BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0192.0039.p (482 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0343 [P] COG0004 Ammonia permease Rso Ralstonia solanacearum 530 e-150 CC1338 [P] COG0004 Ammonia permease Ccr Caulobacter vibrioides 502 e-142 SMc03807 [P] COG0004 Ammonia permease Sme Sinorhizobium meliloti 460 e-129 AGc5001 [P] COG0004 Ammonia permease Atu Agrobacterium tumefacie... 438 e-122 PA5287 [P] COG0004 Ammonia permease Pae Pseudomonas aeruginosa 407 e-113 >RSc0343 [P] COG0004 Ammonia permease Rso Ralstonia solanacearum Length = 436 Score = 530 bits (1365), Expect = e-150, Method: Compositional matrix adjust. Identities = 275/452 (60%), Positives = 325/452 (71%), Gaps = 20/452 (4%) Query: 31 NKGDNAWMLTSTVLVLLMTIPGLALFYGGLVRSKNMLSVLAQVFYTVCLVTVLWALYGYS 90 NKGD AW+L ST V+LMT+PGLALFYGGLVR KNMLSVL Q L+ +LWA+YGYS Sbjct: 3 NKGDTAWLLVSTAFVILMTLPGLALFYGGLVRKKNMLSVLMQCTGIFSLIMILWAIYGYS 62 Query: 91 LAFTGGSDFIGGFSKAFLMGVTPDSKAATFSVDANISELVYMCFQMTFAAITPALIVGAF 150 +AFT G+ F GG + FL G+TPDS AATFS + E Y FQ FAAIT ALI+GAF Sbjct: 63 IAFTEGNAFFGGLDRLFLKGLTPDSVAATFSKGVVVPEYAYFAFQGAFAAITCALIIGAF 122 Query: 151 AERMKFAAIALFIPLWVTLIYFPIAHMVWYWPGPDAIQDAAKALAAAADGAAKTAAQAKL 210 AER KF+A+ +F+ LW T Y PIAHMVW+WPGPD DA A Sbjct: 123 AERAKFSAVLVFVVLWFTFAYLPIAHMVWFWPGPDGFTDAKAA----------------- 165 Query: 211 DEINADAGWIFKKGAIDFAGGTVVHINAGIAGLVGALLIGKRTGYGKELMAPHSLTMTMI 270 + + A GW+F+KGA+DFAGGTVVHINA +AGLVGA L GKR G+G+E + PHSLT+TM+ Sbjct: 166 EVMTAKGGWLFQKGALDFAGGTVVHINAAVAGLVGAFLFGKRIGFGREALKPHSLTLTMV 225 Query: 271 GASLLWVGWFGFNAGSNLEANGGAALAMTNSFVATAAAALSWMFAEWIIKGHPSLLGALS 330 GASLLW GWFGFNAGS LEANG AALA N+ +ATAAA LSW F EWI K PS+LGA S Sbjct: 226 GASLLWFGWFGFNAGSALEANGSAALAFVNTLLATAAAVLSWSFGEWIGKSKPSMLGAAS 285 Query: 331 GAVAGLVAVTPAAGFAGPMGAIVLGLVVGVVCLFFCTVVKNALGYDDSLDVFGVHCVGGI 390 GAVAGLVA+TPAAGF GPMGAI +G++ GV+CL+ +K LG DD+LDVFGVH VGGI Sbjct: 286 GAVAGLVAITPAAGFVGPMGAIAMGILAGVLCLWGVNGLKRMLGMDDTLDVFGVHGVGGI 345 Query: 391 VGALGTGILVNPALGGAGIIDYTAIPPKVAD-YDFAAQMISQVWGVGTTLVWSGIGSAIL 449 +GAL TG+ +P+LGG GI DY A KVAD Y Q+ Q+ GV TT+VWS + + + Sbjct: 346 MGALLTGVFASPSLGGTGIYDYVA--NKVADGYSIGGQLWIQLQGVLTTIVWSAVVAFVA 403 Query: 450 YKIVDVIVGLRANVETEREGLDITEHTERAYN 481 YKIVD I+GLR E EREGLDIT H E AY Sbjct: 404 YKIVDAIIGLRVPEEEEREGLDITSHGETAYE 435 >CC1338 [P] COG0004 Ammonia permease Ccr Caulobacter vibrioides Length = 510 Score = 502 bits (1293), Expect = e-142, Method: Compositional matrix adjust. Identities = 276/465 (59%), Positives = 337/465 (72%), Gaps = 30/465 (6%) Query: 27 EPTVNKGDNAWMLTSTVLVLLMTIPGLALFYGGLVRSKNMLSVLAQVFYTVCLVTVLWAL 86 E ++KGDNAWMLTS +LVLLM +PGLALFYGGLVR+KNMLSV+ QV + V W L Sbjct: 65 EDKMDKGDNAWMLTSALLVLLMILPGLALFYGGLVRAKNMLSVMMQVSTVALIGFVAWML 124 Query: 87 YGYSLAFTGG---SDFIGGFSKAFLMGVTPDSKAATFSVDANISELVYMCFQMTFAAITP 143 +GYS AFT G F+GG + FL GVTP S ATFS I EL ++ FQ TFAAIT Sbjct: 125 WGYSFAFTDGGSWDKFVGGLGRVFLNGVTPASNVATFSTGVVIPELTFIAFQSTFAAITA 184 Query: 144 ALIVGAFAERMKFAAIALFIPLWVTLIYFPIAHMVWYWPGPDAIQDAAKALAAAADGAAK 203 AL+VG+ ERMKFAAI F LW L Y+PIAHMVW+WPGP+AI Sbjct: 185 ALVVGSLVERMKFAAIVAFAILWPLLSYYPIAHMVWWWPGPNAI---------------- 228 Query: 204 TAAQAKLDEINADAGWIFKKGAIDFAGGTVVHINAGIAGLVGALLIGKRTGYGKELMAPH 263 A A D + + W F GA+DFAGGTVVHINAGIA LVGAL++GKR GYGKE M PH Sbjct: 229 --ALAPEDTVKSGLLWGF--GALDFAGGTVVHINAGIAALVGALILGKRQGYGKEPMPPH 284 Query: 264 SLTMTMIGASLLWVGWFGFNAGSNLEANGGAALAMTNSFVATAAAALSWMFAEWIIKGHP 323 SLT+T++GA LLWVGWFGFNAGSNLE+NG A+LAM N+F+ATA A LSW+ EW+ + P Sbjct: 285 SLTLTLVGAGLLWVGWFGFNAGSNLESNGYASLAMVNTFIATAGAGLSWILVEWVTRKKP 344 Query: 324 SLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVVGVVCLFFCTVVKNALGYDDSLDVFG 383 S LG SG VAGLVAVTPAAGFAGPMGA++LGLVV +C+ FC+VVKNAL YDDSLD FG Sbjct: 345 SALGLASGIVAGLVAVTPAAGFAGPMGALILGLVVSPICIIFCSVVKNALKYDDSLDAFG 404 Query: 384 VHCVGGIVGALGTGILVNPALGGAGIIDYTAIPP-------KVADYDFAAQMISQVWGVG 436 +H +GGIVGA+GTG++VNPA GGAG++DYT+ A YD Q+++Q+ VG Sbjct: 405 IHGIGGIVGAIGTGLVVNPAWGGAGVVDYTSCGKDGDLSTCDTAVYDMGVQLMAQLKAVG 464 Query: 437 TTLVWSGIGSAILYKIVDVIVGLRANVETEREGLDITEHTERAYN 481 T++WS I +AI++ I+ +++GLRA E E +GLDI+EH ERAY+ Sbjct: 465 VTILWSAIATAIVFFIIKLVIGLRAAPEAEEDGLDISEHGERAYH 509 >SMc03807 [P] COG0004 Ammonia permease Sme Sinorhizobium meliloti Length = 451 Score = 460 bits (1183), Expect = e-129, Method: Compositional matrix adjust. Identities = 253/455 (55%), Positives = 314/455 (69%), Gaps = 45/455 (9%) Query: 28 PTVNKGDNAWMLTSTVLVLLMTIPGLALFYGGLVRSKNMLSVLAQVFYTVCLVTVLWALY 87 P +K D +M ST+LVL M IPGLALFYGGLVR+KNMLSVL Q + ++W +Y Sbjct: 39 PVPDKADTTFMFLSTLLVLFMLIPGLALFYGGLVRAKNMLSVLMQCTVIGAAMMIVWVVY 98 Query: 88 GYSLAFTGG-SDFIGGFSKAFLMGVTPDSKAATFSVDANISELVYMCFQMTFAAITPALI 146 GYS AF G S + GGF+K FL GVT DS AATFS I E ++M FQMTFAAITPALI Sbjct: 99 GYSFAFGGSTSAYFGGFAKLFLAGVTVDSTAATFSDGVVIPEYLFMLFQMTFAAITPALI 158 Query: 147 VGAFAERMKFAAIALFIPLWVTLIYFPIAHMVWYWPGPDAIQDAAKALAAAADGAAKTAA 206 VGAFAER+KF+A LF LW T +YFPIAHMVW DA Sbjct: 159 VGAFAERIKFSAAILFAILWATFVYFPIAHMVW---------DAN--------------- 194 Query: 207 QAKLDEINADAGWIFKKGAIDFAGGTVVHINAGIAGLVGALLIGKRTGYGKELMAPHSLT 266 G +F GA+DFAGGTVVHINAG+AGLVGA ++GKRTGYG+++MAPHS+T Sbjct: 195 -----------GLLFGMGALDFAGGTVVHINAGVAGLVGAFMVGKRTGYGRDMMAPHSMT 243 Query: 267 MTMIGASLLWVGWFGFNAGSNLEANGGAALAMTNSFVATAAAALSWMFAEWIIKGHPSLL 326 +T++GA++LW GWFGFNAGSNLEA+GGA LA N+F+ATAAA LSW E + +G S+L Sbjct: 244 LTLVGAAMLWFGWFGFNAGSNLEASGGAVLATVNTFLATAAAVLSWSLVETLTRGKASML 303 Query: 327 GALSGAVAGLVAVTPAAGFAGPMGAIVLGLVVGVVCLFFCTVVKNALGYDDSLDVFGVHC 386 GA SG +AGLVAVTPAAG AGPMGAI++GL+V +C FF +V+KN YDD+ DVFGVH Sbjct: 304 GAASGMIAGLVAVTPAAGIAGPMGAIIMGLIVSPLCYFFVSVIKNKFHYDDTADVFGVHG 363 Query: 387 VGGIVGALGTGILVNPALGGAGIIDYTAIPPKVADYDFAAQMISQVWGVGTTLVWSGIGS 446 VGG GA+ TG+ + +LGG G D +Q+++Q+ V T+VW G+ S Sbjct: 364 VGGFFGAIATGVFASSSLGGIGYAD---------GVTMGSQLMTQITAVAITIVWCGVVS 414 Query: 447 AILYKIVDVIVGLRANVETEREGLDITEHTERAYN 481 AILYK+VD +VGLR ++E EREGLD++ H E AY+ Sbjct: 415 AILYKVVDALVGLRVSMEAEREGLDLSSHGEAAYH 449 >AGc5001 [P] COG0004 Ammonia permease Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 449 Score = 438 bits (1127), Expect = e-122, Method: Compositional matrix adjust. Identities = 257/452 (56%), Positives = 313/452 (69%), Gaps = 46/452 (10%) Query: 31 NKGDNAWMLTSTVLVLLMTIPGLALFYGGLVRSKNMLSVLAQVFYTVCLVTVLWALYGYS 90 K D A+M +T+LVL M IPGLALFYGGLVR+KNMLSVL Q ++ ++W +YGYS Sbjct: 42 EKADTAFMYIATILVLFMIIPGLALFYGGLVRTKNMLSVLMQCTVIGAVMMLVWVIYGYS 101 Query: 91 LAFTGGSDFIGGFS-KAFLMGVTPDSKAATFSVDANISELVYMCFQMTFAAITPALIVGA 149 AF GG+ G K FL GV+ DS +ATFS I E ++ FQMTFAAITPALI+GA Sbjct: 102 FAFGGGTGPYFGGFGKLFLSGVSLDSTSATFSQGVVIYEYTFIAFQMTFAAITPALIIGA 161 Query: 150 FAERMKFAAIALFIPLWVTLIYFPIAHMVWYWPGPDAIQDAAKALAAAADGAAKTAAQAK 209 FAER+KF+A+ LF LW T +YFPIAHMVW DA Sbjct: 162 FAERVKFSAVILFTILWATFVYFPIAHMVW---------DAN------------------ 194 Query: 210 LDEINADAGWIFKKGAIDFAGGTVVHINAGIAGLVGALLIGKRTGYGKELMAPHSLTMTM 269 G IF GA+DFAGGTVVHINAGIAGL+GA+++GKRTG+GK++MAPHS+T+T+ Sbjct: 195 --------GLIFGMGALDFAGGTVVHINAGIAGLIGAIMVGKRTGFGKDMMAPHSMTLTL 246 Query: 270 IGASLLWVGWFGFNAGSNLEANGGAALAMTNSFVATAAAALSWMFAEWIIKGHPSLLGAL 329 +GA++LW GWFGFNAGSNLEA+GGA LA N+F+ATAAA +SW E +G S+LGA Sbjct: 247 VGAAMLWFGWFGFNAGSNLEASGGAVLATMNTFLATAAAIVSWSLVESFTRGKASMLGAA 306 Query: 330 SGAVAGLVAVTPAAGFAGPMGAIVLGLVVGVVCLFFCTVVKNALGYDDSLDVFGVHCVGG 389 SG +AGLVAVTPAAG GPMGAIVLGL+V +C FF +VVKN GYDD+ DVFGVH +GG Sbjct: 307 SGMIAGLVAVTPAAGSVGPMGAIVLGLIVSPICYFFVSVVKNKFGYDDTADVFGVHGIGG 366 Query: 390 IVGALGTGILVNPALGGAGIIDYTAIPPKVADYDFAAQMISQVWGVGTTLVWSGIGSAIL 449 I+GALGTGI +P LGG G AD+ ++Q+ +Q V T+VW + SAIL Sbjct: 367 IIGALGTGIFTSPLLGGTG----------KADFSISSQLWTQFVAVAITIVWCAVVSAIL 416 Query: 450 YKIVDVIVGLRANVETEREGLDITEHTERAYN 481 YKIVDVIVGLR VE EREGLD+ H E AY+ Sbjct: 417 YKIVDVIVGLRVPVEAEREGLDLATHGEAAYH 448 >PA5287 [P] COG0004 Ammonia permease Pae Pseudomonas aeruginosa Length = 442 Score = 407 bits (1046), Expect = e-113, Method: Compositional matrix adjust. Identities = 246/468 (52%), Positives = 305/468 (65%), Gaps = 57/468 (12%) Query: 24 AYAEPTVNKGDNAWMLTSTVLVLLMTIPGLALFYGGLVRSKNMLSVLAQVFYTVCLVTVL 83 A EP +N GD AWML ST LVLLMTIPGLALFYGG+VR+KN+LS++ Q F L+T+L Sbjct: 23 AAEEPVLNSGDTAWMLISTALVLLMTIPGLALFYGGMVRAKNVLSIMMQCFAITGLITIL 82 Query: 84 WALYGYSLAF-TGG--------SDFIGGFSKAFLMGVTPDSKAATFSVDANISELVYMCF 134 W +YGYSLAF T G + F+GG KAFL G+T D S A E V++ F Sbjct: 83 WVVYGYSLAFDTAGMEKGVLNFNSFVGGLDKAFLSGLTADGLT---SATALFPESVFITF 139 Query: 135 QMTFAAITPALIVGAFAERMKFAAIALFIPLWVTLIYFPIAHMVWYWPGPDAIQDAAKAL 194 QMTFA ITPALIVGAFAERMKF+A+ +F+ +W T++Y PIAHMVW Sbjct: 140 QMTFAIITPALIVGAFAERMKFSAMLIFMAVWFTVVYAPIAHMVW--------------- 184 Query: 195 AAAADGAAKTAAQAKLDEINADAGWIFKKGAIDFAGGTVVHINAGIAGLVGALLIGKRTG 254 + D ++ G +DFAGGTVVHINAGIAGLV L++GKR G Sbjct: 185 -------------------SGDGALMWDWGVLDFAGGTVVHINAGIAGLVACLVLGKRKG 225 Query: 255 YGKELMAPHSLTMTMIGASLLWVGWFGFNAGSNLEANGGAALAMTNSFVATAAAALSWMF 314 Y MAPH+L T++GA++LW+GWFGFNAGS ANG A +AM + +ATAAAAL+WMF Sbjct: 226 YPTTPMAPHNLGYTLVGAAMLWIGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALAWMF 285 Query: 315 AEWIIKGHPSLLGALSGAVAGLVAVTPAAGFAGPMGAIVLGLVVGVVCLFFCTVVKNALG 374 AEWI G PS LG SG VAGLVA+TPAAG AGPMGA+V+GL GV+C F T +K AL Sbjct: 286 AEWITHGKPSALGIASGVVAGLVAITPAAGTAGPMGALVIGLASGVICFFAATSLKRALK 345 Query: 375 YDDSLDVFGVHCVGGIVGALGTGILVNPALGGAGIIDYTAIPPKVADYDFAAQMISQVWG 434 YDDSLD FGVH VGGIVGAL TGI P+LGG G ++ D AQ Q G Sbjct: 346 YDDSLDAFGVHAVGGIVGALLTGIFAAPSLGGFGSVE-----------DIGAQFFVQFKG 394 Query: 435 VGTTLVWSGIGSAILYKIVDVIVGLRANVETEREGLDITEHTERAYNM 482 V T+V++ + + ++ K++D+++GLR E E GLD+ H ER YN+ Sbjct: 395 VAFTVVYTAVVTFVILKVLDLVMGLRVTEEEEAVGLDLALHNERGYNL 442