BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0165.0002.p (357 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0996 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Rs... 505 e-143 yeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Eco E... 370 e-102 ZyeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase EcZ ... 370 e-102 ECs2509 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Ec... 370 e-102 SMa1846 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Sm... 352 7e-97 >RSp0996 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Rso Ralstonia solanacearum Length = 361 Score = 505 bits (1301), Expect = e-143, Method: Compositional matrix adjust. Identities = 243/353 (68%), Positives = 280/353 (79%), Gaps = 1/353 (0%) Query: 1 MRTHSIAAIPADGIGPEVISAGVRVLEALAKRSGDISFNVKTFDWGSNYYKKHGVMMPAD 60 M+T+ IA IP DGIG EV+ AG V+EALA F + FDWG +YY++HG MMPAD Sbjct: 1 MKTYRIATIPGDGIGKEVVPAGREVMEALAAAGAGFRFEFENFDWGGDYYRQHGAMMPAD 60 Query: 61 GLAELKNFDAIYFGAVGAPDVPDHITLWGLRLPICQGFDQYANVRPTKILPGVASPLRNV 120 GL L+ DAI FG+ G +PDHITLWGLRL ICQGFDQYANVRPT+ILPG+ +PL+ Sbjct: 61 GLDALRGKDAILFGSAGDAQIPDHITLWGLRLKICQGFDQYANVRPTRILPGIDAPLKRC 120 Query: 121 GVGDLDWVIVRENSEGEYAGMGGRAHRGLPEEVGTEVAVFTRVGVTRIMRYAFQLAQSRP 180 G DLDWVIVRENSEGEYAG+GGR H+G P E T+V++ TR GV RIMR+AF+LAQSRP Sbjct: 121 GPQDLDWVIVRENSEGEYAGVGGRVHQGHPIEAATDVSMMTRAGVERIMRFAFRLAQSRP 180 Query: 181 RKLLTVVTKSNAQRHGMVMWDEIAAEVAKDFPDVTWDKMLVDAMTVRMTLNPKSLDTIVA 240 RKLLTV+TKSNAQRH MVMWDEIA +V+K+FPDV WDK LVDA T RM P SLDTIVA Sbjct: 181 RKLLTVITKSNAQRHAMVMWDEIAVQVSKEFPDVQWDKELVDAATARMVNRPASLDTIVA 240 Query: 241 TNLHADILSDLAGALAGSLGVAPTGNIDPQRRYPSMFEPIHGSAFDITGKGIANPVATFW 300 TNLHADILSDLA ALAGSLG+APTGNIDP+RRYPSMFEPIHGSAFDI GKG+ANPV TFW Sbjct: 241 TNLHADILSDLAAALAGSLGIAPTGNIDPERRYPSMFEPIHGSAFDIMGKGLANPVGTFW 300 Query: 301 TGAQMLEHLGEKDAAARLMAAVEKVCAAGVL-TPDVGGKATTKEVTDAVIDAI 352 + +LEHLGE+ AA RLM A+E V A L T D+GG ATT +VT AV + + Sbjct: 301 SVVMLLEHLGEQAAARRLMQAIEAVTANPALHTRDLGGNATTVQVTRAVCECL 353 >yeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Eco Escherichia coli K12 Length = 361 Score = 370 bits (950), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/359 (51%), Positives = 241/359 (67%), Gaps = 8/359 (2%) Query: 1 MRTHSIAAIPADGIGPEVISAGVRVLEALAKRSGDISFNVKTFDWGS-NYYKKHGVMMPA 59 M+T IAAIP DGIG EV+ G+RVL+A A+R G + + + +W S YY HG MMP Sbjct: 2 MKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWG-FALSFEQMEWASCEYYSHHGKMMPD 60 Query: 60 DGLAELKNFDAIYFGAVGAPD-VPDHITLWGLRLPICQGFDQYANVRPTKILPGVASPLR 118 D +L FDAIYFGAVG PD VPDHI+LWG L + FDQY N+RP ++ PGV PL Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 119 NVGVGDLDWVIVRENSEGEYAGMGGRAHRGLPEEVGTEVAVFTRVGVTRIMRYAFQLAQS 178 GD+D+ +VREN+EGEY+ +GGR + G EV + +VFTR GV RI+RYAF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 179 RPRKLLTVVTKSNAQRHGMVMWDEIAAEVAKDFPDVTWDKMLVDAMTVRMTLNPKSLDTI 238 RPRK LT TKSN M WDE +A+++P++ WDK +D + R + P+ D + Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 239 VATNLHADILSDLAGALAGSLGVAPTGNIDPQRRYPSMFEPIHGSAFDITGKGIANPVAT 298 VA+NL DILSDL A G++G+AP+ N++P+R +PS+FEP+HGSA DI GK IANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 299 FWTGAQMLEHLGEKD-----AAARLMAAVEKVCAAGVLTPDVGGKATTKEVTDAVIDAI 352 W GA ML+ LG D A ++AA+E+V A G TPD+ G ATT +V DA+ I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKII 359 >ZyeaU [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase EcZ Escherichia coli O157:H7 EDL933 Length = 361 Score = 370 bits (949), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/359 (51%), Positives = 241/359 (67%), Gaps = 8/359 (2%) Query: 1 MRTHSIAAIPADGIGPEVISAGVRVLEALAKRSGDISFNVKTFDWGS-NYYKKHGVMMPA 59 M+T IAAIP DGIG EV+ G+RVL+A A+R G + + + +W S YY HG MMP Sbjct: 2 MKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWG-FALSFEQMEWASCEYYSHHGKMMPD 60 Query: 60 DGLAELKNFDAIYFGAVGAPD-VPDHITLWGLRLPICQGFDQYANVRPTKILPGVASPLR 118 D +L FDAIYFGAVG PD VPDHI+LWG L + FDQY N+RP ++ PGV PL Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 119 NVGVGDLDWVIVRENSEGEYAGMGGRAHRGLPEEVGTEVAVFTRVGVTRIMRYAFQLAQS 178 GD+D+ +VREN+EGEY+ +GGR + G EV + +VFTR GV RI+RYAF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 179 RPRKLLTVVTKSNAQRHGMVMWDEIAAEVAKDFPDVTWDKMLVDAMTVRMTLNPKSLDTI 238 RPRK LT TKSN M WDE +A+++P++ WDK +D + R + P+ D + Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 239 VATNLHADILSDLAGALAGSLGVAPTGNIDPQRRYPSMFEPIHGSAFDITGKGIANPVAT 298 VA+NL DILSDL A G++G+AP+ N++P+R +PS+FEP+HGSA DI GK IANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 299 FWTGAQMLEHLGEKD-----AAARLMAAVEKVCAAGVLTPDVGGKATTKEVTDAVIDAI 352 W GA ML+ LG D A ++AA+E+V A G TPD+ G ATT +V DA+ I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGSATTPQVADAICKII 359 >ECs2509 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Ecs Escherichia coli O157:H7 Length = 361 Score = 370 bits (949), Expect = e-102, Method: Compositional matrix adjust. Identities = 184/359 (51%), Positives = 241/359 (67%), Gaps = 8/359 (2%) Query: 1 MRTHSIAAIPADGIGPEVISAGVRVLEALAKRSGDISFNVKTFDWGS-NYYKKHGVMMPA 59 M+T IAAIP DGIG EV+ G+RVL+A A+R G + + + +W S YY HG MMP Sbjct: 2 MKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWG-FALSFEQMEWASCEYYSHHGKMMPD 60 Query: 60 DGLAELKNFDAIYFGAVGAPD-VPDHITLWGLRLPICQGFDQYANVRPTKILPGVASPLR 118 D +L FDAIYFGAVG PD VPDHI+LWG L + FDQY N+RP ++ PGV PL Sbjct: 61 DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120 Query: 119 NVGVGDLDWVIVRENSEGEYAGMGGRAHRGLPEEVGTEVAVFTRVGVTRIMRYAFQLAQS 178 GD+D+ +VREN+EGEY+ +GGR + G EV + +VFTR GV RI+RYAF+LAQS Sbjct: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180 Query: 179 RPRKLLTVVTKSNAQRHGMVMWDEIAAEVAKDFPDVTWDKMLVDAMTVRMTLNPKSLDTI 238 RPRK LT TKSN M WDE +A+++P++ WDK +D + R + P+ D + Sbjct: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240 Query: 239 VATNLHADILSDLAGALAGSLGVAPTGNIDPQRRYPSMFEPIHGSAFDITGKGIANPVAT 298 VA+NL DILSDL A G++G+AP+ N++P+R +PS+FEP+HGSA DI GK IANP+AT Sbjct: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300 Query: 299 FWTGAQMLEHLGEKD-----AAARLMAAVEKVCAAGVLTPDVGGKATTKEVTDAVIDAI 352 W GA ML+ LG D A ++AA+E+V A G TPD+ G ATT +V DA+ I Sbjct: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGSATTPQVADAICKII 359 >SMa1846 [CE] COG0473 Isocitrate/isopropylmalate dehydrogenase Sme Sinorhizobium meliloti Length = 347 Score = 352 bits (904), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 179/353 (50%), Positives = 241/353 (68%), Gaps = 8/353 (2%) Query: 1 MRTHSIAAIPADGIGPEVISAGVRVLEALAKRSGDISFNVKTFDWGSNYYKKHGVMMPAD 60 M+++SIA IP DGIG +V A +VL +A+ SG + +F W +YK+ G MMPAD Sbjct: 1 MKSYSIALIPGDGIGQDVTDAAWQVLSTVARHSG-FTLTGTSFPWSCAFYKETGAMMPAD 59 Query: 61 GLAELKNFDAIYFGAVGAP-DVPDHITLWGLRLPICQGFDQYANVRPTKILPGVASPLRN 119 G+ L+ FDAI G+VG P +VPD I+L GL LPI + F QYAN+RP ++L GV PL+ Sbjct: 60 GIEALRPFDAILLGSVGWPAEVPDSISLHGLLLPIRKAFVQYANIRPHRLLSGVEGPLK- 118 Query: 120 VGVGDLDWVIVRENSEGEYAGMGGRAHRGLPEEVGTEVAVFTRVGVTRIMRYAFQLAQSR 179 D D + VREN+EGEY+G GGR H+G +EV E ++FTR GV RI+R+AF+ A+ R Sbjct: 119 --ASDFDILCVRENTEGEYSGAGGRVHQGTADEVAVETSIFTRAGVERILRFAFEQARQR 176 Query: 180 PRKLLTVVTKSNAQRHGMVMWDEIAAEVAKDFPDVTWDKMLVDAMTVRMTLNPKSLDTIV 239 KL +V TKSNAQ+H MV WDE+ ++A ++PDV +DAM R+ + P+SLD +V Sbjct: 177 RGKLASV-TKSNAQKHSMVFWDEVTRQLADEYPDVEVTSYHIDAMAARVVMAPESLDVVV 235 Query: 240 ATNLHADILSDLAGALAGSLGVAPTGNIDPQRRYPSMFEPIHGSAFDITGKGIANPVATF 299 A+NL DIL+DL A+ G LG A + NI+P R PSMFEP+HGSA DI GIANP+A Sbjct: 236 ASNLFGDILTDLGAAIQGGLGFAASANINPDRSAPSMFEPVHGSAPDIAHLGIANPIAAI 295 Query: 300 WTGAQMLEHLGEKDAAARLMAAVEKVCAAGVLTPDVGGKATTKEVTDAVIDAI 352 W+GA MLEHLGE++AA +MAA+E+ G+ T V GK T+ +T AV+ A+ Sbjct: 296 WSGAMMLEHLGEREAAGMIMAALERTTIRGIGT--VPGKDRTRSITAAVLAAL 346