BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0168.0007.p (537 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0687 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 444 e-124 Rv0166 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 276 1e-73 MT0175 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 271 2e-72 BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid... 270 6e-72 Rv3089 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 270 9e-72 >AF0687 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Afu Archaeoglobus fulgidus Length = 549 Score = 444 bits (1143), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/525 (42%), Positives = 325/525 (61%), Gaps = 9/525 (1%) Query: 4 DIAATIQRAREHSIGDLLRRSAKRYPGKTALICG-----EVSWSFAEMDAICNRLGRGLL 58 ++ + R I D LRRS +RYP KTALI E+ +++E+D + N++ LL Sbjct: 17 ELRKRYNKIRRWVIADFLRRSGRRYPEKTALIFNHPDGREIRLTYSELDKMANKVANALL 76 Query: 59 TLGVAKGDRVAVLSRNSHGFAALRFAVARIGAILVPINFMLNPDEINFILANSGAKLLAV 118 LG+ K DRVA+L+ N+ F A+ GAI + +N+++ ++ + + +S AK+L V Sbjct: 77 NLGIKKYDRVAILAHNTLHHVLTIFGCAKAGAIYLGLNYLMYGKDLAYCINHSEAKVLIV 136 Query: 119 GPDFVETAHAAAAKGSAVETLIWLPGEDPSTAPDGITTFDSLLHSDA-SAPDVSVDSRDP 177 E A + V+T +W +FD ++ + PD ++ DP Sbjct: 137 EDALFERIEPAMDQLKTVDTFLWSNQGVGVEGEGRWESFDDFFDGESDTEPDTILNIEDP 196 Query: 178 AQIIYTSGTESLPKGAILTHEAVMWQYVSCIIDGGMATDDTVLHALPLYHCAQLDVFLGP 237 Q+ YTSGTESLPK + T+E+++ QYV CI+DG +D +L+ +P+YHCAQ DVF+ P Sbjct: 197 VQMTYTSGTESLPKCVVHTNESLIAQYVGCIVDGEYTANDIMLNTMPIYHCAQRDVFMMP 256 Query: 238 AIYLGATSLITGKPVPDNVLALIAAHKINSFFAPPTIWIAMLRSGAFDRTDLSTLRKGYY 297 Y+G T+++ +P D +L I +K F PT+WI +LR D+ DL +LRK YY Sbjct: 257 IFYVGGTNVMMTEPNLDLMLEYIDRYKPTMLFLAPTVWIGLLRHPDIDKYDLQSLRKCYY 316 Query: 298 GASIMPVEVLLELQRRLPDVKFWNFYGQTEIAPLATVLSPEDQLPKAGSAGKHAINVETR 357 GASI P E L EL +LP +F+ +YGQTE+AP TV + E+ L +AG+ +N+E+R Sbjct: 317 GASIFPEEPLKELMEKLPQARFFQYYGQTELAPYHTVANHEEILEYTKTAGRSGLNLESR 376 Query: 358 VVRPDMTDV-EIGEVGEIVHRSPHLLSGYYNDPVKTAAAFAGGWFHSGDLATVDADGYIT 416 + + ++ EIG GEI R PH++ YY DP KT AF GWFHSGD+A + Y Sbjct: 377 LENDEHEEIAEIGVPGEITARGPHVMLMYYKDPEKTEEAFKCGWFHSGDIAVKYRESYFE 436 Query: 417 VVDRVKDMIKSGGENVASREVEEMIYRLPSVSEVAVVGLPDPRWIEAVTAIVVVKAGASL 476 +VDR KDMIK+GGENV+SREVEE+IY+ P V EVAV+GLP +WIEAVTAIVV K G ++ Sbjct: 437 IVDRKKDMIKTGGENVSSREVEEVIYKHPDVWEVAVIGLPHEKWIEAVTAIVVPKPGKTI 496 Query: 477 DAEAVIKHCAASMAHFKVPKQVIFV--DSLPKNPSGKLLKRELRQ 519 + E +I+ C ++ +KVPK VI + + LPK PSGK++KRELR+ Sbjct: 497 NPEEIIEFCRKELSPYKVPKGVIVLKPEDLPKTPSGKIMKRELRK 541 >Rv0166 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 554 Score = 276 bits (705), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 186/515 (36%), Positives = 271/515 (52%), Gaps = 13/515 (2%) Query: 12 AREHSIGDLLRRSAKRYPGKTALICGEVSWSFAEMDAICNRLGRGLLTLGVAKGDRVAVL 71 AR + + L R A P AL + ++A++ L L GV GDRV +L Sbjct: 23 ARRQNWVNQLERHAMMQPDAPALRFVGNTMTWADLRRRVAALAGALSGRGVGFGDRVMIL 82 Query: 72 SRNSHGFAALRFAVARIGAILVPINFMLNPDEINFILANSGAKLLAVGPDFVETAHAAAA 131 N F A IGAI VP+NF L P EI ++ + A ++ E A A A Sbjct: 83 MLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVM-----LTEAALAPVA 137 Query: 132 KG-SAVETLIWLPGEDPSTAPDGITTFDSLLHS--DASAPDVSVDSRDPAQIIYTSGTES 188 G ++ L+ + ++ D + ++ LL+ D P V + + PA I+YTSGT Sbjct: 138 IGVRNIQPLLSVIVVAGGSSQDSVFGYEDLLNEAGDVHEP-VDIPNDSPALIMYTSGTTG 196 Query: 189 LPKGAILTHEAVMWQYVSCIIDGGMATD-DTVLHALPLYHCAQLDVFLGPAIYLGATSLI 247 PKGA+LTH + Q ++ + G + D +PL+H A + L + T + Sbjct: 197 RPKGAVLTHANLTGQAMTALYTSGANINSDVGFVGVPLFHIAGIGNMLTGLLLGLPTVIY 256 Query: 248 -TGKPVPDNVLALIAAHKINSFFAPPTIWIAMLRSGAFDRTDLSTLRKGYYGASIMPVEV 306 G P +L ++ A K+ F P W A+ DL LR +GA+ P + Sbjct: 257 PLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCTEQQARPRDLR-LRVLSWGAAPAPDAL 315 Query: 307 LLELQRRLPDVKFWNFYGQTEIAPLATVLSPEDQLPKAGSAGKHAINVETRVVRPDMTDV 366 L ++ P+ + +GQTE++P+ +L ED + K GS G+ V RVV +M DV Sbjct: 316 LRQMSATFPETQILAAFGQTEMSPVTCMLLGEDAIAKRGSVGRVIPTVAARVVDQNMNDV 375 Query: 367 EIGEVGEIVHRSPHLLSGYYNDPVKTAAAFAGGWFHSGDLATVDADGYITVVDRVKDMIK 426 +GEVGEIV+R+P L+S Y+N+P TA AFAGGWFHSGDL +D+DGY+ VVDR KDMI Sbjct: 376 PVGEVGEIVYRAPTLMSCYWNNPEATAEAFAGGWFHSGDLVRMDSDGYVWVVDRKKDMII 435 Query: 427 SGGENVASREVEEMIYRLPSVSEVAVVGLPDPRWIEAVTAIVVVKAGASLDAEAVIKHCA 486 SGGEN+ E+E ++ P ++EVAV+G D +W E A+ V L E + + Sbjct: 436 SGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAV-TNDDLRIEDLGEFLT 494 Query: 487 ASMAHFKVPKQVIFVDSLPKNPSGKLLKRELRQRY 521 +A +K PK + VD+LP+NP+GK+LK ELR RY Sbjct: 495 DRLARYKHPKALEIVDALPRNPAGKVLKTELRLRY 529 >MT0175 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II MtC Mycobacterium tuberculosis CDC1551 Length = 518 Score = 271 bits (694), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 175/466 (37%), Positives = 253/466 (54%), Gaps = 13/466 (2%) Query: 61 GVAKGDRVAVLSRNSHGFAALRFAVARIGAILVPINFMLNPDEINFILANSGAKLLAVGP 120 GV GDRV +L N F A IGAI VP+NF L P EI ++ + A ++ Sbjct: 36 GVGFGDRVMILMLNRTEFVESVLAANMIGAIAVPLNFRLTPTEIAVLVEDCVAHVM---- 91 Query: 121 DFVETAHAAAAKG-SAVETLIWLPGEDPSTAPDGITTFDSLLHS--DASAPDVSVDSRDP 177 E A A A G ++ L+ + ++ D + ++ LL+ D P V + + P Sbjct: 92 -LTEAALAPVAIGVRNIQPLLSVIVVAGGSSQDSVFGYEDLLNEAGDVHEP-VDIPNDSP 149 Query: 178 AQIIYTSGTESLPKGAILTHEAVMWQYVSCIIDGGMATD-DTVLHALPLYHCAQLDVFLG 236 A I+YTSGT PKGA+LTH + Q ++ + G + D +PL+H A + L Sbjct: 150 ALIMYTSGTTGRPKGAVLTHANLTGQAMTALYTSGANINSDVGFVGVPLFHIAGIGNMLT 209 Query: 237 PAIYLGATSLI-TGKPVPDNVLALIAAHKINSFFAPPTIWIAMLRSGAFDRTDLSTLRKG 295 + T + G P +L ++ A K+ F P W A+ DL LR Sbjct: 210 GLLLGLPTVIYPLGAFDPGQLLDVLEAEKVTGIFLVPAQWQAVCTEQQARPRDLR-LRVL 268 Query: 296 YYGASIMPVEVLLELQRRLPDVKFWNFYGQTEIAPLATVLSPEDQLPKAGSAGKHAINVE 355 +GA+ P +L ++ P+ + +GQTE++P+ +L ED + K GS G+ V Sbjct: 269 SWGAAPAPDALLRQMSATFPETQILAAFGQTEMSPVTCMLLGEDAIAKRGSVGRVIPTVA 328 Query: 356 TRVVRPDMTDVEIGEVGEIVHRSPHLLSGYYNDPVKTAAAFAGGWFHSGDLATVDADGYI 415 RVV +M DV +GEVGEIV+R+P L+S Y+N+P TA AFAGGWFHSGDL +D+DGY+ Sbjct: 329 ARVVDQNMNDVPVGEVGEIVYRAPTLMSCYWNNPEATAEAFAGGWFHSGDLVRMDSDGYV 388 Query: 416 TVVDRVKDMIKSGGENVASREVEEMIYRLPSVSEVAVVGLPDPRWIEAVTAIVVVKAGAS 475 VVDR KDMI SGGEN+ E+E ++ P ++EVAV+G D +W E A+ V Sbjct: 389 WVVDRKKDMIISGGENIYCAELENVLASHPDIAEVAVIGRADEKWGEVPIAVAAV-TNDD 447 Query: 476 LDAEAVIKHCAASMAHFKVPKQVIFVDSLPKNPSGKLLKRELRQRY 521 L E + + +A +K PK + VD+LP+NP+GK+LK ELR RY Sbjct: 448 LRIEDLGEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRLRY 493 >BS_yhfL [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Bsu Bacillus subtilis Length = 513 Score = 270 bits (690), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 173/508 (34%), Positives = 261/508 (51%), Gaps = 16/508 (3%) Query: 21 LRRSAKRYPGKTALICGEVSWSFAEMDAICNRLGRGLLTLGVAKGDRVAVLSRNSHGFAA 80 L +A P A + ++ E++ R GL G+ KGD +A+L NS F Sbjct: 7 LEETASEKPDSIACRFKDHMMTYQELNEYIQRFADGLQEAGMEKGDHLALLLGNSPDFII 66 Query: 81 LRFAVARIGAILVPINFMLNPDEINFILANSGAKLLAVGPD----FVETAHAAAAKGSAV 136 F + G ++VPIN + P EI ++L N K + VG E+ H + K V Sbjct: 67 AFFGALKAGIVVVPINPLYTPTEIGYMLTNGDVKAI-VGVSQLLPLYESMHESLPKVELV 125 Query: 137 ETLIWLPGEDPSTAPD-----GITTFDSLLH-SDASAPDVSVDSRDPAQIIYTSGTESLP 190 ++ GE A D +TTF +L + A+ + D A I+YTSGT P Sbjct: 126 --ILCQTGEAEPEAADPEVRMKMTTFAKILRPTSAAKQNQEPVPDDTAVILYTSGTTGKP 183 Query: 191 KGAILTHEAVMWQYVSCIIDGGMATDDTVLHALPLYHCAQLDVFLGPAIYLGATSLITGK 250 KGA+LTH+ + GM D V+ ALP+ H L V + + GAT LI + Sbjct: 184 KGAMLTHQNLYSNANDVAGYLGMDERDNVVCALPMCHVFCLTVCMNAPLMSGATVLIEPQ 243 Query: 251 PVPDNVLALIAAHKINSFFAPPTIWIAMLRSGAFDRTDLSTLRKGYYGASIMPVEVLLEL 310 P +V L+ + F PT++ + + + D S++R G + MPV +L Sbjct: 244 FSPASVFKLVKQQQATIFAGVPTMYNYLFQHENGKKDDFSSIRLCISGGASMPVALLTAF 303 Query: 311 QRRLPDVKFWNFYGQTEIAPLATVLSPEDQLPKAGSAGKHAINVETRVVRPDMTDVEIGE 370 + + V YG +E +P+ T +P D+ K GS G ++VE +VV P ++ + Sbjct: 304 EEKF-GVTILEGYGLSEASPV-TCFNPFDRGRKPGSIGTSILHVENKVVDPLGRELPAHQ 361 Query: 371 VGEIVHRSPHLLSGYYNDPVKTAAAFAGGWFHSGDLATVDADGYITVVDRVKDMIKSGGE 430 VGE++ + P+++ GYY P++T A GW ++GDLA D DGY +VDR KDMI GG Sbjct: 362 VGELIVKGPNVMKGYYKMPMETEHALKDGWLYTGDLARRDEDGYFYIVDRKKDMIIVGGY 421 Query: 431 NVASREVEEMIYRLPSVSEVAVVGLPDPRWIEAVTAIVVVKAGASLDAEAVIKHCAASMA 490 NV REVEE++Y P V E V+G+PDP+ EAV VV K + + E +++HC +A Sbjct: 422 NVYPREVEEVLYSHPDVKEAVVIGVPDPQSGEAVKGYVVPKR-SGVTEEDIMQHCEKHLA 480 Query: 491 HFKVPKQVIFVDSLPKNPSGKLLKRELR 518 +K P + F+D +PKN +GK+L+R LR Sbjct: 481 KYKRPAAITFLDDIPKNATGKMLRRALR 508 >Rv3089 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Mtu Mycobacterium tuberculosis H37Rv Length = 503 Score = 270 bits (689), Expect = 9e-72, Method: Compositional matrix adjust. Identities = 184/512 (35%), Positives = 271/512 (52%), Gaps = 21/512 (4%) Query: 15 HSIGDLLRRSAKRYPGKTALI--CGEVSWSFAEMDAICNRLGRGLLTLGVAKGDRVAVLS 72 +IG +LR+ A P A + +V ++A+M+A+ NR L LG+AKGDRVA+L Sbjct: 2 KNIGWMLRQRATVSPRLQAYVEPSTDVRMTYAQMNALANRCADVLTALGIAKGDRVALLM 61 Query: 73 RNSHGFAALRFAVARIGAILVPINFMLNPDEINFILANSGAKLLAVGPDFVETAHAAAAK 132 NS F L + A++GA+ VPIN L E++FIL++SG+K++ G A Sbjct: 62 PNSVEFCCLFYGAAKLGAVAVPINTRLAAPEVSFILSDSGSKVVIYG-----------AP 110 Query: 133 GSAVETLIWLPGEDPSTAPDGI---TTFDSLLHSDASAPDVSVDSRDPAQIIYTSGTESL 189 + V I + P T D I + + L + A P V D I+YTSGT Sbjct: 111 SAPVIDAIRAQADPPGTVTDWIGADSLAERLRSAAADEPAVECGGDDNLFIMYTSGTTGH 170 Query: 190 PKGAILTHEAVMWQYVSCIIDGGMATDDTVLHALPLYHCAQLDVFLGPAIYLGATSLITG 249 PKG + THE+V S + D +L LP++H A L + A+ G T + Sbjct: 171 PKGVVHTHESVHSAASSWASTIDVRYRDRLLLPLPMFHVAALTTVIFSAMR-GVTLISMP 229 Query: 250 KPVPDNVLALIAAHKINSFFAPPTIWIAMLRSGAFDRTDLSTLRKGYYGASIMPVEVLLE 309 + V +LI ++ A P I M + F D R G + MP E L++ Sbjct: 230 QFDATKVWSLIVEERVCIGGAVPAILNFMRQVPEFAELDAPDFRYFITGGAPMP-EALIK 288 Query: 310 LQRRLPDVKFWNFYGQTEIAPLATVLSPEDQLPKAGSAGKHAINVETRVVRPDMTDVEIG 369 + +++ Y TE T+L ED L KAGSAG+ + + V D E G Sbjct: 289 IYA-AKNIEVVQGYALTESCGGGTLLLSEDALRKAGSAGRATMFTDVAVRGDDGVIREHG 347 Query: 370 EVGEIVHRSPHLLSGYYNDPVKTAAAFAGGWFHSGDLATVDADGYITVVDRVKDMIKSGG 429 E GE+V +S LL Y+N P T AF GWF +GD+ +D +GY+ + DR+KDMI SGG Sbjct: 348 E-GEVVIKSDILLKEYWNRPEATRDAFDNGWFRTGDIGEIDDEGYLYIKDRLKDMIISGG 406 Query: 430 ENVASREVEEMIYRLPSVSEVAVVGLPDPRWIEAVTAIVVVKAGASLDAEAVIKHCAASM 489 ENV E+E +I +P VSEVAV+GLPD +W E AIVV + + ++++C + Sbjct: 407 ENVYPAEIESVIIGVPGVSEVAVIGLPDEKWGEIAAAIVVADQN-EVSEQQIVEYCGTRL 465 Query: 490 AHFKVPKQVIFVDSLPKNPSGKLLKRELRQRY 521 A +K+PK+VIF +++P+NP+GK+LK LR++Y Sbjct: 466 ARYKLPKKVIFAEAIPRNPTGKILKTVLREQY 497