BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0209.0013.p (532 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2006 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 287 4e-77 PA2557 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 261 2e-69 MT0175 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 261 4e-69 Rv0166 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 261 4e-69 Rv2505c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 257 4e-68 >BH2006 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bha Bacillus halodurans Length = 513 Score = 287 bits (735), Expect = 4e-77, Method: Compositional matrix adjust. Identities = 179/508 (35%), Positives = 275/508 (54%), Gaps = 10/508 (1%) Query: 27 ERPGSLWAMIADAVAQNGDGEALVCGATRLSWREVAEKSAKVAAGFAKLGLTPGDRVAIL 86 E+ S++ ++ AV N EAL R+++ ++ E+ ++AA F ++G+ GDR+ + Sbjct: 3 EKDYSVFPLLYQAVQANPKKEALYDLNERITYEQLLERVNELAAIFIEMGVVKGDRIGVC 62 Query: 87 LGNRIEFVLTMFAAAHAGLVTVLLSTRQQKPEIAYVLNDCGATLLVHEAALADRIPDAAD 146 L N E V+ FAAA G V + ++ EIAY++ + LL + + + AA Sbjct: 63 LPNWNETVIIFFAAAKLGATVVPFNPNYREYEIAYIVANAAPKLLFVCEKVEENVGLAAL 122 Query: 147 IPGLKHRISVSDDG--ASQFAGLA-DNAPAQAPAQVREEDTAMILYTSGTTGRPKGAMLA 203 + + ISV A F L N A + V + ILYTSGTTG PKG M+ Sbjct: 123 VADQRQLISVRFKSPFAIPFEQLKRTNKSAIDVSIVPSHERYCILYTSGTTGLPKGVMIT 182 Query: 204 HCNIVHSSMVFASCLKLTRADRSIAAVPLAHVTGAVANITTMARCAGALIIMPEFKASEY 263 H ++V S + A LK T+ D I PL H+ G N+ C +I+ +FK Sbjct: 183 HSSVVQSGLALARSLKCTKDDVFIVPAPLFHIFGMACNLMAAVSCQAKVILQEKFKPDHT 242 Query: 264 LKVAARERVTYTVMVPAMYNLCLMQPDFDSYDLSSWRIGGFGGAPMPVATIEKLDAKIPG 323 L + +E+VT VP M+ L L PDF ++DLSS R G G AP P T++++ ++ G Sbjct: 243 LALIEQEKVTIHQAVPTMFILELNHPDFSTFDLSSLRAGMVGAAPCPKETVQEIRKRM-G 301 Query: 324 LKLANCYGATETTSPSTLMPGE-LTAAHIDSVGLPCPGAEIVVMGADGRELPRGEIGELW 382 L YG TE + +T+ P E + +D+VG P G EI ++ D LP G+IGE+ Sbjct: 302 FHLCISYGMTEVGA-ATITPYEDEDESSLDTVGKPMEGVEITIVNEDREPLPVGDIGEIA 360 Query: 383 IRSASVIKGYWNNPKATAESF-TAGFWHSGDLGSIDVKNFVRVFDRQKDMINRGGLKIYS 441 IR + GY+ P+ T E G++++GDLGS+D + ++R R+K++I RGG IY Sbjct: 361 IRGFGNMIGYYKLPEQTNEVLGDNGWFYTGDLGSLDEEGYLRFIGRKKELIIRGGYNIYP 420 Query: 442 AEVESVLAGHPAVVESAIIAKPCPVLGERVHAVIVTRTDVNA--ENLRAWCAERLSDYKV 499 E+E++L+ H V ESA+I P VLGE V A I + ++ + L A+ ++R++ YKV Sbjct: 421 QEIEAILSEHEKVQESAVIGLPDEVLGELVCAGIKLKQGAHSSEQELLAYLSKRIAHYKV 480 Query: 500 PETMALTSEPLPRNANGKVIKRQIRESL 527 P + E LP A+GKV K Q+RE + Sbjct: 481 PSKIVFVEE-LPVTASGKVQKSQLREQI 507 >PA2557 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Pae Pseudomonas aeruginosa Length = 564 Score = 261 bits (668), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 181/523 (34%), Positives = 259/523 (49%), Gaps = 39/523 (7%) Query: 40 VAQNGDGEALVCGATRL--SWREVAEKSAKVAAGFAKLGLTPGDRVAILLGNRIEFVLTM 97 VA++ + EALV RL SWR++AE+ A F LGL PG+R+ I N E+ +T Sbjct: 28 VARHAEREALVVRHQRLRYSWRQLAERVDAYARAFIALGLRPGERLGIWAPNCAEWCITQ 87 Query: 98 FAAAHAGLVTVLLSTRQQKPEIAYVLNDCGATLLV---------HEAALADRIPDAA--- 145 FA+A G V V ++ + E+ Y L G + L+ + A L D +P+ A Sbjct: 88 FASAKVGAVLVNINPAYRSSELEYALKQSGCSWLICADAFKTSDYHAMLGDLLPELARAR 147 Query: 146 -------DIPGLKHRISVSDDGASQF---AGLADNAPAQAPAQVREEDTAM-------IL 188 +P L+ IS+++ + F GL A A ++R+ ++ I Sbjct: 148 PGELASERLPELRGVISLAERAPAGFLHWQGLPGLAAAVGAEELRQRQASLQFDEPINIQ 207 Query: 189 YTSGTTGRPKGAMLAHCNIVHSSMVFASCLKLTRADRSIAAVPLAHVTGAV-ANITTMAR 247 YTSGTTG PKGA L+H NI+++ + L L DR + VPL H G V N+ + Sbjct: 208 YTSGTTGFPKGATLSHYNILNNGYMVGESLGLGAEDRLVIPVPLYHCFGMVMGNLGCVTH 267 Query: 248 CAGALIIMPEFKASEYLKVAARERVTYTVMVPAMYNLCLMQPDFDSYDLSSWRIGGFGGA 307 + + P F A L A ER T VP M+ L P +DLSS R G GA Sbjct: 268 GSTMIYPAPSFDAEATLLAVAEERATALYGVPTMFIAELDHPRRREFDLSSLRTGIMAGA 327 Query: 308 PMPVATIEKLDAKIPGLKLANCYGATETTSPSTLM--PGELTAAHIDSVGLPCPGAEIVV 365 P+ + ++ + ++ YG TE TSP +L P + + +VG P E + Sbjct: 328 TCPIEVMRRVIGDMHMAEVQIAYGMTE-TSPVSLQTGPDDGLELRVTTVGRTQPRLESKI 386 Query: 366 MGADGRELPRGEIGELWIRSASVIKGYWNNPKATAESFTAGFW-HSGDLGSIDVKNFVRV 424 + GR +PRGEIGEL R SV+ GYWN+P+ATAE+ W H+GDL +D +VR+ Sbjct: 387 VDQTGRVVPRGEIGELCTRGYSVMLGYWNDPQATAEAIDPARWMHTGDLAVMDDDGYVRI 446 Query: 425 FDRQKDMINRGGLKIYSAEVESVLAGHPAVVESAIIAKPCPVLGERVHAVIVTRTDVNA- 483 R KDMI RGG IY E+E HPAV + +I P GE + A I +A Sbjct: 447 VGRSKDMIIRGGENIYPRELEEFFFTHPAVADVQVIGIPDERYGEEIVAWIKLHPGHHAD 506 Query: 484 -ENLRAWCAERLSDYKVPETMALTSEPLPRNANGKVIKRQIRE 525 E LRA+C R++ +K+P E P GK+ K ++RE Sbjct: 507 DEQLRAFCKARIAHFKIPRHFKFVDE-FPMTVTGKIQKFRMRE 548 >MT0175 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II MtC Mycobacterium tuberculosis CDC1551 Length = 518 Score = 261 bits (666), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 172/493 (34%), Positives = 262/493 (53%), Gaps = 20/493 (4%) Query: 45 DGEALVCGATRLSWREVAEKSAKVAAGFAKLGLTPGDRVAILLGNRIEFVLTMFAAAHAG 104 D AL ++W ++ + A +A + G+ GDRV IL+ NR EFV ++ AA G Sbjct: 5 DAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAANMIG 64 Query: 105 LVTVLLSTRQQKPEIAYVLNDCGATLLVHEAALADRIPDAADIPGLKHRISV-------S 157 + V L+ R EIA ++ DC A +++ EAALA P A + ++ +SV S Sbjct: 65 AIAVPLNFRLTPTEIAVLVEDCVAHVMLTEAALA---PVAIGVRNIQPLLSVIVVAGGSS 121 Query: 158 DDGASQFAGLADNA-PAQAPAQVREEDTAMILYTSGTTGRPKGAMLAHCNIVHSSM--VF 214 D + L + A P + + A+I+YTSGTTGRPKGA+L H N+ +M ++ Sbjct: 122 QDSVFGYEDLLNEAGDVHEPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALY 181 Query: 215 ASCLKLTRADRSIAAVPLAHVTGAVANITTMARCAGALIIMP--EFKASEYLKVAARERV 272 S + +D VPL H+ G + N+ T +I P F + L V E+V Sbjct: 182 TSGANIN-SDVGFVGVPLFHIAG-IGNMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAEKV 239 Query: 273 TYTVMVPAMYNLCLMQPDFDSYDLSSWRIGGFGGAPMPVATIEKLDAKIPGLKLANCYGA 332 T +VPA + + DL R+ +G AP P A + ++ A P ++ +G Sbjct: 240 TGIFLVPAQWQAVCTEQQARPRDLR-LRVLSWGAAPAPDALLRQMSATFPETQILAAFGQ 298 Query: 333 TETTSPSTLMPGELTAAHIDSVGLPCPGAEIVVMGADGRELPRGEIGELWIRSASVIKGY 392 TE + + ++ GE A SVG P V+ + ++P GE+GE+ R+ +++ Y Sbjct: 299 TEMSPVTCMLLGEDAIAKRGSVGRVIPTVAARVVDQNMNDVPVGEVGEIVYRAPTLMSCY 358 Query: 393 WNNPKATAESFTAGFWHSGDLGSIDVKNFVRVFDRQKDMINRGGLKIYSAEVESVLAGHP 452 WNNP+ATAE+F G++HSGDL +D +V V DR+KDMI GG IY AE+E+VLA HP Sbjct: 359 WNNPEATAEAFAGGWFHSGDLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHP 418 Query: 453 AVVESAIIAKPCPVLGERVHAV-IVTRTDVNAENLRAWCAERLSDYKVPETMALTSEPLP 511 + E A+I + GE AV VT D+ E+L + +RL+ YK P+ + + + LP Sbjct: 419 DIAEVAVIGRADEKWGEVPIAVAAVTNDDLRIEDLGEFLTDRLARYKHPKALEIV-DALP 477 Query: 512 RNANGKVIKRQIR 524 RN GKV+K ++R Sbjct: 478 RNPAGKVLKTELR 490 >Rv0166 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 554 Score = 261 bits (666), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 172/493 (34%), Positives = 262/493 (53%), Gaps = 20/493 (4%) Query: 45 DGEALVCGATRLSWREVAEKSAKVAAGFAKLGLTPGDRVAILLGNRIEFVLTMFAAAHAG 104 D AL ++W ++ + A +A + G+ GDRV IL+ NR EFV ++ AA G Sbjct: 41 DAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVMILMLNRTEFVESVLAANMIG 100 Query: 105 LVTVLLSTRQQKPEIAYVLNDCGATLLVHEAALADRIPDAADIPGLKHRISV-------S 157 + V L+ R EIA ++ DC A +++ EAALA P A + ++ +SV S Sbjct: 101 AIAVPLNFRLTPTEIAVLVEDCVAHVMLTEAALA---PVAIGVRNIQPLLSVIVVAGGSS 157 Query: 158 DDGASQFAGLADNA-PAQAPAQVREEDTAMILYTSGTTGRPKGAMLAHCNIVHSSM--VF 214 D + L + A P + + A+I+YTSGTTGRPKGA+L H N+ +M ++ Sbjct: 158 QDSVFGYEDLLNEAGDVHEPVDIPNDSPALIMYTSGTTGRPKGAVLTHANLTGQAMTALY 217 Query: 215 ASCLKLTRADRSIAAVPLAHVTGAVANITTMARCAGALIIMP--EFKASEYLKVAARERV 272 S + +D VPL H+ G + N+ T +I P F + L V E+V Sbjct: 218 TSGANIN-SDVGFVGVPLFHIAG-IGNMLTGLLLGLPTVIYPLGAFDPGQLLDVLEAEKV 275 Query: 273 TYTVMVPAMYNLCLMQPDFDSYDLSSWRIGGFGGAPMPVATIEKLDAKIPGLKLANCYGA 332 T +VPA + + DL R+ +G AP P A + ++ A P ++ +G Sbjct: 276 TGIFLVPAQWQAVCTEQQARPRDLR-LRVLSWGAAPAPDALLRQMSATFPETQILAAFGQ 334 Query: 333 TETTSPSTLMPGELTAAHIDSVGLPCPGAEIVVMGADGRELPRGEIGELWIRSASVIKGY 392 TE + + ++ GE A SVG P V+ + ++P GE+GE+ R+ +++ Y Sbjct: 335 TEMSPVTCMLLGEDAIAKRGSVGRVIPTVAARVVDQNMNDVPVGEVGEIVYRAPTLMSCY 394 Query: 393 WNNPKATAESFTAGFWHSGDLGSIDVKNFVRVFDRQKDMINRGGLKIYSAEVESVLAGHP 452 WNNP+ATAE+F G++HSGDL +D +V V DR+KDMI GG IY AE+E+VLA HP Sbjct: 395 WNNPEATAEAFAGGWFHSGDLVRMDSDGYVWVVDRKKDMIISGGENIYCAELENVLASHP 454 Query: 453 AVVESAIIAKPCPVLGERVHAV-IVTRTDVNAENLRAWCAERLSDYKVPETMALTSEPLP 511 + E A+I + GE AV VT D+ E+L + +RL+ YK P+ + + + LP Sbjct: 455 DIAEVAVIGRADEKWGEVPIAVAAVTNDDLRIEDLGEFLTDRLARYKHPKALEIV-DALP 513 Query: 512 RNANGKVIKRQIR 524 RN GKV+K ++R Sbjct: 514 RNPAGKVLKTELR 526 >Rv2505c [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 547 Score = 257 bits (657), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 176/521 (33%), Positives = 256/521 (49%), Gaps = 34/521 (6%) Query: 31 SLWAMIADAVAQNGDGEALV--CGATRLSWREVAEKSAKVAAGFAKLGLTPGDRVAILLG 88 ++ A +A A+ G EALV R ++ E+ ++A G + G+ PGDRV I Sbjct: 27 TIGANLAATAARYGHREALVDMVARRRFNYSELLTDVHRLATGLVRAGIGPGDRVGIWAP 86 Query: 89 NRIEFVLTMFAAAHAGLVTVLLSTRQQKPEIAYVLNDCGATLLVHEAALAD--------- 139 NR E+VL +A A G + V ++ + E+ Y L G +++ A+ D Sbjct: 87 NRWEWVLVQYATAEIGAILVTINPAYRVREVEYALRQSGVAMVIAVASFKDADYAAMLAE 146 Query: 140 ---RIPDAADIPGLKHRISVSDDGASQFAGLADNAPA--QAPAQVREEDTAMILYTSGTT 194 R PD AD+ I + D AG + PA Q A++ D I YTSGTT Sbjct: 147 VGPRCPDLADV------ILLESDRWDALAGAEPDLPALQQTAARLDGSDPVNIQYTSGTT 200 Query: 195 GRPKGAMLAHCNIVHSSMVFASCLKLTRADRSIAAVPLAHVTGAV-ANITTMARCAGALI 253 PKG L+H NI+++ + L T DR VP H G V N+ + A +I Sbjct: 201 AYPKGVTLSHRNILNNGYLVGELLGYTAQDRICIPVPFYHCFGMVMGNLAATSHGAAMVI 260 Query: 254 IMPEFKASEYLKVAARERVTYTVMVPAMYNLCLMQPDFDSYDLSSWRIGGFGGAPMPVAT 313 P F + L+ ER T VP M+ L PDF Y+L S R G GA PV Sbjct: 261 PAPGFDPAATLRAVQDERCTSLYGVPTMFIAELGLPDFTDYELGSLRTGIMAGAACPVEV 320 Query: 314 IEKLDAKI--PGLKLANCYGATETTSPSTLMPGELTA-AHIDSVGLPCPGAEI-VVMGAD 369 + K+ +++ PG+ + CYG TET+ ST + + + +VG P EI VV A Sbjct: 321 MRKVISRMHMPGVSI--CYGMTETSPVSTQTRADDSVDRRVGTVGRVGPHLEIKVVDPAT 378 Query: 370 GRELPRGEIGELWIRSASVIKGYWNNPKATAESFTAGFW-HSGDLGSIDVKNFVRVFDRQ 428 G +PRG +GE R SV+ GYWN+P+ TAE A W H+GDL +D +VR+ R Sbjct: 379 GETVPRGVVGEFCTRGYSVMAGYWNDPQKTAEVIDADGWMHTGDLAEMDPSGYVRIAGRI 438 Query: 429 KDMINRGGLKIYSAEVESVLAGHPAVVESAIIAKPCPVLGERVHAVIVTRTD---VNAEN 485 KD++ RGG I E+E +L HP +V+ +I P GE + AV+ R D + E Sbjct: 439 KDLVVRGGENISPREIEELLHTHPDIVDGHVIGVPDAKYGEELMAVVKLRNDAPELTIER 498 Query: 486 LRAWCAERLSDYKVPETMALTSEPLPRNANGKVIKRQIRES 526 LR +C R++ +K+P + + E P GKV K ++R+ Sbjct: 499 LREYCMGRIARFKIPRYLWIVDE-FPMTVTGKVRKVEMRQQ 538