BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0163.0009.p (244 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG1503C [R] COG1234 Metal-dependent hydrolases of the beta-lact... 91 2e-18 MA2375 [R] COG1234 Metal-dependent hydrolases of the beta-lactam... 74 2e-13 lin0930 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 66 5e-11 CAC1584 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 66 5e-11 L125485 [R] COG1234 Metal-dependent hydrolases of the beta-lacta... 63 6e-10 >VNG1503C [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Hbs Halobacterium sp. NRC-1 Length = 247 Score = 90.9 bits (224), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 67/223 (30%), Positives = 107/223 (47%), Gaps = 14/223 (6%) Query: 1 MQLRFVGCGDAFGSGGRFNTCFHVCGARSNFLIDCGASSLPTMKRLEIDRDAIDLILITH 60 MQL F+G G A +G R T V S L+DCG+ L T+ R ++ + +D +L++H Sbjct: 1 MQLTFLGTGSAMPTGQRMQTGLLVEDGDSRLLVDCGSGVLHTLARTDVGYEGVDTVLLSH 60 Query: 61 FHGDHFAGLPFFLLDAQFSRRTRPLVIAGPQGIETKLPAVMEVMFEHSSKTKQRFDLSVV 120 H DH + L LL A++ L + GP G + + ++ V + R D+++ Sbjct: 61 HHLDHVSDL-LALLKARWLAGEETLTVVGPAGTDALVTDLLGV----HDYLQGRVDVTL- 114 Query: 121 TLAPRETRSFGEVTVTPYPV--VHGESGGPFLAYRVGAEGRIISYSADTEWTDALIPAAQ 178 RE G V V + V + E P LAYR G + + +SADTE D + A Sbjct: 115 ----REV-DVGSVGVAGFDVTAIETEHSMPGLAYRFGGDDGDLVFSADTEAFDGMGAFAD 169 Query: 179 DADLFIAE-AYMYEKTVKNHLSLKTLEAHLPAINAKRVVLTHM 220 A + + + ++ E V NH + L A L ++ + LTH+ Sbjct: 170 GASVLVHDCSFPDEVDVSNHPTPTQLGASLAGVDVDDLYLTHL 212 >MA2375 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Mac Methanosarcina acetivorans str.C2A Length = 251 Score = 74.3 bits (181), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 66/238 (27%), Positives = 107/238 (44%), Gaps = 13/238 (5%) Query: 1 MQLRFVGCGDAFGSGGRFNTCFHVCGARSNFLIDCGASSLPTMKRLEIDRDAIDLILITH 60 M+++F+G G A GR + V LIDCG+ L + +D +L++H Sbjct: 1 MEIKFLGTGVAIPQKGRVQSGVLVRLEEKPLLIDCGSGVLSRFPDAGVSHTEVDTVLLSH 60 Query: 61 FHGDHFAGLPFFLLDAQFSRRTRPLVIAGPQGIETKLPAVMEVMFEHSSKTKQRFDLSVV 120 H DH A L L+ A + R + + GP+G + V+ +E+ + D+ ++ Sbjct: 61 LHLDHVADL-VPLIKANWLRGKTDMRVYGPKGTDDWFSQVIGA-YEY---FQDAVDVDII 115 Query: 121 TLAP-RETRSFG-EVTVTPYPVVHGESGGPFLAYRVGAEGRIISYSADTEWTDALIPAAQ 178 L+P +E G + +T +H P L YR+ AE I YS DTE ++ A Sbjct: 116 ELSPGKEFTPEGFDCEITCASALHSV---PTLGYRISAEAGEIVYSGDTEPCRDIMDLAV 172 Query: 179 DADLFIAE-AYMYEKTVKNHLSLKTLEAHLPAINAK--RVVLTHMSDDVLGRIDDLPY 233 +ADL I E ++ V NH + TL N + + LTH ++ G + Y Sbjct: 173 EADLLIHECSFPAGIKVTNHTTPDTLAEMFGDYNGEIGSICLTHFYPEMRGHEKEAVY 230 >lin0930 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Lin Listeria innocua Length = 243 Score = 66.2 bits (160), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 65/243 (26%), Positives = 105/243 (43%), Gaps = 23/243 (9%) Query: 1 MQLRFVGCGDAFGSGGRFNTCFHVCGARSNFLIDCGASSLPTMKRLEIDRDAIDLILITH 60 M+L G + + + + A LID GAS++ M+ ID D ID +I+H Sbjct: 1 MKLTVFGHWGGYPVANEGTSSYLLEEAGFKLLIDVGASAVSIMQNF-IDPDDIDAAIISH 59 Query: 61 FHGDHFAG---LPFFLLDAQFSRRTRPLVIAGPQGIETKLPAVMEVMFEHSSKTKQRFDL 117 +H DH A L L +Q + L I G + E E ++ TK Sbjct: 60 YHPDHVADVGILQHIRLLSQKKEKPPVLPIYGHKEDERGYS-----YLEMANVTK----- 109 Query: 118 SVVTLAPRETRSFGEVTVTPYPVVHGESGGPFLAYRVGAEGRIISYSADTEWTDALIPAA 177 V +T G VT VH P A R+ A G+I Y+AD+ + D+ IP A Sbjct: 110 -AVEYKADDTLEIGPFKVTFLKTVHPV---PCYAMRIEAAGKIFVYTADSAYQDSFIPFA 165 Query: 178 QDADLFIAEAYMYEK---TVKNHLSLKTLEAHLPAINAKRVVLTHMSDDVLGRIDDLPYT 234 ++ADL + + + K H++ + N K ++L+H+ + +G ++ L Sbjct: 166 ENADLLVTDTNFFHDLAGKTKVHMASTEVAKIAKEANVKSLLLSHLPE--VGDLEVLKQE 223 Query: 235 AAS 237 AA+ Sbjct: 224 AAA 226 >CAC1584 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Cac Clostridium acetobutylicum Length = 313 Score = 66.2 bits (160), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 72/273 (26%), Positives = 100/273 (36%), Gaps = 53/273 (19%) Query: 1 MQLRFVGCGDAFGSGGRFNTCFHVCGARSNFLIDCGASSLPTMKRLEIDRDAIDLILITH 60 + + +GCG + + R T + LIDCG + +MK + ID+I TH Sbjct: 6 LDICLLGCGGSLPTSDRNLTSLLISYNGRKILIDCGEGTQVSMKEIAWGFKDIDVICFTH 65 Query: 61 FHGDHFAGLPFFLLDAQFSRRTRPLVIAGPQGIETKLPAVMEV---------MFEHSSKT 111 +H DH GL LL S R PL I GP+G+ + + V + E SK Sbjct: 66 YHADHVMGLTGLLLTIANSGRIDPLTIIGPEGLREVVKGLTVVAPFFPYEIELIELDSKC 125 Query: 112 KQRF-----------------DLSVVTLA----PRETRSF----GEVTVTPYPVVHGESG 146 F D S+ L+ R F + P + + Sbjct: 126 SDNFLDKVFKIEDVEIFALPVDHSIECLSYSVRVNRKRKFDVNKAKANEVPLKIWNELQR 185 Query: 147 GPFLAYR--------VGAEGR---IISYSADTEWTDALIPAAQDADLFIAEAYMY----- 190 G + Y V E R I+Y DT D+L A +DLF+ E MY Sbjct: 186 GKEITYENKLYVPDMVLGESRKGIKITYCTDTRPVDSLHKFAYKSDLFVCEG-MYGEEEK 244 Query: 191 -EKTV-KNHLSLKTLEAHLPAINAKRVVLTHMS 221 EK V K H+ A K + LTH S Sbjct: 245 KEKAVDKKHMIFSEAAGIAKAAEVKELWLTHFS 277 >L125485 [R] COG1234 Metal-dependent hydrolases of the beta-lactamase superfamily III Lla Lactococcus lactis Length = 250 Score = 63.2 bits (152), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 65/237 (27%), Positives = 113/237 (47%), Gaps = 48/237 (20%) Query: 30 NFLIDCGASSLPTMKRLEIDRDAIDLILITHFHGDHFAGLPFFLLDAQFSRRTRPLVIAG 89 N L+DCG+ ++ ++ E+ +D ++++H+H DH A L R+ R L G Sbjct: 31 NMLLDCGSRAVTELEH-ELQPTQLDAVILSHYHEDHIADLGAL-------RQYRQLYPLG 82 Query: 90 PQGIETKLPAVMEVMFEHSSKTKQRFDLSVVTLAPRETRSFGEVTVTPYPVVHGESGGPF 149 G + +M ++ H + ++ S +TL + S G + Y V +G GPF Sbjct: 83 QDGWD----GMMLPIYGHG---ENEYEFSKLTL---DKVSEGHL----YNVENGTKVGPF 128 Query: 150 -------------LAYRVGAE--GRIISYSADTEWTDALIPAAQDADLFIAEAYMYEKTV 194 A R+ G+++ Y+ DT + D L A++ADL +A+ Y ++ Sbjct: 129 DITFLKTVHPVVNYAMRIVERRTGQVLIYTGDTGYFDELTDFAKEADLLLADVYFFKDKA 188 Query: 195 K--NHLSLKTLEAHLPAINA--KRVVLTHMSDDVLGRIDDL---PYTAASDGMVVEL 244 K NHLS ++EA A +A K+++LTH+ G +D L AA D ++ +L Sbjct: 189 KMPNHLS--SIEAGQIAKDAKVKKLILTHLPQ--FGDLDILLTEAKEAAGDAVITDL 241