BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0180.0005.p (413 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MJ1465 [E] COG0498 Threonine synthase Mja Methanococcus jannaschii 217 4e-56 VNG0541G [E] COG0498 Threonine synthase Hbs Halobacterium sp. NRC-1 215 1e-55 aq_608 [E] COG0498 Threonine synthase Aae Aquifex aeolicus 199 1e-50 MA1610 [E] COG0498 Threonine synthase Mac Methanosarcina acetivo... 198 2e-50 MK0974 [E] COG0498 Threonine synthase Mka Methanopyrus kandleri ... 190 7e-48 >MJ1465 [E] COG0498 Threonine synthase Mja Methanococcus jannaschii Length = 405 Score = 217 bits (553), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 127/377 (33%), Positives = 198/377 (52%), Gaps = 5/377 (1%) Query: 20 CAMEGDHYPADQVHNLSKAGKPLLVRYDLAGVKKALTKDALAQRPADLWRYRELLPVRKC 79 C G Y D++ + G L + YD +K ++++ L +R +WRY E LPV+ Sbjct: 5 CIKCGKTYDVDEIIYTCECGGLLEIIYDYEEIKDKVSEEKLRKREIGVWRYLEYLPVKDE 64 Query: 80 QDIVSLGEVTTPLIRLPRLGARLGGGEIIVKDEGRLPTGSFKARGLVMAVSMGKALGIKH 139 IVSL E TPL R L LG E+ VK+EG PTGSFK RG+ + V+ LG++ Sbjct: 65 SKIVSLCEGGTPLYRCNNLEKELGIKELYVKNEGANPTGSFKDRGMTVGVTRANELGVEV 124 Query: 140 MAMPTNGNAGAALAAYATSCGIKTTIFCPADTPEV-NVSEIELQGATVYRVNGYIDDCGK 198 + + GN A+LAAY+ G K + P + +++ GA V +V G DD Sbjct: 125 VGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGNFDDALD 184 Query: 199 IVGE-GKAKVGWFDTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGLIGMW 257 +V + K K+ + S P+R+EG+KT+ E+ +QL W VPD + P G + +W Sbjct: 185 MVKQLAKEKLIYLLNSI--NPFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGNISAIW 242 Query: 258 KAFDELEKIGFIGSKRPRMVAVQASGCAPMVRAYEAGTEHAARWEDAHTIASGIRVPQAI 317 K F E E G I + P+M +QA G P+V A+ + +++ TIA+ IR+ + Sbjct: 243 KGFKEFEITGII-DELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIATAIRIGNPV 301 Query: 318 GDFLILRAVRESKGFAIAVDDDKISAALNEVAREEGLLLCPEGAATYAAYKESLADGRVS 377 L A+ S G+A AV D++I A +AR+EG+ + P A++ A K+ L +G + Sbjct: 302 NAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLEEGIID 361 Query: 378 KTDRVMLFNCASGLKYP 394 + +R++ GLK P Sbjct: 362 RDERIVCITTGHGLKDP 378 >VNG0541G [E] COG0498 Threonine synthase Hbs Halobacterium sp. NRC-1 Length = 398 Score = 215 bits (548), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 132/342 (38%), Positives = 180/342 (52%), Gaps = 12/342 (3%) Query: 18 LECAMEGDHYPADQ-VHNLSKAGKPLLVRYDLAGVKKALTKDAL---AQRPADLWRYREL 73 LEC G+ + A H + G L YDL + L DA A+R LWRY EL Sbjct: 10 LECVDCGESFAAAAATHRCPECGGILDPAYDL----ENLDVDAETFGARRFDSLWRYAEL 65 Query: 74 LPVRKCQDIVSLGEVTTPLIRLPRLGARLGGGEIIVKDEGRLPTGSFKARGLVMAVSMGK 133 LP + VS+ E T L+ P L LG GE+ +KDEGR PTG+F+ RG +A++ Sbjct: 66 LPFTRGS-AVSMDEGATALVPCPGLADELGVGELYIKDEGRNPTGTFRDRGQTVAMTAAA 124 Query: 134 ALGIKHMAMPTNGNAGAALAAYATSCGIKTTIFCPADTPEVNVSEIELQGATVYRVNGYI 193 G +A+ + GNAG + AYA G+ +F P+ + + + + G + V G I Sbjct: 125 QHGASEVALASTGNAGQSAGAYAGRAGLDAHVFLPSRSGFTTKAMVNVHGGDMNVVQGRI 184 Query: 194 DDCGKIVGEGKAKVG--WFDTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGT 251 D + + G W+ PYR EGKKT+ E EQL W+ PD + YPTG G Sbjct: 185 GDANAAFEDAMDEHGEAWYSLQAFATPYRHEGKKTLLYETVEQLDWETPDHVVYPTGDGG 244 Query: 252 GLIGMWKAFDELEKIGFIGSKRPRMVAVQASGCAPMVRAYEAGTEHAARWEDAHTIASGI 311 GL+GMWKA EL ++G I + P M A QA GCAP+VRA++ G + W+ TI GI Sbjct: 245 GLVGMWKAATELRELG-ITADVPAMYAAQAEGCAPIVRAFQEGRDAHEPWDTPDTICGGI 303 Query: 312 RVPQAIGDFLILRAVRESKGFAIAVDDDKISAALNEVAREEG 353 +P G L+L A+RES G A+A D++I A VA G Sbjct: 304 EIPDPAGSRLVLEALRESNGGAVAASDEEILEAGTAVASRAG 345 >aq_608 [E] COG0498 Threonine synthase Aae Aquifex aeolicus Length = 409 Score = 199 bits (505), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 121/379 (31%), Positives = 186/379 (49%), Gaps = 4/379 (1%) Query: 15 VTHLECAMEGDHYPADQVHNLSKAGKPLLVRYDLAGVKKALTKDALAQRPADLWRYRELL 74 V L+C G YPA+ +H PL V YD +KK ++++ + + P LWRY +LL Sbjct: 4 VKGLKCRECGREYPAEPIHVCEFCFGPLEVVYDYDEIKKNISREKIEKGPKSLWRYVDLL 63 Query: 75 PVRKCQDIVSLGEVTTPLIRLPRLGARLGGGEIIVKDEG-RLPTGSFKARGLVMAVSMGK 133 PV V L TPL + +LG LG + +KD+ PT SFK R + +A+S K Sbjct: 64 PVE--NPTVGLTAGFTPLKKAEKLGEVLGLKNLYIKDDSVNHPTLSFKDRVVSVALSKAK 121 Query: 134 ALGIKHMAMPTNGNAGAALAAYATSCGIKTTIFCPADTPEVNVSEIELQGATVYRVNGYI 193 G A + GN ++AA++ G+ +F PA+ + + TV V G Sbjct: 122 EFGFDTAACASTGNLANSVAAHSAQAGMNCFVFIPANLETQKIIGSLVFNPTVVAVEGNY 181 Query: 194 DDCGKIVGEGKAKVGWFDTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGL 253 DD ++ E +GW + PY EG KT+ E+AEQLGW PDV+ P G+ Sbjct: 182 DDVNRLCSEIANDLGWAFVNINIRPYYAEGSKTLAFEVAEQLGWRAPDVVVAPAASGSLY 241 Query: 254 IGMWKAFDELEKIGFIGSKRPRMVAVQASGCAPMVRAYEAGTEHAARWEDAHTIASGIRV 313 +WK F+EL+K+G I + RM QA GC+P+ +A+ G + + +TIA I + Sbjct: 242 TKIWKGFNELKKVGLIDEVKTRMYGAQAEGCSPIAQAWREGRDF-IKPVKPNTIAKSIAI 300 Query: 314 PQAIGDFLILRAVRESKGFAIAVDDDKISAALNEVAREEGLLLCPEGAATYAAYKESLAD 373 L+ RES G D++I + +A+ EG+ G T A K+ + Sbjct: 301 GNPADGIYALQVARESNGAWETATDEEIIEGIKLLAQTEGIFTETAGGTTIAVLKKYVEK 360 Query: 374 GRVSKTDRVMLFNCASGLK 392 G +S + V+++ +G K Sbjct: 361 GMISPEETVVVYITGNGYK 379 >MA1610 [E] COG0498 Threonine synthase Mac Methanosarcina acetivorans str.C2A Length = 405 Score = 198 bits (503), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 127/380 (33%), Positives = 202/380 (53%), Gaps = 8/380 (2%) Query: 17 HLECAMEGDHYPADQV-HNLSKAGKPLLVRYDLAGVKKALTKDALAQRPADLWRYRELLP 75 HL+C G Y D+V + SK L V YD + +K + K L +W+Y +LLP Sbjct: 3 HLKCIECGAEYSRDEVIYTCSKCDGLLDVIYDYSSIKIDMEK--LKTECPSVWKYAKLLP 60 Query: 76 VRKCQDIVSLGEVTTPLIRLPRLGARLGGGEIIVKDEGRLPTGSFKARGLVMAVSMGKAL 135 V + + V++ E TPL + RL ++G ++ VK EG PTGSFK RG+ + V+ L Sbjct: 61 VER--EPVTIQEGGTPLYKCDRLAEKIGIKKLYVKHEGMNPTGSFKDRGMTVGVTKALEL 118 Query: 136 GIKHMAMPTNGNAGAALAAYATSCGIKTTIFCPADTPEV-NVSEIELQGATVYRVNGYID 194 G+ +A + GN AALA Y GI + PA + V++ + GA V + G D Sbjct: 119 GMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKVLSIRGNFD 178 Query: 195 DCGKIVGEGKAKVGWFDTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGLI 254 D +V ++ + +++ PYR+EG+KT+G E+A+QL + VPD I P G + Sbjct: 179 DALALVRTLCSQEKIYLLNSIN-PYRLEGQKTIGFEIADQLDFKVPDRIVLPVGNAGNIT 237 Query: 255 GMWKAFDELEKIGFIGSKRPRMVAVQASGCAPMVRAYEAGTEHAARWEDAHTIASGIRVP 314 ++K F E + +G S P+M +QA G P+V+A ++G E+ T+A+ IR+ Sbjct: 238 AIYKGFREFKILGITDS-LPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVATAIRIG 296 Query: 315 QAIGDFLILRAVRESKGFAIAVDDDKISAALNEVAREEGLLLCPEGAATYAAYKESLADG 374 + L A+RES G A +V D++I AA ++AR EG+ + P AA+ A ++ + G Sbjct: 297 NPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLRKLVDMG 356 Query: 375 RVSKTDRVMLFNCASGLKYP 394 + + + V+ LK P Sbjct: 357 VIGRDETVVCITTGHLLKDP 376 >MK0974 [E] COG0498 Threonine synthase Mka Methanopyrus kandleri AV19 Length = 403 Score = 190 bits (482), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 129/380 (33%), Positives = 195/380 (51%), Gaps = 10/380 (2%) Query: 18 LECAMEGDHYPADQVHNLSKAGKPLLV--RYDLAGVKKALTKDALAQRPADLWRYRELLP 75 LEC G + D + + G L V RYD + +++ R +W +RELLP Sbjct: 5 LECVECGSEFDDDSLVRCPECGGLLEVALRYD----EIEVSRSTFEGRRLGVWAFRELLP 60 Query: 76 VRKCQDIVSLGEVTTPLIRLPRLGARLGGGEIIVKDEGRLPTGSFKARGLVMAVSMGKAL 135 V D V++ E T L PRLG LG E+ VK+EG PTGSFK RG+ + V+ L Sbjct: 61 VNT-DDPVTMSEGGTGLHPCPRLGEELGVRELFVKNEGENPTGSFKDRGMTVGVTKAVEL 119 Query: 136 GIKHMAMPTNGNAGAALAAYATSCGIKTTIFCPADTPEV-NVSEIELQGATVYRVNGYID 194 G + +A + GN A+LAAYA G+ + PA + +++ GA V VNG D Sbjct: 120 GAEVVACASTGNTSASLAAYAARAGLTCAVLLPAGKVALGKLAQALFHGARVIPVNGNFD 179 Query: 195 DCGKIVGEGKAKVGWFDTSTLKEPYRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGLI 254 D +V + A G P+R+ G++T+ E+ QL W+VPD + P G + Sbjct: 180 DALDVVKK-LADEGIVYILNSVNPFRLSGQRTIAYEICLQLDWEVPDAVVVPVGNAGNIS 238 Query: 255 GMWKAFDELEKIGFIGSKRPRMVAVQASGCAPMVRAYEAGTEHAARWEDAHTIASGIRVP 314 +W+ F +L ++G I + PRM VQA G P+V A G E + T+A+ IR+ Sbjct: 239 AIWQGFLDLYRLGII-DELPRMYGVQAEGARPIVEAIRRGKEDIEPDPEPETVATAIRIG 297 Query: 315 QAIGDFLILRAVRESKGFAIAVDDDKISAALNEVAREEGLLLCPEGAATYAAYKESLADG 374 + LRA+R S G+A+ V D++I +A E+A EG+ + P AA+ A ++ + +G Sbjct: 298 NPVNAIKALRAIRCSGGWAVEVSDEEILSAQRELATREGIFVEPASAASIAGLRKFVEEG 357 Query: 375 RVSKTDRVMLFNCASGLKYP 394 + +RV+ GLK P Sbjct: 358 EIDADERVVCVTTGHGLKDP 377