BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0223.0007.p (427 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value hipA [R] COG3550 Uncharacterized protein related to capsule bios... 215 1e-55 RSc1446 [R] COG3550 Uncharacterized protein related to capsule b... 188 2e-47 ZhipA [R] COG3550 Uncharacterized protein related to capsule bio... 155 2e-37 CC2735 Non COG 126 9e-29 ECs2115 [R] COG3550 Uncharacterized protein related to capsule b... 86 2e-16 >hipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Eco Escherichia coli K12 Length = 440 Score = 215 bits (548), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 142/424 (33%), Positives = 219/424 (51%), Gaps = 29/424 (6%) Query: 4 ELIALLDGREVGRVHNDARGRLTFVYDSDWRQTRDAYPISLSMPLAAEEHGPAAVQAFLW 63 +L+ ++ + VG + A G TF Y +W +R A P+SLS+PL AV F Sbjct: 3 KLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFD 62 Query: 64 GLLPDNERVLDRWAKKFQVSARNVFALISHVGEDCAGAIQFVTPDRLEKLQSGTDDKVEW 123 LLPD+ V DR K++ +R F L+S +G D GA+ + D ++ T + W Sbjct: 63 NLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVTLIPED-----ETVTHPIMAW 117 Query: 124 --LDEAAVAKRLQALREDHAAWRLPRDTGQF--SLAGAQPKTALLLQRGQWGIPSGRIPT 179 L EA + + L A + D + R+ F S+AGAQ KTALL W IP G PT Sbjct: 118 EKLTEARLEEVLTAYKAD-IPLGMIREENDFRISVAGAQEKTALLRIGNDWCIPKGITPT 176 Query: 180 THILKPPTGY---------FDGHAENEHICLNLARNLGLPVAETKVMRFENEVAIVVERY 230 THI+K P G +NE+ CL LA+ LGL V + ++++ N A+ VER+ Sbjct: 177 THIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERF 236 Query: 231 DRQFSGNHVI--RVHQEDVCQARGIMPTRKYQSEGGPSATDIIELLRTHSTESVDDIDTF 288 DR+++ + R+ QED+CQ G+ + KY+S+GGP I+ L S+E++ D F Sbjct: 237 DRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFL-MGSSEALKDRYDF 295 Query: 289 IDALGFNWLIGGTDAHAKNYSLLLANGPTVRLAPLYDIASILPY---DDVDLQKMKLAMK 345 + F WLIG TD HAKN+S+ + G + RL P YDI S P + + +KLAM Sbjct: 296 MKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMG 355 Query: 346 I----GGEYKLGQIGAREWRKFAREIRFDAEKVIAGLNSLADLLPDSVSGVCATAQAEGL 401 + G + + +I R + A+ +RF ++ L+ A ++P ++ V + + Sbjct: 356 LNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIPAALDNVKTSLPTDFP 415 Query: 402 DNAV 405 +N V Sbjct: 416 ENVV 419 >RSc1446 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Rso Ralstonia solanacearum Length = 445 Score = 188 bits (477), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 140/423 (33%), Positives = 211/423 (49%), Gaps = 43/423 (10%) Query: 4 ELIALLDGREVGRVHNDARGRLTFVYDSDWRQTRDAYPISLSMPLAA--EEHGPAAVQAF 61 EL ++G VG G YD W ++ + P+SLS+P+ A E V + Sbjct: 5 ELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDY 64 Query: 62 LWGLLPDNERVLDRWAKKFQVSARNVFALISHVGEDCAGAIQFVTP-------DRLEKLQ 114 LLP+ + + +R ++F +++ + L++ +G DC GA+Q + P DR++ + Sbjct: 65 FDNLLPEAQPIRERVRRRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDS-E 123 Query: 115 SGTDDKVEWLDEAAVAKRLQALREDHAAWRLPRDTGQFSLAGAQPKTALLLQRGQWGIPS 174 + TD +V+ L A + AL ED D + S+AGAQ KTALL W P Sbjct: 124 ALTDSQVDTLLRNLTA--MPALGEDAGD----PDAFRISIAGAQEKTALLRIGDAWHRPL 177 Query: 175 GRIPTTHILKPPTGYFDG-------HAENEHICLNLARNLGLPVAETKVMRFENEVAIVV 227 G PTTHILK P G +NE +C L GL VA T++ RF + V Sbjct: 178 GATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAV 237 Query: 228 ERYDRQF-SGNHVI-RVHQEDVCQARGIMPTRKYQSEGGPSATDIIELLRTHSTESVDDI 285 ER+DR++ GN I R+ QED CQA G RKY+++GGP ++LL + S ++DD Sbjct: 238 ERFDRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLL-SASEHAIDDK 296 Query: 286 DTFIDALGFNWLIGGTDAHAKNYSLLLANGPTVRLAPLYDIASILPY-----DDVDLQKM 340 F+ A WL+ TD HAKN+SL + G R+ PLYD+ S+ P + + + Sbjct: 297 RRFVLAQLAFWLLAATDGHAKNFSLAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRA 356 Query: 341 KLAMKIGGE---YKLGQIGAREWRKFARE---------IRFDAEKVIAGLNSLADLLPDS 388 +LAM + G+ +L I R W+ A + ++ E+V AGL + +LP Sbjct: 357 RLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVERVPAGLERVEAILPPD 416 Query: 389 VSG 391 SG Sbjct: 417 FSG 419 >ZhipA [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes EcZ Escherichia coli O157:H7 EDL933 Length = 340 Score = 155 bits (392), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 95/273 (34%), Positives = 146/273 (53%), Gaps = 19/273 (6%) Query: 151 QFSLAGAQPKTALLLQRGQWGIPSGRIPTTHILKPPTGY---------FDGHAENEHICL 201 + S+AGAQ KTALL W IP G PTTHI+K P G +NE+ CL Sbjct: 48 RISVAGAQEKTALLRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCL 107 Query: 202 NLARNLGLPVAETKVMRFENEVAIVVERYDRQFSGNHVI--RVHQEDVCQARGIMPTRKY 259 LA+ LGL V + ++++ N A+ VER+DR+++ + R+ QED+CQ G+ + KY Sbjct: 108 LLAKELGLNVPDAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKY 167 Query: 260 QSEGGPSATDIIELLRTHSTESVDDIDTFIDALGFNWLIGGTDAHAKNYSLLLANGPTVR 319 +S+GGP I+ L S E++ D F+ F WLIG TD HAKN+S+ + G + R Sbjct: 168 ESDGGPGIARIMAFLMGFS-EALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYR 226 Query: 320 LAPLYDIASILPY---DDVDLQKMKLAMKI----GGEYKLGQIGAREWRKFAREIRFDAE 372 L P YDI S P + + +KLAM + G + + +I R + A+ +RF Sbjct: 227 LTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEV 286 Query: 373 KVIAGLNSLADLLPDSVSGVCATAQAEGLDNAV 405 ++ L+ A ++P ++ V + + +N V Sbjct: 287 QMHEILSDFARMIPAALDNVKTSLPTDFPENVV 319 >CC2735 Non COG Length = 444 Score = 126 bits (317), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 118/336 (35%), Positives = 163/336 (48%), Gaps = 24/336 (7%) Query: 5 LIALLDGREV--GRVHNDARGRLTFVYDSDWRQTRDAYPISLSMPLAAEEHGPAAVQAFL 62 LI +DG + G + +D ++F YD D P+SLSMPL G +AF Sbjct: 7 LIVRMDGFNLPAGYLASDEARAISFAYD-DRYIAAGGPPLSLSMPLEQVSFGDVTARAFF 65 Query: 63 WGLLPDN---ERVLDRWAKKFQVSARNVFALISHVGEDCAGAIQF--VTPDRLEKLQSGT 117 LLP+N +RV+DR ++ ++ L+SH+G DC+GAI + D + K+ Sbjct: 66 DNLLPENDQMQRVMDREG----LARDDIVGLLSHLGADCSGAISCLPIGADPI-KVPGVL 120 Query: 118 DDKVEWLDEAAVAKRLQALREDHAAWRLPRD-TGQFSLAGAQPKTALLLQRGQWGIP-SG 175 + E L A+A+ ++L E RLP T +AG Q K AL + P G Sbjct: 121 SEDYELLAPGAIAEIARSLAERQ---RLPDTITDPSPVAGVQRKIALTHTPQGFAKPRPG 177 Query: 176 R-IPTTHILKPPTGYFDGHAENEHICLNLARNLGLPVAETKVMRFENEVAIVVERYDRQF 234 R +PTTHILK P A E LA LGL V+ + + + A+++ R+DR Sbjct: 178 RKVPTTHILKVPETRLRRDARLEAAAARLASALGLDVSIPEAIVIDGVDALLITRFDRVV 237 Query: 235 SGNHVIRVHQEDVCQARGIMPTRKYQSEGGPS----ATDIIELLRTHSTESVDDIDTFID 290 V R+HQED QA G+ T KYQ G P A I +L ++ F+ Sbjct: 238 RDGVVYRLHQEDFAQAMGLPATLKYQRNGAPGRQFDAQAIARVLDQTEAPALSRT-AFLS 296 Query: 291 ALGFNWLIGGTDAHAKNYSLLLANGPTVRLAPLYDI 326 A FN LIG TD HAKN+ LL G LAPLYD+ Sbjct: 297 ATIFNLLIGNTDNHAKNHGLLYRQGRAPILAPLYDL 332 >ECs2115 [R] COG3550 Uncharacterized protein related to capsule biosynthesis enzymes Ecs Escherichia coli O157:H7 Length = 186 Score = 85.5 bits (210), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 52/166 (31%), Positives = 85/166 (51%), Gaps = 8/166 (4%) Query: 247 VCQARGIMPTRKYQSEGGPSATDIIELLRTHSTESVDDIDTFIDALGFNWLIGGTDAHAK 306 +CQ G+ + KY+S+GGP I+ L S E++ D F+ F WLIG TD HAK Sbjct: 1 MCQTFGLPSSVKYESDGGPGIARIMAFLMGFS-EALRDRYDFMKFQVFQWLIGATDGHAK 59 Query: 307 NYSLLLANGPTVRLAPLYDIASILPY---DDVDLQKMKLAMKI----GGEYKLGQIGARE 359 N+S+ + G + RL P YDI S P + + +KLAM + G + + +I R Sbjct: 60 NFSVFIQAGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRH 119 Query: 360 WRKFAREIRFDAEKVIAGLNSLADLLPDSVSGVCATAQAEGLDNAV 405 + A+ +RF ++ L+ A ++P ++ V + + +N V Sbjct: 120 FLATAKVLRFPEVQMHEILSDFARMIPAALDNVKTSLPTDFPENVV 165