BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0219.0002.p (304 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1587 [E] COG0685 5,10-methylenetetrahydrofolate reductase Mlo... 377 e-104 BMEI0559 [E] COG0685 5,10-methylenetetrahydrofolate reductase Bm... 363 e-100 CC2140 Non COG 325 6e-89 AGc3850 [E] COG0685 5,10-methylenetetrahydrofolate reductase Atu... 300 3e-81 STM4105 [E] COG0685 5,10-methylenetetrahydrofolate reductase Sty... 300 3e-81 >mll1587 [E] COG0685 5,10-methylenetetrahydrofolate reductase Mlo Mesorhizobium loti Length = 305 Score = 377 bits (967), Expect = e-104, Method: Compositional matrix adjust. Identities = 182/285 (63%), Positives = 212/285 (74%), Gaps = 1/285 (0%) Query: 19 KISFEFFPPKTEEMERNLWETINRLAPLTPNFVSVTYGAGGSTRERTHSTITRILRETDL 78 K+SFEFFPPK +EME LW+T+ RL PL P FVSVTYGAGGSTRERT T+ RIL ET L Sbjct: 17 KVSFEFFPPKNDEMEARLWDTVTRLEPLKPKFVSVTYGAGGSTRERTARTVGRILNETSL 76 Query: 79 LPAAHLTCVSASRGEIDDIVDRYHEVGVRHIVALRGDPPGGIGTPYFTHPDGYQSSAELV 138 PAAH+TCV A+R ++D ++ + +GV+ VALRGDP G+GT Y HPDGY + AELV Sbjct: 77 TPAAHMTCVDAARHQVDAVIQEFAAMGVKRFVALRGDPAAGVGTAYRPHPDGYANGAELV 136 Query: 139 AGIKRRHADIEISVSAYPEKHPEAKDFDADIDTLKAKVDAGATRAITQVFFDNDLYHRYV 198 +K D +ISVSAYPEKHPE+ DF DID LK KVD GATRAITQ FFDNDLY RYV Sbjct: 137 GAMKD-AGDFDISVSAYPEKHPESPDFATDIDMLKRKVDNGATRAITQFFFDNDLYERYV 195 Query: 199 DRVRARGIEIPIVPGIMPMHNFKQARNFVTRAGTSVPDWLAEKFEGLDNDAETRKLVAAT 258 +R R GI IPIVPGI+P+HNF Q NF R G VP WLAE+FEGL ND +T LVA+ Sbjct: 196 ERARRAGIYIPIVPGILPVHNFTQVANFSARCGALVPAWLAERFEGLQNDPQTHALVASA 255 Query: 259 VAAGQVQKLFKHGVDTFHFYTMNRADLVFAISHLLGIRPNGAQKA 303 VAA QV L + GV FHFYTMNRADLVFAI H++GIR + A+ A Sbjct: 256 VAAEQVLDLVERGVGDFHFYTMNRADLVFAICHMIGIRSHEAEVA 300 >BMEI0559 [E] COG0685 5,10-methylenetetrahydrofolate reductase Bme Brucella melitensis Length = 274 Score = 363 bits (933), Expect = e-100, Method: Compositional matrix adjust. Identities = 174/267 (65%), Positives = 205/267 (76%), Gaps = 1/267 (0%) Query: 32 MERNLWETINRLAPLTPNFVSVTYGAGGSTRERTHSTITRILRETDLLPAAHLTCVSASR 91 ME+ LWET+ RLAPL P+FVSVTYGAGGSTRERT T+ RIL+ETD+ PAAHLTCV A+R Sbjct: 1 MEQRLWETVTRLAPLQPDFVSVTYGAGGSTRERTVRTVARILKETDIKPAAHLTCVDATR 60 Query: 92 GEIDDIVDRYHEVGVRHIVALRGDPPGGIGTPYFTHPDGYQSSAELVAGIKRRHADIEIS 151 ++D + + +GV VALRGDP GIG Y P GYQ+ AELV G+ R AD +IS Sbjct: 61 EDVDRVAREFAALGVNRFVALRGDPANGIGEKYVPTPGGYQNGAELVGGL-RNIADFDIS 119 Query: 152 VSAYPEKHPEAKDFDADIDTLKAKVDAGATRAITQVFFDNDLYHRYVDRVRARGIEIPIV 211 VSAYPEKHPE+ DF DID LK KVD GATRAITQ FF+NDLY RYV+RVR GI IPIV Sbjct: 120 VSAYPEKHPESPDFATDIDMLKRKVDNGATRAITQFFFENDLYERYVERVRRAGIYIPIV 179 Query: 212 PGIMPMHNFKQARNFVTRAGTSVPDWLAEKFEGLDNDAETRKLVAATVAAGQVQKLFKHG 271 PG++P+HNFKQ RNF R+ T +P WLAE+F+GLDND +T +LVAA +AA QV L + G Sbjct: 180 PGVLPVHNFKQVRNFCARSATHIPAWLAERFDGLDNDPQTHQLVAAAIAAEQVMDLIERG 239 Query: 272 VDTFHFYTMNRADLVFAISHLLGIRPN 298 V FHFYTMNRADL FAI H++GIRP Sbjct: 240 VQDFHFYTMNRADLPFAICHMIGIRPK 266 >CC2140 Non COG Length = 314 Score = 325 bits (834), Expect = 6e-89, Method: Compositional matrix adjust. Identities = 168/293 (57%), Positives = 206/293 (70%), Gaps = 7/293 (2%) Query: 18 PKISFEFFPPKTEEMERNLWETINRLAPLTPNFVSVTYGAGGSTRERTHSTITRILRETD 77 P++SFEFFPPKT +ME +LW+ I RLAPL P FVSVTYGAGGSTRERTH T+ RIL ET Sbjct: 23 PRVSFEFFPPKTPQMEESLWQAITRLAPLDPAFVSVTYGAGGSTRERTHRTVKRILDETS 82 Query: 78 LLPAAHLTCVSASRGEIDDIVDRYHEVGVRHIVALRGDPPGGIGTP---YFTHPDGYQSS 134 L PAAHLTCV ASR E+D+++ Y E GVRHIV+LRGDPP G G Y DGY ++ Sbjct: 83 LKPAAHLTCVGASREEVDEVIREYWETGVRHIVSLRGDPPPGEGGIGGVYVPRADGYANA 142 Query: 135 AELVAGIKRRHADIEISVSAYPEKHPEAKDFDADIDTLKAKVDAGATRAITQVFFDNDLY 194 EL + R A E+ V YPEKHPE+ + DID LK KVDAGAT I+Q FFD D + Sbjct: 143 TELTKAV-RAIAPFEVLVGVYPEKHPESPSLEHDIDVLKQKVDAGATLGISQFFFDLDAF 201 Query: 195 HRYVDRVRARGIEIPIVPGIMPMHNFKQARNFVTRAGTSVPDWLAEKFEGLDNDAETRKL 254 R+VD+VRA GI IPIVPGIMP+ NF + T++P WL F+GL+NDAETR+L Sbjct: 202 LRFVDKVRAAGITIPIVPGIMPVTNFAGLKKMAAACQTAIPSWLGNLFDGLENDAETRRL 261 Query: 255 VAATVAAGQVQKLFKHGVDTFHFYTMNRADLVFAISHLLGIR---PNGAQKAA 304 +A +VAA KL + G + FHFYT+NRADLV+AI +LG+R P ++ AA Sbjct: 262 IACSVAAEMCAKLQEQGFEDFHFYTLNRADLVYAICRVLGVREISPAASEVAA 314 >AGc3850 [E] COG0685 5,10-methylenetetrahydrofolate reductase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 312 Score = 300 bits (768), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 154/278 (55%), Positives = 193/278 (69%), Gaps = 1/278 (0%) Query: 19 KISFEFFPPKTEEMERNLWETINRLAPLTPNFVSVTYGAGGSTRERTHSTITRILRETDL 78 ++SFEFFPPK EM+ LWET+ L P+FVSVTYGAGGST+ T + R++ ++ L Sbjct: 29 RLSFEFFPPKNAEMDAQLWETVAELKKWDPDFVSVTYGAGGSTKAPTLDAVRRMIGDSHL 88 Query: 79 LPAAHLTCVSASRGEIDDIVDRYHEVGVRHIVALRGDPPGGIGTPYFTHPDGYQSSAELV 138 AAHLTCV ASR ++ +++ + GVRH VALRGDP GIGT Y HP+GY+++A LV Sbjct: 89 PTAAHLTCVDASRDDVHQVIEEFRNAGVRHFVALRGDPSTGIGTAYQPHPEGYENAAALV 148 Query: 139 AGIKRRHADIEISVSAYPEKHPEAKDFDADIDTLKAKVDAGATRAITQVFFDNDLYHRYV 198 G+ R D EISVSAYPEKHPE+ DID LK K D GATRA+TQ FFDND++ RY+ Sbjct: 149 KGL-REIGDFEISVSAYPEKHPESASETVDIDMLKRKADNGATRALTQFFFDNDVFERYM 207 Query: 199 DRVRARGIEIPIVPGIMPMHNFKQARNFVTRAGTSVPDWLAEKFEGLDNDAETRKLVAAT 258 +RV A GI IP+VPGIMP+ N Q + F R GTSVP +L E+FEG D D E R VAA Sbjct: 208 ERVTAAGITIPVVPGIMPIQNLTQLKRFAGRCGTSVPRFLDERFEGYDEDLEGRARVAAE 267 Query: 259 VAAGQVQKLFKHGVDTFHFYTMNRADLVFAISHLLGIR 296 VAA Q+ L + G + FH YTMNRA LV A+ LG++ Sbjct: 268 VAAEQIADLQRRGFNEFHLYTMNRAPLVDAVLENLGLQ 305 >STM4105 [E] COG0685 5,10-methylenetetrahydrofolate reductase Sty Salmonella typhimurium LT2 Length = 296 Score = 300 bits (768), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 147/278 (52%), Positives = 184/278 (66%), Gaps = 8/278 (2%) Query: 20 ISFEFFPPKTEEMERNLWETINRLAPLTPNFVSVTYGAGGSTRERTHSTITRILRETDLL 79 +SFEFFPP+T EME+ LW +I+RL+ L P FVSVTYGA R+RTHS I I T L Sbjct: 25 VSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRTHSVIKGIKERTGLE 84 Query: 80 PAAHLTCVSASRGEIDDIVDRYHEVGVRHIVALRGDPPGGIGTPYFTHPDGYQSSAELVA 139 A HLTC+ A+R E+ I Y G+RHIVALRGD P G G P D + Sbjct: 85 AAPHLTCIDATRDELRTIARDYWNNGIRHIVALRGDLPPGSGKPEMYAAD--------LV 136 Query: 140 GIKRRHADIEISVSAYPEKHPEAKDFDADIDTLKAKVDAGATRAITQVFFDNDLYHRYVD 199 G+ + AD +ISV+AYPE HPEAK AD+ LK KVDAGA RAITQ FFD + Y R+ D Sbjct: 137 GLLKEVADFDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRD 196 Query: 200 RVRARGIEIPIVPGIMPMHNFKQARNFVTRAGTSVPDWLAEKFEGLDNDAETRKLVAATV 259 R + GI++ I+PGI+P+ NFKQA+ F +P W++ FEGLDNDAETRKLV A + Sbjct: 197 RCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPSWMSLMFEGLDNDAETRKLVGANI 256 Query: 260 AAGQVQKLFKHGVDTFHFYTMNRADLVFAISHLLGIRP 297 A V+ L + GV FHFYT+NRA++ +AI H LG+RP Sbjct: 257 AMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRP 294