BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0241.0016.p (230 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr5068 [R] COG1451 Predicted metal-dependent hydrolase Mlo Meso... 188 1e-47 BMEI2015 [R] COG1451 Predicted metal-dependent hydrolase Bme Bru... 175 9e-44 AGc25 [R] COG1451 Predicted metal-dependent hydrolase Atu Agroba... 171 1e-42 SMc02768 [R] COG1451 Predicted metal-dependent hydrolase Sme Sin... 164 1e-40 CC0251 [R] COG1451 Predicted metal-dependent hydrolase Ccr Caulo... 119 4e-27 >mlr5068 [R] COG1451 Predicted metal-dependent hydrolase Mlo Mesorhizobium loti Length = 252 Score = 188 bits (477), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 96/211 (45%), Positives = 131/211 (62%), Gaps = 5/211 (2%) Query: 18 RARRYTLRIHPTDREAILTMPPRGTIPEAKEFANLHGGWIAARLGRLPKAAPFQPGTVVP 77 RARR TLRI + +T+PP E F + H W+ RL ++P +PG +P Sbjct: 38 RARRLTLRIDSGGQGLRITVPPGLRRGEVDRFLDRHQDWLEQRLAKVPTRPQVRPGIKIP 97 Query: 78 LRGVPHRLVHRSGERGTVWTETRDSGEKIICVAGGVEHMDRRVHDFLKREARKDLQKAAQ 137 +RGVPHR+VH +RGTV T +RD ++ V G H+ RR+ DFLKREA+K+++K Sbjct: 98 IRGVPHRIVHEPSKRGTV-TVSRDERGPLLIVHGDRIHLPRRIADFLKREAKKEIEKLVI 156 Query: 138 LHAAELGVRVR----RXQSSRWGSCTSAGSLSFSWRLILAPPFVLDYLAAHEVAHLVEMN 193 H LG R + + SSRWGSCTS G+LSFSWR+++AP V++YL AHEVAHL EMN Sbjct: 157 KHTEALGKRAKAIRYKDTSSRWGSCTSEGNLSFSWRIMMAPQPVINYLVAHEVAHLKEMN 216 Query: 194 HSARFWRVVDKVCASVTRAKNWLDTYGNDLH 224 H +FW++ +K+C R K+WL G L Sbjct: 217 HGPKFWKLCEKLCPDTDRCKDWLKRNGGALQ 247 >BMEI2015 [R] COG1451 Predicted metal-dependent hydrolase Bme Brucella melitensis Length = 253 Score = 175 bits (443), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 92/220 (41%), Positives = 130/220 (59%), Gaps = 9/220 (4%) Query: 9 FTVRLRRHRRARRYTLRIHPTDREAILTMPPRGTIPEAKEFANLHGGWIAARLGRLPKAA 68 +R+ + RA+R TLRI P + +T+PP E + F N H GWI +R+ +LP Sbjct: 34 LPLRVYENPRAKRLTLRIEPGGKGLRITVPPGLPEREVQNFLNRHEGWIESRIAKLPDQP 93 Query: 69 PFQPGTVVPLRGVPHRLVHRSGERGTVWTETRDSGEKIICVAGGVEHMDRRVHDFLKREA 128 + G +P+RGV H +VH+ G RGTV + I+ V G H+ RRV D+LKRE Sbjct: 94 GVRAGIKIPIRGVAHLIVHQPG-RGTVECIEGN----ILLVHGDPSHLPRRVADYLKREV 148 Query: 129 RKDLQKAAQLHAAELGVRVR----RXQSSRWGSCTSAGSLSFSWRLILAPPFVLDYLAAH 184 R+D++ H A +G + + + SRWGSCTS G LSFSWR+ +APP V++YL AH Sbjct: 149 RRDIEALVVRHTAAVGRKAKAVRFKDTKSRWGSCTSDGVLSFSWRIGMAPPPVINYLVAH 208 Query: 185 EVAHLVEMNHSARFWRVVDKVCASVTRAKNWLDTYGNDLH 224 E AHL+EMNH +FW++ ++C R K WL G+ L Sbjct: 209 EAAHLIEMNHGPKFWKLCQELCPDTERCKAWLKHNGSALQ 248 >AGc25 [R] COG1451 Predicted metal-dependent hydrolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 255 Score = 171 bits (434), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 87/218 (39%), Positives = 126/218 (57%), Gaps = 4/218 (1%) Query: 11 VRLRRHRRARRYTLRIHPTDREAILTMPPRGTIPEAKEFANLHGGWIAARLGRLPKAAPF 70 + +R + RA R TLRI P R LT+P + +F H GW+ +L + Sbjct: 33 ITVRENPRATRITLRIEPGGRALKLTIPMGLHHRQVDDFLERHQGWLEGKLLKFSPDDGL 92 Query: 71 QPGTVVPLRGVPHRLVHRSGERGTVWTETRDSGEKIICVAGGVEHMDRRVHDFLKREARK 130 +PG + +RG+ HR+ H RG G ++ V+G EH+ RR+ FLK+EA+ Sbjct: 93 RPGATIDIRGISHRIEHTGSLRGLTHIAKDADGVAVLKVSGAPEHLRRRIATFLKKEAKA 152 Query: 131 DLQKAAQLHAAELGVRVR----RXQSSRWGSCTSAGSLSFSWRLILAPPFVLDYLAAHEV 186 DL++ +H G VR + SRWGSC+ G+LSFSWR+++AP V+DYLAAHEV Sbjct: 153 DLERLVAIHGRAAGRPVRSISMKDTRSRWGSCSHDGNLSFSWRIVMAPDRVIDYLAAHEV 212 Query: 187 AHLVEMNHSARFWRVVDKVCASVTRAKNWLDTYGNDLH 224 AHL EMNH +FW + +K+C + AK+WL +G+ LH Sbjct: 213 AHLSEMNHGPKFWALCEKLCPHTSEAKDWLKRHGSKLH 250 >SMc02768 [R] COG1451 Predicted metal-dependent hydrolase Sme Sinorhizobium meliloti Length = 251 Score = 164 bits (416), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 89/222 (40%), Positives = 123/222 (55%), Gaps = 5/222 (2%) Query: 7 QFFTVRLRRHRRARRYTLRIHPTDREAILTMPPRGTIPEAKEFANLHGGWIAARLGRLPK 66 + + +R++ RA R TLRI P R LT+P E F H GW+ +L R Sbjct: 26 KVLPLTIRQNARATRMTLRIEPGGRALKLTVPEGLPEREVSAFLIRHQGWLMTKLARFSG 85 Query: 67 AAPFQPGTVVPLRGVPHRLVHRSGERGTVWTETRDSGEKIICVAGGVEHMDRRVHDFLKR 126 + + G + +RGV HR+ RG D E ++ V G EH+ RR+ D+LK+ Sbjct: 86 ESELEDGGTILVRGVAHRIERTGRLRGLTEALVIDD-EAVLRVGGAEEHLRRRIADYLKK 144 Query: 127 EARKDLQKAAQLHAAELGVRVR----RXQSSRWGSCTSAGSLSFSWRLILAPPFVLDYLA 182 EAR +L++ ++A G R R + SRWGSC++ G LSFSWR+ +APP V+ YLA Sbjct: 145 EARSELERLVAVYAGRTGRRARSLSLKDTRSRWGSCSAEGDLSFSWRIAMAPPKVIAYLA 204 Query: 183 AHEVAHLVEMNHSARFWRVVDKVCASVTRAKNWLDTYGNDLH 224 AHEVAHL EMNH RFW + + +C AK+WL G LH Sbjct: 205 AHEVAHLQEMNHGPRFWALCESLCPETKDAKHWLKRNGTMLH 246 >CC0251 [R] COG1451 Predicted metal-dependent hydrolase Ccr Caulobacter vibrioides Length = 239 Score = 119 bits (299), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 84/228 (36%), Positives = 112/228 (49%), Gaps = 18/228 (7%) Query: 9 FTVRLRRHRRARRYTLRIHPTDREAILTMPPRGTIPEAKEFANLHGGWIAARLGRLPKAA 68 + VRL+ RARR +LR+ E + P + EA FA GWIA +L LP+ Sbjct: 20 WPVRLKVDGRARRISLRVDRARSEIVALSPTPRRLNEAVAFAQEKAGWIAKQLAALPERM 79 Query: 69 PFQPGTVVPLRGVPHRLVHRSGERGTVWTETRDSGEKIICVAGGVEHMDRRVHDFLKREA 128 PG V+ L P RL +G R G++I VA + +V KREA Sbjct: 80 SLAPGGVLRLNDKPFRLEVGAGR-------ARIDGDRI--VAPDDANWGLKVIRLAKREA 130 Query: 129 RKDLQKAAQLHAAELGVRVRRXQ----SSRWGSCTSAGS-----LSFSWRLILAPPFVLD 179 L + +HAA LG V +RWGSC A S + +SWRLILAP V D Sbjct: 131 LTVLTERTAIHAAALGRPVPSVAIADPKARWGSCRPATSRAPAAIRYSWRLILAPAVVAD 190 Query: 180 YLAAHEVAHLVEMNHSARFWRVVDKVCASVTRAKNWLDTYGNDLHRYG 227 Y+AAHE AHL+E NH RFW + +++ T + WL + +LH G Sbjct: 191 YVAAHECAHLIEANHGPRFWALCEQLIGDPTPHRAWLRAHAAELHAIG 238