BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0169.0008.p (263 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20073 [IQR] COG1028 Dehydrogenases with different specificiti... 427 e-120 AGpA398 [IQR] COG1028 Dehydrogenases with different specificitie... 348 6e-96 alr5182 [IQR] COG1028 Dehydrogenases with different specificitie... 296 4e-80 BS_ydaD [IQR] COG1028 Dehydrogenases with different specificitie... 285 5e-77 BS_yhdF [IQR] COG1028 Dehydrogenases with different specificitie... 281 1e-75 >SMb20073 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 285 Score = 427 bits (1099), Expect = e-120, Method: Compositional matrix adjust. Identities = 200/263 (76%), Positives = 229/263 (87%) Query: 1 MDPRPDHGETSYKGSGRLQGKCAIITGGDSGIGRAVAIAFAREGADVLIAYLDESDDAKE 60 M P PDHGE SY+GSGRL+ K AIITGGDSGIGRAVAIA+AREGADVLI+YL E DDA Sbjct: 23 MQPLPDHGENSYQGSGRLKDKRAIITGGDSGIGRAVAIAYAREGADVLISYLSEHDDAMA 82 Query: 61 VAAMIEKEGRRAVLVEGDLRSADHCRTVVDRAVQELGGVDILVNNAAHQATFADIGDISD 120 A++E+ GR+AVL GD++S+DHCR +V+ AV+ELGG+DILVNNAAHQATF +I DISD Sbjct: 83 TKALVEEAGRKAVLAAGDIQSSDHCRRIVETAVRELGGIDILVNNAAHQATFKNIEDISD 142 Query: 121 EEWRMTFEVNIHAMFYLAKAAVPHMKPGGAIINTASVNSDMPNPILLTYATTKGAIQNFT 180 EEW +TF VN+HAMFYL KAAVPHMK G AIINTAS+N+D+PNPILL YATTKGAI NF+ Sbjct: 143 EEWELTFRVNMHAMFYLTKAAVPHMKKGSAIINTASINADVPNPILLAYATTKGAIHNFS 202 Query: 181 GGLAQMLAENGIRVNAVAPGPIWTPLIPSTMPEEAVKNFGKQVPLKRAGQPAELATAYVM 240 GLAQMLAE GIRVN VAPGPIWTPLIPSTMPE+ V +FGKQVP+KR GQP ELA+AYVM Sbjct: 203 AGLAQMLAERGIRVNVVAPGPIWTPLIPSTMPEDTVADFGKQVPMKRPGQPVELASAYVM 262 Query: 241 LADPLSSYTSGTTVAVTGGKPFI 263 LADP+SSY SG T+AVTGGKPF+ Sbjct: 263 LADPMSSYVSGATIAVTGGKPFL 285 >AGpA398 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 348 bits (894), Expect = 6e-96, Method: Compositional matrix adjust. Identities = 167/263 (63%), Positives = 204/263 (77%) Query: 1 MDPRPDHGETSYKGSGRLQGKCAIITGGDSGIGRAVAIAFAREGADVLIAYLDESDDAKE 60 M P PDHGE SY G G+L+G+ A+ITG DSGIG+AVAIAFAREGAD++I+YL+E DDA++ Sbjct: 25 MKPIPDHGEQSYVGDGKLKGRVALITGADSGIGKAVAIAFAREGADIVISYLNEDDDARD 84 Query: 61 VAAMIEKEGRRAVLVEGDLRSADHCRTVVDRAVQELGGVDILVNNAAHQATFADIGDISD 120 A +++ G+ A+LV GD++S +HC+ +V RAV ELG VDILVNNAA Q T+ DI DI+ Sbjct: 85 TAKWVKEAGQGALLVPGDIKSEEHCKNLVHRAVNELGAVDILVNNAAFQRTYDDIADITA 144 Query: 121 EEWRMTFEVNIHAMFYLAKAAVPHMKPGGAIINTASVNSDMPNPILLTYATTKGAIQNFT 180 EEW TF NI+A F+L+KAA PHMKPG +IINT S+ S P+P LL YA+TKGAI NFT Sbjct: 145 EEWDETFRTNIYAPFFLSKAAAPHMKPGSSIINTTSIQSRQPSPQLLAYASTKGAISNFT 204 Query: 181 GGLAQMLAENGIRVNAVAPGPIWTPLIPSTMPEEAVKNFGKQVPLKRAGQPAELATAYVM 240 GLA+MLAE GIRVNAVAPGPIWTPLIPSTMP E FG+ + RAGQPAELA AYV+ Sbjct: 205 AGLAEMLAEKGIRVNAVAPGPIWTPLIPSTMPAEKAAKFGENTLIGRAGQPAELAGAYVL 264 Query: 241 LADPLSSYTSGTTVAVTGGKPFI 263 LA L SY +G + VTGG+ I Sbjct: 265 LASDLGSYMTGAVIPVTGGEIMI 287 >alr5182 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 285 Score = 296 bits (757), Expect = 4e-80, Method: Compositional matrix adjust. Identities = 144/260 (55%), Positives = 181/260 (69%) Query: 1 MDPRPDHGETSYKGSGRLQGKCAIITGGDSGIGRAVAIAFAREGADVLIAYLDESDDAKE 60 M P+P + Y+GSG+L+ K A+ITGGDSGIGRAVAIA+A+EGADV YL E DDA+E Sbjct: 22 MQPKPQADDAQYRGSGKLKDKVALITGGDSGIGRAVAIAYAKEGADVAFVYLSEHDDAEE 81 Query: 61 VAAMIEKEGRRAVLVEGDLRSADHCRTVVDRAVQELGGVDILVNNAAHQATFADIGDISD 120 ++E++GRRAV + GD+ C+ + + V E G +DIL+NNAA Q I DI+ Sbjct: 82 TKNLVEEQGRRAVSIAGDITDEAFCQRAIQQTVDEFGKLDILINNAAEQHPQESIEDITK 141 Query: 121 EEWRMTFEVNIHAMFYLAKAAVPHMKPGGAIINTASVNSDMPNPILLTYATTKGAIQNFT 180 E+ TF NI +MFYL KAA+ H+K G AIINT SV + +P LL Y++TKGAI FT Sbjct: 142 EQLERTFRTNIFSMFYLTKAAIKHLKKGSAIINTTSVTAYKGSPQLLDYSSTKGAIVAFT 201 Query: 181 GGLAQMLAENGIRVNAVAPGPIWTPLIPSTMPEEAVKNFGKQVPLKRAGQPAELATAYVM 240 L+Q L GIRVNAVAPGPIWTPLIPST P E V+ FGKQVP++RAGQP E+A +YV Sbjct: 202 RSLSQNLISKGIRVNAVAPGPIWTPLIPSTFPAEKVETFGKQVPMQRAGQPEEVAPSYVF 261 Query: 241 LADPLSSYTSGTTVAVTGGK 260 LA SSY SG + GG+ Sbjct: 262 LASDDSSYMSGQVLHPNGGE 281 >BS_ydaD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 286 Score = 285 bits (730), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 135/259 (52%), Positives = 179/259 (69%) Query: 1 MDPRPDHGETSYKGSGRLQGKCAIITGGDSGIGRAVAIAFAREGADVLIAYLDESDDAKE 60 M+P P + YKG+ +L+GK A+ITGGDSGIGRAV++A+A+EGAD+ I Y DE +DA+E Sbjct: 23 MNPSPVYEYEDYKGADKLKGKVALITGGDSGIGRAVSVAYAKEGADIAIVYKDEHEDAEE 82 Query: 61 VAAMIEKEGRRAVLVEGDLRSADHCRTVVDRAVQELGGVDILVNNAAHQATFADIGDISD 120 +E+EG + +L+ GD+ + C V++ V+ELGG+DILVNNA Q I DI+ Sbjct: 83 TKKRVEQEGVKCLLIAGDVGEEEFCNEAVEKTVKELGGLDILVNNAGEQHPKESIKDITS 142 Query: 121 EEWRMTFEVNIHAMFYLAKAAVPHMKPGGAIINTASVNSDMPNPILLTYATTKGAIQNFT 180 E+ TF+ N ++ FYL K A+ ++KPG AIINT S+N + NP L+ Y TKGAI FT Sbjct: 143 EQLHRTFKTNFYSQFYLTKKAIDYLKPGSAIINTTSINPYVGNPTLIDYTATKGAINAFT 202 Query: 181 GGLAQMLAENGIRVNAVAPGPIWTPLIPSTMPEEAVKNFGKQVPLKRAGQPAELATAYVM 240 +AQ L ++GIRVNAVAPGPIWTPLIP+T PEE V FG+ P+ R GQP E YV+ Sbjct: 203 RTMAQALVKDGIRVNAVAPGPIWTPLIPATFPEETVAQFGQDTPMGRPGQPVEHVGCYVL 262 Query: 241 LADPLSSYTSGTTVAVTGG 259 LA SSY +G T+ V GG Sbjct: 263 LASDESSYMTGQTLHVNGG 281 >BS_yhdF [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 289 Score = 281 bits (719), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 142/263 (53%), Positives = 180/263 (68%), Gaps = 1/263 (0%) Query: 1 MDPRPDHGETSYKGSGRLQGKCAIITGGDSGIGRAVAIAFAREGADVLIAYLDESDDAKE 60 M+P P + Y+GSG+L+GK AIITGGDSGIGRA AIAFA+EGAD+ I YLDE DA+E Sbjct: 26 MNPLPLSEDEDYRGSGKLKGKVAIITGGDSGIGRAAAIAFAKEGADISILYLDEHSDAEE 85 Query: 61 VAAMIEKEGRRAVLVEGDLRSADHCRTVVDRAVQELGGVDILVNNAAHQATFADIGDISD 120 IEKE R +L+ GD+ +HC V + V G +DILVNNAA Q I +IS Sbjct: 86 TRKRIEKENVRCLLIPGDVGDENHCEQAVQQTVDHFGKLDILVNNAAEQHPQDSILNIST 145 Query: 121 EEWRMTFEVNIHAMFYLAKAAVPHMKPGGAIINTASVNSDMPNPILLTYATTKGAIQNFT 180 E+ TF NI +MF++ K A+PH++ G AIINT S+ + + L+ Y++TKGAI +FT Sbjct: 146 EQLEKTFRTNIFSMFHMTKKALPHLQEGCAIINTTSITAYEGDTALIDYSSTKGAIVSFT 205 Query: 181 GGLAQMLAENGIRVNAVAPGPIWTPLIPSTMPEEAVKNFGKQVPLKRAGQPAELATAYVM 240 +A+ LA+ GIRVNAVAPGPIWTPLIP+T PEE VK G P+ R GQP E A AYV+ Sbjct: 206 RSMAKSLADKGIRVNAVAPGPIWTPLIPATFPEEKVKQHGLDTPMGRPGQPVEHAGAYVL 265 Query: 241 LADPLSSYTSGTTVAVTGGKPFI 263 LA SSY +G T+ V GG+ FI Sbjct: 266 LASDESSYMTGQTIHVNGGR-FI 287