BLASTP 2.2.21 [Jun-14-2009]

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0243.0078.p         (196 letters)

Database: COGmodified 
           192,987 sequences; 59,019,183 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

MJ0256 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac...   109   5e-24
MA3298_2 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [...    87   2e-17
MTH1207 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [a...    86   6e-17
MK0393 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [ac...    80   3e-15

>MJ0256 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate
           synthase, pyruvate dehydrogenase (cytochrome),
           glyoxylate carboligase, phosphonopyruvate decarboxylase]
           Mja Methanococcus jannaschii
          Length = 188

 Score =  109 bits (272), Expect = 5e-24,   Method: Compositional matrix adjust.
 Identities = 55/130 (42%), Positives = 80/130 (61%), Gaps = 3/130 (2%)

Query: 26  VGGLGASTYDIAAAGDHDRNFYLWGGMGGAVMIGLGLALAQPTLPVVVITGDGEMLMGMG 85
           V  +G  + ++    D +RNFY+ G MG A  IGLGLAL      V+VI GDG +LM +G
Sbjct: 23  VSNIGIPSKELYYVKDRERNFYMLGSMGLASSIGLGLALNCED-KVIVIDGDGSILMNLG 81

Query: 86  SLATVGLQQPKNLSIIVLDNEAYGETGGQASHTGGTADLVGVAKACGIGDSRAISTMAEV 145
           SL+T+G   PKN  ++++DN AYG TG Q +HTG   +L  +AK CG+ D+    ++ E 
Sbjct: 82  SLSTIGYMNPKNYILVIIDNSAYGSTGNQKTHTGKNTNLEEIAKGCGL-DTITTESLEEF 140

Query: 146 EA-FASSLQD 154
           E  F ++L +
Sbjct: 141 EKEFKNALNE 150


>MA3298_2 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate
           synthase, pyruvate dehydrogenase (cytochrome),
           glyoxylate carboligase, phosphonopyruvate decarboxylase]
           Mac Methanosarcina acetivorans str.C2A
          Length = 186

 Score = 87.0 bits (214), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 52/141 (36%), Positives = 71/141 (50%), Gaps = 2/141 (1%)

Query: 9   RRAVVAELLKDRKGAFAVGGLGASTYDIAAAGDHDRNFYLWGGMGGAVMIGLGLALAQPT 68
           R   +  L  +      V  LG    ++ A  D + NFY++G MG    IGLGLAL    
Sbjct: 1   RNDAICTLASELDDEITVTNLGVPCKELYACRDRELNFYMFGSMGLVSSIGLGLALRSEK 60

Query: 69  LPVVVITGDGEMLMGMGSLATVGLQQPKNLSIIVLDNEAYGETGGQASHTGGTADLVGVA 128
             V+   GDG +LM   +L  +  + PKNL II LDN AYG TG Q +      DL   A
Sbjct: 61  T-VITFDGDGSLLMNPNALLEIAKEAPKNLIIIALDNGAYGSTGSQETCALRYIDLEIFA 119

Query: 129 KACGIGDSRAISTMA-EVEAF 148
            ACGI ++  +++    +EAF
Sbjct: 120 NACGIQNTAKVNSKEGVIEAF 140


>MTH1207 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate
           synthase, pyruvate dehydrogenase (cytochrome),
           glyoxylate carboligase, phosphonopyruvate decarboxylase]
           Mth Methanothermobacter thermautotrophicus
          Length = 185

 Score = 85.5 bits (210), Expect = 6e-17,   Method: Compositional matrix adjust.
 Identities = 58/182 (31%), Positives = 87/182 (47%), Gaps = 8/182 (4%)

Query: 6   LLDRRAVVAELLKDRKGAFAVGGLGASTYDIAAAGDHDRNFYLWGGMGGAVMIGLGLALA 65
           +L+R   +  +    +    +  LG  + ++ +  D  R+FY+ G MG A  IGLGLAL+
Sbjct: 2   MLERIEAIERITGVLEDELVICNLGFPSRELYSIRDSPRHFYMLGSMGMASSIGLGLALS 61

Query: 66  QPTLPVVVITGDGEMLMGMGSLATVGLQQPKNLSIIVLDNEAYGETGGQASHTGGTADLV 125
           Q    VVV+ GDG +LM +G L T   Q P NL I++LDN  Y  TG Q ++     DL 
Sbjct: 62  QER-RVVVLDGDGSILMNLGGLVTAAAQSPGNLIIVLLDNRCYATTGSQCTY-ADVIDLG 119

Query: 126 GVAKACGIGDSRAISTMAEVEAFASSLQDVTAGPRFASAKIDGANLERVLSSRDGTYLVN 185
            VA++ G    R    +   +A A        GP FA   +   N +  +   D   ++ 
Sbjct: 120 AVAESMGFNVIRFADDLNFEQALAMD------GPVFAHVPVKPGNADVPVIDLDAEEIIE 173

Query: 186 RI 187
           R 
Sbjct: 174 RF 175


>MK0393 [EH] COG0028 Thiamine pyrophosphate-requiring enzymes [acetolactate
           synthase, pyruvate dehydrogenase (cytochrome),
           glyoxylate carboligase, phosphonopyruvate decarboxylase]
           Mka Methanopyrus kandleri AV19
          Length = 188

 Score = 79.7 bits (195), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 53/161 (32%), Positives = 84/161 (52%), Gaps = 9/161 (5%)

Query: 12  VVAELLKDRKGAFAVGGLGASTYDIAAAGDHDRNFYLWGGMGGAVMIGLGLALAQPTLPV 71
           VVA++  +R  A     LG    ++    D   NFY+ G MG +  +GLGLAL      V
Sbjct: 11  VVADV-AERYDAVVTVHLGFPARELYRVNDRRLNFYMLGAMGQSCSVGLGLALCT-DREV 68

Query: 72  VVITGDGEMLMGMGSLATVGLQQPKNLSIIVLDNEAYGETGGQASHTGGTADLVGVAKAC 131
           + I GDG ++M MG L T+  ++P+N +++++DN  Y  TG Q + +    D   VA+A 
Sbjct: 69  LAIEGDGGLMMNMGVLPTIAQERPRNYTLVLIDNSTYATTGDQPTPSDRI-DWEKVAEAH 127

Query: 132 GIGDSRAISTMAEVEAFASSLQD--VTAGPRFASAKIDGAN 170
           G+    A    +E E+   +L+D   T GPR    +++  N
Sbjct: 128 GLTYFEA----SEPESAEVALEDALATEGPRMVRLEVEPGN 164