BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0181.0008.p (385 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM4376 [E] COG0754 Glutathionylspermidine synthase Sty Salmonel... 384 e-106 YPO0660 [E] COG0754 Glutathionylspermidine synthase Ype Yersinia... 384 e-106 ZyjfC [E] COG0754 Glutathionylspermidine synthase EcZ Escherichi... 383 e-106 ECs5162 [E] COG0754 Glutathionylspermidine synthase Ecs Escheric... 383 e-106 yjfC [E] COG0754 Glutathionylspermidine synthase Eco Escherichia... 382 e-106 >STM4376 [E] COG0754 Glutathionylspermidine synthase Sty Salmonella typhimurium LT2 Length = 387 Score = 384 bits (987), Expect = e-106, Method: Compositional matrix adjust. Identities = 195/385 (50%), Positives = 242/385 (62%), Gaps = 1/385 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M R P R D A + GF FH ID E YWDE Y FTL +IE IE PT E+ MC Sbjct: 1 MLRHNVPIRRDLDKIACDHGFDFHVIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 LE+ RAVRDE+ +++L IP +WD+IA SWR ++PSLYGR+D + + P KLLEYNAD Sbjct: 61 LEIVERAVRDEQSMQQLAIPPLYWDVIAESWRSRDPSLYGRMDFVWCGKDPVKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTS++E++ FQW WLE A + IP+ ADQ+N+I +RLI + + + PL+ Sbjct: 121 TPTSLYESSYFQWLWLEDARRSGAIPRDADQYNAIQERLIARFSELYSREPLYFCCCEDT 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 ED T+ YL+D A QAG +T + IEE+GL G DLD+ I AFKLYP EWM D Sbjct: 181 DEDRTTVLYLQDCAQQAGQETRFIYIEELGLGVGGVLTDLDDNVIRRAFKLYPLEWMMRD 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 G L QWIEP WK+ILSNKG+LPLLW FP HPNLLPA+FE + A G S V Sbjct: 241 DNGPLLRKRREQWIEPLWKSILSNKGLLPLLWRFFPGHPNLLPAWFEGEKSLAAPGESSV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAE-QGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 RKPIYSREG NV + +I G Y E I QA PLP F Y ++GSW++D Sbjct: 301 RKPIYSREGGNVTIFDERQNVIDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIIDDE 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAI 384 G+ IRED IT +TSRF+PH I Sbjct: 361 ASGMGIREDNTLITKDTSRFVPHYI 385 >YPO0660 [E] COG0754 Glutathionylspermidine synthase Ype Yersinia pestis Length = 386 Score = 384 bits (987), Expect = e-106, Method: Compositional matrix adjust. Identities = 192/388 (49%), Positives = 249/388 (64%), Gaps = 6/388 (1%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M+R+A ER DWR A GF FHT+ GE YW E AYY FTL +IE E T E+ MC Sbjct: 1 MKRLAISERPDWREKATEFGFRFHTMHGEPYWCEDAYYEFTLAQIEELEET-TAELHQMC 59 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 L++ + + ++ L + +IP W+ + SWR PSLY RLDL +D + PAKLLE NAD Sbjct: 60 LQVVEKVINSDQLLTKCRIPKHCWEFVRHSWRSNQPSLYSRLDLAYDGKNPAKLLENNAD 119 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHP---LHLTGL 177 TPTS++EAA FQW WLE I + +DQ+NS+ ++LIE + + H LHL Sbjct: 120 TPTSLYEAAFFQWLWLEDQISAGNLSTDSDQYNSLQEKLIERFVELRDNHGFSLLHLACC 179 Query: 178 TGNPEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWM 237 + ED GT+ YL+D A +AGL T + +EEIGL + GQF DLD + I+ FKLYPWE+M Sbjct: 180 QDSEEDRGTVQYLQDCALEAGLPTEFMFMEEIGLGEKGQFTDLDNQVISNLFKLYPWEFM 239 Query: 238 FHDAFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGT 297 F + F L A +W+EP WK+I+SNK +LPLLWEMFPNHPNLLPAYF +D Sbjct: 240 FREIFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPPL--D 297 Query: 298 SFVRKPIYSREGANVELVSAGVTLIAEQGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVD 357 +V KP++SREGAN+++V G + GPYG EG I Q PLP F Y ++GSWLV+ Sbjct: 298 HYVIKPLFSREGANIQIVENGKEVAKVDGPYGEEGMIIQQFHPLPKFGDSYTLIGSWLVN 357 Query: 358 GTPCGLSIREDENPITGNTSRFLPHAIL 385 PCG+ +RED IT + SRF PH IL Sbjct: 358 DQPCGIGLREDRELITQDLSRFYPHIIL 385 >ZyjfC [E] COG0754 Glutathionylspermidine synthase EcZ Escherichia coli O157:H7 EDL933 Length = 387 Score = 383 bits (984), Expect = e-106, Method: Compositional matrix adjust. Identities = 190/385 (49%), Positives = 239/385 (62%), Gaps = 1/385 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M R P R D A + GF FH ID E YWDE Y FTL +IE IE PT E+ MC Sbjct: 1 MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 LE+ RAV+DE L +L IP +WD+IA SWR ++PSLYGR+D + P KLLEYNAD Sbjct: 61 LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTS++E+A FQW WLE A + IIP+ ADQ+N+I +RLI + + + P + Sbjct: 121 TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 ED T+ YL+D A QAG ++ + IE++GL G DLD+ I AFKLYP EWM D Sbjct: 181 DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 G L QW+EP WK+ILSNKG++PLLW FP HPNLL ++FE + G S+V Sbjct: 241 DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAE-QGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 RKPIYSREG NV + ++ G Y E I QA PLP F Y ++GSW+VD Sbjct: 301 RKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAI 384 CG+ IRED IT +TSRF+PH I Sbjct: 361 ACGMGIREDNTLITKDTSRFVPHYI 385 >ECs5162 [E] COG0754 Glutathionylspermidine synthase Ecs Escherichia coli O157:H7 Length = 387 Score = 383 bits (984), Expect = e-106, Method: Compositional matrix adjust. Identities = 190/385 (49%), Positives = 239/385 (62%), Gaps = 1/385 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M R P R D A + GF FH ID E YWDE Y FTL +IE IE PT E+ MC Sbjct: 1 MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 LE+ RAV+DE L +L IP +WD+IA SWR ++PSLYGR+D + P KLLEYNAD Sbjct: 61 LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTS++E+A FQW WLE A + IIP+ ADQ+N+I +RLI + + + P + Sbjct: 121 TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 ED T+ YL+D A QAG ++ + IE++GL G DLD+ I AFKLYP EWM D Sbjct: 181 DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 G L QW+EP WK+ILSNKG++PLLW FP HPNLL ++FE + G S+V Sbjct: 241 DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAE-QGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 RKPIYSREG NV + ++ G Y E I QA PLP F Y ++GSW+VD Sbjct: 301 RKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAI 384 CG+ IRED IT +TSRF+PH I Sbjct: 361 ACGMGIREDNTLITKDTSRFVPHYI 385 >yjfC [E] COG0754 Glutathionylspermidine synthase Eco Escherichia coli K12 Length = 387 Score = 382 bits (980), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/385 (49%), Positives = 239/385 (62%), Gaps = 1/385 (0%) Query: 1 MQRIACPERDDWRTTAENAGFTFHTIDGERYWDERAYYAFTLDEIERSIEAPTGEIDAMC 60 M R P R D A + GF FH ID E YWDE Y FTL +IE IE PT E+ MC Sbjct: 1 MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC 60 Query: 61 LELAGRAVRDERYLRRLKIPDAFWDLIAASWRRKNPSLYGRLDLTFDARGPAKLLEYNAD 120 LE+ RAV+DE L +L IP +WD+IA SWR ++PSLYGR+D + P KLLEYNAD Sbjct: 61 LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD 120 Query: 121 TPTSIFEAAVFQWTWLEQAIQRNIIPKRADQFNSIHDRLIEAWKRIGATHPLHLTGLTGN 180 TPTS++E+A FQW WLE A + IIP+ ADQ+N+I +RLI + + + P + Sbjct: 121 TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT 180 Query: 181 PEDAGTLAYLKDTAAQAGLKTTLLDIEEIGLRDDGQFVDLDERPIALAFKLYPWEWMFHD 240 ED T+ YL+D A QAG ++ + IE++GL G DLD+ I AFKLYP EWM D Sbjct: 181 DEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD 240 Query: 241 AFGKNLSSAPTQWIEPPWKAILSNKGILPLLWEMFPNHPNLLPAYFEDDPQAAQLGTSFV 300 G L QW+EP WK+ILSNKG++PLLW FP HPNLL ++F+ + G S+V Sbjct: 241 DNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFDGEKPQIAAGESYV 300 Query: 301 RKPIYSREGANVELVSAGVTLIAE-QGPYGTEGFIRQALAPLPNFSGQYPVLGSWLVDGT 359 RKPIYSREG NV + ++ G Y E I QA PLP F Y ++GSW+VD Sbjct: 301 RKPIYSREGGNVTIFDGKNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE 360 Query: 360 PCGLSIREDENPITGNTSRFLPHAI 384 CG+ IRED IT +TSRF+PH I Sbjct: 361 ACGMGIREDNTLITKDTSRFVPHYI 385