BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0200.0106.p (404 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme... 401 e-111 AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Ag... 392 e-108 mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo ... 369 e-102 CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr C... 363 e-100 RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Ri... 344 2e-94 >SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme Sinorhizobium meliloti Length = 424 Score = 401 bits (1031), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/404 (49%), Positives = 270/404 (66%), Gaps = 6/404 (1%) Query: 1 MLYQAYQNHMDLTAPWRTGAAAALRYLNLVPRGASDRT-FGRLAAA-LELISRSSLSYAR 58 M YQ Y+ + + APWRT AA A+R P T FGR AAA LE+ R++ Y + Sbjct: 1 MFYQLYEMNHAMMAPWRT-AADAMRLAFSNPMNPVSHTYFGRAAAAGLEVFERATRRYGK 59 Query: 59 PDYGIGSVTVGNRELEVTEEVAYATPFGSLLHFRKV---DGPEQPRLLLVAPMSGHFATL 115 P++G+ + + + V E + + PF +L+HF + D P++L+VAPMSGH+ATL Sbjct: 60 PEFGLPETEIDGQPVPVRERIVWREPFCNLIHFERSLPRDRAPDPKVLIVAPMSGHYATL 119 Query: 116 LRGTVKTLLQDHDVYITDWHNPRDIPRSKGRFGLEDYTDHLIDFLGQLGPRAHMVAICQP 175 LRGTV+ LL DVYITDW + R +P S+G F L+DY D++I L LGP AH++ +CQP Sbjct: 120 LRGTVEALLPHSDVYITDWIDARMVPLSEGTFDLDDYIDYVIQMLHFLGPDAHVIGVCQP 179 Query: 176 SVSALAAAAIMSEDNHPARPASLTLMAGPIDTRIQPTKVNEFAKSKPIKWFEDNLINYVP 235 +V LAA A+M P P+++TLM GPIDTRI PT VNE AK +PI+WF DN++ VP Sbjct: 180 AVPVLAAVALMEAAEDPLAPSTMTLMGGPIDTRINPTAVNELAKERPIEWFRDNVVMPVP 239 Query: 236 MQCKGALRQVYPGFVQLTAFVSMNLERHIKQHIDLADHLAKGETAKAEVIKTFYDEYFAV 295 G +R VYPGF+QL+ F+SMNL+RH+ H + HL K + AE + FYDEY AV Sbjct: 240 WPQPGFMRMVYPGFLQLSGFMSMNLDRHLIAHKEFFAHLVKNDGDAAEKHRDFYDEYLAV 299 Query: 296 MDLPAEFYIETVRDVFQEHLLPQGMLMHRGRPVNPAAIRRMGLMTVEGEKDDICSIGQTL 355 MDL AEFY++TV+ VF +H LP+G ++HRG+ V+P AIRR+ L+TVEGE DDI +GQT Sbjct: 300 MDLTAEFYLQTVQVVFMQHALPKGEMIHRGKRVDPTAIRRVALLTVEGENDDISGLGQTK 359 Query: 356 AAQDLCTGVRTYRKVHHMQAGVGHYGVFSGKRWSNEIYPLLRDF 399 AAQ +CTG+ + H+MQ VGHYGVF+G R+ EI P + F Sbjct: 360 AAQTICTGIPDSMRQHYMQPDVGHYGVFNGSRFRREIAPRIVAF 403 >AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 418 Score = 392 bits (1006), Expect = e-108, Method: Compositional matrix adjust. Identities = 194/404 (48%), Positives = 262/404 (64%), Gaps = 4/404 (0%) Query: 1 MLYQAYQNHMDLTAPWRTGAAAALRYLNLVPRGASDRTFGR-LAAALELISRSSLSYARP 59 + Y Y+ + AP R GA + N S FGR L A E+ R + Y +P Sbjct: 9 VFYHLYEMNHAAMAPLRAGADMMRQACNNPLNPLSSTAFGRSLDAGFEVFERLTRRYVKP 68 Query: 60 DYGIGSVTVGNRELEVTEEVAYATPFGSLLHFRKVDGPEQ---PRLLLVAPMSGHFATLL 116 ++ +GS +V + + VT+E ++ PF SL+HF + P + P++LLVAPMSGH+ATLL Sbjct: 69 EFDLGSTSVDGQTVVVTDETVWSRPFCSLVHFNRDLEPTRKPDPKVLLVAPMSGHYATLL 128 Query: 117 RGTVKTLLQDHDVYITDWHNPRDIPRSKGRFGLEDYTDHLIDFLGQLGPRAHMVAICQPS 176 RGTV+ LL D+YITDW + R +P ++G F L+DY D++I+ L +GP AH+VA+CQPS Sbjct: 129 RGTVEALLPSADIYITDWADARIVPATEGTFDLDDYIDYVIEMLRHIGPGAHVVAVCQPS 188 Query: 177 VSALAAAAIMSEDNHPARPASLTLMAGPIDTRIQPTKVNEFAKSKPIKWFEDNLINYVPM 236 V LAA ++M D PAS+TLM GPIDTRI PT VN A +KPI+WF DN++ VP Sbjct: 189 VPVLAAVSLMEADGDTFAPASMTLMGGPIDTRINPTAVNGLATAKPIEWFRDNVVMQVPW 248 Query: 237 QCKGALRQVYPGFVQLTAFVSMNLERHIKQHIDLADHLAKGETAKAEVIKTFYDEYFAVM 296 R VYPGF+QL+ F+SMNL++H+ H D +L K + AE + FYDEY AVM Sbjct: 249 PQPAFGRYVYPGFLQLSGFMSMNLDKHMTAHKDFYLNLVKNDGDSAEKHREFYDEYLAVM 308 Query: 297 DLPAEFYIETVRDVFQEHLLPQGMLMHRGRPVNPAAIRRMGLMTVEGEKDDICSIGQTLA 356 DL AEFY++TV VF +H LP+G ++HRGR V+P AI + L TVEGE DDI +GQT A Sbjct: 309 DLTAEFYLQTVETVFIDHALPKGNMLHRGRAVDPTAIHNVALFTVEGENDDISGVGQTKA 368 Query: 357 AQDLCTGVRTYRKVHHMQAGVGHYGVFSGKRWSNEIYPLLRDFV 400 A DLC + ++ H+MQ VGHYGVF+G R+ EI P + DF+ Sbjct: 369 AHDLCRNIPEDKRAHYMQPDVGHYGVFNGSRFRKEIVPRMLDFI 412 >mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo Mesorhizobium loti Length = 425 Score = 369 bits (947), Expect = e-102, Method: Compositional matrix adjust. Identities = 188/404 (46%), Positives = 251/404 (62%), Gaps = 4/404 (0%) Query: 1 MLYQAYQ-NHMDLTAPWRTGAAAALRYLNLVPRGASDRTFGRLAAALELISRSSLSYARP 59 M YQ Y+ NH L A + Y N + A +AA EL R++ Y +P Sbjct: 1 MFYQLYELNHAALQPARLYADAVRMFYSNPLNPIAHTHWGRSVAAGAELFERTTRRYGKP 60 Query: 60 DYGIGSVTVGNRELEVTEEVAYATPFGSLLHFRKV---DGPEQPRLLLVAPMSGHFATLL 116 +G+ V + +EVTE+ ++ PF +L+ F + P+LL+VAPMSGH+ATLL Sbjct: 61 AFGLDKTVVDWKSVEVTEKTVWSKPFCNLVRFERAMPAGRKPDPKLLIVAPMSGHYATLL 120 Query: 117 RGTVKTLLQDHDVYITDWHNPRDIPRSKGRFGLEDYTDHLIDFLGQLGPRAHMVAICQPS 176 RGTV+ +L DV+ITDW + R +P G F L+DY D++I+ LGP H++A+CQPS Sbjct: 121 RGTVEAMLPYADVHITDWVDARMVPLVDGSFDLDDYIDYIIEMFHALGPDTHVMAVCQPS 180 Query: 177 VSALAAAAIMSEDNHPARPASLTLMAGPIDTRIQPTKVNEFAKSKPIKWFEDNLINYVPM 236 V LAA A+M P P+++TLM GPIDTR PT VN A+ K I WF DN++ P Sbjct: 181 VPVLAAVALMEAKGDPFVPSTMTLMGGPIDTRRNPTAVNLLAEEKGIDWFRDNVVMRAPW 240 Query: 237 QCKGALRQVYPGFVQLTAFVSMNLERHIKQHIDLADHLAKGETAKAEVIKTFYDEYFAVM 296 G R+VYPGF+QL+ F+SMNL+RHI H D HL K + AE + FYDEY AVM Sbjct: 241 PVPGFGREVYPGFLQLSGFMSMNLDRHIIAHKDFFMHLVKNDGDNAEKHRDFYDEYMAVM 300 Query: 297 DLPAEFYIETVRDVFQEHLLPQGMLMHRGRPVNPAAIRRMGLMTVEGEKDDICSIGQTLA 356 DL AEFY++TV VF H LP+G + HRG ++ +AIR + L TVEGE DDI +GQT A Sbjct: 301 DLTAEFYLQTVDTVFVRHALPKGEMKHRGAAIDTSAIRNVALFTVEGENDDISGLGQTKA 360 Query: 357 AQDLCTGVRTYRKVHHMQAGVGHYGVFSGKRWSNEIYPLLRDFV 400 AQDLC + +K H+MQ VGHYGVF+G R+ +EI P + DF+ Sbjct: 361 AQDLCINIPADKKAHYMQPAVGHYGVFNGSRFRSEIVPRIVDFI 404 >CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr Caulobacter vibrioides Length = 427 Score = 363 bits (933), Expect = e-100, Method: Compositional matrix adjust. Identities = 185/409 (45%), Positives = 251/409 (61%), Gaps = 9/409 (2%) Query: 1 MLYQAYQNHMDLTAPWRTGAAAALRYLNLVPRGASDRTFGR-LAAALELISRSSLSYARP 59 MLY ++ + P R A AA + A+D GR + A +L + + Y +P Sbjct: 8 MLYALHEAAYYASTPMRLAALAARDFWGSPLNPAADSAVGRRMHAGADLFANVTRRYGKP 67 Query: 60 DYGIGSVTVGNRELEVTEEVAYATPFGSLLHF--------RKVDGPEQPRLLLVAPMSGH 111 + I V VG ++ V V + +P+ L+ F R P +L+VAP+SGH Sbjct: 68 KWNIDKVKVGQVDVRVRPTVVWESPWARLVQFDRDMADMRRAGKFSLDPAVLIVAPLSGH 127 Query: 112 FATLLRGTVKTLLQDHDVYITDWHNPRDIPRSKGRFGLEDYTDHLIDFLGQLGPRAHMVA 171 +ATLLRGTV+ L DH V+I DW N R++ +GRF DY DH+I L LGPR ++VA Sbjct: 128 YATLLRGTVEAFLPDHAVFIVDWVNAREVSVLEGRFDFHDYIDHVIQMLEVLGPRPNVVA 187 Query: 172 ICQPSVSALAAAAIMSEDNHPARPASLTLMAGPIDTRIQPTKVNEFAKSKPIKWFEDNLI 231 +CQP LAAAA+M+E NHP+RPAS+T M PID R+ PT N+ A+ KP WF+ N+I Sbjct: 188 VCQPGPPVLAAAALMAEQNHPSRPASMTFMGSPIDARLSPTVTNQLAEEKPFTWFQSNMI 247 Query: 232 NYVPMQCKGALRQVYPGFVQLTAFVSMNLERHIKQHIDLADHLAKGETAKAEVIKTFYDE 291 VP GA R+VYPGFVQL +F+SMN +RH + H +HL G+ A+ FYDE Sbjct: 248 YNVPPPYAGAGRRVYPGFVQLASFMSMNADRHQEAHRRYFNHLVSGDGDSADKHLEFYDE 307 Query: 292 YFAVMDLPAEFYIETVRDVFQEHLLPQGMLMHRGRPVNPAAIRRMGLMTVEGEKDDICSI 351 Y +V+DL EFY++T+ VFQ++LLP+G L+HRG V P I +GLMTVEGE DDI I Sbjct: 308 YLSVLDLTEEFYLQTIDIVFQQYLLPKGELVHRGTRVKPEVITDIGLMTVEGENDDISGI 367 Query: 352 GQTLAAQDLCTGVRTYRKVHHMQAGVGHYGVFSGKRWSNEIYPLLRDFV 400 GQT AA LC + K+ ++Q VGHYGVF+G+R+ EIYP +R F+ Sbjct: 368 GQTQAAHGLCANIPEDMKLDYVQPSVGHYGVFNGRRFREEIYPRVRKFI 416 >RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Rickettsia prowazekii Length = 423 Score = 344 bits (883), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 164/369 (44%), Positives = 234/369 (63%), Gaps = 2/369 (0%) Query: 35 SDRTFGRLA-AALELISRSSLSYARPDYGIGSVTVGNRELEVTEEVAYATPFGSLLHFRK 93 D F R+ A L L R + Y +P++ I + ++ + E+V + PF L HF+K Sbjct: 42 EDNGFARIIHAYLTLCERMTRKYTKPEFNILETIINDKTYNINEQVIFKKPFCELRHFQK 101 Query: 94 VD-GPEQPRLLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPRSKGRFGLEDY 152 + E P+LL+VAPM+GH ATLLR TV LL D+YITDW +P G F ++DY Sbjct: 102 IGFKKELPKLLIVAPMAGHHATLLRSTVHALLPYTDIYITDWTEANYVPLEAGHFDMDDY 161 Query: 153 TDHLIDFLGQLGPRAHMVAICQPSVSALAAAAIMSEDNHPARPASLTLMAGPIDTRIQPT 212 D+LI+F+ +G H +A+CQP+V LAA ++MSE+N P P+S+ L+ GPID R PT Sbjct: 162 IDYLIEFINFMGQNIHTMAVCQPTVPLLAAISLMSENNSPNVPSSMILIGGPIDARKNPT 221 Query: 213 KVNEFAKSKPIKWFEDNLINYVPMQCKGALRQVYPGFVQLTAFVSMNLERHIKQHIDLAD 272 VNEFA SK ++WF + L VP G R+VYPGF+QLT F+S+NL RHI H++L Sbjct: 222 VVNEFALSKSLEWFCEMLTMQVPPNYPGYGRKVYPGFLQLTGFISLNLLRHIDSHLELWQ 281 Query: 273 HLAKGETAKAEVIKTFYDEYFAVMDLPAEFYIETVRDVFQEHLLPQGMLMHRGRPVNPAA 332 L + KA+ I FYDEY + MD+PAEFY++T+ +VFQ+ L +G + RP++ Sbjct: 282 SLLNSDYQKADYIIKFYDEYLSGMDMPAEFYLQTIDEVFQQFSLARGKFISEKRPIDLKH 341 Query: 333 IRRMGLMTVEGEKDDICSIGQTLAAQDLCTGVRTYRKVHHMQAGVGHYGVFSGKRWSNEI 392 I + L+ +EGE DDI ++GQT AA LC + K +H+Q GVGHYGVFSG ++ I Sbjct: 342 ITKCALLGIEGELDDIAAVGQTKAALKLCVNISESMKRYHLQNGVGHYGVFSGSKFKQFI 401 Query: 393 YPLLRDFVH 401 PL+++F++ Sbjct: 402 VPLIKEFIY 410