BLASTP 2.2.21 [Jun-14-2009]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0250.0016.p         (436 letters)

Database: COGmodified 
           192,987 sequences; 59,019,183 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SMb21000 [GEPR] COG0477 Permeases of the major facilitator super...    92   3e-18
mll3434 [J] COG2890 Methylase of polypeptide chain release facto...    70   8e-12
HI1559 [J] COG2890 Methylase of polypeptide chain release factor...    70   8e-12
ZhemK [J] COG2890 Methylase of polypeptide chain release factors...    70   1e-11
ECs1717 [J] COG2890 Methylase of polypeptide chain release facto...    70   1e-11

>SMb21000 [GEPR] COG0477 Permeases of the major facilitator superfamily Sme
           Sinorhizobium meliloti
          Length = 377

 Score = 92.0 bits (227), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 115/353 (32%), Positives = 167/353 (47%), Gaps = 41/353 (11%)

Query: 75  LSYSAAGFMNTINAAGYLAGALLA----SRLIER-FGLAASVRWSTLACALSLALCALSG 129
           LS + AG + + N  GYLAGA+LA    +   ER  GLAA V     A  L LA   L+ 
Sbjct: 25  LSPADAGLVASANFIGYLAGAVLAGYRWAHGRERGVGLAALV-----ATTLLLAAMGLTS 79

Query: 130 NFVVLSFARLLVGFAAAIG--FVGGGALAATIAQSRPERANFLLSLFYAGPGVGILASGL 187
           + +  S  R L G A+A    F+ G      + Q    R+  + S+ + G G GI  S +
Sbjct: 80  SVLAYSIIRFLAGLASAFAMIFISG----IVLGQGLAARSEHVPSVHFGGVGFGIALSSI 135

Query: 188 ---IAPFVLQGFGAGSW----WIVWWAMTLLSAIMIVPLMLAPLHADAAAGG-----IVR 235
              +AP  L G    S     W V   + L   +++  L+ A  HA  A GG     +VR
Sbjct: 136 AVWVAP--LAGVAGLSMSQADWFVGALVALAGTVLVAALLPASRHA--ANGGRPEVPLVR 191

Query: 236 TQFAVMPVLIYLAAYFLFGAGYIAYMTFMIAYVRDGGGGAAAQSAFWSLIGVSAFVTPWV 295
           ++    P++     Y  FG GY+   TF++A  RD  GG + +   W + GVSA ++ ++
Sbjct: 192 SR----PLMAMTLTYGFFGFGYVITATFLVAMARDASGGHSVEFLAWLITGVSAALSVYL 247

Query: 296 WGRVLALDRGGLATTIILG--VNALGASLPIFGHSPLLLAISALVFGVAFFAVVGSTTAF 353
           W    A+ R GLA    +G  V A+G  L +   SP    +  L+ G  F  V       
Sbjct: 248 W--RFAVPRFGLAGVYAIGLLVEAVGLVLTVSLPSPYAPLVGGLMLGATFMMVTAYGLQI 305

Query: 354 VRFNYPPQSWPTAIAAMTIAFGIGQTLGPIVVGAITDAVGSLSFALNVSAAML 406
            R    P+S   A+A MT AFGIGQ +GP+V G + +  GS +    V+A +L
Sbjct: 306 GR-QLAPESPRRALAFMTAAFGIGQIVGPLVAGWLAERTGSYALPTLVAAVVL 357


>mll3434 [J] COG2890 Methylase of polypeptide chain release factors Mlo
           Mesorhizobium loti
          Length = 290

 Score = 70.5 bits (171), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 41/74 (55%), Positives = 48/74 (64%), Gaps = 3/74 (4%)

Query: 2   RIGAVPDHQRDXILGIKEFWGLPLHLSAATLVPRPDTETVVERALELLRADGMAERALRI 61
           R G  P H+   ILG +EF+GL L LS  TL PRPDTET+VE  L  ++A    E   RI
Sbjct: 70  RAGGEPVHR---ILGYREFYGLRLSLSPETLEPRPDTETLVEAVLPFVKAMAAREGTCRI 126

Query: 62  ADLGTGSGAILLAL 75
            DLGTG+GAI LAL
Sbjct: 127 LDLGTGTGAIALAL 140


>HI1559 [J] COG2890 Methylase of polypeptide chain release factors Hin
           Haemophilus influenzae
          Length = 292

 Score = 70.5 bits (171), Expect = 8e-12,   Method: Compositional matrix adjust.
 Identities = 38/69 (55%), Positives = 49/69 (71%), Gaps = 3/69 (4%)

Query: 14  ILGIKEFWGLPLHLSAATLVPRPDTETVVERALELLRADGMAER--ALRIADLGTGSGAI 71
           ILG KEFW LPL++S  TL+PRPDTE +VE+AL++   + + E     RI DLGTG+GAI
Sbjct: 74  ILGEKEFWSLPLNVSKGTLIPRPDTEILVEKALQIA-LEKLEENPPHFRILDLGTGTGAI 132

Query: 72  LLALSYSAA 80
            LAL+   A
Sbjct: 133 ALALASELA 141


>ZhemK [J] COG2890 Methylase of polypeptide chain release factors EcZ
           Escherichia coli O157:H7 EDL933
          Length = 277

 Score = 69.7 bits (169), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 36/65 (55%), Positives = 46/65 (70%), Gaps = 5/65 (7%)

Query: 14  ILGIKEFWGLPLHLSAATLVPRPDTETVVERALELLRADGMAERALRIADLGTGSGAILL 73
           + G++EFW LPL +S ATL+PRPDTE +VE+AL  L      E+  RI DLGTG+GAI L
Sbjct: 71  LTGVREFWSLPLFVSPATLIPRPDTECLVEQALARL-----PEQPCRILDLGTGTGAIAL 125

Query: 74  ALSYS 78
           AL+  
Sbjct: 126 ALARE 130


>ECs1717 [J] COG2890 Methylase of polypeptide chain release factors Ecs
           Escherichia coli O157:H7
          Length = 277

 Score = 69.7 bits (169), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 36/65 (55%), Positives = 46/65 (70%), Gaps = 5/65 (7%)

Query: 14  ILGIKEFWGLPLHLSAATLVPRPDTETVVERALELLRADGMAERALRIADLGTGSGAILL 73
           + G++EFW LPL +S ATL+PRPDTE +VE+AL  L      E+  RI DLGTG+GAI L
Sbjct: 71  LTGVREFWSLPLFVSPATLIPRPDTECLVEQALARL-----PEQPCRILDLGTGTGAIAL 125

Query: 74  ALSYS 78
           AL+  
Sbjct: 126 ALARE 130