BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0193.0049.p (466 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 506 e-143 PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetr... 480 e-135 Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 452 e-126 mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 239 1e-62 BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tet... 235 2e-61 >PA2326 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 469 Score = 506 bits (1303), Expect = e-143, Method: Compositional matrix adjust. Identities = 250/462 (54%), Positives = 317/462 (68%), Gaps = 2/462 (0%) Query: 4 PKELRFNAFNMMAPSHNWAGLWSHPRDTSLNYNSLDYWVDYARTAERGLLDGIFLADVFG 63 P+E+R NAF M H GLW+HPRD S Y L+YW D AR ERG DG+F+ADV G Sbjct: 2 PREIRLNAFEMNCVGHQSPGLWAHPRDRSSQYKDLEYWTDLARLLERGRFDGLFIADVLG 61 Query: 64 IYDVYGGSPETALRHAVQLPNAEPTLLVSAMAYATKHLGFGITSNLSYEHPYQFARRFST 123 IYDV G+ + A+R A Q+P +P L++ MA T+HLGFG+T++LS+EHPY FARR ST Sbjct: 62 IYDVLDGNGDAAIRQAAQVPVNDPLALITPMAAVTRHLGFGLTASLSFEHPYPFARRLST 121 Query: 124 LDHLTRGRIGWSIVTGYLDSGARGMGLEANRAHDERYEAAEEFLTASYKLWEGSWEDGAV 183 LDHLT+GRIGW++VT YL+SGAR +G +A HD RY+ A+E+L YKL+EGSWEDGAV Sbjct: 122 LDHLTKGRIGWNVVTSYLESGARNLGQQAQLDHDRRYDYADEYLQVLYKLFEGSWEDGAV 181 Query: 184 RRDRTARVFTDPARIHNVRHQGLHYQVDGIHLAEPSPQRTPLLYQAGTSKRGRALAARHA 243 RDR R+F+DP +IH +RH G H+QV GIHL EPSPQRTP+LYQAG S RG+ AA HA Sbjct: 182 LRDRRRRIFSDPGKIHEIRHVGEHFQVPGIHLCEPSPQRTPVLYQAGASSRGKRFAAEHA 241 Query: 244 EAIFLNGQTKPILARAVREIREAAKALGRDPYDIRLFAGATVIVAPTRAEANDLLEEYAG 303 E +F+ +K +L +AV +IR+ GRDP + +F TV++ T A+A +EY Sbjct: 242 ECVFVAAPSKTVLKQAVADIRQRVAEAGRDPRKVLVFNLQTVVLGETDAKARAKFDEYRA 301 Query: 304 YVDQAGQLALLSGWTGIDFSNYRPDQAVQYVESNAIQSMVENF-TLRSDRPVKVGDLATL 362 +V G LAL+SGWTGIDF YRPDQ ++++ +NAIQS VE F + R V +LA Sbjct: 302 FVSYEGALALISGWTGIDFGQYRPDQVLRHIHTNAIQSAVEAFSSADPTRTWTVRELADW 361 Query: 363 SRVGARSPFVVGSPQDVADELIAWVDETDVDGFNLFRLVAPESLTSFVDLVVPELQSRGV 422 +G P VGSP VAD L WV++TDVDGFNL +A E+ + V+L+VPELQ RGV Sbjct: 362 VGIGGFGPLFVGSPATVADLLQEWVEDTDVDGFNLAYALAHETFSDVVELLVPELQKRGV 421 Query: 423 YKTAYREGTLREKLFPGRGPYLDPTHPGAGFRRASAAARTEA 464 YKT Y GTLREKLF G GP L +HP AG+R A EA Sbjct: 422 YKTEYAPGTLREKLF-GDGPRLPDSHPAAGYRDLRALWEKEA 462 >PA2348 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Pae Pseudomonas aeruginosa Length = 462 Score = 480 bits (1236), Expect = e-135, Method: Compositional matrix adjust. Identities = 246/461 (53%), Positives = 310/461 (67%), Gaps = 3/461 (0%) Query: 5 KELRFNAFNMMAPSHNWAGLWSHPRDTSLNYNSLDYWVDYARTAERGLLDGIFLADVFGI 64 K++ NAF+M H GLW+HPRD S +Y L +W + AR ERGL DG+F+AD+ G+ Sbjct: 4 KQILLNAFSMNCVGHINHGLWTHPRDRSSDYRKLRHWTELARLLERGLFDGLFIADILGV 63 Query: 65 YDVYGGSPETALRHAVQLPNAEPTLLVSAMAYATKHLGFGITSNLSYEHPYQFARRFSTL 124 YDVY G E R AVQLP +P +LVSAMA T+HLGFG+T+NL+YE PY FARR STL Sbjct: 64 YDVYQGGIEVTAREAVQLPVNDPLMLVSAMAGVTEHLGFGVTANLTYEAPYPFARRLSTL 123 Query: 125 DHLTRGRIGWSIVTGYLDSGARGMGLEANRAHDERYEAAEEFLTASYKLWEGSWEDGAVR 184 DHL+ GRIGW+IVTGYL+S A+ MG A HD RY+ A+E+L YKLWEGSWED AV Sbjct: 124 DHLSDGRIGWNIVTGYLESAAKAMGQSAQIEHDRRYDRADEYLEVLYKLWEGSWEDDAVL 183 Query: 185 RDRTARVFTDPARIHNVRHQGLHYQVDGIHLAEPSPQRTPLLYQAGTSKRGRALAARHAE 244 DR RV+ P +IH +RH G YQV+G HLAEPSPQRTP+L+QAG+S+RG A AARHAE Sbjct: 184 ADRQRRVYARPEKIHKIRHHGEFYQVEGYHLAEPSPQRTPVLFQAGSSERGLAFAARHAE 243 Query: 245 AIFLNGQTKPILARAVREIREAAKALGRDPYDIRLFAGATVIVAPTRAEANDLLEEYAGY 304 +F++GQT+ + V+ IR A GR DIR+F G VIVA T AEA D EY Y Sbjct: 244 CVFVSGQTREATRQLVQRIRAAVVGAGRRAEDIRIFMGINVIVAATAAEARDKHAEYRRY 303 Query: 305 VDQAGQLALLSGWTGIDFSNYRPDQAVQYVESNAIQSMVENFTL-RSDRPVKVGDLATLS 363 LA + +GID S Y D+ ++Y ++NAI+S+V+N T+ R+D V+ L Sbjct: 304 ASPEAGLAHFASSSGIDLSRYGLDEPIRYEKNNAIESVVKNLTVARTDSTVR--KLLDQL 361 Query: 364 RVGARSPFVVGSPQDVADELIAWVDETDVDGFNLFRLVAPESLTSFVDLVVPELQSRGVY 423 +G R +VG P+ VAD+L W+ +T +DGFNL R V PES F+DLVVPELQ+RG Y Sbjct: 362 ALGGRYVTLVGDPRQVADDLEGWIADTGLDGFNLTRTVEPESYADFIDLVVPELQARGSY 421 Query: 424 KTAYREGTLREKLFPGRGPYLDPTHPGAGFRRASAAARTEA 464 KTAY GTLREKLF L H GA FR+ AA + +A Sbjct: 422 KTAYAPGTLREKLFGDGRARLQAEHAGAAFRQPPAARQRQA 462 >Cgl0986 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 487 Score = 452 bits (1162), Expect = e-126, Method: Compositional matrix adjust. Identities = 226/451 (50%), Positives = 294/451 (65%), Gaps = 2/451 (0%) Query: 5 KELRFNAFNMMAPSHNWAGLWSHPRDTSLNYNSLDYWVDYARTAERGLLDGIFLADVFGI 64 +++ FNAF+M +H GLW+HP+D + +YN+LDYWV A+T E+GL DG+F+ADV G Sbjct: 12 RKILFNAFDMNCVAHQSPGLWTHPKDKARDYNTLDYWVHLAKTLEKGLFDGLFIADVLGT 71 Query: 65 YDVYGGSPETALRHAVQLPNAEPTLLVSAMAYATKHLGFGITSNLSYEHPYQFARRFSTL 124 YDVYG S E AL Q+P +P LLVSAMAYATK+LGFGIT+ +YEHPY FARR +TL Sbjct: 72 YDVYGSSNEAALSSGAQVPVNDPILLVSAMAYATKNLGFGITAGTAYEHPYPFARRLATL 131 Query: 125 DHLTRGRIGWSIVTGYLDSGARGMGLEANRAHDERYEAAEEFLTASYKLWEGSWEDGAVR 184 DHLT GR+GW++VTGYL S A+ MG HDERY+ A+E+L YKL EGSWED AV+ Sbjct: 132 DHLTNGRVGWNVVTGYLPSAAQNMGDTDQLPHDERYDKADEYLEVIYKLLEGSWEDDAVQ 191 Query: 185 RDRTARVFTDPARIHNVRHQGLHYQVDGIHLAEPSPQRTPLLYQAGTSKRGRALAARHAE 244 + VFTD +++H + H G ++ V GI + EPS QRTP++YQAG S RG A +AE Sbjct: 192 NNTETSVFTDSSKVHAINHHGKYFDVPGIAITEPSVQRTPVIYQAGASPRGLKFAGENAE 251 Query: 245 AIFLNGQTKPILARAVREIREAAKALGRDPYDIRLFAGATVIVAPTRAEANDLLEEYAGY 304 A+F+N T + + V +IR AA A GRDP+ +++FA T+I T A+A LEEY+ Y Sbjct: 252 AVFINSSTVEAITKTVAKIRAAAVAAGRDPHAVKIFAMQTIITGETEADAQAKLEEYSRY 311 Query: 305 VDQAGQLALLSGWTGIDFSNYRPDQAVQYVESNAIQSMVENFTLRSDRPV-KVGDLATLS 363 +D G L L+SGWTG D S Y D+ + +ESNAIQS + + V L + Sbjct: 312 IDPVGGLTLMSGWTGADLSQYDLDEPITNIESNAIQSTAATISNGTGEGAWTVRKLGEAT 371 Query: 364 RVGARSPFVVGSPQDVADELIAWVDETDVDGFNLFRLVAPESLTSFVDLVVPELQSRGVY 423 +G P +VGS +VA EL D +DVDGFNL + P + VD VVPELQ Y Sbjct: 372 GIGGFGPVLVGSGANVAAELARIQDLSDVDGFNLAYAITPGTFEDVVDFVVPELQKLSRY 431 Query: 424 KTAYREGTLREKLFPGRGPYLDPTHPGAGFR 454 KT Y G+LR KL G+G LD TH GA +R Sbjct: 432 KTEYAPGSLRNKLL-GKGDRLDDTHRGASYR 461 >mll2278 [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Mlo Mesorhizobium loti Length = 450 Score = 239 bits (610), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 152/418 (36%), Positives = 222/418 (53%), Gaps = 14/418 (3%) Query: 25 WSHPRDTSLNYNSLDYWVDYARTAERGLLDGIFLADVFGIYDVYGGSPETALRHAVQLPN 84 W HPR DY+ + + AER +D IF+AD I+D S ++ + H Sbjct: 21 WRHPRAPGREVLDFDYYRELVQAAERARIDTIFVADHVSIWD----SVKSGVAHYAN-AR 75 Query: 85 AEPTLLVSAMAYATKHLGFGITSNLSYEHPYQFARRFSTLDHLTRGRIGWSIVTGYLDSG 144 EP L+SA+A TKH+G T++ SY PY AR F++LDH+++GR W++VT +D Sbjct: 76 LEPLTLLSALAAVTKHIGLITTASSSYSEPYNVARLFASLDHISKGRASWNVVTSAMDEE 135 Query: 145 ARGMGLEANRAHDERYEAAEEFLTASYKLWEGSWEDGAVRRDRTARVFTDPARIHNVRHQ 204 AR G + N H RYE A EFL LW+ SWED A+ D+ F DP ++H + H+ Sbjct: 136 ARNFGRDGNIEHAFRYERAGEFLDIVKALWD-SWEDEALLIDKETGYFADPDKVHPIDHK 194 Query: 205 GLHYQVDG-IHLAEPSPQRTPLLYQAGTSKRGRALAARHAEAIFLNGQTKPILARAVREI 263 G H++V G ++++ P PQ PL+ QAG+S+ G+ A HA+A F T+ + ++I Sbjct: 195 GKHFKVRGPLNVSRP-PQGHPLIVQAGSSEDGKNFATAHADAHFAIYSTREDGIKYRQDI 253 Query: 264 REAAKALGRDPYDIRLFAGATVIVAPTRAEANDLLEEYAGYV-DQAGQLALLSGWTGIDF 322 E GR P ++ G IVA + AE D E + DQ G + LLS W+GID Sbjct: 254 NERLARHGRRPESFKILPGILPIVAASEAEGRDKQEYLQTLLPDQVG-IDLLSSWSGIDL 312 Query: 323 SNYRPDQAVQYVES----NAIQSMVENFTLRSDRPVKVGDLATLSRVGARSPFVVGSPQD 378 S Y PD + + N ++ + S + + + D+A P V G+P+ Sbjct: 313 SAYPPDGPLPPLPDESTFNGGRTSLNRVKQWSKQNLSLRDIARKLANSGSVPTVAGTPKQ 372 Query: 379 VADELIAWVDETDVDGFNLFRLVAPESLTSFVDLVVPELQSRGVYKTAYREGTLREKL 436 +AD+L W DGFNL + PE +F + VVPELQ RG+ T Y GTLR++L Sbjct: 373 IADQLEDWFVSGAADGFNLMFPLLPEDWVNFAEQVVPELQRRGLVPTEYAPGTLRDRL 430 >BS_yxeK [C] COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases Bsu Bacillus subtilis Length = 441 Score = 235 bits (599), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 148/440 (33%), Positives = 227/440 (51%), Gaps = 21/440 (4%) Query: 2 TRPKELRFNAFNMMAPSHNWAGLWSHPRDTSLNYNSLDYWVDYARTAERGLLDGIFLADV 61 ++ K+++ F +A + + W HP S +LDY+ + A+TAERG LD +FLAD Sbjct: 3 SKKKQIKLGVF--LAGTGHHVASWRHPDAPSDASMNLDYFKELAKTAERGKLDMLFLADS 60 Query: 62 FGIYDVYGGSPETALRHAVQLPNAEPTLLVSAMAYATKHLGFGITSNLSYEHPYQFARRF 121 I + H L EP L+SA+A T +G T++ +Y P+ AR+F Sbjct: 61 LSI---------DSKSHPNVLTRFEPFTLLSALAQVTSKIGLTATASTTYSEPFHIARQF 111 Query: 122 STLDHLTRGRIGWSIVTGYLDSGARGMGLEANRAHDERYEAAEEFLTASYKLWEGSWEDG 181 ++LDHL+ GR GW++VT ++S A E + H RY+ AEEF+ LW+ SWE+ Sbjct: 112 ASLDHLSNGRAGWNVVTSSIESTALNFSGEKHLEHHLRYQRAEEFVEIVKGLWD-SWEED 170 Query: 182 AVRRDRTARVFTDPARIHNVRHQGLHYQVDGIHLAEPSPQRTPLLYQAGTSKRGRALAAR 241 A R++ F D ++H + H+G ++ V G +PQ P++ QAG+S G+ALAA+ Sbjct: 171 AFIRNKETGEFFDKEKMHELNHKGEYFSVRGPLNVSRTPQGQPVIIQAGSSGDGKALAAK 230 Query: 242 HAEAIFLNGQTKPILARAVREIREAAKALGRDPYDIRLFAGATVIVAPTRAEANDLLEEY 301 AE IF + I+E A GRDP I + G I+A T A +E Sbjct: 231 TAEVIFTAQNHLESAQEFYQSIKEQAAEFGRDPEKIAIMPGIFPIIADTEEAAQAKYKEL 290 Query: 302 AGYVDQAGQLALLSGWT-GIDFSNYRPDQAVQYVESNAIQSMVENFTLRSDRPVKVGDLA 360 + + L +L + GID S Y D + +++ A ++ F L + + D Sbjct: 291 QDLIIPSVGLQILQNYLGGIDLSAYPLDGPLPKLDAEASNAVKSRFKLVQEMAER--DNM 348 Query: 361 TLSRV-----GARSPFV-VGSPQDVADELIAWVDETDVDGFNLFRLVAPESLTSFVDLVV 414 T+ + G+R + VG+P+ +AD++ WVD DGFN+ + PE + FVD VV Sbjct: 349 TIRELYKYVAGSRGHHIFVGTPEQLADKMQEWVDTKACDGFNIMPPLLPEGIEVFVDQVV 408 Query: 415 PELQSRGVYKTAYREGTLRE 434 P LQ RGV++ Y TLRE Sbjct: 409 PILQERGVFRKEYEGTTLRE 428