BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0020.p (432 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC0614 Non COG 353 5e-97 PA1783 [P] COG2223 Nitrate/nitrite transporter Pae Pseudomonas a... 328 2e-89 BS_nasA [P] COG2223 Nitrate/nitrite transporter Bsu Bacillus sub... 264 3e-70 SA2176 [P] COG2223 Nitrate/nitrite transporter Sau Staphylococcu... 168 3e-41 BS_narK [P] COG2223 Nitrate/nitrite transporter Bsu Bacillus sub... 166 8e-41 >CC0614 Non COG Length = 473 Score = 353 bits (906), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 215/412 (52%), Positives = 267/412 (64%), Gaps = 17/412 (4%) Query: 18 EFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLAGALL 77 EF KAGH PTL AAFLYFD+SFM WV LGPL V IA+D NL +K +VA+PVLAGA+L Sbjct: 72 EFLKAGHAPTLFAAFLYFDLSFMVWVILGPLGVAIAKDFNLDPAQKGLMVAVPVLAGAVL 131 Query: 78 RVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASFAVAL 137 R+ G+L D IG K+TGII QL+V+A W GL + I GL LGV GASFAVAL Sbjct: 132 RLVNGVLVDRIGPKKTGIIGQLIVLAGLVTAWAVGLHNYQQILALGLVLGVAGASFAVAL 191 Query: 138 PQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMVLAYY 197 P ASRWYP ++QG+ +GIAGAGN G L L AP++A+ +GWQ V G IP+ Y Sbjct: 192 PLASRWYPQEHQGLALGIAGAGNSGTALAALFAPALAKTFGWQNVIGLAAIPLAFAFVAY 251 Query: 198 AFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQYHVS 257 AKDAP + L Y +LK D+ W M Y +TFGGFVGLA++L +YF +Y + Sbjct: 252 LVLAKDAPEQPAPKKLAEYLDVLKVPDAWWLMLLYGVTFGGFVGLASSLTMYFNSEYGLD 311 Query: 258 GVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGPTAPT 317 V AG A V GS RPVGG +ADR GG+R+L +++ V+ A L +A G P+ Sbjct: 312 PVIAGFFTAACVFAGSFIRPVGGALADRYGGVRTLGVVY-VLAAIGLTVA----GFQLPS 366 Query: 318 PAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGCAGGIG 377 AG AL +VLLF++ LALG GNGAVFQL P RFR+EIG+MTGL+G GGIG Sbjct: 367 -AGLAL---------AVLLFAM--LALGAGNGAVFQLAPQRFRNEIGVMTGLIGMTGGIG 414 Query: 378 GFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWGAVSG 429 GF+LA TLG AK MTG + +GF+ F LA+ I L +K RWR W + G Sbjct: 415 GFYLASTLGLAKKMTGSYQSGFIGFALLAVFALIALFSLKARWRAVWPTLHG 466 >PA1783 [P] COG2223 Nitrate/nitrite transporter Pae Pseudomonas aeruginosa Length = 403 Score = 328 bits (840), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 215/417 (51%), Positives = 264/417 (63%), Gaps = 19/417 (4%) Query: 18 EFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLAGALL 77 F KAGH PTL AAFLYFD+SFM W LGPL V IA D+ L+ ++ +VA P+LAGA+L Sbjct: 4 SFWKAGHAPTLFAAFLYFDLSFMVWYVLGPLGVQIAGDLQLSTQQRAMMVATPILAGAVL 63 Query: 78 RVPLGILADIIGAKRTGIIAQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASFAVAL 137 R LG+LAD K GI+AQ++VIAA AA W G+ S L GL LG GASFAVAL Sbjct: 64 RFLLGLLADRWSPKSAGILAQVIVIAALAAAWLLGVNSYAQALLLGLFLGFAGASFAVAL 123 Query: 138 PQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMVLAYY 197 P AS+WYPPQ+QG MGIAGAGN G VL L AP +A +GW VFG L+P+++ L + Sbjct: 124 PLASQWYPPQHQGKAMGIAGAGNSGTVLAALFAPGLAAAFGWNNVFGFALLPLVLTLLVF 183 Query: 198 AFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTVQYHVS 257 A A++AP ++ Y L D DS WFMFFY +TFGGF+GLA+ LP YF QY ++ Sbjct: 184 AMVARNAPQRPAPKAMGDYLKALGDRDSWWFMFFYSVTFGGFLGLASTLPGYFHDQYGLN 243 Query: 258 GVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGPTAPT 317 V AG A V GS RP+GG +ADRIGGIRSL +++ +AA + A P+A Sbjct: 244 PVTAGYYTAACVFAGSLMRPLGGALADRIGGIRSLLMVY--TLAAICIAAVGFHLPSAMA 301 Query: 318 PAGWALIELPRIAWISVLLFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGCAGGIG 377 G LF + +L+LG GNGAVFQL+P RF EIG+MTGL+G AGGIG Sbjct: 302 ALG---------------LFVVAMLSLGAGNGAVFQLVPQRFHKEIGVMTGLIGMAGGIG 346 Query: 378 GFFLAQTLGFAKGMTGGFGAGFLFFGGLALLGFIGLAMVKVRWRTTWG--AVSGARV 432 GF L LG K TG + G F L +L + GL VK RWRTTWG AV+ ARV Sbjct: 347 GFCLTAGLGAIKQSTGDYQLGLWLFASLGVLAWFGLHGVKRRWRTTWGSAAVTAARV 403 >BS_nasA [P] COG2223 Nitrate/nitrite transporter Bsu Bacillus subtilis Length = 421 Score = 264 bits (675), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 143/299 (47%), Positives = 194/299 (64%), Gaps = 3/299 (1%) Query: 14 MKLAEFKKAGHWPTLLAAFLYFDISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLA 73 MKL+E K +GH TLL +FLYFD+SFM WV LG L VYI++D L+ EK +VA+P+L+ Sbjct: 1 MKLSELKTSGHPLTLLCSFLYFDVSFMIWVMLGALGVYISQDFGLSPFEKGLVVAVPILS 60 Query: 74 GALLRVPLGILADIIGAKRTGIIAQLVVIAATAAVW-HFGLTSKLAIELFGLALGVGGAS 132 G++ R+ LGIL D IG K+T +I LV + +W FG S + G+ LGV GAS Sbjct: 61 GSVFRIILGILTDRIGPKKTAVIGMLVTM--IPLLWGTFGGRSLTELYAIGILLGVAGAS 118 Query: 133 FAVALPQASRWYPPQYQGVVMGIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLM 192 FAVALP ASRWYPP QG+ MGIAGAGN G + TL P +AE +GW V G LIP+L+ Sbjct: 119 FAVALPMASRWYPPHLQGLAMGIAGAGNSGTLFATLFGPRLAEQFGWHIVMGIALIPLLI 178 Query: 193 VLAYYAFAAKDAPGERKKVSLQAYGALLKDSDSRWFMFFYFITFGGFVGLANALPLYFTV 252 V + AKD+P + L++Y + ++ +F Y +TFGGFVGL++ L ++F Sbjct: 179 VFILFVSMAKDSPAQPSPQPLKSYLHVFGQKETWFFCLLYSVTFGGFVGLSSFLSIFFVD 238 Query: 253 QYHVSGVAAGMLVALIVAFGSGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPE 311 QY +S + AG V L VA GS FRPVGG+I+DR+GG + LS+LF +V ++ +P Sbjct: 239 QYQLSKIHAGDFVTLCVAAGSFFRPVGGLISDRVGGTKVLSVLFVIVALCMAGVSSLPS 297 >SA2176 [P] COG2223 Nitrate/nitrite transporter Sau Staphylococcus aureus N315 Length = 389 Score = 168 bits (425), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 117/393 (29%), Positives = 197/393 (50%), Gaps = 25/393 (6%) Query: 37 ISFMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLAGALLRVPLGILADIIGAKRTGII 96 + FMAW + PL+ +I +D+N+ + ++AIPV+ G++LRVP G L +I+GAK Sbjct: 19 VGFMAWSIIAPLMPFIKQDVNVTEGQISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFT 78 Query: 97 AQLVVIAATAAVWHFGLTSKLAIELFGLALGVGGASFAVALPQASRWYPPQYQGVVMGIA 156 + +V++ + L G LGVGGA F+V + +++P + G+ GI Sbjct: 79 SFIVLLFPIFFLSQAQTPGMLMAS--GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIY 136 Query: 157 GAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMVLAYYAFAAKDAPGERKKVSLQAY 216 G GN+G + + LAP IA GWQ LI ++ + A F D + KV L A Sbjct: 137 GMGNIGTAVSSFLAPPIAGIIGWQTTVRSYLI-IIALFALIMFIFGDTQERKIKVPLMAQ 195 Query: 217 GALLKDSDSRWFM-FFYFITFGGFVGLANALPLYFTVQYHVSGVAAGMLVALIVAFGSGF 275 L + +++ ++YFITFG FV LP Y + + V AG+ + +A + Sbjct: 196 MKTLSKNYKLYYLSYWYFITFGAFVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFL 255 Query: 276 RPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGPTAPTPAGWALIELPRIAWISVL 335 RP+GG++ D+ ++ L I F +++ +++ IA +V Sbjct: 256 RPIGGILGDKFNAVKVLMIDFVIMIIGAVILGISDH-----------------IALFTVG 298 Query: 336 LFSIGVLALGMGNGAVFQLIPLRFRHEIGLMTGLVGCAGGIGGFFLAQTLGFAKGMTGGF 395 +I + A G+GNG +F+L+P F +E G G+V GG+GGFF + + +TG Sbjct: 299 CLTISICA-GIGNGLIFKLVPSYFSNEAGSANGIVSMMGGLGGFFPPLVITYVANLTGSS 357 Query: 396 GAGFLFFGGLALLGFIGLAMVKVRWRTTWGAVS 428 F+F LA+ G I L ++ ++ +G++ Sbjct: 358 HLAFIF---LAVFGCIALFTMRHLYQKEYGSLK 387 >BS_narK [P] COG2223 Nitrate/nitrite transporter Bsu Bacillus subtilis Length = 395 Score = 166 bits (421), Expect = 8e-41, Method: Compositional matrix adjust. Identities = 119/383 (31%), Positives = 192/383 (50%), Gaps = 40/383 (10%) Query: 39 FMAWVALGPLIVYIARDMNLAVNEKFTLVAIPVLAGALLRVPLGILADIIGAKRTGIIAQ 98 FM WV + LI I D++L+ E + AIPV+ G+LLR+PLG L + GA+ +++ Sbjct: 20 FMVWVLISSLISQITLDIHLSKGEISLVTAIPVILGSLLRIPLGYLTNRFGARLMFMVSF 79 Query: 99 LVVIAATAAVWHFGLTSKLAIELF-----GLALGVGGASFAVALPQASRWYPPQYQGVVM 153 ++++ + +A LF G LG+GGA F++ + ++YP + GVV Sbjct: 80 ILLLFPVFWI-------SIADSLFDLIAGGFFLGIGGAVFSIGVTSLPKYYPKEKHGVVN 132 Query: 154 GIAGAGNMGVVLDTLLAPSIAEHWGWQAVFGCLLIPMLMVLAYYAFAAKDAPGERKKVSL 213 GI GAGN+G + T AP IA+ GW++ LI +L V A D ++ KVS+ Sbjct: 133 GIYGAGNIGTAVTTFAAPVIAQAVGWKSTVQMYLI-LLAVFALLHVLFGDRHEKKVKVSV 191 Query: 214 QAYGALLKDSDSRWFM-FFYFITFGGFVGLANALPLYFTVQYHVSGVAAGMLVALIVAFG 272 + + + WF+ FYFITFG FV LP + + ++ AG+ A +A Sbjct: 192 KTQIKAVYRNHVLWFLSLFYFITFGAFVAFTIYLPNFLVEHFGLNPADAGLRTAGFIAVS 251 Query: 273 SGFRPVGGMIADRIGGIRSLSILFGVVVAAYLVIAFMPEGPTAPTPAGWALIELPRIAWI 332 + RP GG +AD++ +R L +F + + ++++F P Sbjct: 252 TLLRPAGGFLADKMSPLRILMFVFTGLTLSGIILSFSP---------------------- 289 Query: 333 SVLLFSIGVLAL----GMGNGAVFQLIPLRFRHEIGLMTGLVGCAGGIGGFFLAQTLGFA 388 ++ L++ G L + G+GNG VF+L+P F + G+ G+V GG+GGFF L Sbjct: 290 TIGLYTFGSLTVAVCSGIGNGTVFKLVPFYFSKQAGIANGIVSAMGGLGGFFPPLILASV 349 Query: 389 KGMTGGFGAGFLFFGGLALLGFI 411 TG + GF+ +AL F+ Sbjct: 350 FQATGQYAIGFMALSEVALASFV 372