BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0203.0037.p (229 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll8140 [K] COG1595 DNA-directed RNA polymerase specialized sigm... 273 3e-73 SMb20592 [K] COG1595 DNA-directed RNA polymerase specialized sig... 257 1e-68 BH0263 [K] COG1595 DNA-directed RNA polymerase specialized sigma... 108 8e-24 BS_sigW [K] COG1595 DNA-directed RNA polymerase specialized sigm... 99 9e-21 RSc1055 [K] COG1595 DNA-directed RNA polymerase specialized sigm... 97 3e-20 >mll8140 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Mlo Mesorhizobium loti Length = 208 Score = 273 bits (697), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 129/207 (62%), Positives = 173/207 (83%), Gaps = 4/207 (1%) Query: 19 LVQRALARDEAAIRAIMQANNRRLFRLARGILRNDHEAEDVVQDAYVRAFTHLDQFRGES 78 LV+RALARD A R I++ +N+RL+RLARGILRND EAEDVVQ+AYVRAF +L FRG++ Sbjct: 3 LVRRALARDAGAFRTIIKTHNQRLYRLARGILRNDAEAEDVVQEAYVRAFANLAAFRGDA 62 Query: 79 SLSTWLSRIAMNEALGRLRRRHSSVEWSDLPKGAPEAQIIQFPLSSTDDPEKSMAQREIQ 138 SL+TWLSRI +NEALGRLR+R +V + +PEAQ+I+FPL+ +DDPE++MAQR+I Sbjct: 63 SLATWLSRIVINEALGRLRKRRRTVAMPE----SPEAQVIRFPLNPSDDPERTMAQRQIL 118 Query: 139 HVVEHAIDELPEAFRLVFITRVIEGMNVEETAEILGLKPETVKTRLHRARTMLRDNVERK 198 +VE A D LP+ +R+VF+ RVIEG+++EETA++LG++PETVKTRLHRAR +LR ++ + Sbjct: 119 ALVERATDSLPDIYRMVFVARVIEGLSMEETADLLGVRPETVKTRLHRARALLRKALDDE 178 Query: 199 IGPVVMEAFPFAGKRCERLTEAVLKRL 225 IGPV+++AFPFAG+RC RLT AV+ RL Sbjct: 179 IGPVLLDAFPFAGRRCGRLTRAVMGRL 205 >SMb20592 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Sme Sinorhizobium meliloti Length = 227 Score = 257 bits (657), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 125/216 (57%), Positives = 169/216 (78%), Gaps = 1/216 (0%) Query: 11 PTSSGDAELVQRALARDEAAIRAIMQANNRRLFRLARGILRNDHEAEDVVQDAYVRAFTH 70 PT+ D L LA+D A RAIMQA+NR+L+R+AR ILRND +AEDV+Q+AYVRAFT Sbjct: 2 PTAETDLCLNAPPLAQDLARFRAIMQAHNRKLYRIARSILRNDSDAEDVLQEAYVRAFTR 61 Query: 71 LDQFRGESSLSTWLSRIAMNEALGRLRRRHSSVEWSDLPKGAPEAQIIQFPLSS-TDDPE 129 + FRG+++++TWL+RI +NEALGRLR+ + + + + EA+II FPL+S DPE Sbjct: 62 FEDFRGDAAITTWLARIVINEALGRLRKGRRQMRLAKYMRQSQEAEIIAFPLNSGISDPE 121 Query: 130 KSMAQREIQHVVEHAIDELPEAFRLVFITRVIEGMNVEETAEILGLKPETVKTRLHRART 189 K+MAQR+I +VE D+LP+ +R VF+ RVIEG++ EE A +LGLKPET +TRLHRAR Sbjct: 122 KTMAQRQILDLVERLTDDLPDTYRTVFVLRVIEGLDNEEAAALLGLKPETARTRLHRARR 181 Query: 190 MLRDNVERKIGPVVMEAFPFAGKRCERLTEAVLKRL 225 +L++ +ER+IGPV+++AFPFAGKRCERLTEAVL R+ Sbjct: 182 ILKEQLERQIGPVLLDAFPFAGKRCERLTEAVLARI 217 >BH0263 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Bha Bacillus halodurans Length = 187 Score = 108 bits (271), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 61/181 (33%), Positives = 107/181 (59%), Gaps = 2/181 (1%) Query: 13 SSGDAELVQRALARDEAAIRAIMQANNRRLFRLARGILRNDHEAEDVVQDAYVRAFTHLD 72 S D LV+ A DE A +++ +++++A ++ N HEA+DV Q+A++RA+T++D Sbjct: 2 DSIDKRLVREVRAGDEQAFAELVELYKDKVYQVAYRMVGNVHEAQDVAQEAFLRAYTNID 61 Query: 73 QFRGESSLSTWLSRIAMNEALGRLRRRHSSVEWSDLPKGAPEAQIIQFPLSSTDDP-EKS 131 + + STWL RIA N A+ RLR+R D +GA + I+ L++ ++P + Sbjct: 62 TYDIQRKFSTWLFRIATNVAIDRLRKRKPDFHLEDPVQGA-DGLTIESQLAADEEPVDDQ 120 Query: 132 MAQREIQHVVEHAIDELPEAFRLVFITRVIEGMNVEETAEILGLKPETVKTRLHRARTML 191 + Q E + ++ I LP +R+ + + +E ++++E ++IL L TVKTR+HR R L Sbjct: 121 VIQMETRDWIQQEIQALPPKYRMAVLLKYMEDLSLKEISDILDLPVSTVKTRIHRGREAL 180 Query: 192 R 192 R Sbjct: 181 R 181 >BS_sigW [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Bsu Bacillus subtilis Length = 187 Score = 98.6 bits (244), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 51/166 (30%), Positives = 89/166 (53%) Query: 27 DEAAIRAIMQANNRRLFRLARGILRNDHEAEDVVQDAYVRAFTHLDQFRGESSLSTWLSR 86 D+ A I+ ++++L +L N HEAED+ Q+A++RA+ ++D F STWL R Sbjct: 16 DQDAFADIVDIYKDKIYQLCYRMLGNVHEAEDIAQEAFIRAYVNIDSFDINRKFSTWLYR 75 Query: 87 IAMNEALGRLRRRHSSVEWSDLPKGAPEAQIIQFPLSSTDDPEKSMAQREIQHVVEHAID 146 IA N + R+R++ G + ++ PE ++ E+ + ++ I Sbjct: 76 IATNLTIDRIRKKKPDYYLDAEVAGTEGLTMYSQIVADGVLPEDAVVSLELSNTIQQKIL 135 Query: 147 ELPEAFRLVFITRVIEGMNVEETAEILGLKPETVKTRLHRARTMLR 192 +LP+ +R V + + I+ +++ E EIL + TVKTR+HR R LR Sbjct: 136 KLPDKYRTVIVLKYIDELSLIEIGEILNIPVGTVKTRIHRGREALR 181 >RSc1055 [K] COG1595 DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog Rso Ralstonia solanacearum Length = 199 Score = 97.1 bits (240), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 63/190 (33%), Positives = 99/190 (52%), Gaps = 7/190 (3%) Query: 15 GDAELVQRALARDEAAIRAIMQANNRRLFRLARGILRNDHEAEDVVQDAYVRAFTHLDQF 74 D LV+R D+ A ++ +R++ RL ++R+ E EDV QDA+++A+ L QF Sbjct: 6 ADQILVERVQQGDKRAFELLVVKYHRKIIRLISRLIRDAAEVEDVAQDAFIKAYRALPQF 65 Query: 75 RGESSLSTWLSRIAMNEALGRL----RRRHSSVEWSDLPKGAPEAQIIQFPLSSTDDPEK 130 RGES+ TWL RIA+N A L RR SS +D+ E L + P+ Sbjct: 66 RGESAFYTWLYRIAVNTAKNYLATQGRRPESS---TDIDTEEAETFADGEQLRDINTPDS 122 Query: 131 SMAQREIQHVVEHAIDELPEAFRLVFITRVIEGMNVEETAEILGLKPETVKTRLHRARTM 190 + +++ V A++ LPE R R IEG++ EE AE + TV++R+ RAR Sbjct: 123 MLHTKQVAETVNRAMEALPEELRTAITLREIEGLSYEEIAEAMECPIGTVRSRIFRAREA 182 Query: 191 LRDNVERKIG 200 + + + +G Sbjct: 183 IAEKLRPLLG 192