BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0215.0008.p (350 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll3840 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 344 2e-94 CC3383 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 278 2e-74 RSc2551 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 237 4e-62 slr0060 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 89 2e-17 mlr2398 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 85 2e-16 >mll3840 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Mlo Mesorhizobium loti Length = 376 Score = 344 bits (882), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/337 (50%), Positives = 230/337 (68%), Gaps = 2/337 (0%) Query: 9 RAGQARDAVLVDLALQGGGSHGAFTWGVLDRLMDETWLRIEAISGTSAGAMNAVVLAGGF 68 + G+A + +++ALQGGGSHGAF+WGVLDRL+++ L I A+SGTSAGAMNAV LA GF Sbjct: 3 KNGKAEENKKINIALQGGGSHGAFSWGVLDRLLEDGRLEISAVSGTSAGAMNAVALADGF 62 Query: 69 MTNGRAGAKSALEAFWRRVADSARFSLIRRSPLDVILGRWTLDTSPFYLAFDLASRVFSP 128 + G GA+ L+ FWR VA RFS ++R P DV G W+++ +P Y+ FD SRVFSP Sbjct: 63 VRGGVDGARQKLDDFWRAVAAKGRFSPVQRMPWDVAWGNWSIENTPGYVFFDTMSRVFSP 122 Query: 129 YDLNLAGTNPLHQVLAESVDFRQI-ANSPIRLFVTATNVHTGRGRVFRNAELTPDVLLAS 187 Y N G NPL V+A+ +DF+ + A + LF++ATNV TG+ RVF + E+ D ++AS Sbjct: 123 YVANPLGLNPLRDVVAQEIDFKNVRACKSMELFISATNVETGQLRVFSDGEIDLDTVMAS 182 Query: 188 ACLPTLFRAIEINGEPYWDGGYSGNPTITPLVRECDSHDTLLVAVNPVERPGTPRSAREI 247 ACLP LFRA+EI G PYWDGGY GNP + P + + D LLV +NPV R GTPRSA EI Sbjct: 183 ACLPQLFRAVEIKGVPYWDGGYGGNPALYPFFKTAATEDVLLVQINPVVREGTPRSANEI 242 Query: 248 LDRLNEIAFNATLLKELRMIALLRQVADAGSSEGRKWAEMRIHLI-ASKVLAGLGVSSKL 306 +R++EI FNA LL+E R IA ++++ AG ++ ++R+H I A + L SSK+ Sbjct: 243 QNRIDEITFNAGLLREFRSIAFVKELIAAGRLPHGEYRDIRMHRIDADEAFKDLSASSKV 302 Query: 307 NAEWDFLCMLRDEGRRAAEAFLVANDQNIGRRSSMDL 343 NAEW FL LRD GR AA +L N +G+R ++DL Sbjct: 303 NAEWAFLTYLRDLGRTAASDWLEENYDAVGKRPTLDL 339 >CC3383 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Ccr Caulobacter vibrioides Length = 353 Score = 278 bits (710), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 154/342 (45%), Positives = 213/342 (62%), Gaps = 22/342 (6%) Query: 19 VDLALQGGGSHGAFTWGVLDRLMDETWLRIEAISGTSAGAMNAVVLAGGFMTNGRAGAKS 78 + LALQGGG+HGAF WGVLDRL++E L I A++ SAGAMNAV LA G + GRAGA++ Sbjct: 16 LSLALQGGGAHGAFEWGVLDRLLEEDDLEIRAVTAASAGAMNAVCLAQGLIDEGRAGARA 75 Query: 79 ALEAFWRRVADSARFSLIRRSPLDVILGRWT---------LDTSPFYLAFDLASRVFSPY 129 LE+FWR V + ++ + G WT L +P + + + SPY Sbjct: 76 RLESFWRNVNRAGGRNVFGDT------GIWTNAFGGGVDWLKNTPGWRMAETLALSLSPY 129 Query: 130 DLNLAGTNPLHQVLAESVDFRQIAN-SPIRLFVTATNVHTGRGRVFRNAELTPDVLLASA 188 + N NPLH VL + F +I + SPI+L+++AT + T + +VFR ELT ++ASA Sbjct: 130 EFNPFNLNPLHDVLEGEITFSRIRDASPIKLYISATQIRTSKSKVFRETELTSRHIMASA 189 Query: 189 CLPTLFRAIEINGEPYWDGGYSGNPTITPLVRECDSHDTLLVAVNPVERPGTPRSAREIL 248 CLP LF+A+EI+GEPYWDGGY NP + PL + D L+V +NP R TP+S EI+ Sbjct: 190 CLPQLFQAVEIDGEPYWDGGYLANPPLWPLFYDDTPDDILIVTLNPFVREETPKSPGEIM 249 Query: 249 DRLNEIAFNATLLKELRMIALLRQVADAG----SSEGRKWAEMRIHLI-ASKVLAGLGVS 303 DRLNEI FNA+L+ ELR I ++++ D G ++ GR + M +H I A K LA L +S Sbjct: 250 DRLNEITFNASLVSELRAIGFVQKLLDEGLLKDNARGR-YRRMLVHAITADKPLADLSMS 308 Query: 304 SKLNAEWDFLCMLRDEGRRAAEAFLVANDQNIGRRSSMDLDL 345 +K N EW FL L+D GR AA+ +L +G +SS+DL + Sbjct: 309 TKFNTEWSFLTDLKDRGRAAAQVWLDDCGDCVGVKSSVDLKV 350 >RSc2551 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Rso Ralstonia solanacearum Length = 349 Score = 237 bits (604), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 138/337 (40%), Positives = 202/337 (59%), Gaps = 18/337 (5%) Query: 19 VDLALQGGGSHGAFTWGVLDRLMDETWLRIEAISGTSAGAMNAVVLAGGFMTNGRAGAKS 78 ++LALQGGG+HGAFTWGVLD L+++ L E +SGTSAG+MNAVV+ G + G A+ Sbjct: 9 INLALQGGGAHGAFTWGVLDALLEDGRLSFEGLSGTSAGSMNAVVMLDGLLEGGPERARE 68 Query: 79 ALEAFWRRVADSAR-FSLIRRSPLDVILGRWTLDTSPFYLAFDLASRVFSPYDLNLAGT- 136 L AFW V+ + FS + + ++ GR PF+ + +D ++ T Sbjct: 69 KLHAFWLAVSTAGSPFSTMGENAKTLLSGR------PFFPDSWPVADWMRMWDSWISATT 122 Query: 137 -----NPLHQVLAESVDFRQIANSP-IRLFVTATNVHTGRGRVFRNAELTPDVLLASACL 190 NPL +LA VDF ++ P +LF+ AT+V+TG RVF E+ +V+LASACL Sbjct: 123 QGAHYNPLRDLLANQVDFDRLRACPDTKLFLAATDVNTGNVRVFGTPEIDVEVVLASACL 182 Query: 191 PTLFRAIEINGEPYWDGGYSGNPTITPLVRECDSHDTLLVAVNPVERPGTPRSAREILDR 250 P L+ AIEI+ YWDGGY GNP + P E + D LLV VNP+ R P E+++R Sbjct: 183 PYLYPAIEIDRHHYWDGGYMGNPVLFPFFYETKTQDILLVHVNPIVRKDVPDQPHEVMER 242 Query: 251 LNEIAFNATLLKELRMIALLRQV--ADAGSSEGR-KWAEMRIHLI-ASKVLAGLGVSSKL 306 LNEI FNA+LL+E+R IA ++++ D E R + + +H I A + L L S+KL Sbjct: 243 LNEITFNASLLREMRAIAFVQKLIAEDWLKPEYRDRLKYIYLHAIRADRPLGDLSSSTKL 302 Query: 307 NAEWDFLCMLRDEGRRAAEAFLVANDQNIGRRSSMDL 343 +W FL ML++ GR+ A+ +L + ++GR ++D+ Sbjct: 303 VTDWSFLTMLKERGRQEAKLWLRKHFDDVGRNGTVDI 339 >slr0060 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Syn Synechocystis Length = 369 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 83/289 (28%), Positives = 127/289 (43%), Gaps = 35/289 (12%) Query: 19 VDLALQGGGSHGAFTWGVLDRLMD---ETWLRIEAISGTSAGAMNAVVLAGGFMTNGRAG 75 V +A QGGGS AFT GV+ L + + I ++SGTS GA+ A+++ + G Sbjct: 5 VAIACQGGGSQTAFTAGVMKALFENKMQDHFDIVSLSGTSGGAICALLIWYA-LKKGENP 63 Query: 76 AKSALEAFWRRVADSARFSLIRRSPLDVILGRWTLDTSPFYLAFDLASRVFSPYDLN-LA 134 L FW D++ + R D ++ L + + + +SP LA Sbjct: 64 VWKRLLDFW---LDNSAQTYQERFFNDFVIRSLELTSKGIVPQYSVTP--YSPLAQTWLA 118 Query: 135 GTNP--------LHQVLAESVDFRQIAN-----SPIRLFVTATNVHTGRGRVF--RNAEL 179 L ++L VDF ++A P L + A N+ TG+ F R + Sbjct: 119 SATQGLRSRFINLEELLQVHVDFSELATWGKQLEPPVLLIGACNILTGKLDKFNSRQEAI 178 Query: 180 TPDVLLASACLPTLFRAIEINGE----PYWDGGYSGNPTITPLVR------ECDSHDTLL 229 + LLASAC+P +F + I E YWDG +S NP I L R E + + Sbjct: 179 RIEHLLASACVPNIFPTVVIEEEGEKKAYWDGLFSDNPPIRSLYRQDFVGIENIPQEIWV 238 Query: 230 VAVNPVERPGTPRSAREILDRLNEIAFNATLLKELRMIALLRQVADAGS 278 + +NP + P A +I DR NE+ N +L + L I L + G+ Sbjct: 239 IKINPTQTDKIPTDADDIADRRNELEGNVSLFQSLDQIEFLNYLFIKGA 287 >mlr2398 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Mlo Mesorhizobium loti Length = 390 Score = 84.7 bits (208), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 84/258 (32%), Positives = 118/258 (45%), Gaps = 25/258 (9%) Query: 19 VDLALQGGGSHGAFTWGVLDRLMDETWLRIEAISGTSAGAMNAVVLAGGFMTNGRAGAKS 78 + L LQGGG+ GA+ GV + L E + + ++G S GA+N+ ++AG N Sbjct: 21 IALVLQGGGALGAYQAGVYEALA-EADIHPDWVAGVSIGAINSAIIAG----NEPTERVP 75 Query: 79 ALEAFWRRVADSARFSLIRRSPLDVILGRWT-LDTSPFYLAFDL---ASRVFS-----PY 129 L AFWR ++ + SP G L + F A L A FS P+ Sbjct: 76 KLRAFWREISANPLLDWAAASPALAPTGTLPRLLFNQFSAACALVAGAPGFFSLRQPVPW 135 Query: 130 ---DLNLAGTN-----PLHQVLAESVDFRQIANSPIRLFVTATNVHTGRGRVFRNAELT- 180 D +L GT+ L L VDF +I +R V A NV +G F N + T Sbjct: 136 LQADGSLEGTSFYETKHLKSTLERLVDFDRINAGRMRFSVGAVNVRSGNFVYFDNDQHTI 195 Query: 181 -PDVLLASACLPTLFRAIEINGEPYWDGGYSGNPTITPLVRECDSHDTLLVAVNPVERPG 239 P+ ++AS LP F A+EI+GE YWDGG N + +V DTL V+ G Sbjct: 196 RPEHVMASGSLPPGFPAVEIDGEFYWDGGLISNTPLQWVVEHGARQDTLAFQVDLWSARG 255 Query: 240 T-PRSAREILDRLNEIAF 256 PR+ E+ R EI + Sbjct: 256 EFPRNLSEVATRQKEIQY 273