BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0190.0025.p (364 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the T... 124 2e-28 slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the T... 119 8e-27 lin0862 [R] COG2159 Predicted metal-dependent hydrolase of the T... 108 3e-23 SA2366 [R] COG2159 Predicted metal-dependent hydrolase of the TI... 105 1e-22 Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the T... 65 2e-10 >AGc4595 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 327 Score = 124 bits (312), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 94/299 (31%), Positives = 142/299 (47%), Gaps = 44/299 (14%) Query: 88 DKRLAAMDAQAVDMEVLSIN-------PFWYDRDRDLAAQIVKIQNEKLAELCASKPDRF 140 D RL MDA ++ +LS+N P D+ A +I + N+ LAE CA +PDRF Sbjct: 41 DTRLKLMDAHGIETMILSLNAPAVQAIP-----DKTKAIEIARRANDALAEECAKRPDRF 95 Query: 141 AAFASLTLQAPDLAVQELETAVKKQGLKGAAIGGVVDGAE------FSNPKFHPIWAKAE 194 AFA+L LQ PD A QEL V G GA + G + + P++ P W + E Sbjct: 96 LAFAALPLQDPDAATQELRRCVNDLGFVGALVNGFSQEGDGQTPLYYDLPQYRPFWGEVE 155 Query: 195 ELGVPLFIHPQG-VPELNKRLSGNGW-LGNTIGNPLETTIALSHLIFEGTLDRFPGLRII 252 +L VP ++HP+ +P+ ++ G+ W LG T ET + L+ G D P L II Sbjct: 156 KLDVPFYLHPRNPLPQDSRIYDGHPWLLGPTWAFAQETAVHALRLMASGLFDEHPRLDII 215 Query: 253 AAHGGGYLGSYADRSDHACLVGPKGCNPEIKL-QKAPAEYLKQIYFDSLI-------FTP 304 H G L R DH N +KL + PA+ YF+ F Sbjct: 216 LGHMGEGLPYMMWRIDHR--------NAWVKLPPRYPAKRRFVDYFNENFHITTSGNFRT 267 Query: 305 EAIRHLAAQVGAGQIVLGSDYPYPWQLAPVDH---IFASSSLSDDDKANILGRTAAKLL 360 + + ++GA +I+ +D+P+ +DH F ++S+++ D+ I GRT A+ L Sbjct: 268 QTLIDAILEIGADRILFSTDWPF----ENIDHASDWFHATSIAEADRVKI-GRTNARRL 321 >slr0619 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Syn Synechocystis Length = 348 Score = 119 bits (299), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 94/314 (29%), Positives = 150/314 (47%), Gaps = 24/314 (7%) Query: 58 FREAGALLGADAGKLQLPPVNGASEAFIEIDK-RLAAMDAQAVDMEVLSI-NPFWYDRDR 115 FR+ G + AD+ P E I++ R+A MDA +D++V+SI +P + Sbjct: 34 FRKMGETILADS-----PGTRIIHERLIDLGAGRIAHMDATGIDIQVISITSPGVQVFEP 88 Query: 116 DLAAQIVKIQNEKLAELCASKPDRFAAFASLTLQAPDLAVQELETAVKKQGLKGAAIGGV 175 +LA + + N++L+E + PDRFA A++ Q+P A E+E A++K GL G I Sbjct: 89 ELATTLARQANDQLSEAIKNHPDRFAGLAAVAPQSPQTAALEIERAIQKLGLCGVLINSH 148 Query: 176 VDGAEFSNPKFHPIWAKAEELGVPLFIHPQG------VPELNKRLSGNGWLGNTIGNPLE 229 G + K+ I+A AE P+++HP+ P L+ L GW G +E Sbjct: 149 TGGEYLDDQKYWEIFAAAEANSAPIYLHPRTPSPEMISPFLDYGLYFAGW-----GFTVE 203 Query: 230 TTIALSHLIFEGTLDRFPGLRIIAAHGGGYLGSYADRSDHA-CLVGPKGCNPEIKLQKAP 288 T LI G D FP L I+ H G L + R D+ L G P K+ + P Sbjct: 204 TATHGLRLIMGGVFDHFPKLTIVLGHMGEGLPYWLQRLDNRYSLQVEIGAVP--KMPRLP 261 Query: 289 AEYLKQIYF--DSLIFTPEAIRHLAAQVGAGQIVLGSDYPYPWQLAPVDHIFASSSLSDD 346 +EY K + S + +P A+RH+ +G+ ++ +DYPY + ++ +SD Sbjct: 262 SEYFKDHFLITTSGVCSPPALRHVLEVLGSDAVLFAADYPYE-SVEEAVSFLDNAPISDQ 320 Query: 347 DKANILGRTAAKLL 360 ++ I A KL Sbjct: 321 ERRKIYQTNAEKLF 334 >lin0862 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Lin Listeria innocua Length = 332 Score = 108 bits (269), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 79/292 (27%), Positives = 134/292 (45%), Gaps = 29/292 (9%) Query: 86 EIDKRLAAMDAQAVDMEVLSI-NPFWYDRDRDLAAQIVKIQNEKLAELCASKPDRFAAFA 144 + +KRLA MD +DM+VLS N + ++ + K N++LA++ P RFA FA Sbjct: 51 DTEKRLAFMDQHGIDMQVLSYGNSSPQNLGPKISVALCKRANDELAKVIQQNPTRFAGFA 110 Query: 145 SLTLQAPDLAVQELETAVKKQGLKGAAIGGVVDGAEFSNPKFHPIWAKAEELGVPLFIHP 204 L + +P+ A EL+ AV++ G KGA + G + F N ++PI+ AE+L VP+ HP Sbjct: 111 VLPVGSPEDAAIELKRAVEELGFKGALVKGQFENKYFDNSFYYPIFEMAEKLDVPISFHP 170 Query: 205 QGVPE--LNKRLSGNGWLGNT--------IGNPLETTIALSHLIFEGTLDRFPGLRIIAA 254 +PE + + + W G ++ I + +I G D+ P L+II Sbjct: 171 SFIPEPITEQYFASDAWSDVVTGVFSSAGFGWHMDVGIQVVRMILSGIFDKLPNLKIITG 230 Query: 255 HGGGYLGSYADRSDHACLVGPKGCNPEIKLQKAPAEYLKQIYFDSLIFTPEAI------R 308 H G + + +R D L++ ++Y Y ++ TP + + Sbjct: 231 HLGEMVPMFLNRMDDT-------LGHWTTLERKISDY----YRTNVYVTPSGLLYKNEWK 279 Query: 309 HLAAQVGAGQIVLGSDYPYPWQLAPVDHIFASSSLSDDDKANILGRTAAKLL 360 L ++ ++ DYP+ + + L+D+ KA I A KLL Sbjct: 280 FLLNELDENHLIYALDYPFV-KTENAGTFLDTLDLTDEVKAKIAHENAEKLL 330 >SA2366 [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Sau Staphylococcus aureus N315 Length = 336 Score = 105 bits (263), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 82/295 (27%), Positives = 134/295 (45%), Gaps = 33/295 (11%) Query: 88 DKRLAAMDAQAVDMEVLSI-NPFWYDRDRDLAAQIVKIQNEKLAELCASKPDRFAAFASL 146 D+R+ M+ Q V ++VLS N + A ++ + N++LA A P+RF FA+L Sbjct: 55 DERIQFMNNQDVQIQVLSYGNGSPSNLVGQKAIELCQKANDQLANYIAQYPNRFVGFATL 114 Query: 147 TLQAPDLAVQELETAVKKQGLKGAAIGGVVDGAEFSNPKFHPIWAKAEELGVPLFIHPQG 206 + P+ A +E E + G KGA I G K+ I+ AE L VP+++HP Sbjct: 115 PINEPEAAAREFERCINDLGFKGALIMGRAQDGFLDQDKYDIIFKTAENLDVPIYLHPAP 174 Query: 207 VPELNKRLSGNGWLGNT------------IGNPLETTIALSHLIFEGTLDRFPGLRIIAA 254 V N + + + GN G ++ I HL+ G DR+P L +I Sbjct: 175 V---NSDIYQSYYKGNYPEVTAATFACFGYGWHIDVGIHAIHLVLSGIFDRYPKLNMIIG 231 Query: 255 HGGGYLGSYADRSDHACLVGPKGCNPEIKLQKAPAEYLKQIYF--DSLIFTPEAIRHLAA 312 H G ++ + +R D A L + + Y K ++ S + T + Sbjct: 232 HWGEFIPFFLERMDEALFAE--------HLNHSVSYYFKNSFYITPSGMLTKPQFDLVKK 283 Query: 313 QVGAGQIVLGSDYPY--PWQLAPVDHIFASS-SLSDDDKANILGRTAAKLLGVAA 364 +VG +I+ +DYPY P +L +F L+D++K I AKLLG+++ Sbjct: 284 EVGIDRILYAADYPYIEPEKLG----VFLDELGLTDEEKEKISYTNGAKLLGLSS 334 >Rv2303c [R] COG2159 Predicted metal-dependent hydrolase of the TIM-barrel fold Mtu Mycobacterium tuberculosis H37Rv Length = 307 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 52/172 (30%), Positives = 76/172 (44%), Gaps = 23/172 (13%) Query: 121 IVKIQNEKLAELCASKPDRFAAFASLTLQAPDLAVQELETAVKKQGLKGAAIGGVVDGAE 180 + + N +A + A PDRF FA + A D AV E E V + G +G ++ + G Sbjct: 91 VTFVHNASVAAVAARYPDRFLPFAGADILAGDSAVDEFERWVVEHGFRGLSLRPFMIGRP 150 Query: 181 FSNPKFHPIWAKAEELGVPLFIHPQGVPELNKRLSGNGWLGNTIGNPLETTIALSHLIFE 240 S+P + P +AK ELGVP+ IH RLS +G+P H+ + Sbjct: 151 ASDPAYFPCYAKCVELGVPVSIH-TSADWTRTRLSD-------LGHP-------RHI--D 193 Query: 241 GTLDRFPGLRIIAAHGGGYLGSYADRSDHACLVGPKGCNPEIKLQKAPAEYL 292 RFP L I+ +HGG ACL+ K N ++L +Y Sbjct: 194 DVACRFPELTILMSHGG------YPWVLQACLIAWKHPNVYLELAAHRPKYF 239