BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0214.0010.p (334 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG2617G [ER] COG1063 Threonine dehydrogenase and related Zn-dep... 114 2e-25 mlr8299 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 113 5e-25 NMB1395 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 109 8e-24 SMc01564 [ER] COG1063 Threonine dehydrogenase and related Zn-dep... 109 8e-24 SMa1417 [ER] COG1063 Threonine dehydrogenase and related Zn-depe... 103 4e-22 >VNG2617G [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Hbs Halobacterium sp. NRC-1 Length = 347 Score = 114 bits (286), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 112/350 (32%), Positives = 159/350 (45%), Gaps = 40/350 (11%) Query: 11 MQAAVFHGNDRITIKRVAMPDVGTGE-VLVRVSRTALCGSDFKLWHKGAEFTAG----HE 65 M+AAV+ G I ++ V PD+ + E ++RV+ TA+CGSD + ++ AG HE Sbjct: 1 MRAAVYQGPGEIAVEEVPKPDIESPEDAVIRVTHTAVCGSDLWFYRGDSDREAGSRVGHE 60 Query: 66 IFGVVEQPGHRLH----GRRCAVYIPLHCGHCAACKRGDTQMCLEVSSLIGWNRPGGYAE 121 G+VE+ G + G R + CG C C++G C+E S GG E Sbjct: 61 PMGIVEEVGDDVTSVAPGDRVIAPFAISCGECEFCRQGLYTSCVEDESWGSEANGGGQGE 120 Query: 122 YVPVP--ENCLLPVPD---DIEDSLAPL--LLDTIGTSGHAVRFVSRVVPPGEAGPVLVM 174 YV P + L+ VPD D ED L L L D +GT HA VS V GE +V+ Sbjct: 121 YVKCPFADGTLVRVPDRYADDEDVLESLLPLTDVMGTGHHAA--VSAGV--GEGDTAVVV 176 Query: 175 GAGPVGLGVVLALRALGYNDIHVADPNATRLKIAQSFGAKA--HPVGDTSQRFAL----- 227 G G VGL VLA + LG I + RL++A FGA GD + A Sbjct: 177 GDGAVGLCGVLAAQRLGAERIIAMGHHEDRLELAAEFGATDTISARGDDAIERARDLTHG 236 Query: 228 ----IMECSGAHAARNLGIELVLPRGALVLVGENAAPWTIEEG--KVFRR-KDFYMIRTF 280 +MEC GA +A + I + P G + VG P+ +E+G VF D IR Sbjct: 237 GANHVMECVGAASAMDSAIAIARPGGTVGYVG---VPYGVEDGGLDVFTMFSDNITIRGG 293 Query: 281 YFPVSDFEPNI--ELLRKFKDEYRVLVDGEFGLSALPQNFARFARGELIK 328 PV + + ++L+ D + L +P+ +A E IK Sbjct: 294 VAPVRAYAEELMADVLQGTLDPSPIFTK-TVDLDGVPEGYAAMDDREAIK 342 >mlr8299 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Mlo Mesorhizobium loti Length = 344 Score = 113 bits (283), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 71/235 (30%), Positives = 123/235 (52%), Gaps = 20/235 (8%) Query: 22 ITIKRVAMPDVGTGEVLVRVSRTALCGSDFKL--WHKGAEFT------AGHEIFGVVEQP 73 I ++ V +P++G +VL+++ +TA+CG+D + W + A+ T GHE G V Sbjct: 17 IWMEEVPVPEIGPNDVLIKIRKTAICGTDVHIYNWDQWAQKTVPVPMVTGHEFVGTVADF 76 Query: 74 GHRLH----GRRCAVYIPLHCGHCAACKRGDTQMCLEVSSLIGWNRPGGYAEYVPVPENC 129 G + G+R + + CGHC C+ G +C +G NRPG + EY+ +P++ Sbjct: 77 GAAVTEYKVGQRVSGEGHIVCGHCRNCRAGRGHLCRNTLG-VGVNRPGAFGEYLAIPQHN 135 Query: 130 LLPVPDDIEDSLAPLLLDTIGTSGHAVRFVSRVVPPGEAGPVLVMGAGPVGLGVVLALRA 189 ++P+PDD+ D +A + D +G + H V GE VLV GAGP+G+ L + Sbjct: 136 VVPIPDDVPDEIAA-IFDPLGNAVHTALSFDLV---GE--DVLVTGAGPIGIMGALVAQC 189 Query: 190 LGYNDIHVADPNATRLKIAQSFGAKAHPVGDTSQRFALIMECSGAHAARNLGIEL 244 +G + + D N RL +A+ G + H V + ++ +M G ++G+E+ Sbjct: 190 VGARKVVITDINPVRLALAKKLGVQ-HVVDASKEKLRDVMPVLGMTEGFDVGLEM 243 >NMB1395 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Nme Neisseria meningitidis MC58 Length = 346 Score = 109 bits (273), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 94/349 (26%), Positives = 161/349 (46%), Gaps = 34/349 (9%) Query: 11 MQAAVFHGNDRITIKRVAMPDV-GTGEVLVRVSRTALCGSDFKLWH-KGAEFTAG----H 64 M+A V+HG + I + P + + +V++ +T +CG+D +W K E G H Sbjct: 1 MKAMVYHGANDIRFEEKPRPQIIDPTDAVVKIVKTTICGTDLGIWKGKNPEVADGRILGH 60 Query: 65 EIFGVVEQPGHRLHGRRCAVYIPLHC-GHCAACKRGDTQM---CLEVSSLIGWNRPGGYA 120 E G+VE+ G + + + + C C C TQ+ C ++G+ G A Sbjct: 61 EGIGIVEEVGEAVKNIKVGDKVIISCVSKCCTCDNCKTQLYSHCRNGGWILGYMIDGTQA 120 Query: 121 EYV--PVPENCLLPVPDDIEDSLAPLLLDTIGTSGHAVRFVSRVVPPGEAGPVLVMGAGP 178 EYV P +N L+P+PD++ + +A LL D + T+ H + V PG+ V + GAGP Sbjct: 121 EYVRTPYADNSLVPLPDNVNEEIALLLSDALPTA-HEIGVQYGDVKPGDT--VFIAGAGP 177 Query: 179 VGLGVVLALRALGYNDIHVADPNATRLKIAQSFGAKAHPVGDTS-----QRFALI----- 228 VG+ +L + I V D + RLK+A+ GA H + S Q FA++ Sbjct: 178 VGMSALLTAQLYSPAAIIVCDMDENRLKLAKELGA-THTINPASGEVSKQVFAIVGEDGV 236 Query: 229 ---MECSGAHAARNLGIELVLPRGALVLVGENAAPWTIEEGKVFRRKDFYMIRTFYFPVS 285 +E G A N+ ++V P G + +VG + + K++ +K I T + Sbjct: 237 DCAIEAVGIPATWNMCQDIVKPGGHIAVVGVHGQSVDFKLEKLWIKK--LAITTGLVNAN 294 Query: 286 DFEPNIELLRKFKDEYRVLVDGEFGLSALPQNFARF---ARGELIKPVL 331 E ++ + +Y ++ F S L + + F A +++K VL Sbjct: 295 TTEMLMKAISSSSVDYTKMLTHHFKFSELEKAYDVFKHAAENQVMKVVL 343 >SMc01564 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sme Sinorhizobium meliloti Length = 344 Score = 109 bits (273), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 73/235 (31%), Positives = 121/235 (51%), Gaps = 20/235 (8%) Query: 22 ITIKRVAMPDVGTGEVLVRVSRTALCGSDFKLWH--KGAEFT------AGHEIFGVVEQP 73 + ++RV +P++G +VL+RV ++A+CG+D +W+ + AE T GHE G V + Sbjct: 17 LWMERVPVPEIGPNDVLIRVRKSAICGTDVHIWNWDQWAEKTIPVPMVVGHEFMGEVVEV 76 Query: 74 GHRLH----GRRCAVYIPLHCGHCAACKRGDTQMCLEVSSLIGWNRPGGYAEYVPVPENC 129 G + G R + + CG C C+ G +C +G NRPG +AE+V +PE Sbjct: 77 GPAVSKHHVGERVSGEGHIVCGKCRNCRAGRGHLCRNTLG-VGVNRPGSFAEFVCLPEYN 135 Query: 130 LLPVPDDIEDSLAPLLLDTIGTSGHAVRFVSRVVPPGEAGPVLVMGAGPVGLGVVLALRA 189 ++ +PDD+ D +A + D G + H V GE VLV GAGP+G+ + + Sbjct: 136 VVSIPDDVPDEIAA-IFDPFGNAVHTALSFDLV---GE--DVLVTGAGPIGIMGAMVAKR 189 Query: 190 LGYNDIHVADPNATRLKIAQSFGAKAHPVGDTSQRFALIMECSGAHAARNLGIEL 244 G + + D N RL +A+ G H V + ++ A +M G ++G+E+ Sbjct: 190 CGARKVVITDINPVRLDLARKLGID-HVVDASKEKLADVMRVIGMTEGFDVGLEM 243 >SMa1417 [ER] COG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenases Sme Sinorhizobium meliloti Length = 341 Score = 103 bits (258), Expect = 4e-22, Method: Compositional matrix adjust. Identities = 75/236 (31%), Positives = 122/236 (51%), Gaps = 16/236 (6%) Query: 11 MQAAVFHGNDRITIKRVAMPDVGTGEVLVRVSRTALCGSDFKLW--HKGAE---FTAGHE 65 M+AAV R ++ V +P++G +VL+RV+R +CG+D ++ H A+ GHE Sbjct: 1 MKAAVLVEPRRFEVREVGIPEIGPADVLIRVTRAGICGTDLHIFNGHYAADRLPIVPGHE 60 Query: 66 IFGVVEQPG----HRLHGRRCAVYIPLHCGHCAACKRGDTQMCLEVSSLIGWNRPGGYAE 121 G + + G H G R I + CG+C C+R + C EV IG R G +A+ Sbjct: 61 FCGTIAEIGASVTHLKTGMRVVADINIGCGNCYWCRRNEVLNCGEVEQ-IGIGRDGAFAQ 119 Query: 122 YVPVPENCLLPVPDDIEDSLAPLLLDTIGTSGHAVRFVSRVVPPGEAGPVLVMGAGPVGL 181 YV +P +LPVPD + +++ L++ + A R G +G +V+GAGP+G Sbjct: 120 YVALPGRLVLPVPDGVPEAVLA-LVEPVACVVRAARKAGAAF--GRSG--VVLGAGPIGN 174 Query: 182 GVVLALRALGYNDIHVADPNATRLKIAQSFGAKAHPVGDTSQRFALIMECSGAHAA 237 V +R +G I VAD ++ R ++A GA A V + + + ++E +G A Sbjct: 175 LHVQMMRLVGMAPIIVADLSSERCRMAVEAGADA-AVSEPATLRSKVLEMTGGRGA 229