BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0242.0001.p (441 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0703 [J] COG0621 2-methylthioadenine synthetase Bme Brucell... 678 0.0 mlr0499 [J] COG0621 2-methylthioadenine synthetase Mlo Mesorhizo... 673 0.0 PA0916 [J] COG0621 2-methylthioadenine synthetase Pae Pseudomona... 596 e-170 STM0852 [J] COG0621 2-methylthioadenine synthetase Sty Salmonell... 583 e-166 yliG [J] COG0621 2-methylthioadenine synthetase Eco Escherichia ... 583 e-166 >BMEII0703 [J] COG0621 2-methylthioadenine synthetase Bme Brucella melitensis Length = 437 Score = 678 bits (1749), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/434 (73%), Positives = 371/434 (85%), Gaps = 1/434 (0%) Query: 5 AAPKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADIVIVNTCGFLDSAKQESLA 64 +AP+VSFVSLGCPKALVDSERIIT LR+EGYE++RKHDGAD+VIVNTCGFLDSA+ ESL Sbjct: 2 SAPRVSFVSLGCPKALVDSERIITGLRSEGYEISRKHDGADLVIVNTCGFLDSARDESLE 61 Query: 65 AIGEAMAENGKVIVTGCMGAEPEQIESAYPGVLSITGPQQYESVLDAVHRALPPAHNPHI 124 AIG A+ ENGKVIVTGC+GAEP+ I +P VL+ITGPQ YESV++AVH PPAH+P + Sbjct: 62 AIGLALNENGKVIVTGCLGAEPDVIRERHPNVLAITGPQAYESVMNAVHEVAPPAHDPFV 121 Query: 125 DLVPPQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPANDVLREAEKLVKAGV 184 DLVPPQGVKLTPRHYAYLKISEGC+NRCSFCIIP LRGDLVSRP N+VLREAEKLV+AGV Sbjct: 122 DLVPPQGVKLTPRHYAYLKISEGCSNRCSFCIIPALRGDLVSRPINEVLREAEKLVQAGV 181 Query: 185 KELLVISQDTSAYGVDLKYAESPWQDRGVRAKFFDLAKELGELGAWVRLQYVYPYPHVDE 244 KE+LVISQDTSAYG+D+KY E+ WQDR VR KF DL++ELGE+G WVR+ YVYPYPHVDE Sbjct: 182 KEILVISQDTSAYGLDIKYQEAMWQDRTVRTKFLDLSRELGEMGVWVRMHYVYPYPHVDE 241 Query: 245 VIGLMTEGKVLPYLDIPFQHASPDVLRAMKRPAAQEKTLARIQKWREQCPELTLRSTFIV 304 VI LM EGK+LPYLDIPFQHASP VL+ M+RPA QEKT RIQ WRE CP+L +RSTFIV Sbjct: 242 VIPLMAEGKILPYLDIPFQHASPAVLKNMRRPAHQEKTSRRIQAWRETCPDLAVRSTFIV 301 Query: 305 GFPGETDSDFAYLLDWLDEAGIDRLGCFKYEPVAGATSNAIG-NAVPDEVKQERWNALMA 363 G+PGET+ DF LLDWLDEA I+R GCFKYE V GA +N +G VP+EVK+ RW+ MA Sbjct: 302 GYPGETEEDFQMLLDWLDEAKIERAGCFKYEAVKGAKANDLGLEQVPEEVKEARWHRFMA 361 Query: 364 RQQKISARRLKRKVGTRQQVIIDEVGPTVAKGRSKADAPEIDGAVYLTSRRPLKIGEIVT 423 +QQ+IS LK+KVG R VIIDE T+ KGR++ DAPEIDG+V+++SRRPL++G+IVT Sbjct: 362 KQQQISTNLLKKKVGKRLPVIIDEANGTIGKGRTRYDAPEIDGSVHISSRRPLRVGDIVT 421 Query: 424 AKIERSDQYDLHGS 437 KIE SD YDLHG+ Sbjct: 422 VKIEASDAYDLHGT 435 >mlr0499 [J] COG0621 2-methylthioadenine synthetase Mlo Mesorhizobium loti Length = 437 Score = 673 bits (1737), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/434 (73%), Positives = 370/434 (85%), Gaps = 1/434 (0%) Query: 5 AAPKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADIVIVNTCGFLDSAKQESLA 64 +AP+VSFVSLGCPKALVDSERIITRLRAEGYE+ARKHDGAD+V+VNTCGFLDSA+ ESL Sbjct: 2 SAPRVSFVSLGCPKALVDSERIITRLRAEGYEIARKHDGADLVVVNTCGFLDSARDESLN 61 Query: 65 AIGEAMAENGKVIVTGCMGAEPEQIESAYPGVLSITGPQQYESVLDAVHRALPPAHNPHI 124 AIG A++ENG+VIVTGC+GAEP+ I +P VL+ITGPQ YESV+ AVH A PP+H+P+I Sbjct: 62 AIGSALSENGRVIVTGCLGAEPDVIREKHPNVLAITGPQAYESVMAAVHEAAPPSHDPYI 121 Query: 125 DLVPPQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPANDVLREAEKLVKAGV 184 DL+PPQGVKLTPRHYAYLKISEGCNNRC+FCIIP LRGDLVSRPA DVLREAEKL KAGV Sbjct: 122 DLLPPQGVKLTPRHYAYLKISEGCNNRCTFCIIPALRGDLVSRPAADVLREAEKLAKAGV 181 Query: 185 KELLVISQDTSAYGVDLKYAESPWQDRGVRAKFFDLAKELGELGAWVRLQYVYPYPHVDE 244 KELLVISQDTSAYG+D+KY S + DR VRAKF DL++ELG+LG WVR+ YVYPYPHV + Sbjct: 182 KELLVISQDTSAYGIDIKYQTSMFGDREVRAKFLDLSEELGKLGIWVRMHYVYPYPHVAD 241 Query: 245 VIGLMTEGKVLPYLDIPFQHASPDVLRAMKRPAAQEKTLARIQKWREQCPELTLRSTFIV 304 VI LM EGK+LPYLDIPFQHASP VL+ M+RPA EKTL RI+ WR+ CP+L +RSTFIV Sbjct: 242 VIPLMAEGKILPYLDIPFQHASPQVLKNMRRPAHGEKTLERIRGWRDVCPDLAIRSTFIV 301 Query: 305 GFPGETDSDFAYLLDWLDEAGIDRLGCFKYEPVAGATSNAIG-NAVPDEVKQERWNALMA 363 GFPGETD DF LLDWLDEA IDR GCFKYEPV GA SN +G VP +VK+ RW+ M Sbjct: 302 GFPGETDEDFEMLLDWLDEAKIDRAGCFKYEPVKGARSNDLGLEQVPQDVKEARWHRFMQ 361 Query: 364 RQQKISARRLKRKVGTRQQVIIDEVGPTVAKGRSKADAPEIDGAVYLTSRRPLKIGEIVT 423 RQQKISA +L +KVG R V+IDE T AKGR+K DAPEIDG+V++ SRRP++ G+IVT Sbjct: 362 RQQKISATQLAKKVGKRLPVLIDEAHGTSAKGRTKYDAPEIDGSVHIQSRRPMRAGDIVT 421 Query: 424 AKIERSDQYDLHGS 437 KIER+D YDL+GS Sbjct: 422 VKIERADAYDLYGS 435 >PA0916 [J] COG0621 2-methylthioadenine synthetase Pae Pseudomonas aeruginosa Length = 440 Score = 596 bits (1536), Expect = e-170, Method: Compositional matrix adjust. Identities = 291/432 (67%), Positives = 340/432 (78%), Gaps = 4/432 (0%) Query: 6 APKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADIVIVNTCGFLDSAKQESLAA 65 PKV FVSLGCPKALVDSERI+T+LR EGYE+ ++ AD+V+VNTCGF+DSAK ESL Sbjct: 5 TPKVGFVSLGCPKALVDSERILTQLRMEGYEVVPTYEDADVVVVNTCGFIDSAKAESLEV 64 Query: 66 IGEAMAENGKVIVTGCMGAEPEQIESAYPGVLSITGPQQYESVLDAVHRALPPA--HNPH 123 IGEA+AENGKVIVTGCMG E I +P VL++TGPQQYE V+ AVH +PP HNP Sbjct: 65 IGEAIAENGKVIVTGCMGVEEHAIRDVHPSVLAVTGPQQYEQVVTAVHEVVPPKTEHNPL 124 Query: 124 IDLVPPQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPANDVLREAEKLVKAG 183 +DLVPPQGVKLTPRHYAYLKISEGCN+ CSFCIIP +RG LVSRP DVL EAE+LVKAG Sbjct: 125 VDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLSEAERLVKAG 184 Query: 184 VKELLVISQDTSAYGVDLKYAESPWQDRGVRAKFFDLAKELGELGAWVRLQYVYPYPHVD 243 VKELLVISQDTSAYGVDLKY W + V+ + +L + L +G WVRL YVYPYP+VD Sbjct: 185 VKELLVISQDTSAYGVDLKYKTDFWNGQPVKTRMKELCEALSSMGVWVRLHYVYPYPNVD 244 Query: 244 EVIGLMTEGKVLPYLDIPFQHASPDVLRAMKRPAAQEKTLARIQKWREQCPELTLRSTFI 303 +VI LM GK+LPYLDIPFQHASP VL+AMKRPA ++KTLARI++WRE CPELT+RSTFI Sbjct: 245 DVIPLMAAGKLLPYLDIPFQHASPKVLKAMKRPAFEDKTLARIKQWREICPELTIRSTFI 304 Query: 304 VGFPGETDSDFAYLLDWLDEAGIDRLGCFKYEPVAGATSNAIG-NAVPDEVKQERWNALM 362 VGFPGET+ DF YLLDWL EA +DR+GCF+Y PV GA +N +G VPDEVKQ+RW M Sbjct: 305 VGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANELGLEPVPDEVKQDRWERFM 364 Query: 363 ARQQKISARRLKRKVGTRQQVIIDEVGPTVAKGRSKADAPEIDGAVYLTSRRPLKIGEIV 422 A QQ ISA RL+ KVG +V+IDEV A GRS ADAPEIDG V++ S LK G+ V Sbjct: 365 AHQQAISAARLQLKVGKEIEVLIDEVDEQGAVGRSWADAPEIDGNVFVDSDE-LKPGDKV 423 Query: 423 TAKIERSDQYDL 434 +I +D+YDL Sbjct: 424 RVRITDADEYDL 435 >STM0852 [J] COG0621 2-methylthioadenine synthetase Sty Salmonella typhimurium LT2 Length = 441 Score = 583 bits (1503), Expect = e-166, Method: Compositional matrix adjust. Identities = 281/432 (65%), Positives = 334/432 (77%), Gaps = 1/432 (0%) Query: 7 PKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADIVIVNTCGFLDSAKQESLAAI 66 PK+ FVSLGCPK LVDSERI+T LR EGY++ ++D AD+VIVNTCGF+DSA QESL AI Sbjct: 8 PKIGFVSLGCPKNLVDSERILTELRTEGYDVVPRYDDADMVIVNTCGFIDSAVQESLEAI 67 Query: 67 GEAMAENGKVIVTGCMGAEPEQIESAYPGVLSITGPQQYESVLDAVHRALP-PAHNPHID 125 GEA+ ENGKVIVTGC+GA+ +QI +P VL ITGP YE VL VH +P P HNP + Sbjct: 68 GEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLQHVHHYVPKPKHNPFLS 127 Query: 126 LVPPQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPANDVLREAEKLVKAGVK 185 LVP QGVKLTPRHYAYLKISEGCN+RC+FCIIP +RGDLVSRP DVL EA++LV AGVK Sbjct: 128 LVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLSEAKRLVDAGVK 187 Query: 186 ELLVISQDTSAYGVDLKYAESPWQDRGVRAKFFDLAKELGELGAWVRLQYVYPYPHVDEV 245 E+LVISQDTSAYGVD+K+ V+ L ++L +LG W RL YVYPYPHVD+V Sbjct: 188 EILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGVWTRLHYVYPYPHVDDV 247 Query: 246 IGLMTEGKVLPYLDIPFQHASPDVLRAMKRPAAQEKTLARIQKWREQCPELTLRSTFIVG 305 I LM EGK+LPYLDIP QHASP +L+ MKRP + ++ LARI++WRE CPELTLRSTFIVG Sbjct: 248 IPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVG 307 Query: 306 FPGETDSDFAYLLDWLDEAGIDRLGCFKYEPVAGATSNAIGNAVPDEVKQERWNALMARQ 365 FPGET+ DF LLD+L EA +DR+GCFKY PV GA +N + + VP+EVK+ERWN M Q Sbjct: 308 FPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGAGANELPDQVPEEVKEERWNRFMQLQ 367 Query: 366 QKISARRLKRKVGTRQQVIIDEVGPTVAKGRSKADAPEIDGAVYLTSRRPLKIGEIVTAK 425 Q+ISA RL+ KVG VI+DEV A GRS ADAPEIDGAVYL +K G+IV K Sbjct: 368 QQISAERLQEKVGREILVIVDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKPGDIVRVK 427 Query: 426 IERSDQYDLHGS 437 +E +D+YDL GS Sbjct: 428 VENADEYDLWGS 439 >yliG [J] COG0621 2-methylthioadenine synthetase Eco Escherichia coli K12 Length = 441 Score = 583 bits (1502), Expect = e-166, Method: Compositional matrix adjust. Identities = 281/432 (65%), Positives = 335/432 (77%), Gaps = 1/432 (0%) Query: 7 PKVSFVSLGCPKALVDSERIITRLRAEGYELARKHDGADIVIVNTCGFLDSAKQESLAAI 66 PK+ FVSLGCPK LVDSERI+T LR EGY++ +D AD+VIVNTCGF+DSA QESL AI Sbjct: 8 PKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAI 67 Query: 67 GEAMAENGKVIVTGCMGAEPEQIESAYPGVLSITGPQQYESVLDAVHRALP-PAHNPHID 125 GEA+ ENGKVIVTGC+GA+ +QI +P VL ITGP YE VL+ VH +P P HNP + Sbjct: 68 GEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLS 127 Query: 126 LVPPQGVKLTPRHYAYLKISEGCNNRCSFCIIPKLRGDLVSRPANDVLREAEKLVKAGVK 185 LVP QGVKLTPRHYAYLKISEGCN+RC+FCIIP +RGDLVSRP +VL EA++LV AGVK Sbjct: 128 LVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGEVLSEAKRLVDAGVK 187 Query: 186 ELLVISQDTSAYGVDLKYAESPWQDRGVRAKFFDLAKELGELGAWVRLQYVYPYPHVDEV 245 E+LVISQDTSAYGVD+K+ V+ L ++L +LG W RL YVYPYPHVD+V Sbjct: 188 EILVISQDTSAYGVDVKHRTGFHNGEPVKTSMVSLCEQLSKLGIWTRLHYVYPYPHVDDV 247 Query: 246 IGLMTEGKVLPYLDIPFQHASPDVLRAMKRPAAQEKTLARIQKWREQCPELTLRSTFIVG 305 I LM EGK+LPYLDIP QHASP +L+ MKRP + ++ LARI++WRE CPELTLRSTFIVG Sbjct: 248 IPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVG 307 Query: 306 FPGETDSDFAYLLDWLDEAGIDRLGCFKYEPVAGATSNAIGNAVPDEVKQERWNALMARQ 365 FPGET+ DF LLD+L EA +DR+GCFKY PV GA +NA+ + VP+EVK+ERWN M Q Sbjct: 308 FPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQ 367 Query: 366 QKISARRLKRKVGTRQQVIIDEVGPTVAKGRSKADAPEIDGAVYLTSRRPLKIGEIVTAK 425 Q+ISA RL+ KVG VIIDEV A GRS ADAPEIDGAVYL +K G+I+ K Sbjct: 368 QQISAERLQEKVGREILVIIDEVDEEGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVK 427 Query: 426 IERSDQYDLHGS 437 +E +D+YDL GS Sbjct: 428 VEHADEYDLWGS 439