BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0180.0050.p (262 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00260 [IQR] COG1028 Dehydrogenases with different specificiti... 186 4e-47 AGl126 [IQR] COG1028 Dehydrogenases with different specificities... 186 5e-47 TM0325 [IQR] COG1028 Dehydrogenases with different specificities... 162 6e-40 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 159 5e-39 BH3896 [IQR] COG1028 Dehydrogenases with different specificities... 158 1e-38 >SMc00260 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 254 Score = 186 bits (472), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 112/247 (45%), Positives = 150/247 (60%), Gaps = 2/247 (0%) Query: 7 KVALVTGAARGIGLAAAKRFLGDGWRVALLDIEGELLRAVVAALKQPDDTLALTCDVSDA 66 K L+TG A GIGL A+ GWRV L+D + L A+ D A+ C V+D Sbjct: 6 KSVLITGGASGIGLEIARLLHERGWRVYLMDRNADALADACRAIP-IDPAQAIACSVTDE 64 Query: 67 AGVAAAISAITDRFGRLDALVNNAGVAVFTPVLETSDADWSRIMAVNLTGPFL-CTKAAA 125 V +AI+ L A++N+AG+A+ P +ETS D+ RI+ VNLTG F+ C +AA Sbjct: 65 KEVESAIATAAAAGAPLRAVINSAGIAMDRPAVETSVDDFRRILDVNLTGTFIVCREAAR 124 Query: 126 PVMREHGGGAIVNITSISAVRASTLRSAYGTSKAGLAHLTKQLAVELASLGIRVNGVAPG 185 + GAIVNI+S+S + + R+AYG SK + LT LA EL GIRVN +APG Sbjct: 125 HWLATATPGAIVNISSVSGLVGNKGRAAYGASKGAVNLLTYILATELGQDGIRVNAIAPG 184 Query: 186 PVDTAMAKAVHTPEIRADYHDAIPLNRYGLEEELAEAIFFLCSDRASYITGQILAVDGGF 245 +DT +++AVHT ++RA +H+ IP RYG E+A + FL S+ ASYI GQILAVDGGF Sbjct: 185 AIDTPLSRAVHTDDVRAQWHERIPQRRYGSSREIAASAAFLISEEASYINGQILAVDGGF 244 Query: 246 DAAGFGL 252 AG L Sbjct: 245 VNAGLAL 251 >AGl126 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 259 Score = 186 bits (472), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 108/247 (43%), Positives = 150/247 (60%), Gaps = 6/247 (2%) Query: 11 VTGAARGIGLAAAKRFLGDGWRVALLDIEGELLRAVVAALKQPDDTLALTCDVSDAAGVA 70 +TG A GIG + A+ + GW+ LLD++ E L L D + CDV+D A + Sbjct: 14 ITGGASGIGFSTAQLLMARGWQPWLLDLKREALDIACEKLG-IDLWRGIVCDVADEASIE 72 Query: 71 AAISAIT----DRFGRLDALVNNAGVAVFTPVLETSDADWSRIMAVNLTGPFLCTKAAAP 126 A +A+T D L A+VN+AG+ + ++TS ++ RI+ VNL G F +AAA Sbjct: 73 MAFAALTANGADHAVDLVAVVNSAGIGIDKLSVDTSVEEFRRIVDVNLVGSFAVARAAAR 132 Query: 127 VMREHG-GGAIVNITSISAVRASTLRSAYGTSKAGLAHLTKQLAVELASLGIRVNGVAPG 185 E G+IVNI+S+S + + RSAYG SK G+ LT +A EL GIRVN +APG Sbjct: 133 YWLERDVAGSIVNISSVSGMCGNRGRSAYGASKGGVNLLTMVMANELGQSGIRVNAIAPG 192 Query: 186 PVDTAMAKAVHTPEIRADYHDAIPLNRYGLEEELAEAIFFLCSDRASYITGQILAVDGGF 245 PVDT + +AVHT +R +H +P++RYG +E+A A+ FL SD ASYI GQ+LAVDGGF Sbjct: 193 PVDTPLTQAVHTENVRDQWHSRVPMHRYGRTDEIASAVAFLVSDDASYINGQVLAVDGGF 252 Query: 246 DAAGFGL 252 AG + Sbjct: 253 ITAGLAV 259 >TM0325 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 251 Score = 162 bits (411), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 101/244 (41%), Positives = 133/244 (54%), Gaps = 8/244 (3%) Query: 7 KVALVTGAARGIGLAAAKRFLGDGWRVALLDIEGELLRAVVAALKQPDDTLALTCDVSDA 66 KV L+TGA GIG AA F G +VA+ DI E + V +K A D Sbjct: 6 KVVLITGAGSGIGKKAAVMFAERGAKVAINDISEEKGKETVELIKSMGGEAAFI--FGDV 63 Query: 67 AGVAAAISAIT-DRFGRLDALVNNAGVAVFTPVLETSDADWSRIMAVNLTGPFLCTKAAA 125 A A I T + FGRLD LVNNAG+ + + ETS+ D+ + MAVN+ GPFL +K A Sbjct: 64 AKDAEQIVKKTVETFGRLDILVNNAGIVPYGNIEETSEEDFDKTMAVNVKGPFLLSKYAV 123 Query: 126 PVMREHGGGAIVNITSISAVRASTLRSAYGTSKAGLAHLTKQLAVELASLGIRVNGVAPG 185 M++ GGG IVN++S + + R Y SKA L LT+ LAV+ GIRVN V PG Sbjct: 124 EQMKKQGGGVIVNVSSEAGLIGIPRRCVYSVSKAALLGLTRSLAVDYVDYGIRVNAVCPG 183 Query: 186 PVDTA--MAKAVHTP---EIRADYHDAIPLNRYGLEEELAEAIFFLCSDRASYITGQILA 240 + MA+ +P E+ IP+ R G EEE+A AI F D A ++TG I+ Sbjct: 184 TTQSEGLMARVKASPNPEELLKKMTSRIPMKRLGKEEEIAFAILFAACDEAGFMTGSIIN 243 Query: 241 VDGG 244 +DGG Sbjct: 244 IDGG 247 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 246 Score = 159 bits (403), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 92/241 (38%), Positives = 134/241 (55%), Gaps = 4/241 (1%) Query: 7 KVALVTGAARGIGLAAAKRFLGDGWRVALLDIEGELLRAVVA-ALKQPDDTLALTCDVSD 65 KV L+TGAA GIG A F +G V DI E L ++V A P +V+D Sbjct: 6 KVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYVLNVTD 65 Query: 66 AAGVAAAISAITDRFGRLDALVNNAGVAVFTPVLETSDADWSRIMAVNLTGPFLCTKAAA 125 + + + ++GR+D LVNNAG+ ++ + DW ++ VNL G F T+ Sbjct: 66 RDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNVTQMVV 125 Query: 126 PVMREHGGGAIVNITSISAVRASTLRSAYGTSKAGLAHLTKQLAVELASLGIRVNGVAPG 185 P M + G+IVN++S+ + + ++ Y SKAG+ +TK A ELA IRVN VAPG Sbjct: 126 PYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAVAPG 185 Query: 186 PVDTAMAKAVHTPE-IRADYHDAIPLNRYGLEEELAEAIFFLCSDRASYITGQILAVDGG 244 ++T M + + PE R IPL R+G EE+A+ I FL SD +SY+TGQ++ +DGG Sbjct: 186 FIETPMTEKL--PEKARETALSRIPLGRFGKPEEVAQVILFLASDESSYVTGQVIGIDGG 243 Query: 245 F 245 Sbjct: 244 L 244 >BH3896 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 158 bits (399), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 91/239 (38%), Positives = 133/239 (55%), Gaps = 2/239 (0%) Query: 7 KVALVTGAARGIGLAAAKRFLGDGWRVALLDIEGELLRAVVAALKQPD-DTLALTCDVSD 65 KVA++TGA RGIG A AK+F +G +V + D+ E + VA ++ + L DV+ Sbjct: 6 KVAMITGAGRGIGAATAKKFAREGAKVIVCDVREEEVAKTVAEIQDGGGEALGSVVDVTQ 65 Query: 66 AAGVAAAISAITDRFGRLDALVNNAGVAVFTPVLETSDADWSRIMAVNLTGPFLCTKAAA 125 V I+ + +RF LD +VNNAG+ + +DA W ++ VNL G F+ T+ Sbjct: 66 RKDVKNVINQVIERFETLDVVVNNAGITADAQLTNMTDAQWDDVIDVNLKGVFIVTQEVT 125 Query: 126 PVMREHGGGAIVNITSISAVRASTLRSAYGTSKAGLAHLTKQLAVELASLGIRVNGVAPG 185 +M+E G I+N +S+ + ++ Y SK G+ +TK A EL IRVN VAPG Sbjct: 126 TIMKEQKRGVILNASSVVGSYGNFGQTNYAASKWGVNGMTKTWAKELGRYNIRVNAVAPG 185 Query: 186 PVDTAMAKAVHTPEIRADYHDAIPLNRYGLEEELAEAIFFLCSDRASYITGQILAVDGG 244 + T M + + ++ A+ LNR G EE+A FL SD AS+ITG ILA+DGG Sbjct: 186 FILTPMTEKMPEKVLKVMEEKAV-LNRLGTVEEVANGYAFLASDEASFITGTILAIDGG 243