BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0224.0020.p (381 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC1311 Non COG 268 1e-71 CC1807 Non COG 253 5e-67 RSc2044 [I] COG1960 Acyl-CoA dehydrogenases Rso Ralstonia solana... 233 5e-61 CC2050 Non COG 184 4e-46 BS_yngJ [I] COG1960 Acyl-CoA dehydrogenases Bsu Bacillus subtilis 173 6e-43 >CC1311 Non COG Length = 401 Score = 268 bits (685), Expect = 1e-71, Method: Compositional matrix adjust. Identities = 150/377 (39%), Positives = 227/377 (60%), Gaps = 4/377 (1%) Query: 1 MDLTLSDEQRLLRESADRFVAETFDPEHRKKAANDPLGYSPAIWKQFAE-LGWLALPISE 59 MD ++EQ ++R+S F+ + +D + R+K + G+ WK FAE LG L SE Sbjct: 23 MDFNFTEEQSMVRDSVASFLQDKYDFDTRRKIVSSDAGWRADYWKAFAEELGILGASFSE 82 Query: 60 AHGGLGGGAIEIGLLMEAFGRGLVSEPFLSTVVIGAALVAECGTEAQKQATLPKIAEGAL 119 GGLGGGAI+ ++ME FG+ LV EP+L TVVIG + G A + + I G+ Sbjct: 83 ELGGLGGGAIDNMIIMEEFGKALVIEPYLGTVVIGGGFMKHSGY-AGAASVIEGIVGGST 141 Query: 120 TLAFAHSERAARFDLGYVDTTAGKTADGWRLNGSKIAVLDGAAADQIIVSARIANANGAS 179 T+AFA++E AR+ + TTA K GW LNG K V+ A +IV+AR A + Sbjct: 142 TIAFAYAEPQARYTWQDLKTTAKKDGSGWVLNGHKAVVVGAPFATHLIVTARTGGAQRDA 201 Query: 180 GKQGLFLVPAGTPGLTLRDYARLGGGRGCNLDLKDVQLPADALLGDGTDALPAIETVVDR 239 G G+F++ PG+ RDY + G R + ++V +PA+AL+ + AL +E V+D Sbjct: 202 GGVGVFIIDKSLPGVVTRDYPTVDGNRASEVYFENVSIPAEALISESGLAL--VEQVMDE 259 Query: 240 AMAALGAEAVGIMQTLLDTTLEYTKIRKQFGRPLSANQVIRHRLADVAMQVDEARSMALR 299 A+AA+GAEAVG+++ L + TL+Y K RKQFG ++ QV++HR+ D+ ++V+++ SM Sbjct: 260 AIAAVGAEAVGVLRKLHEGTLDYAKQRKQFGTAIANFQVLQHRMVDMFIEVEQSVSMTYM 319 Query: 300 AALMANAEPGARGRAASGAKAKIGKCARFVAEQAVQLHGAMGVTEELDIGAYFKRLLAFD 359 A + R +A S K +IG+ +FV + A+Q+HG MG+T+EL IG YFKR + Sbjct: 320 ATIKVGESAAERAKAVSAMKVRIGRACKFVGQSAIQIHGGMGMTDELAIGHYFKRATMIE 379 Query: 360 TLFGNSAHHYRRHAALS 376 LFG+ HH +R+ +LS Sbjct: 380 GLFGSVDHHLKRYESLS 396 >CC1807 Non COG Length = 382 Score = 253 bits (646), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 150/368 (40%), Positives = 207/368 (56%), Gaps = 1/368 (0%) Query: 1 MDLTLSDEQRLLRESADRFVAETFDPEHRKKAANDPLGYSPAIWKQFAELGWLALPISEA 60 M+ LS+EQ+LL++SA R+ A+ D + Y A W++ A+LGWL L + E Sbjct: 1 MNFDLSEEQQLLKDSARRYGADHGDFTAWRARVERGEAYDAASWRRMADLGWLMLNVPED 60 Query: 61 HGGLGGGAIEIGLLMEAFGRGLVSEPFLSTVVIGAALVAECGTEAQKQATLPKIAEGALT 120 GGLG G E ++ EA GR L+ PF+ST VI L+ TEA + + +AEG L Sbjct: 61 DGGLGAGPTETMVVAEAVGRYLMLVPFVSTGVIAPTLLT-AATEALRAHLMAGLAEGKLV 119 Query: 121 LAFAHSERAARFDLGYVDTTAGKTADGWRLNGSKIAVLDGAAADQIIVSARIANANGASG 180 ++ A +E RFDL + T A G+RL G+K LDG AAD IV AR A+ + Sbjct: 120 ISLADAEPNGRFDLNRIATRAEAVEGGFRLTGAKSHALDGGAADWFIVPARTTAADDSQD 179 Query: 181 KQGLFLVPAGTPGLTLRDYARLGGGRGCNLDLKDVQLPADALLGDGTDALPAIETVVDRA 240 LFLVPA GLT+ + +L L V +PA L+G + P + VDR Sbjct: 180 GVSLFLVPAEIEGLTVMRSRAMDNRHNASLKLDGVVVPAANLIGGLGEGFPLLRDAVDRG 239 Query: 241 MAALGAEAVGIMQTLLDTTLEYTKIRKQFGRPLSANQVIRHRLADVAMQVDEARSMALRA 300 + A AEAVG M + + T+EY K RKQFG+ L A Q ++HR D+A+ +EARSM A Sbjct: 240 VVARLAEAVGAMDAVREMTMEYLKTRKQFGQTLGAFQALQHRAVDMAIACEEARSMMYLA 299 Query: 301 ALMANAEPGARGRAASGAKAKIGKCARFVAEQAVQLHGAMGVTEELDIGAYFKRLLAFDT 360 L + P R + + AKA++G+ + +V QAVQLHG +G TEEL + Y KRL+ D Sbjct: 300 TLALSGAPVERRKVIAAAKARVGQTSLYVGRQAVQLHGGVGFTEELAVAHYLKRLIMIDM 359 Query: 361 LFGNSAHH 368 FGN+ HH Sbjct: 360 AFGNADHH 367 >RSc2044 [I] COG1960 Acyl-CoA dehydrogenases Rso Ralstonia solanacearum Length = 376 Score = 233 bits (594), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 149/378 (39%), Positives = 213/378 (56%), Gaps = 8/378 (2%) Query: 1 MDLTLSDEQRLLRESADRFVAETFDPEHRKKAANDPLGYSPAIWKQFAELGWLALPISEA 60 MD + +DEQ+ L ++ RF+ + + E R K P G S A W AELG ALP+ +A Sbjct: 1 MDFSFTDEQKQLADAVRRFIDKDYGFEARNKVVYSPAGVSQAHWDALAELGLTALPVPQA 60 Query: 61 HGGLGGGAIEIGLLMEAFGRGLVSEPFLSTVVIGAALVAECGTEAQKQATLPKIAEGALT 120 GG G A+++ ++M+ GRGLV EP+ +TV+ AL G E A L +A GAL Sbjct: 61 QGGFDGSAMDLLVVMQELGRGLVVEPYAATVLGMQALKLAGGQE----ALLEPVAGGALK 116 Query: 121 LAFAHSERAARFDLGYVDTTAGKTADGWRLNGSKIAVLDGAAADQIIVSARIANANGASG 180 LA A E +R++L V T A + GW L+G+K V+ G AD+++VSAR + Sbjct: 117 LAAAFGEPQSRYELFNVTTRAARQGGGWTLSGAKAVVVHGGQADKLVVSARTGGEARDTS 176 Query: 181 KQGLFLVPAGTPGLTLRDYARLGGGRGCNLDLKDVQLPADALLGDGTDALPAIETVVDRA 240 LFLV G+T++DY + R ++ + PA LLG +A I+ V D Sbjct: 177 GLSLFLVDREAAGVTVKDYRTIDNLRAADVRFDNA--PA-TLLGQAGEAWEIIDAVADLG 233 Query: 241 MAALGAEAVGIMQTLLDTTLEYTKIRKQFGRPLSANQVIRHRLADVAMQVDEARSMA-LR 299 L AEA+G++ L TLEYTK R+QFG P++ Q ++HR+ D+ + ++ARS+ L Sbjct: 234 CVLLCAEAIGLIDALNAATLEYTKTRQQFGVPIARFQALQHRMVDMFIHAEQARSITYLA 293 Query: 300 AALMANAEPGARGRAASGAKAKIGKCARFVAEQAVQLHGAMGVTEELDIGAYFKRLLAFD 359 AA + + AR R S AKA++G+ AR V ++AVQLHG MGVT EL FKRL + Sbjct: 294 AAHFEDGDAEARRRYVSAAKARVGQAAREVGQEAVQLHGGMGVTNELPAAHMFKRLTMIN 353 Query: 360 TLFGNSAHHYRRHAALSG 377 T G+ HH R A+ G Sbjct: 354 TTLGDVDHHLARFASRPG 371 >CC2050 Non COG Length = 377 Score = 184 bits (466), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 136/381 (35%), Positives = 203/381 (53%), Gaps = 16/381 (4%) Query: 5 LSDEQRLLRESADRFVAET--FDPEHRKKAANDPLGYSPAIWKQFAELGWLALPISEAHG 62 L++EQ +LR++A + +E+ + + + + PA W + ++GW + + EA+ Sbjct: 4 LTEEQTMLRDAAKGWASESAPVGALRKLRDSKSKQTFDPAAWAEMGQMGWAGVIVPEAYD 63 Query: 63 GLGGGAIEIGLLMEAFGRGLVSEPFLSTVVIGAALVAECGTEAQKQATLPKIAEGALTLA 122 G G + +GL++E GR L + P LST +I A+ + G++AQKQA LPKIA G Sbjct: 64 GSAFGYLGLGLILEETGRTLAASPLLSTAMIAASALQLGGSDAQKQAWLPKIATGEAVAT 123 Query: 123 FAHSERA----ARFDLGYVDTTAGKTADGWRLNGSKIAVLDGAAADQIIVSARIANANGA 178 A E A AR L +A ++ G+ LNG+K VLDG AAD +IV+AR + A G Sbjct: 124 LAIDEGAHHAPARTAL-----SATQSGAGYVLNGTKTLVLDGEAADLLIVAARTSGAPGD 178 Query: 179 SGKQGLFLVPAGTPGLTLRDYARLGGGRG-CNLDLKDVQLPADALLGDGTDALPAIETVV 237 + LFLV G+T R + L RG + V++ ADA+LG+ +E + Sbjct: 179 TNGITLFLVAGDAAGVT-RSHLSLIDSRGAAKIAFDGVEVGADAVLGEVDKGWAVLEPTL 237 Query: 238 DRAMAALGAEAVGIMQTLLDTTLEYTKIRKQFGRPLSANQVIRHRLADVAMQVDEARSMA 297 DRA A L AE +G D TL+Y K R QFG+ + Q ++HR A ++ RS Sbjct: 238 DRAYAGLAAEMLGSASAAFDITLDYLKTRTQFGQVIGTFQALQHRAAKWFTDLETTRS-C 296 Query: 298 LRAALMANAEPGARGR-AASGAKAKIGKCARFVAEQAVQLHGAMGVTEELDIGAYFKRLL 356 + AAL A + GA R A AKAK + + + VQ+HG +G+T+ D G Y KR Sbjct: 297 VEAALEA-LDAGADSRALAFLAKAKASELVHLASNEMVQMHGGIGMTDAHDAGLYMKRAR 355 Query: 357 AFDTLFGNSAHHYRRHAALSG 377 + LFG ++ H R+A L G Sbjct: 356 VTEALFGGASFHRDRYARLMG 376 >BS_yngJ [I] COG1960 Acyl-CoA dehydrogenases Bsu Bacillus subtilis Length = 380 Score = 173 bits (439), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 119/355 (33%), Positives = 175/355 (49%), Gaps = 12/355 (3%) Query: 1 MDLTLSDEQRLLRESADRFVAETFDP--EHRKKAANDPLGYSPAIWKQFAELGWLALPIS 58 M+ L+ EQ+++RE F + P EH + P+ +K+ ELG + +P Sbjct: 1 MNFELTKEQQMVREMVKDFAKKEIAPHAEHVDQTGEFPM----QTFKKMGELGLMGIPFP 56 Query: 59 EAHGGLGGGAIEIGLLMEAFGR--GLVSEPFLSTVVIGAALVAECGTEAQKQATLPKIAE 116 E +GG GG I L +E G+ G + + V +GA+ + GTE QKQ L +A Sbjct: 57 EEYGGSGGDTISYALAVEEVGKACGSTGLSYAAAVSLGASPLYYFGTEEQKQTHLTPLAS 116 Query: 117 GALTLAFAHSERAARFDLGYVDTTAGKTADGWRLNGSKIAVLDGAAADQIIVSARIANAN 176 G +F +E A D G T A D + +NG K + + + A +IV+A Sbjct: 117 GTALGSFGLTEPNAGSDAGGTQTKAISNGDEYVINGEKCWITNASYARTVIVTAVTGKNK 176 Query: 177 GASGKQGLFLVPAGTPGLTLRDYARLGGGRGCN---LDLKDVQLPADALLGDGTDALPAI 233 +VP TPGLT+ G RG N + L+DV++PA LLGD T Sbjct: 177 DGKNIISALIVPTDTPGLTITSPYDKMGVRGSNTAEILLEDVRVPAANLLGDPTKGFKQF 236 Query: 234 ETVVDRAMAALGAEAVGIMQTLLDTTLEYTKIRKQFGRPLSANQVIRHRLADVAMQVDEA 293 +D ++ A AVGI Q LD +L Y K RKQFG+P+S+ Q I+ +LAD+AM++D A Sbjct: 237 LYTLDGGRISIAALAVGIAQAALDASLAYAKERKQFGQPISSFQAIQFKLADMAMEIDLA 296 Query: 294 RSMALRAALMANAEPGARGRAASGAKAKIGKCARFVAEQAVQLHGAMGVTEELDI 348 R M L+AA + + AA AK + A QA+Q+HG G +E + Sbjct: 297 RQMVLKAAWLKDHNRPFTKEAAY-AKLFASEMATRACNQAIQIHGGSGYMKEYGV 350