BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0194.0027.p (291 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr6339 [U] COG4669 Type III secretory pathway, lipoprotein EscJ... 331 1e-90 PA1723 [U] COG4669 Type III secretory pathway, lipoprotein EscJ ... 188 1e-47 RSp0867 [U] COG4669 Type III secretory pathway, lipoprotein EscJ... 179 5e-45 YPCD1.59 [U] COG4669 Type III secretory pathway, lipoprotein Esc... 178 1e-44 YPO0263 [U] COG4669 Type III secretory pathway, lipoprotein EscJ... 131 2e-30 >mlr6339 [U] COG4669 Type III secretory pathway, lipoprotein EscJ Mlo Mesorhizobium loti Length = 279 Score = 331 bits (849), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 175/274 (63%), Positives = 204/274 (74%), Gaps = 4/274 (1%) Query: 12 GNGSRQSWRRLRVCLALPLLVPLLGCKADLYSKIQEREANEMLALLLGKGVDAARVVAKD 71 G R+S R + LPLLV L GCK DLY+++QEREANEMLALL+ GV A RV AKD Sbjct: 3 GTCGRRSLR----LIVLPLLVALGGCKVDLYTQLQEREANEMLALLMDNGVHAVRVAAKD 58 Query: 72 GTSTIQVEERQLAYSIDLLNVEGLPRQSFKNLGEVFKGSGLVASPIEERARYVYALSEEL 131 GTST+QV+E+ LAYSIDLLN +GLPRQSFKNLGE+F+GSGL+ASP EERARYVYALSEEL Sbjct: 59 GTSTVQVDEKLLAYSIDLLNGKGLPRQSFKNLGEIFQGSGLIASPTEERARYVYALSEEL 118 Query: 132 SRTISDIDGVLSARVHVVLPKNDLLRQDATPSSASVFIRHGSNAKLSALLPQIKMLVANS 191 SRTISDIDGV S RVHVVLP NDLLR ATPSSASVFIRH + A LS LLP+IKMLVA+S Sbjct: 119 SRTISDIDGVFSVRVHVVLPHNDLLRAGATPSSASVFIRHDAKADLSVLLPKIKMLVADS 178 Query: 192 IEGLSYDKVAVVFVPVERTPLEQPASPTAASAQSAKSASTPWLALGVGGAGAIFVIASYV 251 IEGLSYDKV VV V VER+ EQ + P AQ+++ P LA G A A+F +A Y+ Sbjct: 179 IEGLSYDKVEVVLVSVERSAPEQRSVPATVLAQASRPVPAPLLASLTGIAAAVFAVACYL 238 Query: 252 LLGARLRQFGQSSRNLIMFNRRSNVPAVQAAGKK 285 L+ R+ QS+ + RRS V A+ A KK Sbjct: 239 LVTGLSRRRKQSAGEVPKLERRSGVSALDAIRKK 272 >PA1723 [U] COG4669 Type III secretory pathway, lipoprotein EscJ Pae Pseudomonas aeruginosa Length = 248 Score = 188 bits (478), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 95/181 (52%), Positives = 132/181 (72%), Gaps = 4/181 (2%) Query: 36 GCKADLYSKIQEREANEMLALLLGKGVDAARVVAKDGTSTIQVEERQLAYSIDLLNVEGL 95 GCK +LY+ I ++E NEMLALL +GV A + KDGT + VEE +A ++++L +G Sbjct: 22 GCKVELYTGISQKEGNEMLALLRSEGVSADKQADKDGTVRLLVEESDIAEAVEVLKRKGY 81 Query: 96 PRQSFKNLGEVFKGSGLVASPIEERARYVYALSEELSRTISDIDGVLSARVHVVLPKN-D 154 PR++F L +VF GL++SPIEERAR YA ++E+S T+S+IDGVL ARVHVVLP+ D Sbjct: 82 PRENFSTLKDVFPKDGLISSPIEERARLNYAKAQEISHTLSEIDGVLVARVHVVLPEERD 141 Query: 155 LLRQDATPSSASVFIRHGSNAKLSALLPQIKMLVANSIEGLSYDKVAVVFVP---VERTP 211 L + ++P+SASVFI+H ++ +L A +PQIK LV N IEGLSYD+++VV VP V + P Sbjct: 142 GLGRKSSPASASVFIKHAADVQLDAYVPQIKQLVNNGIEGLSYDRISVVLVPSAGVRQVP 201 Query: 212 L 212 L Sbjct: 202 L 202 >RSp0867 [U] COG4669 Type III secretory pathway, lipoprotein EscJ Rso Ralstonia solanacearum Length = 269 Score = 179 bits (455), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 94/219 (42%), Positives = 142/219 (64%), Gaps = 6/219 (2%) Query: 20 RRLRVCLALPLLVPLLGCKADLYSKIQEREANEMLALLLGKGVDAARVVAKDG-TSTIQV 78 RR + +AL L L GC L++++ E +AN+ML +LL G+DA + DG T ++ V Sbjct: 10 RRGALVVALALTTLLAGCNKQLFAQLTEADANDMLTVLLQAGIDAQKTSPDDGKTWSVLV 69 Query: 79 EERQLAYSIDLLNVEGLPRQSFKNLGEVFKGSGLVASPIEERARYVYALSEELSRTISDI 138 ++ A S+++L+ GLPR+ + NLG++FK GL+++P EER R++Y +S++LS+T+S I Sbjct: 70 DDDAFARSMEVLHAHGLPREKYANLGDIFKKDGLISTPTEERVRFIYGVSQQLSQTLSRI 129 Query: 139 DGVLSARVHVVLPKNDLLRQDATPSSASVFIRHGSNAKLSALLPQIKMLVANSIEGLSYD 198 DGV A V +VLP ND L PSSASVFI++ A ++ALLP IK LV +S+EGL+Y+ Sbjct: 130 DGVAVASVQIVLPNNDPLASVVKPSSASVFIKYRPTANVTALLPSIKNLVVHSVEGLTYE 189 Query: 199 KVAVVFVPVERTPLEQPASPTAASAQSAKSASTPWLALG 237 VAV VP + PA A + ++ + S W+ LG Sbjct: 190 NVAVTLVP---GTADDPAIVAATARRAPRGVS--WVMLG 223 >YPCD1.59 [U] COG4669 Type III secretory pathway, lipoprotein EscJ Ype Yersinia pestis Length = 244 Score = 178 bits (451), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 90/181 (49%), Positives = 126/181 (69%), Gaps = 1/181 (0%) Query: 27 ALPLLVPLLGCKADLYSKIQEREANEMLALLLGKGVDAARVVAKDGTSTIQVEERQLAYS 86 L L++ L GCK DLY+ I ++E NEMLALL +G+ A + KDG + VEE +A + Sbjct: 9 TLILILFLTGCKVDLYTGISQKEGNEMLALLRQEGLSADKEPDKDGKIKLLVEESDVAQA 68 Query: 87 IDLLNVEGLPRQSFKNLGEVFKGSGLVASPIEERARYVYALSEELSRTISDIDGVLSARV 146 ID+L +G P +SF L +VF GL++SPIEE AR YA ++E+SRT+S+IDGVL ARV Sbjct: 69 IDILKRKGYPHESFSTLQDVFPKDGLISSPIEELARLNYAKAQEISRTLSEIDGVLVARV 128 Query: 147 HVVLPKNDLLR-QDATPSSASVFIRHGSNAKLSALLPQIKMLVANSIEGLSYDKVAVVFV 205 HVVLP+ + + +SASVFI+H ++ + +PQIK LV NSIEGL+YD+++V+ V Sbjct: 129 HVVLPEEQNNKGKKGVAASASVFIKHAADIQFDTYIPQIKQLVNNSIEGLAYDRISVILV 188 Query: 206 P 206 P Sbjct: 189 P 189 >YPO0263 [U] COG4669 Type III secretory pathway, lipoprotein EscJ Ype Yersinia pestis Length = 243 Score = 131 bits (329), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 79/226 (34%), Positives = 122/226 (53%), Gaps = 17/226 (7%) Query: 20 RRLRVCLALPLL-VPLLGCKADLYSKIQEREANEMLALLLGKGVDAARVVAKDGTSTIQV 78 + LR LA+ L+ + L GC +LYS + E EAN+MLALL+ ++A + + K G + V Sbjct: 2 KSLRQMLAIVLMTLSLSGCDMELYSGLSEGEANQMLALLMLHQINAEKQIEKSGMVGLTV 61 Query: 79 EERQLAYSIDLLNVEGLPRQSFKNLGEVFKGSGLVASPIEERARYVYALSEELSRTISDI 138 ++RQ +++LL G PRQ F + E+F + LV SP +E+A+ V+ ++L +S + Sbjct: 62 DKRQFINAVELLRQNGFPRQRFITVDELFPANQLVTSPTQEQAKMVFLKEQQLENMLSHM 121 Query: 139 DGVLSARVHVVLP-----KNDLLRQDATPSSASVFIRHGSNAKLSALLPQIKMLVANSIE 193 DGV+ A V V +P KN L P +ASVFI++ L + QIK LV +++ Sbjct: 122 DGVIHADVTVAMPMSVDGKNPL------PHTASVFIKYSPEVNLQSYQSQIKGLVRDAVP 175 Query: 194 GLSYDKVAVVFVPVERTPLEQPASPTAASAQSAKSASTPWLALGVG 239 G+ Y K++VV P S + A Q + WL VG Sbjct: 176 GIDYAKISVVMQPANYR-----FSASEAEQQQGPQTTVQWLLRHVG 216