BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0172.p (369 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00770 [E] COG0687 Spermidine/putrescine-binding periplasmic p... 471 e-132 mlr6533 [E] COG0687 Spermidine/putrescine-binding periplasmic pr... 470 e-132 BMEI0411 [E] COG0687 Spermidine/putrescine-binding periplasmic p... 458 e-129 AGc1084 [E] COG0687 Spermidine/putrescine-binding periplasmic pr... 444 e-124 mlr6992 [E] COG0687 Spermidine/putrescine-binding periplasmic pr... 434 e-121 >SMc00770 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Sme Sinorhizobium meliloti Length = 364 Score = 471 bits (1212), Expect = e-132, Method: Compositional matrix adjust. Identities = 221/365 (60%), Positives = 285/365 (78%), Gaps = 4/365 (1%) Query: 8 LVSFVGAVATVLLLSAS---ADAEERTVNFYNWSNYMAPEVLEDFTKETGIKVVYDTFDA 64 + + A T +L+ S A A+ER VN YNWS+Y+ +LE+FTKETGIKVVYD FD+ Sbjct: 1 MSKLIVATLTTAVLAGSTILAFAQERVVNVYNWSDYIDDSILEEFTKETGIKVVYDVFDS 60 Query: 65 NETLETRLLAGKSGYDVVVPTGYFLQRQITAKVFLKLDKSKLPNLANAWPVVTSQLATYD 124 NE LET+LLAG SGYDVVVPT YFLQRQI A VF KLDKSKLPN++N W +V + A YD Sbjct: 61 NEILETKLLAGGSGYDVVVPTAYFLQRQIAAGVFQKLDKSKLPNISNMWDMVMERTAQYD 120 Query: 125 PGNNYGANYMWGTTGIGYNVKIAEKILGPDAKIDSWDMVFKPENLAKFKDCGIHMLDSAD 184 PGN Y +YMWGTTGIGYNV+ ++ILG D K +WD++F PE AKFKDCGIH+LDS Sbjct: 121 PGNEYAVDYMWGTTGIGYNVEKMKEILGTDEK-PNWDVIFDPEIAAKFKDCGIHLLDSPT 179 Query: 185 DILPAALSYLGLDPNSTKQADLEKAADLVIRIRPYVRKFHSSEYLSALASGEICFVVGWS 244 DI+P+AL+YLGL+P+S QADLEKAADL++++RP +RKFHSSEY++ALA+G+IC VG+S Sbjct: 180 DIMPSALAYLGLNPDSHDQADLEKAADLLMKVRPNIRKFHSSEYINALANGDICLAVGFS 239 Query: 245 GDIMQARSRAAEANSGVEIGYTIPKEGAQMFFDNLAIPSDAKNVAEAYELINYLYRPEVA 304 GD+ QAR RAAEA +GV + Y+IP++GAQM+FD LAIP+DA +VAEA+E INY+ +PEV Sbjct: 240 GDVFQARDRAAEAKAGVTVDYSIPEQGAQMWFDMLAIPADAPHVAEAHEFINYMMKPEVI 299 Query: 305 AKNSNFLSYANGNLASQKLIDPKIFTDKNIYPDEAMQKKLFVITARDPATQRIINRLWTR 364 AK SN++ YANGN ASQ+ +D ++ D IYP +A+ +KLF T + QR++ RLWTR Sbjct: 300 AKASNYVFYANGNKASQQFLDKEVLEDTAIYPSDAVMQKLFTTTPFEAKEQRVLTRLWTR 359 Query: 365 VKTGK 369 + TG+ Sbjct: 360 IVTGQ 364 >mlr6533 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Mlo Mesorhizobium loti Length = 365 Score = 470 bits (1209), Expect = e-132, Method: Compositional matrix adjust. Identities = 214/350 (61%), Positives = 276/350 (78%), Gaps = 1/350 (0%) Query: 20 LLSASADAEERTVNFYNWSNYMAPEVLEDFTKETGIKVVYDTFDANETLETRLLAGKSGY 79 L + + AE+R VN +NWS+Y+ V+E FTK+TGIKVVYDTFD+NE LET+LLAG SGY Sbjct: 17 LFTPAGHAEDRVVNVFNWSDYIDSSVIEGFTKQTGIKVVYDTFDSNEILETKLLAGGSGY 76 Query: 80 DVVVPTGYFLQRQITAKVFLKLDKSKLPNLANAWPVVTSQLATYDPGNNYGANYMWGTTG 139 DVVVP+G FL RQI A VF KLDKSKLPN++N W V++ + A YDPGN Y NYMWGT G Sbjct: 77 DVVVPSGNFLARQIQAGVFQKLDKSKLPNISNMWDVISERTAKYDPGNEYSVNYMWGTVG 136 Query: 140 IGYNVKIAEKILGPDAKIDSWDMVFKPENLAKFKDCGIHMLDSADDILPAALSYLGLDPN 199 IGYNVK + LG D KIDSWD+ F PE+LAK KDCG+++LDS DI+PAAL Y+GLDPN Sbjct: 137 IGYNVKKVKAALGTD-KIDSWDVFFNPESLAKLKDCGVYVLDSPADIIPAALKYVGLDPN 195 Query: 200 STKQADLEKAADLVIRIRPYVRKFHSSEYLSALASGEICFVVGWSGDIMQARSRAAEANS 259 ST D+ KA + ++++RP++RKFHSSEY++ALA+G+IC +G+SGD+ QAR+RA EA Sbjct: 196 STSPDDIAKAEEALMKVRPFIRKFHSSEYINALANGDICLAIGFSGDVFQARNRAQEAKQ 255 Query: 260 GVEIGYTIPKEGAQMFFDNLAIPSDAKNVAEAYELINYLYRPEVAAKNSNFLSYANGNLA 319 GVEIGY++PKEGAQ++FD +AIP+DA +VAEA+E INY+ +PEV AK+SNF+ YANGN A Sbjct: 256 GVEIGYSVPKEGAQIWFDQMAIPADAPHVAEAHEFINYMMKPEVIAKSSNFVLYANGNKA 315 Query: 320 SQKLIDPKIFTDKNIYPDEAMQKKLFVITARDPATQRIINRLWTRVKTGK 369 SQ+ +D I D +IYPDEA +KL+ + DP QR+I R WT++ TG+ Sbjct: 316 SQQFVDKAILDDPSIYPDEATVQKLYTVQPYDPKAQRVITRTWTKIVTGQ 365 >BMEI0411 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Bme Brucella melitensis Length = 367 Score = 458 bits (1179), Expect = e-129, Method: Compositional matrix adjust. Identities = 222/357 (62%), Positives = 275/357 (77%), Gaps = 3/357 (0%) Query: 15 VATVLLLSA--SADAEERTVNFYNWSNYMAPEVLEDFTKETGIKVVYDTFDANETLETRL 72 VAT + +A SA A+ER VN YNWS+Y+ +L+DFTKETGIKVVYD +D+NE LET+L Sbjct: 12 VATGFVAAATFSAGAQERVVNIYNWSDYIDDSILKDFTKETGIKVVYDVYDSNEILETKL 71 Query: 73 LAGKSGYDVVVPTGYFLQRQITAKVFLKLDKSKLPNLANAWPVVTSQLATYDPGNNYGAN 132 LAG SGYD+VVP+G FL RQI A VFLKLDK KLPNL N W ++++ ATYDPGN Y N Sbjct: 72 LAGGSGYDLVVPSGEFLGRQIPAGVFLKLDKDKLPNLKNMWDEISTRAATYDPGNEYSVN 131 Query: 133 YMWGTTGIGYNVKIAEKILGPDAKIDSWDMVFKPENLAKFKDCGIHMLDSADDILPAALS 192 YMWGTTGIGYN ++ LG D IDSWD++F PE AK KDCGI++LDSA ++L AL+ Sbjct: 132 YMWGTTGIGYNKAKIKEALGTDT-IDSWDVLFDPEKTAKLKDCGIYLLDSASEMLRPALN 190 Query: 193 YLGLDPNSTKQADLEKAADLVIRIRPYVRKFHSSEYLSALASGEICFVVGWSGDIMQARS 252 YLGLDPNS DL+KA DL ++IRP +RKFHSSEY++ALA+G+IC VG+SGDI QAR Sbjct: 191 YLGLDPNSPSPDDLQKAQDLYLKIRPNIRKFHSSEYINALANGDICMAVGYSGDIFQARD 250 Query: 253 RAAEANSGVEIGYTIPKEGAQMFFDNLAIPSDAKNVAEAYELINYLYRPEVAAKNSNFLS 312 RA +A GVEIGY+IPKEGA ++FD +AIP+DAK+V EA E +NY+ RPEVAAK SN++ Sbjct: 251 RAEKAKQGVEIGYSIPKEGALIWFDQMAIPADAKHVPEALEFMNYMMRPEVAAKASNYVF 310 Query: 313 YANGNLASQKLIDPKIFTDKNIYPDEAMQKKLFVITARDPATQRIINRLWTRVKTGK 369 YANGN ASQK ID +I D IYP + + KKLFV T D TQR++ R WT++ TG+ Sbjct: 311 YANGNKASQKFIDKEILDDPEIYPSDEVMKKLFVPTPYDTKTQRVVTRAWTKIVTGQ 367 >AGc1084 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 365 Score = 444 bits (1142), Expect = e-124, Method: Compositional matrix adjust. Identities = 207/368 (56%), Positives = 273/368 (74%), Gaps = 4/368 (1%) Query: 1 MREQNRRLVSFVGAVATVLLLSASADAEERTVNFYNWSNYMAPEVLEDFTKETGIKVVYD 60 M+ ++ RL A+ T L+ + SA A+E+ V+ YNWS+Y+ P +LEDFTKETGIKVVYD Sbjct: 1 MKNRSLRLTL---ALTTALVAAGSAMAQEKVVHVYNWSDYIDPVILEDFTKETGIKVVYD 57 Query: 61 TFDANETLETRLLAGKSGYDVVVPTGYFLQRQITAKVFLKLDKSKLPNLANAWPVVTSQL 120 +D NE LET+LLAG SGYDVV PT FL RQI A V+ KLDKSKLPNL NAWP +T +L Sbjct: 58 VYDGNEVLETKLLAGSSGYDVVAPTSPFLARQIKAGVYQKLDKSKLPNLKNAWPDITERL 117 Query: 121 ATYDPGNNYGANYMWGTTGIGYNVKIAEKILGPDAKIDSWDMVFKPENLAKFKDCGIHML 180 A YDPGN Y NYMWGTTGIGYNV + LG D +DSWD++FKPEN K K CGI++L Sbjct: 118 AKYDPGNEYAVNYMWGTTGIGYNVAKVKAALG-DVPVDSWDVLFKPENAEKLKSCGINIL 176 Query: 181 DSADDILPAALSYLGLDPNSTKQADLEKAADLVIRIRPYVRKFHSSEYLSALASGEICFV 240 D++D+ A++YLG +P+S + ADLE ++ +IRPYV+ F+SS Y++ LA+G+ C Sbjct: 177 DASDETFAIAMNYLGKNPDSKETADLEAGGEVYSKIRPYVKTFNSSPYINDLANGDTCIS 236 Query: 241 VGWSGDIMQARSRAAEANSGVEIGYTIPKEGAQMFFDNLAIPSDAKNVAEAYELINYLYR 300 +GWSGDI+QA++RA EA +GVE+ Y IPKEG ++ D+ AIP+DAKNV EA+ INY+ + Sbjct: 237 IGWSGDILQAKTRAEEAKNGVEVNYVIPKEGTYIWMDSFAIPADAKNVDEAHAFINYMMK 296 Query: 301 PEVAAKNSNFLSYANGNLASQKLIDPKIFTDKNIYPDEAMQKKLFVITARDPATQRIINR 360 PEVAAK S+++ YANGNL SQ L+DP + + +YPD KKLF I+ P QR++NR Sbjct: 297 PEVAAKASDYVQYANGNLPSQALMDPSVAKNPGVYPDPETMKKLFTISPYGPKEQRVLNR 356 Query: 361 LWTRVKTG 368 +WT++KTG Sbjct: 357 VWTQIKTG 364 >mlr6992 [E] COG0687 Spermidine/putrescine-binding periplasmic protein Mlo Mesorhizobium loti Length = 365 Score = 434 bits (1115), Expect = e-121, Method: Compositional matrix adjust. Identities = 204/363 (56%), Positives = 268/363 (73%), Gaps = 1/363 (0%) Query: 6 RRLVSFVGAVATVLLLSASADAEERTVNFYNWSNYMAPEVLEDFTKETGIKVVYDTFDAN 65 + L+ + VL + + A+++ VN YNWS+Y+ +L+DFTKETGIKVVYDTFD+N Sbjct: 3 KELLGLAVTMTAVLFSAGVSLAQDKVVNVYNWSDYIDSSILDDFTKETGIKVVYDTFDSN 62 Query: 66 ETLETRLLAGKSGYDVVVPTGYFLQRQITAKVFLKLDKSKLPNLANAWPVVTSQLATYDP 125 E LET+LLAG SGYDVVV TG FL RQI A VF KLDKSKLPNL N WP V ++LA YDP Sbjct: 63 EILETKLLAGGSGYDVVVTTGPFLARQIQAGVFQKLDKSKLPNLKNMWPDVMARLAKYDP 122 Query: 126 GNNYGANYMWGTTGIGYNVKIAEKILGPDAKIDSWDMVFKPENLAKFKDCGIHMLDSADD 185 GN Y NYMWGTTGIGYNV + LG D +IDSWD++FKPEN AK KDCGI+++D+ ++ Sbjct: 123 GNEYAVNYMWGTTGIGYNVDKVKAALG-DVQIDSWDVLFKPENAAKLKDCGINIVDAPEE 181 Query: 186 ILPAALSYLGLDPNSTKQADLEKAADLVIRIRPYVRKFHSSEYLSALASGEICFVVGWSG 245 + A++YLG D +S K+ DLE D+ +IR YVR F+SS Y++ LA+G+ C +GWSG Sbjct: 182 TVAVAMNYLGKDGDSKKKEDLEAGGDIYTKIRSYVRTFNSSSYINDLANGDKCISIGWSG 241 Query: 246 DIMQARSRAAEANSGVEIGYTIPKEGAQMFFDNLAIPSDAKNVAEAYELINYLYRPEVAA 305 D++QA++RA EA +GV + Y IP+EG M+FDNLAIP+DAK+V EA+ INYL RPEV A Sbjct: 242 DVLQAKNRAKEAKNGVNVEYLIPREGTYMWFDNLAIPADAKHVDEAHAFINYLMRPEVIA 301 Query: 306 KNSNFLSYANGNLASQKLIDPKIFTDKNIYPDEAMQKKLFVITARDPATQRIINRLWTRV 365 K++N++ ANGNLASQK ID + + ++YP + KKLF I+ QR++ RLWT++ Sbjct: 302 KSTNYVQTANGNLASQKFIDEAVLKNPSVYPPQETLKKLFTISPYGSKEQRVLTRLWTQI 361 Query: 366 KTG 368 KTG Sbjct: 362 KTG 364