BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0182.0006.p (813 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2715 [E] COG2873 O-acetylhomoserine sulfhydrylase Mac Methanos... 343 8e-94 TM0882 [E] COG2873 O-acetylhomoserine sulfhydrylase Tma Thermoto... 343 1e-93 lin0604 [E] COG2873 O-acetylhomoserine sulfhydrylase Lin Listeri... 341 4e-93 mlr8465 Non COG 339 2e-92 PA5025 [E] COG2873 O-acetylhomoserine sulfhydrylase Pae Pseudomo... 337 5e-92 >MA2715 [E] COG2873 O-acetylhomoserine sulfhydrylase Mac Methanosarcina acetivorans str.C2A Length = 441 Score = 343 bits (881), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 188/459 (40%), Positives = 278/459 (60%), Gaps = 19/459 (4%) Query: 356 MGTPSTRQACERGVEMSSSFAKDSQLPFSAETAVLHAGYRRDPVTKAVAVPIYQNTAYEL 415 MG + Q E+G++ + + P T +HAG + DPVT A +VPIYQ AY Sbjct: 1 MGNKNENQTGEKGLKR-----RALENP-GISTLAVHAGAKPDPVTGARSVPIYQTAAYVF 54 Query: 416 DGDLNHIADVYNVKADGFTYTRIINPTTRALEKRYAAVDMGSDSLAVASGQAATFLAIVN 475 D D H AD++ ++ +G YTR++NPTT EKR AA++ G +LA +SG AA A++ Sbjct: 55 D-DTEHAADLFGLRKEGNIYTRLMNPTTDVFEKRIAALEGGIGALATSSGMAAITTALLT 113 Query: 476 LSSGEVGDNIVASPYLYGNTWNLLHNTLKRLGISVRTADPRRPETFERAIDDRTICLFGE 535 + GD IV+ +YG T+ L + T +LG +V+ D PE F++ I +T L+ E Sbjct: 114 FTG--PGDEIVSGDKIYGGTYELFNYTFPKLGRTVKFVDSSSPEEFKKEITGKTKALYVE 171 Query: 536 VISNPCLIPLPVKQLAEIGRKHGVPLVVDNTTTPLVCRPSDLGAAITTYSATKYICGHGT 595 I NP L ++LAEI +PLVVDNT P++ RP + GA I +SATKYI GHGT Sbjct: 172 SIGNPKLDVPDFEKLAEIAHTAEIPLVVDNTVAPVILRPIEHGADIVVHSATKYIGGHGT 231 Query: 596 TLGGLIVDNGKFSYRGASRFPLFNSPDEAHGGIIWRNALQDVDDLGKSEVLLKARMTWLR 655 ++GG+I+D+G F + + +FP PD + G+ ++ A GK+ + KAR+ ++R Sbjct: 232 SIGGVIIDSGNFDWS-SEKFPEICDPDPGYHGLKYKEA------FGKAAFIAKARVQFMR 284 Query: 656 DTGAAIAPFASFQLIQGLETLPLRMKQHCANARIVADVLKEHPKVRRVFYPGLFEGADRE 715 D GA ++PF SF GLETLPLR+K+HC NA VA L+ HPKV V YPGL E Sbjct: 285 DIGACLSPFNSFLFTLGLETLPLRVKKHCDNALAVARFLQAHPKVAWVSYPGLESHRSHE 344 Query: 716 TVDQTLNPAYGHGAMVMFEVE-DEQAGRKFIQNVDLMYHVSNVGDARTLVTHPVSTTHTT 774 + L G+GA++ F ++ +A +KF+++++L H++N+GDA++LV HP STTH Sbjct: 345 LAKKYLKS--GYGAIIGFGIKGGTEATKKFVESLELFSHLANIGDAKSLVIHPASTTHEQ 402 Query: 775 VPREKREAAGIFGGSIRLCVGIEDVDDIVRDLDRALSAI 813 + E++ A G+ IRL +GIED D++ DL +ALS + Sbjct: 403 LSAEEQRACGVTEDFIRLSIGIEDEKDLIFDLGQALSEV 441 >TM0882 [E] COG2873 O-acetylhomoserine sulfhydrylase Tma Thermotoga maritima Length = 430 Score = 343 bits (880), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 193/431 (44%), Positives = 266/431 (61%), Gaps = 15/431 (3%) Query: 383 FSAETAVLHAGYRR-DPVTKAVAVPIYQNTAYELDGDLNHIADVYNVKADGFTYTRIINP 441 + T LHAGY + T + AVPIYQ T+Y D +H A ++ ++ GF YTRI NP Sbjct: 6 YGYNTRALHAGYEPPEQATGSRAVPIYQTTSYVFR-DSDHAARLFALEEPGFIYTRIGNP 64 Query: 442 TTRALEKRYAAVDMGSDSLAVASGQAATFLAIVNLSSGEVGDNIVASPYLYGNTWNLLHN 501 T LE+R AA++ G +LAVASGQAA AI+N++ GD IV+ LYG T+NL + Sbjct: 65 TVSVLEERIAALEEGVGALAVASGQAAITYAILNIAG--PGDEIVSGSALYGGTYNLFRH 122 Query: 502 TL-KRLGISVRTADPRRPETFERAIDDRTICLFGEVISNPCLIPLPVKQLAEIGRKHGVP 560 TL K+ GI V+ D P+ E AI ++T ++ E I NP L + +AEI +HGVP Sbjct: 123 TLYKKSGIIVKFVDETDPKNIEEAITEKTKAVYLETIGNPGLTVPDFEAIAEIAHRHGVP 182 Query: 561 LVVDNTTTPLVCRPSDLGAAITTYSATKYICGHGTTLGGLIVDNGKFSYRGASRFPLFNS 620 L+VDNT P + RP + GA I YSATK+I GHGT++GGLIVD+GKF + +FP Sbjct: 183 LIVDNTVAPYIFRPFEHGADIVVYSATKFIGGHGTSIGGLIVDSGKFDWTNG-KFPELVE 241 Query: 621 PDEAHGGIIWRNALQDVDDLGKSEVLLKARMTWLRDTGAAIAPFASFQLIQGLETLPLRM 680 PD ++ G+ + V+ ++ + K R LRD G+ ++PF +F I GLETL LRM Sbjct: 242 PDPSYHGVSY------VETFKEAAYIAKCRTQLLRDLGSCMSPFNAFLFILGLETLSLRM 295 Query: 681 KQHCANARIVADVLKEHPKVRRVFYPGLFEGADRETVDQTLNPAYGHGAMVMFEVE-DEQ 739 K+HC NA + + LK HP V V YP RE + L G+GA+V F V+ ++ Sbjct: 296 KKHCENALKIVEFLKSHPAVSWVNYPIAEGNKTRENALKYLKE--GYGAIVTFGVKGGKE 353 Query: 740 AGRKFIQNVDLMYHVSNVGDARTLVTHPVSTTHTTVPREKREAAGIFGGSIRLCVGIEDV 799 AG+KFI ++ L+ H++N+GDARTL HP STTH + E++ G+ IRL VGIEDV Sbjct: 354 AGKKFIDSLTLISHLANIGDARTLAIHPASTTHQQLTEEEQLKTGVTPDMIRLSVGIEDV 413 Query: 800 DDIVRDLDRAL 810 +DI+ DLD+AL Sbjct: 414 EDIIADLDQAL 424 >lin0604 [E] COG2873 O-acetylhomoserine sulfhydrylase Lin Listeria innocua Length = 425 Score = 341 bits (875), Expect = 4e-93, Method: Compositional matrix adjust. Identities = 185/433 (42%), Positives = 269/433 (62%), Gaps = 14/433 (3%) Query: 383 FSAETAVLHAGYRRDPVTKAVAVPIYQNTAYELDGDLNHIADVYNVKADGFTYTRIINPT 442 + ET +H G+ D T + AVPIYQ T+Y D H A ++ ++ G YTRI+NPT Sbjct: 5 YKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSP-EHAAALFGLQETGNIYTRIMNPT 63 Query: 443 TRALEKRYAAVDMGSDSLAVASGQAATFLAIVNLSSGEVGDNIVASPYLYGNTWNLLHNT 502 T LE+R ++ G ++A ASG AA +I+N++ GD+IVA+ LYG T L +T Sbjct: 64 TAVLEERLTLLEGGIGAVATASGMAAITYSILNIAGS--GDHIVAAATLYGGTHTLFSHT 121 Query: 503 LKRLGISVRTADPRRPETFERAIDDRTICLFGEVISNPCLIPLPVKQLAEIGRKHGVPLV 562 K GI V DP PE FE+A+ + T +F E I NP + + ++++AEI VPL+ Sbjct: 122 FKTFGIDVTFVDPNEPENFEKAMKENTKAVFIETIGNPDINIIDIEKVAEIAHASDVPLI 181 Query: 563 VDNT-TTPLVCRPSDLGAAITTYSATKYICGHGTTLGGLIVDNGKFSYRGASRFPLFNSP 621 VDNT T + RP D GA I YSATK+I GHG +GG ++D+GKF++ +FP P Sbjct: 182 VDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANG-KFPKLVVP 240 Query: 622 DEAHGGIIWRNALQDVDDLGKSEVLLKARMTWLRDTGAAIAPFASFQLIQGLETLPLRMK 681 D+++ G+ + N D+G + + K R++ LRDTGAA++PF +F LI GLETL LR++ Sbjct: 241 DDSYNGLSYTN------DVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLE 294 Query: 682 QHCANARIVADVLKEHPKVRRVFYPGLFEGADRETVDQTLNPAYGHGAMVMFEVE-DEQA 740 QH NA+ VA+ L +HPKV V YPGL + + + L G G++ F V+ A Sbjct: 295 QHVKNAKQVANFLNDHPKVAWVNYPGLKDNKYHDLAQKYL--PKGPGSIFTFGVKGGYAA 352 Query: 741 GRKFIQNVDLMYHVSNVGDARTLVTHPVSTTHTTVPREKREAAGIFGGSIRLCVGIEDVD 800 G+K I++V+L H++NVGDA++L+ HP STTH + E++ AG+ SIRL +GIE+ D Sbjct: 353 GKKVIESVELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPESIRLSIGIENAD 412 Query: 801 DIVRDLDRALSAI 813 DI++DL +AL I Sbjct: 413 DIIQDLTKALEQI 425 >mlr8465 Non COG Length = 426 Score = 339 bits (869), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 184/430 (42%), Positives = 264/430 (61%), Gaps = 13/430 (3%) Query: 386 ETAVLHAGYRRDPVTKAVAVPIYQNTAYELDGDLNHIADVYNVKADGFTYTRIINPTTRA 445 T +HAG + DP T A A PIYQ T++ D D +H A ++ +KA G YTRI+NPT Sbjct: 8 NTLAVHAGAKPDPATGARATPIYQTTSFVFD-DADHAASLFGLKAFGNIYTRIMNPTQAV 66 Query: 446 LEKRYAAVDMGSDSLAVASGQAATFLAIVNLSSGEVGDNIVASPYLYGNTWNLLHNTLKR 505 LE+R AA++ G+ +LAVASG AA + NL + GDN VA+ LYG + N + K Sbjct: 67 LEERVAALEGGTAALAVASGHAAQVIVFHNLM--QPGDNFVAATRLYGGSINQFGHAFKN 124 Query: 506 LGISVRTADPRRPETFERAIDDRTICLFGEVISNPCLIPLPVKQLAEIGRKHGVPLVVDN 565 G VR AD TFE IDDRT +F E ++NP + ++++ +I RKHG+PL+VDN Sbjct: 125 YGWEVRWADTNDLSTFESQIDDRTKAIFIESLANPGGTFVDIEKIGDIARKHGLPLIVDN 184 Query: 566 T-TTPLVCRPSDLGAAITTYSATKYICGHGTTLGGLIVDNGKFSYRGASRFPLFNSPDEA 624 T +P + RP + GA I +S TK+I GHG ++GG+IVD G F + + ++P+ + P Sbjct: 185 TLASPYLIRPIEHGADIVVHSLTKFIGGHGNSIGGVIVDGGTFDWSKSGKYPMLSEPRPE 244 Query: 625 HGGIIWRNALQDVDDLGKSEVLLKARMTWLRDTGAAIAPFASFQLIQGLETLPLRMKQHC 684 +GG++ + G + AR+ LRD G AI+PF +F ++ GLETLPLRM++HC Sbjct: 245 YGGLVLH------ETFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGLETLPLRMQRHC 298 Query: 685 ANARIVADVLKEHPKVRRVFYPGLFEGADRETVDQTLNPAYGHGAMVMFEVED-EQAGRK 743 NA VA L HPKV V YPGL +D+ Q G GA+ F ++ +AG K Sbjct: 299 DNAVTVAGWLSNHPKVAWVNYPGL--PSDKNNALQKKYSPLGAGAVFTFGLKGGYEAGVK 356 Query: 744 FIQNVDLMYHVSNVGDARTLVTHPVSTTHTTVPREKREAAGIFGGSIRLCVGIEDVDDIV 803 F++ ++L H++NVGD ++LV HP STTH + E++ AG ++RL +GIEDV DIV Sbjct: 357 FVEALELFSHLANVGDTKSLVIHPASTTHRQLSDEQKVKAGAGPDTVRLSIGIEDVTDIV 416 Query: 804 RDLDRALSAI 813 DL++AL+ + Sbjct: 417 ADLEQALAKV 426 >PA5025 [E] COG2873 O-acetylhomoserine sulfhydrylase Pae Pseudomonas aeruginosa Length = 425 Score = 337 bits (865), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 188/422 (44%), Positives = 265/422 (62%), Gaps = 18/422 (4%) Query: 386 ETAVLHAGYRRDPVTKAVAVPIYQNTAYELDGDLNHIADVYNVKADGFTYTRIINPTTRA 445 ET +HAGY DP T+AVAVPIYQ T+Y D D H AD++++K G YTRI+NPT Sbjct: 4 ETLAVHAGYSPDPTTRAVAVPIYQTTSYAFD-DTQHGADLFDLKVPGNIYTRIMNPTNDV 62 Query: 446 LEKRYAAVDMGSDSLAVASGQAATFLAIVNLSSGEVGDNIVASPYLYGNTWNLLHNTLKR 505 LE+R AA++ G +LAVASG AA AI ++ E GDNIV+ LYG T+NLL +TL R Sbjct: 63 LEQRVAALEGGVGALAVASGMAAITYAIQTVA--EAGDNIVSVAKLYGGTYNLLAHTLPR 120 Query: 506 LGISVRTADPRRPETFERAIDDRTICLFGEVISNPCLIPLPVKQLAEIGRKHGVPLVVDN 565 +GI R A E ID+RT +F E I NP + ++ LA+ +HGVPL+VDN Sbjct: 121 IGIQARFAAHDDVAALEALIDERTKAVFCETIGNPAGNIIDLQALADAAHRHGVPLIVDN 180 Query: 566 TT-TPLVCRPSDLGAAITTYSATKYICGHGTTLGGLIVDNGKFSYRG-ASRFPLFNSPDE 623 T TP++CRP + GA I +S TKY+ GHGT++GG++VD+GKF + SRFPL N+PD Sbjct: 181 TVATPVLCRPFEHGADIVVHSLTKYMGGHGTSIGGIVVDSGKFDWAANKSRFPLLNTPDP 240 Query: 624 AHGGIIWRNALQDVDDLGKSEVLLKARMTWLRDTGAAIAPFASFQLIQGLETLPLRMKQH 683 ++ G+ + A G + + + R+ LR+ GAA++PF +F ++QGLETL LRM++H Sbjct: 241 SYHGVTYTEA------FGPAAFIGRCRVVPLRNMGAALSPFNAFLILQGLETLALRMERH 294 Query: 684 CANARIVADVLKEHPKVRRVFYPGLFEGADRETVDQTLN--PAYGHGAMVMFEVE-DEQA 740 C NA VA L++HP+V V Y GL + + + L PA A++ F ++ A Sbjct: 295 CDNALAVARYLQQHPQVAWVKYAGLADNPEHALARRYLGGRPA----AILSFGIQGGSAA 350 Query: 741 GRKFIQNVDLMYHVSNVGDARTLVTHPVSTTHTTVPREKREAAGIFGGSIRLCVGIEDVD 800 G +FI + L+ + N+GDA++L HP STTH + E+ AG+ +RL +GIE +D Sbjct: 351 GARFIDALKLVVRLVNIGDAKSLACHPASTTHRQLNAEELARAGVSDDMVRLSIGIEHID 410 Query: 801 DI 802 DI Sbjct: 411 DI 412