BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0228.0009.p (208 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll7642 [E] COG1280 Putative threonine efflux protein Mlo Mesorh... 221 8e-58 RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralsto... 144 1e-34 BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillu... 136 3e-32 DR1999 [E] COG1280 Putative threonine efflux protein Dra Deinoco... 135 7e-32 MA1855 [E] COG1280 Putative threonine efflux protein Mac Methano... 133 3e-31 >mll7642 [E] COG1280 Putative threonine efflux protein Mlo Mesorhizobium loti Length = 208 Score = 221 bits (563), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 110/208 (52%), Positives = 149/208 (71%) Query: 1 MIDITTLLTYAAVVLGLFLIPGPAVLLVLARSVTGGARVGVATGFGIALGDLVHTAMATF 60 MID++T + Y AVVLG IPGPA LL +AR+ + G +VG+ATG GIA GD+ HT MA Sbjct: 1 MIDLSTSIAYTAVVLGFVFIPGPATLLTIARATSSGTKVGIATGAGIAAGDIFHTVMAIL 60 Query: 61 GLSAVLMTSALAFSVVKYAGVIYLIVLGVRALLEKNGDLNLPAAQPVDPRRAFRQAIWAE 120 G+SA++ TSA FS+VKY G YL+ LG+RA++EK A + +AFRQA+ E Sbjct: 61 GISAIIATSATLFSIVKYIGAAYLVYLGIRAIIEKTPADPSAGALAISAGKAFRQAVLTE 120 Query: 121 MLNPKTALFFLAFLPQFVHPGHGSAVAQFATLGLIFVVMSAVYTSLLALAAGQISPWIRR 180 +LNPKTALFFLAFLPQFV P +G+ + Q A LG++FVV+ T + A++AG++ ++RR Sbjct: 121 VLNPKTALFFLAFLPQFVRPENGTVMLQLAVLGIVFVVLGLFSTVVFAVSAGRLGSFLRR 180 Query: 181 HRRIGQWQGRVVGTIYIALGVRLAFQER 208 + + +WQG+VVG IY ALGVRLA Q+R Sbjct: 181 NPTVLKWQGKVVGGIYCALGVRLALQQR 208 >RSc0073 [E] COG1280 Putative threonine efflux protein Rso Ralstonia solanacearum Length = 215 Score = 144 bits (363), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 89/212 (41%), Positives = 116/212 (54%), Gaps = 5/212 (2%) Query: 2 IDITTLLTYAAVVLGLFLIPGPAVLLVLARSVTGGARVGVATGFGIALGDLVHTAMATFG 61 + I L + VL L + PGP ++ RSV+ G G+ + G++ G VH FG Sbjct: 4 LGIINLPVFMLAVLLLNVTPGPDTAYIVGRSVSQGRAAGLMSSLGVSAGCCVHVLAVAFG 63 Query: 62 LSAVLMTSALAFSVVKYAGVIYLIVLGVRALLEKNGDLNLPAAQPV----DPRRA-FRQA 116 L+A+L SA+AF+V+K G YLI LG R LL G + P AQ V P RA F Q Sbjct: 64 LTALLAASAVAFTVIKAIGAAYLIYLGGRMLLAPAGSDDAPVAQAVATARRPLRALFMQG 123 Query: 117 IWAEMLNPKTALFFLAFLPQFVHPGHGSAVAQFATLGLIFVVMSAVYTSLLALAAGQISP 176 +LNPK LFFL+F PQFV PG A F LG +F+VMS + SL+A A ++ Sbjct: 124 FLTNVLNPKVVLFFLSFFPQFVDPGASHKAAAFLVLGAVFIVMSTAWNSLVAWVAASVTR 183 Query: 177 WIRRHRRIGQWQGRVVGTIYIALGVRLAFQER 208 + I +W RVVGT +I LG RLAF R Sbjct: 184 RVAGKPGIKRWLDRVVGTAFIGLGARLAFTTR 215 >BH0429 [E] COG1280 Putative threonine efflux protein Bha Bacillus halodurans Length = 207 Score = 136 bits (343), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 77/207 (37%), Positives = 124/207 (59%) Query: 2 IDITTLLTYAAVVLGLFLIPGPAVLLVLARSVTGGARVGVATGFGIALGDLVHTAMATFG 61 +D+T+LL++ V + L L+PGP +L VLA+S++ + G+ T G+ G LVH A G Sbjct: 1 MDLTSLLSFLGVAVLLTLMPGPDILFVLAQSMSQNRQAGIVTALGLCTGLLVHITAAVVG 60 Query: 62 LSAVLMTSALAFSVVKYAGVIYLIVLGVRALLEKNGDLNLPAAQPVDPRRAFRQAIWAEM 121 +SA++ SALAF+VVKYAG YL+ L +A EK L++ + +++ I + Sbjct: 61 ISALIYQSALAFTVVKYAGAAYLLYLAWKAFQEKGEGLSIDKQTTLAYGALYKKGIIMNV 120 Query: 122 LNPKTALFFLAFLPQFVHPGHGSAVAQFATLGLIFVVMSAVYTSLLALAAGQISPWIRRH 181 LNPK +LFFLA LPQFV+ G GSA Q LG++F++ + + SL++ A ++ + R Sbjct: 121 LNPKVSLFFLALLPQFVNSGAGSAPWQMLLLGVVFLIQAFIIFSLVSWFAEKVGQLLMRS 180 Query: 182 RRIGQWQGRVVGTIYIALGVRLAFQER 208 I R+ G + +G+++AF + Sbjct: 181 SFIKNQMHRIKGGLLALIGLQVAFSKN 207 >DR1999 [E] COG1280 Putative threonine efflux protein Dra Deinococcus radiodurans Length = 241 Score = 135 bits (340), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 87/212 (41%), Positives = 117/212 (55%), Gaps = 5/212 (2%) Query: 1 MIDITTLLTYAAVVLGLFLIPGPAVLLVLARSVTGGARVGVATGFGIALGDLVHTAMATF 60 MID + + L L+PGP ++ +LARS+ GG G+ + G G +VH + Sbjct: 30 MIDPHQYGAFLVAAVVLALLPGPGLMYILARSLGGGRWAGIQSALGTGAGGMVHVLASAV 89 Query: 61 GLSAVLMTSALAFSVVKYAGVIYLIVLGVRALLEKNG-----DLNLPAAQPVDPRRAFRQ 115 GLSA++M S+LAFSVVKYAG YLI LG+R LL K + +L AA P R F Q Sbjct: 90 GLSALIMASSLAFSVVKYAGAAYLIYLGLRVLLSKEALSSKEEASLAAAAPERQSRLFTQ 149 Query: 116 AIWAEMLNPKTALFFLAFLPQFVHPGHGSAVAQFATLGLIFVVMSAVYTSLLALAAGQIS 175 E+LNPKTALFFLA +PQFV P G QF LG VV++ + L+A AG + Sbjct: 150 GAMTELLNPKTALFFLAVIPQFVKPATGHVFGQFLLLGTTSVVVNTLNAMLVATLAGFLG 209 Query: 176 PWIRRHRRIGQWQGRVVGTIYIALGVRLAFQE 207 ++ + R + Q G IALG +A + Sbjct: 210 ARLQGNPRFQRGQKVASGGAMIALGTYVAVER 241 >MA1855 [E] COG1280 Putative threonine efflux protein Mac Methanosarcina acetivorans str.C2A Length = 211 Score = 133 bits (334), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 82/211 (38%), Positives = 123/211 (58%), Gaps = 6/211 (2%) Query: 1 MIDITTLLTYAAVVLGLFLIPGPAVLLVLARSVTGGARVGVATGFGIALGDLVHTAMATF 60 +I+ + L+ + A L +PGP +L VL +S++ G G+AT G+ G LVHT+ A Sbjct: 4 LIEQSQLIYFIAASAALTFLPGPDILFVLTQSISQGKMAGIATATGLCTGILVHTSAAAL 63 Query: 61 GLSAVLMTSALAFSVVKYAGVIYLIVLGVRALLEKNGDLNLPAAQPVDPRRA---FRQAI 117 G+SA+L SALAF +VKYAG YL+ L +AL E L ++ PV A +R+ I Sbjct: 64 GISALLYKSALAFEIVKYAGAAYLLYLAWQALKESG---ELVSSAPVRETNALALYRRGI 120 Query: 118 WAEMLNPKTALFFLAFLPQFVHPGHGSAVAQFATLGLIFVVMSAVYTSLLALAAGQISPW 177 + +LNPK ALFFLAFLPQFV+ GS Q LG++F++ + + S +++ AG I Sbjct: 121 FMNVLNPKVALFFLAFLPQFVNLESGSVPIQMIFLGIVFLIQAWLIFSSISIFAGIIGDK 180 Query: 178 IRRHRRIGQWQGRVVGTIYIALGVRLAFQER 208 I + IG++ I+ +GV+LA + Sbjct: 181 IVQRPGIGKYINWGKAGIFTVIGVKLALSHK 211