BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0192.0002.p (247 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1213 [V] COG0534 Na+-driven multidrug efflux pump Rso Ralston... 260 2e-69 BS_yoeA [V] COG0534 Na+-driven multidrug efflux pump Bsu Bacillu... 195 7e-50 mlr1521 [V] COG0534 Na+-driven multidrug efflux pump Mlo Mesorhi... 143 4e-34 RSp0164 [V] COG0534 Na+-driven multidrug efflux pump Rso Ralston... 130 2e-30 lin0003 [V] COG0534 Na+-driven multidrug efflux pump Lin Listeri... 82 8e-16 >RSp1213 [V] COG0534 Na+-driven multidrug efflux pump Rso Ralstonia solanacearum Length = 455 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 138/251 (54%), Positives = 181/251 (72%), Gaps = 4/251 (1%) Query: 1 VVTLLWLHVHMLRHRHALAFDAAFLRAMRPNGALLRIVLRLGIPTAIGMVVVSLAELVLL 60 +V L WL H+ R +H LA DAA R +R + +L+ VL++G+P+ + +VV+S+AEL LL Sbjct: 205 LVALSWLAFHLRRRQHPLAPDAALARHLRIDAKVLKTVLKIGVPSGVQLVVISIAELSLL 264 Query: 61 GLVNGFGSDATAAYGAVNQVIGYVQFPAISIAISVSIFGAQAIGRGDSARVGAIVRTGIE 120 LVN FGSDATAAYGA Q+I YVQFPA+SIAI+ SI GAQAIG G R+ AI RTG+ Sbjct: 265 SLVNRFGSDATAAYGAGTQIISYVQFPAMSIAITASILGAQAIGAGRPDRLDAITRTGLW 324 Query: 121 MNLALTGGLVVLGYLFARPLMGFFIVDNAVLALAQQLLYIVLWSMVLFGMATVFSGAMRA 180 +N+A++G LVV+ LF+R ++G F AV+ L Q LL+I LWS V+FGMA+VF+G MRA Sbjct: 325 LNVAISGLLVVVALLFSRAVVGLFTRSAAVIDLTQSLLHISLWSSVVFGMASVFAGVMRA 384 Query: 181 GGTVWMPLLLSTLAIAVVEVPVAILLSRASGVSGIWIAYPVTFATMFVLQMAYYALVWRK 240 GTV+ P +S LAIA VEVPVA +L R G +GIW AYP F TMFV+Q YY LVWR+ Sbjct: 385 SGTVFAPTAISILAIAAVEVPVAWMLCRHIGTNGIWAAYPAAFITMFVMQGLYYGLVWRR 444 Query: 241 QT----IKRLI 247 + I+R++ Sbjct: 445 RAQRFGIRRMV 455 >BS_yoeA [V] COG0534 Na+-driven multidrug efflux pump Bsu Bacillus subtilis Length = 463 Score = 195 bits (496), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 108/249 (43%), Positives = 160/249 (64%), Gaps = 2/249 (0%) Query: 1 VVTLLWLHVHMLRHRHALAFDAAFLRAMRPNGALLRIVLRLGIPTAIGMVVVSLAELVLL 60 + T L L V++ + +H L FD R ++ + LL ++LRLG+P +I M++VSL+E+ ++ Sbjct: 214 IATFLVLMVYLRKRKHPLQFDKTVRRYLKMDKELLVLLLRLGVPASINMILVSLSEIAVI 273 Query: 61 GLVNGFGSDATAAYGAVNQVIGYVQFPAISIAISVSIFGAQAIGRGDSARVGAIVRTGIE 120 VN +GS+ATAAYG VNQV YVQ PA+S+ I+VSIF AQ+IG + R+ ++R GI Sbjct: 274 SFVNHYGSNATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFDRLKQVIRVGIW 333 Query: 121 MNLALTGGLVVLGYLFARPLMGFFIVDNAVLALAQQLLYIVLWSMVLFGMATVFSGAMRA 180 +N + G L++L Y+F+ ++ F+ + L +A +LL LWS +LFG A + S MRA Sbjct: 334 LNYIIGGVLIILIYVFSHQILSLFLTEQESLYIAHRLLMTTLWSYLLFGNAQIISATMRA 393 Query: 181 GGTVWMPLLLSTLAIAVVEVPVAILLSRASGVS--GIWIAYPVTFATMFVLQMAYYALVW 238 GTV P ++S AI VEVPVA +LS + + G+W+ YP FA +L YY VW Sbjct: 394 SGTVLWPTVISIFAIWGVEVPVAFVLSHYTKLEILGVWVGYPAAFAVSLLLIYGYYQFVW 453 Query: 239 RKQTIKRLI 247 +K+ I RLI Sbjct: 454 KKKQITRLI 462 >mlr1521 [V] COG0534 Na+-driven multidrug efflux pump Mlo Mesorhizobium loti Length = 486 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 85/234 (36%), Positives = 133/234 (56%), Gaps = 1/234 (0%) Query: 2 VTLLWLHVHMLRHRHALAFDAAFLRAMRPNGALLRIVLRLGIPTAIGMVVVSLAELVLLG 61 V+++ + V + +H L A L +RP+ ALLRIV+ G+P + M+V+S A L ++G Sbjct: 212 VSVIAILVVLYSRKHPLRLAGANLALLRPDPALLRIVVFKGVPMGLQMIVISAAALTVMG 271 Query: 62 LVNGFGSDATAAYGAVNQVIGYVQFPAISIAISVSIFGAQAIGRGDSARVGAIVRTGIEM 121 +VN +GS AAYG Q+ Y+Q PA++I +VS AQ +G G R+G + +G+ Sbjct: 272 IVNSYGSQVAAAYGIAAQLWTYIQMPALAIGAAVSSMAAQNVGAGRWDRIGRVAASGVGF 331 Query: 122 NLALTGGLVVLGYLFARPLMGFFI-VDNAVLALAQQLLYIVLWSMVLFGMATVFSGAMRA 180 NL LTG LV L +LF RP++G F+ D+A + +A + WS +LFG+ V +RA Sbjct: 332 NLVLTGALVALLWLFDRPILGLFLSSDSAAIDIAAHINTAASWSFILFGITIVLFATVRA 391 Query: 181 GGTVWMPLLLSTLAIAVVEVPVAILLSRASGVSGIWIAYPVTFATMFVLQMAYY 234 G V PL++ +++ +V A + G +W ++P T VL AYY Sbjct: 392 TGAVMPPLIILVISVLIVRTGFAYFMRGVIGEEALWWSFPAGSITSLVLAAAYY 445 >RSp0164 [V] COG0534 Na+-driven multidrug efflux pump Rso Ralstonia solanacearum Length = 482 Score = 130 bits (327), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 76/226 (33%), Positives = 129/226 (57%), Gaps = 1/226 (0%) Query: 10 HMLRHRHALAFDAAFLRAMRPNGALLRIVLRLGIPTAIGMVVVSLAELVLLGLVNGFGSD 69 + R RH L A +RP+ +L ++ G+P + M VVS+A ++++ LVN FG+ Sbjct: 224 RLYRTRHLLCLRADEAHLLRPDRGILAALVAKGLPMGLHMAVVSMAAVMMMSLVNRFGAQ 283 Query: 70 ATAAYGAVNQVIGYVQFPAISIAISVSIFGAQAIGRGDSARVGAIVRTGIEMNLALTGGL 129 +AAYGA Q+ Y+Q P +++ ++V+ AQ +G RVG I RTGI +N+A+ GG+ Sbjct: 284 LSAAYGACLQLWNYIQLPVLAMGMAVTPMAAQNLGARRLDRVGRIARTGIALNIAMAGGM 343 Query: 130 VVLGYLFARPLMGFFI-VDNAVLALAQQLLYIVLWSMVLFGMATVFSGAMRAGGTVWMPL 188 V++ F+R ++G F+ L AQ + +V W+ V G+ V +RA GT PL Sbjct: 344 VLVLQCFSRAVLGLFLPAQPETLQAAQHINAVVAWAFVPVGVTFVLGSVIRASGTAIPPL 403 Query: 189 LLSTLAIAVVEVPVAILLSRASGVSGIWIAYPVTFATMFVLQMAYY 234 ++ LA+ + P A+LL A G +W+++ + + ++ +AYY Sbjct: 404 VILFLALWCIRQPAAVLLMDAWGADALWLSFGLGSVSSMLMSLAYY 449 >lin0003 [V] COG0534 Na+-driven multidrug efflux pump Lin Listeria innocua Length = 447 Score = 82.4 bits (202), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 67/236 (28%), Positives = 116/236 (49%), Gaps = 7/236 (2%) Query: 13 RHRHALAFDAAFLRAMRPNGALLRIVLRLGIPTAIGMVVVSLAELVLLGLVNGFGSDATA 72 RH + + A + P LL+ ++R+G+P+ + +S+ + L L+NGFGS A Sbjct: 214 RHVSFMRIERAKFKLSTP---LLKEMVRIGLPSGLQGSFISIGNMALQSLINGFGSSVVA 270 Query: 73 AYGAVNQVIGYVQFPAISIAISVSIFGAQAIGRGDSARVGAIVRTGIEMNLALTGGLVVL 132 AY A +++ P I+ + S+F Q IG G RV +GI++ A++ G+ +L Sbjct: 271 AYTAASRIDSLTYQPGIAFGAASSMFAGQNIGAGKIDRVREGFWSGIKVVTAISIGITIL 330 Query: 133 GYLFARPLMGFFI--VDNAVLALAQQLLYIVLWSMVLFGMATVFSGAMRAGGTVWMPLLL 190 LFAR + F+ + V+ + L IV V+ G+ V +R G +PL + Sbjct: 331 VQLFARQFLLLFVDSSETEVINIGVSYLLIVSLFYVVVGILFVVRETLRGTGDAMVPLAM 390 Query: 191 STLAIAVVEVPVAILLSRASGVSGIWIAYPVTFATMFVLQM-AYYALVWRKQTIKR 245 + V + + +LS G G+W A PV + T +L + Y + W+K+ + R Sbjct: 391 GIFEL-VSRLAIGFILSLYIGYVGLWWATPVAWITATMLGVWRYKSGAWQKKAVIR 445