BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0246.0029.p (623 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming... 278 3e-74 AF0200 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 263 1e-69 HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 250 9e-66 ML0887 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 246 9e-65 Rv2187 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming)... 244 6e-64 >APE1307 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Ape Aeropyrum pernix Length = 651 Score = 278 bits (711), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 187/615 (30%), Positives = 301/615 (48%), Gaps = 17/615 (2%) Query: 10 AQLADVTIPQLLSRRRVELSDEIAFKQKRRGEWIATTWKQYSDEVAKLAATLQKAGFERG 69 ++ + T P LL R +AF+ KR G W TW+ Y VA A L+ G G Sbjct: 16 GEVYESTFPWLLEWRARTQPGRVAFRWKRYGIWRRYTWRDYYLRVAYAALALENLGLGPG 75 Query: 70 DRAAIMGDVSQEWLLADMAIICAGGIMVGVYFTSSAEEVGYYLGDSGTSFMFVGSELQLG 129 D A + W++ ++ + GG +G+Y S ++EVGY L F+ V + QL Sbjct: 76 DTGAFITFNRPSWVIYEVGVQLLGGRAMGIYRDSLSDEVGYLLDSGDAKFVLVEGQEQLD 135 Query: 130 TVLASGHADQLKKIIVLDPA----WKKAGGPANVVSLHDFW----SETSVDADILLQEQV 181 VL SG + KIIV + ++ G + +V+ D S D +++ + Sbjct: 136 RVLDSGV--DVDKIIVDEAKGLHQYRGMVG-SKLVTFGDVMRLGRKLYSDGGDQQVKKLM 192 Query: 182 AKAKASDLTSIGYTSGTTGFPKGAMLTHLSLLAGAHSVTLFTSKLRSDVHRVVVHLPMSH 241 + + + + TSGTTG PK AM++ ++LA AH + +R D V LP + Sbjct: 193 GELEPDMVCGLFTTSGTTGLPKLAMISFKNMLAMAHQLNT-VDPVREDWE-YVSFLPTAW 250 Query: 242 TVARAQATTLPLLIRLVPYFGENSAEFAQTVKEVKPTYFMAPPRFFQRYATQILSKVHSG 301 + + ++ F E + +E+ P + +PPR ++R A I+++V Sbjct: 251 IGEQMMSIPYHMIGGFKVNFPETPHTLWRDFREIAPHFMFSPPRVWERIAKDIMARVDDA 310 Query: 302 SEKQKQDYQLAMTIARKALDDRQAGGVPEP--FISGLFAVCQQQVFRPLLCEVGFEELTV 359 ++ ++LA I +A R G P L+ + R +L + G + + Sbjct: 311 DPIKRAAFRLAYKIGYEAARRRLVKGRSRPPTLWRALYYLAYWLALRAVLDKAGLKRIVR 370 Query: 360 TYTSSAAMPPELMSLWQLWGLQLKECYGQTELVGANLVQMAEWPEAGTVGVPLRDPAWET 419 YT A + + + + G+ LK+ YGQTE+ G +V + TVG PL P E Sbjct: 371 AYTGGAMIGEDYVIFYHSLGVNLKQIYGQTEVAGIAVVHPDDDVRPDTVGKPL--PLTEV 428 Query: 420 AVLEDGEMIVRGPGLFVGYWNKPEETKSALRDGWLYTGDIVDVDSTGLFRLVDRKKEIIN 479 + EDGE+++R P + GY+ E T + DGWL TGD+ + G +++DR KEII Sbjct: 429 KIAEDGEILMRSPAMMKGYYKNEEATAKTIVDGWLRTGDVGVLTEDGHLKVLDRAKEIII 488 Query: 480 TSNGKSISPTQIENELRRSPFISEAAVLGEGKKYLTALIEVNATATMDWARSHGLRIDRY 539 S+G ++P I+N+L+ SP+I EAA++G GK YL AL+ ++ WA + Y Sbjct: 489 LSDGTKVAPQVIQNKLKFSPYIGEAAIVGSGKPYLAALVNIDFEIVSRWAERRRIPFTSY 548 Query: 540 SELANSEIVVRMIEAEIGKANGRLARAEQIKAFRILPEELSPENGTMTPTRKKRRKQIME 599 S+L+ V+++++ EI K N RL E+I+ F L +E P++ MT TRK RR I Sbjct: 549 SDLSQKPEVLQLLKREIEKVNRRLPEKERIRRFVSLFKEFHPDDEEMTRTRKLRRMVIES 608 Query: 600 RYRSLIDTLYDESEE 614 RY LI+ +Y EE Sbjct: 609 RYAGLIEAIYSGDEE 623 >AF0200 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Afu Archaeoglobus fulgidus Length = 601 Score = 263 bits (671), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 186/584 (31%), Positives = 284/584 (48%), Gaps = 50/584 (8%) Query: 46 TWKQYSDEVAKLAATLQKAGFERGDRAAIMGDVSQEWLLADMAIICAGGIMVGVYFTSSA 105 T++++ + V KL+ L +G +GDR AI D EW +AD A++ AGG++V V+ + Sbjct: 46 TYEEFWERVRKLSKFLISSGLRKGDRVAIYADTRYEWEIADFAVLTAGGVVVTVHSVLNR 105 Query: 106 EEVGYYLGDSGTSFMFVGSELQLGTVLASGHADQLKKIIVLDPAWKKAGGPANVVSLHDF 165 E+V Y L DS + +F + + + + ++ L+ K G VS +F Sbjct: 106 EQVEYILRDSESRVVFTEKKY-------AENVPEDFEVYFLEELEKLVGE----VSDDEF 154 Query: 166 WSE-TSVDADILLQEQVAKAKASDLTSIGYTSGTTGFPKGAMLTHLSLLAGAHSVTLFTS 224 S SV+ D DL SI YTSGTTG PKGAMLTH + HSV T Sbjct: 155 ESRWKSVEPD-------------DLASIVYTSGTTGEPKGAMLTHWNWRFNCHSVMSITP 201 Query: 225 KLRSDVHRVVVHLPMSHTVARAQATTLPLLIRLVPYFGENSAEFAQTVKEVKPTYFMAPP 284 + H + +LP+SH R + R + +F +T VKP + P Sbjct: 202 FYPGEPH--ICYLPLSHVYQR--LVFFAGISRAATAVFCSPQQFLETSTAVKPVGLIVVP 257 Query: 285 RFFQRYATQILSKVHSGSEKQKQDYQLAMTIARKALDDRQAGGVPEPFISGLFAVCQQQV 344 R +R I+ KV K+ + + +A + G +++ + + V Sbjct: 258 RILERVNAGIVEKVEKSPALAKKIFYWSRGVAIECGKRMSRGEKYGFWLNIKRIIADRLV 317 Query: 345 FRPLLCEVGFEELTVTYTSSAAMPPELMSLWQLWGLQLKECYGQTELVGANLVQMAEWPE 404 F + +G + +S+A + EL ++ G+ + E YG TE + + + Sbjct: 318 FSKIRENLGLTRIRFVCSSAAELQKELAYMFNGMGIPVIEGYGMTETAAPSNLNPVGRFK 377 Query: 405 AGTVGVPLRDPAWETAVLEDGEMIVRGPGLFVGYWNKPEET-KSALRDGWLYTGDIVDVD 463 GTVG P+ P E A+ EDGE++VRG + GYW K ET K+ DGWL TGD+ + D Sbjct: 378 PGTVGPPI--PGIEEAIAEDGEILVRGDNVMKGYWRKEAETRKTFTEDGWLKTGDLGEFD 435 Query: 464 STGLFRLVDRKKEIINTSNGKSISPTQIENELRRSPFISEAAVLGEGKKYLTALIEVNAT 523 G + RKK II GK++SP IE EL ++P +S+A ++G+GK Y+T LI N + Sbjct: 436 EDGYLVFLGRKKHIIVLDTGKNVSPVPIEEELLKNPLVSDAVIIGDGKPYVTTLIVPNFS 495 Query: 524 ATMDWARSHGLRIDRYSELANSEI-----------------VVRMIEAEI-GKANGRLAR 565 +++A +G+ DR + I V+ + A+I N RLA+ Sbjct: 496 MLLEFADKNGIEYDRSRTIVQRSISGEEEIVAVDENLVENNAVKELYAKIVDDVNSRLAK 555 Query: 566 AEQIKAFRILPEELSPENGTMTPTRKKRRKQIMERYRSLIDTLY 609 E IK F+ILPE S E G +TPT KKRR I++RY I+ +Y Sbjct: 556 HETIKKFKILPEAFSLEKGEITPTLKKRRHVILKRYEKFIEEMY 599 >HI0002 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Hin Haemophilus influenzae Length = 607 Score = 250 bits (638), Expect = 9e-66, Method: Compositional matrix adjust. Identities = 174/590 (29%), Positives = 294/590 (49%), Gaps = 23/590 (3%) Query: 33 AFKQKRRGEWIATTWKQYSDEVAKLAATLQKAGFERGDRAAIMGDVSQEWLLADMAIICA 92 A + K G W +WK + +++ +L+ L + D+ AI + W + D+A + Sbjct: 31 ALRYKEHGLWRDISWKNFQEQLNQLSRALLAHNIDVQDKIAIFAHNMERWTIVDIATLQI 90 Query: 93 GGIMVGVYFTSSAEEVGYYLGDSGTSFMFVGSELQLGTVL-ASGHADQLKKIIVLDPAWK 151 I V +Y T++A++ + L + +FVG + Q L + H +L+KI+ + + Sbjct: 91 RAITVPIYATNTAQQAEFILNHADVKILFVGDQEQYDQTLEIAHHCPKLQKIVAMKSTIQ 150 Query: 152 KAGGPANVVSLHDFWSETSVDADILLQEQVAKAKASDLTSIGYTSGTTGFPKGAMLTHLS 211 P + + ++ D L Q ++ + + SDL +I YTSGTTG PKG ML + + Sbjct: 151 LQQDPLSCTWESFIKTGSNAQQDELTQ-RLNQKQLSDLFTIIYTSGTTGEPKGVMLDYAN 209 Query: 212 LLAGAHSVTLFTSKLR-SDVHRVVVHLPMSHTVARAQATTLPLLIRLVPYFGENSAEFAQ 270 L AH + L +D + LP SH RA A + ++ Y E++ + Sbjct: 210 L---AHQLETHDLSLNVTDQDISLSFLPFSHIFERAWAAYILHRGAILCYL-EDTNQVRS 265 Query: 271 TVKEVKPTYFMAPPRFFQRYATQILSKVHSGSEKQKQDYQLAMTIARKALDDRQAGGVPE 330 + E++PT A PRF+++ +L KV + ++ + A+++ +K D R A Sbjct: 266 ALTEIRPTLMCAVPRFYEKIYAAVLDKVQKAPKLRQIMFHWAISVGQKYFDLR-ANNKAI 324 Query: 331 PFI-SGLFAVCQQQVFRPLLCEVGFEELTVTYTSSAAMPPELMSLWQLWGLQLKECYGQT 389 PF+ FA+ + V L +G + + A + P + + G+ +K YG T Sbjct: 325 PFLLKKQFALADKLVLSKLRQLLG-GRIKMMPCGGAKLEPAIGLFFHAIGINIKLGYGMT 383 Query: 390 ELVGANLVQMAEWPE----AGTVGVPLRDPAWETAVLEDGEMIVRGPGLFVGYWNKPEET 445 E ++ W + ++G + P E + E+ E++VRG + GY+ KPEET Sbjct: 384 ETTAT----VSCWHDFQFNPNSIGTLM--PKAEVKIGENNEILVRGGMVMKGYYKKPEET 437 Query: 446 KSAL-RDGWLYTGDIVDVDSTGLFRLVDRKKEIINTSNGKSISPTQIENELRRSPFISEA 504 A DG+L TGD + D G + DR KE++ TSNGK I+P IE+++ + FI + Sbjct: 438 AQAFTEDGFLKTGDAGEFDEQGNLFITDRIKELMKTSNGKYIAPQYIESKIGKDKFIEQI 497 Query: 505 AVLGEGKKYLTALIEVNATATMDWARSHGLRI-DRYSELANSEIVVRMIEAEIGKANGRL 563 A++ + KKY++ALI + ++A+ ++ DR L NS+I+ +M E I L Sbjct: 498 AIIADAKKYVSALIVPCFDSLEEYAKQLNIKYHDRLELLKNSDIL-KMFEHRINAVQKEL 556 Query: 564 ARAEQIKAFRILPEELSPENGTMTPTRKKRRKQIMERYRSLIDTLYDESE 613 A EQ+K F +L + S + G +TPT K RRK I+ERYR I+ +Y E Sbjct: 557 AHFEQVKKFTLLSQAFSIKLGEITPTLKLRRKVILERYRKQIEAMYHSQE 606 >ML0887 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Mle Mycobacterium leprae Length = 600 Score = 246 bits (629), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 168/584 (28%), Positives = 291/584 (49%), Gaps = 21/584 (3%) Query: 32 IAFKQKRRGEWIATTWKQYSDEVAKLAATLQKAGFERGDRAAIMGDVSQEWLLADMAIIC 91 + ++++ G W T + + ++ A L G + GDR +I EW + D+AI+ Sbjct: 33 VIYQRQIDGVWTDVTCAEAASQIRSAALGLIALGMQAGDRVSIFSATCFEWAILDLAILS 92 Query: 92 AGGIMVGVYFTSSAEEVGYYLGDSGTSFMFVGSELQLGTV-LASGHADQLKKIIVLDPAW 150 G + V +Y TSSA++V + L DS +F ++ V +G L++++ ++ A Sbjct: 93 VGAVTVPIYETSSAKQVRWILQDSDAVLVFAETDAHATMVNQLAGELPALRQVLQINSAG 152 Query: 151 KKAGGPANVVSLHDFWSETSVDADIL-LQEQVAKAKASDLTSIGYTSGTTGFPKGAMLTH 209 A D +E D+ L ++ +A D ++ YTSGTTG PKG LTH Sbjct: 153 ATA---------LDQLAEAGASVDVAQLANRLKTLRAQDPATLIYTSGTTGRPKGCQLTH 203 Query: 210 LSLLAGAHSVTLFTSKLRSDVHRVVVHLPMSHTVARAQATTLPLLIRLVPYFGENSAEFA 269 +LL L R++V LP++H +ARA T ++ F + Sbjct: 204 SNLLYEVRGAKECLPTLLQPGQRLLVFLPLAHVLARA-LTLSAFTYKMTVGFTSDIKNLL 262 Query: 270 QTVKEVKPTYFMAPPRFFQR-YATQILSKVHSGSEKQKQDYQLAMTIARKALDDRQAGGV 328 + KPT ++ PR F++ Y T ++ ++ +E + + + +A A + + GG Sbjct: 263 PMLAVFKPTVVVSVPRVFEKVYNT---AEQNAANEGKSRIFAMAAQTAVEWSEASHRGG- 318 Query: 329 PEPFISGLFAVCQQQVFRPLLCEVGFEELTVTYTSSAAMPPELMSLWQLWGLQLKECYGQ 388 P + AV + V+R L +G + + + A + L ++ GL + E YG Sbjct: 319 PGLLLRARHAVFDRLVYRKLRAALG-GDCHASVSGGAPLGARLGHFYRGVGLTIHEGYGL 377 Query: 389 TELVGANLVQMAEWPEAGTVGVPLRDPAWETAVLEDGEMIVRGPGLFVGYWNKPEETKSA 448 TE A + A + GTVG L P + +DGE++VRG +F GYW+ + T A Sbjct: 378 TETSAAITLNQAGNVKVGTVGNLL--PGNSMRIADDGELLVRGNVVFSGYWHNEQGTIEA 435 Query: 449 LRDGWLYTGDIVDVDSTGLFRLVDRKKEIINTSNGKSISPTQIENELRRSPFISEAAVLG 508 +GW TGD+ +D G + RKKEII T+ GK+++P +E++LR P IS+A V+G Sbjct: 436 FTEGWFKTGDLGAIDEDGFLTITGRKKEIIVTAGGKNVAPALLEDQLRAHPLISQALVVG 495 Query: 509 EGKKYLTALIEVNATATMDWARSHGLRID-RYSELANSEIVVRMIEAEIGKANGRLARAE 567 + K ++ ALI ++ A W + + D +L +V ++A + +AN ++ AE Sbjct: 496 DAKPFVGALITIDPEAFNGWKQRNSKAADAAIGDLTTDPDLVAEVDAAVKQANLSVSHAE 555 Query: 568 QIKAFRILPEELSPENGTMTPTRKKRRKQIMERYRSLIDTLYDE 611 I+ FRILP + + + G +TPT K +R + +++ S I+ +Y++ Sbjct: 556 SIRKFRILPVDFTEQTGELTPTMKVKRNVVAQKFASDIEAIYEK 599 >Rv2187 [I] COG1022 Long-chain acyl-CoA synthetases (AMP-forming) Mtu Mycobacterium tuberculosis H37Rv Length = 600 Score = 244 bits (622), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 175/598 (29%), Positives = 293/598 (48%), Gaps = 45/598 (7%) Query: 30 DEIAFKQKRRGEWIATTWKQYSDEVAKLAATLQKAGFERGDRAAIMGDVSQEWLLADMAI 89 D + +++ G W T + ++++ A L G + GDR I EW + D AI Sbjct: 31 DYVIYQRLIDGVWTDVTCAEAANQIRAAALGLISLGVQAGDRVVIFSATRYEWAILDFAI 90 Query: 90 ICAGGIMVGVYFTSSAEEVGYYLGDSGTSFMFVGSELQLGTVLA--SGHADQLKKIIVLD 147 + G + V Y TSSAE+V + L DS +F ++ T++A SG L++++ + Sbjct: 91 LAVGAVTVPTYETSSAEQVRWVLQDSEAVVLFAETDSH-ATMVAELSGSVPALREVLQI- 148 Query: 148 PAWKKAGGPANVVSLHDFWSETSVDADIL-LQEQVAKAKASDLTSIGYTSGTTGFPKGAM 206 AG N + D +E D L ++A +++D ++ YTSGTTG PKG Sbjct: 149 -----AGSGPNAL---DRLTEAGASVDPAELTARLAALRSTDPATLIYTSGTTGRPKGCQ 200 Query: 207 LTHLSLLAGAHSVTLFTSKLRSDVHRVVVHLPMSHTVARAQATTLPLLIRLVPYFGENSA 266 LT +L+ + L R++V LP++H +ARA I + + + + Sbjct: 201 LTQSNLVHEIKGARAYHPTLLRKGERLLVFLPLAHVLARA--------ISMAAFHSKVTV 252 Query: 267 EFAQTVKEV-------KPTYFMAPPRFFQRYATQILSKVHSGSEKQKQDY---QLAMTIA 316 F +K + KPT ++ PR F+ KV++ +E+ + ++ A Sbjct: 253 GFTSDIKNLLPMLAVFKPTVVVSVPRVFE--------KVYNTAEQNAANAGKGRIFAIAA 304 Query: 317 RKALDDRQAG--GVPEPFISGLFAVCQQQVFRPLLCEVGFEELTVTYTSSAAMPPELMSL 374 + A+D +A G P + AV + V+R L +G + A + L Sbjct: 305 QTAVDWSEACDRGGPGLLLRAKHAVFDRLVYRKLRAALG-GNCRAAVSGGAPLGARLGHF 363 Query: 375 WQLWGLQLKECYGQTELVGANLVQMAEWPEAGTVGVPLRDPAWETAVLEDGEMIVRGPGL 434 ++ GL + E YG + G + + GTVG P+ P + +DGE++VRG + Sbjct: 364 YRGAGLTIYEGYGLSGTSGGVAISQFNDLKIGTVGKPV--PGNSLRIADDGELLVRGGVV 421 Query: 435 FVGYWNKPEETKSALRDGWLYTGDIVDVDSTGLFRLVDRKKEIINTSNGKSISPTQIENE 494 F GYW + T A DGW TGD+ VD G + RKKEII T+ GK+++P +E++ Sbjct: 422 FSGYWRNEQATTEAFTDGWFKTGDLGAVDEDGFLTITGRKKEIIVTAGGKNVAPAVLEDQ 481 Query: 495 LRRSPFISEAAVLGEGKKYLTALIEVNATATMDWA-RSHGLRIDRYSELANSEIVVRMIE 553 LR P IS+A V+G+ K ++ ALI ++ A W R+ +LA ++ I+ Sbjct: 482 LRAHPLISQAVVVGDAKPFIGALITIDPEAFEGWKQRNSKTAGASVGDLATDPDLIAEID 541 Query: 554 AEIGKANGRLARAEQIKAFRILPEELSPENGTMTPTRKKRRKQIMERYRSLIDTLYDE 611 A + +AN ++ AE I+ FRILP + + + G +TPT K +RK + E++ S I+ +Y++ Sbjct: 542 AAVKQANLAVSHAESIRKFRILPVDFTEDTGELTPTMKVKRKVVAEKFASDIEAIYNK 599