGWLIDKIGRRRTFIITLIEGAIFMTLWVYSE----DRVLLWA 369 S + L ++G + +L D IGR++ F++ + F + +Y++ + +LW Sbjct: 275 SGYLAMVILGSYVGYVVSAFLTDLIGRKKNFVLFAV--GSFTVVLLYTQLPVSNGAMLW- 331 Query: 370 FGLAWCLGFLG---FWGPSTTLTAEIFPTRIRGAANGVVWAIAYFVGFVLFPFVSIALQQ 426 L + LGF F G + LT E+FPTRIRG+ G + + LFP + L Q Sbjct: 332 --LGFPLGFFASGIFSGMGSFLT-ELFPTRIRGSGQGFCYNAGRAIA-ALFPMLIGMLGQ 387 Query: 427 HTGSFALSFLCIPVLMIAMAIGVFLFVPEHTGKELN 462 S L + + I L +PE GK+L Sbjct: 388 KL-SLGLGIGAFAAVSYGVVILAALSLPETRGKQLE 422 >BS_yceI [GEPR] COG0477 Permeases of the major facilitator superfamily Bsu Bacillus subtilis Length = 400 Score = 114 bits (286), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 114/409 (27%), Positives = 186/409 (45%), Gaps = 35/409 (8%) Query: 56 LGWGFDGMDGVIFALISPMVIKEFSLSLPEYRSGMQIALFVGIA-GLYFWPWLADRYGRR 114 LGW FD MD I + I + E++LS PE + +G+A G + + LADR GR+ Sbjct: 18 LGWLFDAMDVGILSFIIAALHVEWNLS-PEEMKWIGSVNSIGMAAGAFLFGLLADRIGRK 76 Query: 115 TLLAVNIALFSLLMPVAAMSPTFAVFVIARSLLFFALNGEWSLGSMLVAETWPARLRGRV 174 + + + FS+ ++A + + F+I R ++ L GE + S LV+E RGRV Sbjct: 77 KVFIITLLCFSIGSGISAFVTSLSAFLILRFVIGMGLGGELPVASTLVSEAVVPEKRGRV 136 Query: 175 ISITRSAWCLGATLAGAITGLVAANFGWRIAVMVPGVIALLAIYIRSTCPESPYWVRAQD 234 I + S W +G A I+ V +FGW+ A+++ + A A+Y+R++ P+SP + Sbjct: 137 IVLLESFWAVGWLAAALISYFVIPSFGWQAALLLTALTAFYALYLRTSLPDSPKYESLSA 196 Query: 235 RKRRISETLARGGTVSTEDSAWFGKAKSVGIRQVFMPDVLPATLVALFVACASTCIYGTV 294 +KR + E + KSV RQ P V ++++ C YG Sbjct: 197 KKRSMWENV-----------------KSVWARQYIRPTV----MLSIVWFCVVFSYYGMF 235 Query: 295 GAWMP--LYLATEKHWSTAEYSLFYVFYGLCGFLGLCLVGWLIDKIGRRRTFIITLIEGA 352 W+P + L + EY L L G+ WLI+K GR+ ++ LI A Sbjct: 236 -LWLPSVMLLKGFSMIQSFEYVLLMTLAQLPGYFS---AAWLIEKAGRKWILVVYLIGTA 291 Query: 353 IFMTLWVYSEDRVLLWAFGLAWCLGFLGFWGPSTTLTAEIFPTRIRGAANGVVWAIAYFV 412 + ++ LL G+ LG WG T E +PT IR +G A + Sbjct: 292 GSAYFFGTADSLSLLLTAGVLLSFFNLGAWGVLYAYTPEQYPTAIRATGSGTTAAFGR-I 350 Query: 413 GFVLFPFV--SIALQQHTGSFALSFLCIPVLMIAMAIGVFLFVPEHTGK 459 G + P + ++A + + S S CI +L +A+ L + + T + Sbjct: 351 GGIFGPLLVGTLAARHISFSVIFSIFCIAIL---LAVACILIMGKETKQ 396 >YPO1932 [GEPR] COG0477 Permeases of the major facilitator superfamily Ype Yersinia pestis Length = 409 Score = 114 bits (285), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 110/440 (25%), Positives = 190/440 (43%), Gaps = 53/440 (12%) Query: 41 TEITKTHWHIATANALGWGFDGMDGVIFALISPMVIKEFSLSLPEYRSGMQIALFVGIAG 100 +++ +T A GW D +D ++F + ++ + + + + L G Sbjct: 5 SDLNQTERKTFIAAFGGWALDALDFMVFTFVIATLMALWQIDAGQAGMLSTVTLLFSAIG 64 Query: 101 LYFWPWLADRYGRRTLLAVNIALFSLLMPVAAMSPTFAVFVIARSLLFFALNGEWSLGSM 160 + LADRYGR +L + I FSL + + F I R+L GEW++GS+ Sbjct: 65 GWGAGILADRYGRVRILQITILWFSLCTVLIGFAQNFEHVFILRALQGLGFGGEWAVGSV 124 Query: 161 LVAETWPARLRGRVISITRSAWCLG----ATLAGAITGLVAANFGWRIAVMVPGVIALLA 216 L+ E A RG+ + +S W +G A L + +++ + WR + + ALL Sbjct: 125 LMGEIVRAEHRGKAVGTVQSGWAIGWGAAALLYTLMFSVLSEEWAWRALFWIGVLPALLV 184 Query: 217 IYIRSTCPESPYWVRAQDRKRRISETLARGGTVSTEDSAWFGKAKSVGIRQVFMPDVLPA 276 +YIR PE +++++ ++ K + V +F P +L Sbjct: 185 LYIRKNVPEPALFLKSRHEQQ--------------------AKDRRVSSFAIFSPALLKT 224 Query: 277 TLVALFVACASTCIYGTVGAWMPLYLATEKHWS---TAEYSLFYVFYGLCGFLGLCLVGW 333 T++A + Y + W+P +L E++ S T Y + +F F+G + Sbjct: 225 TVLASLLCTGVQGGYYAITTWLPTFLKLERNLSVIGTGGYLMVIIF---GSFVGYICGAY 281 Query: 334 LIDKIGRRRTFIITLIEGAIFMTLWVYSE-----DRVLLWAFGLAWCLGFLGFWGPSTTL 388 L DK+GRR II + I T++ Y++ ++L+ F L + + F G L Sbjct: 282 LTDKLGRRANLIIFSLLSCI--TIFAYTQLTLTNTQMLVLGFPLGFSASGI-FSGMGAFL 338 Query: 389 TAEIFPTRIRGAANGVVWAIAYFVGFVLFPFVSIALQQHT------GSFALSFLCIPVLM 442 T E+FP+ +R G ++ VG LFP + L Q + G FA S CI ++M Sbjct: 339 T-ELFPSAVRATGQGFTYSFGRAVG-ALFPGLVGYLSQSSSLAFAIGVFAGSAYCIVLVM 396 Query: 443 IAMAIGVFLFVPEHTGKELN 462 L +PE K+L Sbjct: 397 S-------LLLPETKNKQLE 409 >STM0356 [GEPR] COG0477 Permeases of the major facilitator superfamily Sty Salmonella typhimurium LT2 Length = 417 Score = 114 bits (284), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 107/425 (25%), Positives = 183/425 (43%), Gaps = 36/425 (8%) Query: 44 TKTHWHIATANALGWGFDGMDGVIFALISPMVIKEFSLSLPEYRSGMQIALFVGIAGLYF 103 T T H+A A W D D I + + + F S+ + + + L V G Sbjct: 8 TTTQRHVAFACFTSWMLDAFDFFILVFVLSDLAEWFHASVSDVSIAIMLTLAVRPIGALL 67 Query: 104 WPWLADRYGRRTLLAVNIALFSLLMPVAAMSPTFAVFVIARSLLFFALNGEWSLGSMLVA 163 + +A++YGRR +L +NI F++ ++A SPTF F+I R + A+ G W + S L Sbjct: 68 FGRMAEKYGRRPILMLNILFFTVFELLSAWSPTFMAFLIFRVMYGVAMGGIWGVASSLAM 127 Query: 164 ETWPARLRGRVISITRSAWCLGATLAGAITGLVAANFGWRIAVMVPGVIALLAIYIRSTC 223 ET P R RG + I ++ + G A I GL + GWR ++ + +L YI Sbjct: 128 ETIPDRSRGLMSGIFQAGYPCGYLFASVIFGLFYSMVGWRGMFLIGALPVVLLPYIWFKV 187 Query: 224 PESPYWVRAQDRKRRISETLARGGTVSTEDSAWFGKAKSVGIRQVFMPDVLPATLVALFV 283 PESP W+ A+ RK E++A + V + L + Sbjct: 188 PESPVWLAARARK---------------ENTA---------LLPVLRKQWKLCLYLVLVM 223 Query: 284 ACASTCIYGTVGAWMPLYLATEKHWSTAEYSLFYVFYGLCGFLGLCLVGWLIDKIGRRRT 343 A + +GT + P +L + + S+ +FY + LG G L ++IGR++ Sbjct: 224 AFFNFFSHGTQDLY-PTFLKMQHGFDPHLISIIAIFYNIAAMLGGIFYGTLSERIGRKKA 282 Query: 344 FIITLIEGAIFMTLWVYSEDRVLLWAFGL-AWCLGFL--GFWGPSTTLTAEIFPTRIRGA 400 +I + LW +S + GL A+ + F+ G WG T E+ P R Sbjct: 283 IMIAAFLALPVLPLWAFSSGS---FTIGLGAFLMQFMVQGAWGVVPTWLNELVPANARAV 339 Query: 401 ANGVVWAIAYFVGFVLFPFVSIALQQHTGSFALSFLCIPVLMIAMAIGVFLFVPEHTGKE 460 G V+ + + V + + H G++ L+ + ++A A+ + + V G+E Sbjct: 340 LPGFVYQLGNLLASVNATLQARIAETHGGNYGLA-----MAIVAGAVAILICVFVAFGRE 394 Query: 461 LNEII 465 ++ Sbjct: 395 TRGVV 399 >PM0835 [GEPR] COG0477 Permeases of the major facilitator superfamily Pmu Pasteurella multocida Length = 405 Score = 112 bits (281), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 109/428 (25%), Positives = 191/428 (44%), Gaps = 29/428 (6%) Query: 40 TTEITKTHWHIATANALGWGFDGMDGVIFALISPMVIKEFSLSLPEYRSGMQIALFVGIA 99 + K W +A+G+ DG D +I + + + +L+ + S + L +A Sbjct: 2 NDNVNKDGWKALLGSAVGYAMDGFDLLILGFMLSAISADLALTPAQSGSLVTWTLIGAVA 61 Query: 100 GLYFWPWLADRYGRRTLLAVNIALFSLLMPVAAMSPTFAVFVIARSLLFFALNGEWSLGS 159 G + L+D+YGR +L I LF++ + A + + +I R++ L GE+ +G Sbjct: 62 GGIIFGALSDKYGRVRVLTWTIVLFAVFTGLCAFAQGYWDLLIYRTIAGIGLGGEFGIGM 121 Query: 160 MLVAETWPARLRGRVISITRSAWCLGATLAGAITGLVAANFGWRIAVMVPGVIALLAIYI 219 L AE WPAR R + S W +G A +T L+ + GWR +V A +A Y+ Sbjct: 122 ALAAEAWPARHRAKASSYVALGWQVGVLGAALLTPLLLPHIGWRGMFLVGIFPAFVAWYL 181 Query: 220 RSTCPESPYWVRAQDRKRRISETLARGGTVSTEDSAWFGKAKSVGIRQVFMPDVLPATLV 279 R+ E +V+ Q R K + ++ + D A + Sbjct: 182 RAKLHEPEIFVKKQTALER-------------------QKTSRLEPFRLLIKDKATAKIS 222 Query: 280 ALFVACASTCIYGTVG--AWMPLYLATEKHWSTAEYSLFYVFYGLCGFL-GLCLVGWLID 336 A V S +G G WMP +L+ + +S + S + +CG + G+ L G L D Sbjct: 223 AGIVVLTSVQNFGYYGIMIWMPNFLSKQLGFSLTK-SGVWTAVTICGMMFGIWLFGQLAD 281 Query: 337 KIGRRRTFIITLIEGAIFMTLWVYSE--DRVLLWAFGLAWCLGFLGFWGPSTTLTAEIFP 394 +IGR+ +F+ L + ++++ YS+ D + G + G G L +E +P Sbjct: 282 RIGRKPSFL--LFQFGAVISIFTYSQMSDPTYMLFAGAVLGMFVNGMMGGYGALMSEAYP 339 Query: 395 TRIRGAANGVVWAIAYFVGFVLFPFVSIALQQHTGSFALSFLCIPVLMIAMAIGVFLFVP 454 T+ R A V++ + VG V + ++ A++FL + +I M +FL +P Sbjct: 340 TQARATAQNVLFNLGRAVGGFGPIVVGALVSTYSFQLAIAFLA-SIYIIDMIATIFL-IP 397 Query: 455 EHTGKELN 462 E GK+L+ Sbjct: 398 ELKGKQLD 405