BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0161.0017.p (322 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMb20179 [S] COG3391 Uncharacterized conserved protein Sme Sinor... 382 e-106 MA1904_1 [S] COG3391 Uncharacterized conserved protein Mac Metha... 158 1e-38 MA3120 [S] COG3391 Uncharacterized conserved protein Mac Methano... 127 4e-29 MA0512 [S] COG3391 Uncharacterized conserved protein Mac Methano... 127 4e-29 MA1591 [S] COG3391 Uncharacterized conserved protein Mac Methano... 118 2e-26 >SMb20179 [S] COG3391 Uncharacterized conserved protein Sme Sinorhizobium meliloti Length = 324 Score = 382 bits (982), Expect = e-106, Method: Compositional matrix adjust. Identities = 186/322 (57%), Positives = 238/322 (73%), Gaps = 2/322 (0%) Query: 1 MWRRCLLTGMLVWLAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFS 60 M R L V L+A A A +VSNE+GN +TV+D+ SW V T G RPRGI S Sbjct: 1 MLRPALAIVSAVLLSAGSAEANKVFVSNERGNNITVLDSESWEVIATFPAGNRPRGITIS 60 Query: 61 RDGKFVMVAVGDDDTIQVIDTKTQEIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTV 120 DGK + V DDDT++V D +T + + TLPSGPDPELF D +G +Y+ANE+DN VTV Sbjct: 61 PDGKELYVCASDDDTVRVFDPETYKELHTLPSGPDPELFALDPSGNPLYIANEDDNLVTV 120 Query: 121 IDLEKRARIGDIQVGVEPEGMTISPDGKILINTSETTNMAHFIDTSTRQIVANVLVDSRP 180 +D++ R + ++ VGVEPEG+ +SPD K +INTSETTNMAHFID ST +IV NVLVD RP Sbjct: 121 VDVKTRQVLAEVPVGVEPEGVAVSPDAKTIINTSETTNMAHFIDASTYKIVHNVLVDQRP 180 Query: 181 RFAQFKRDGSELWVSSEIGGTVSIID--PVKREVTGKITFEIPGLRNEAIQPVGIGMTKD 238 R+A+F DG +L+VS+EIGGTVS+ID + ++T KITFE+PG+ E +QPVG+ TKD Sbjct: 181 RYAEFTADGKKLYVSAEIGGTVSVIDVSAAEPKITKKITFEVPGVLPEWLQPVGVKATKD 240 Query: 239 GKTAFIALGPANRVAVVDGVTHKVLKYLLVGQRVWHMDFTPDEKYLLVTNGVSNDVSVID 298 G F+ALGPANRVAV+DG T +VL Y LVGQRVW M FTP E++L+ TNG SNDVS+ID Sbjct: 241 GSRIFVALGPANRVAVIDGKTDEVLDYFLVGQRVWQMAFTPGEEFLITTNGNSNDVSIID 300 Query: 299 VAAQKVIKTIQVGELPWGITIA 320 V A+KVI+++QVGE PWG+ +A Sbjct: 301 VKAEKVIRSVQVGEQPWGVVVA 322 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 42/132 (31%), Positives = 67/132 (50%), Gaps = 10/132 (7%) Query: 25 YVSNEKGNTVTVIDTSSWTVTKTIKVG----------QRPRGIEFSRDGKFVMVAVGDDD 74 YVS E G TV+VID S+ T K+ +P G++ ++DG + VA+G + Sbjct: 193 YVSAEIGGTVSVIDVSAAEPKITKKITFEVPGVLPEWLQPVGVKATKDGSRIFVALGPAN 252 Query: 75 TIQVIDTKTQEIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEKRARIGDIQV 134 + VID KT E++D G + + N N N V++ID++ I +QV Sbjct: 253 RVAVIDGKTDEVLDYFLVGQRVWQMAFTPGEEFLITTNGNSNDVSIIDVKAEKVIRSVQV 312 Query: 135 GVEPEGMTISPD 146 G +P G+ ++PD Sbjct: 313 GEQPWGVVVAPD 324 >MA1904_1 [S] COG3391 Uncharacterized conserved protein Mac Methanosarcina acetivorans str.C2A Length = 361 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 105/311 (33%), Positives = 160/311 (51%), Gaps = 11/311 (3%) Query: 6 LLTGMLVWLAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFSRDGKF 65 + LV ++ AS F AY++NE+ N+++VID S+ V TI VG P G+ + DG Sbjct: 53 MALAFLVLVSIAGASPF-AYITNEESNSISVIDVSTNKVAATIPVGSNPVGVAINPDGTK 111 Query: 66 VMVAVGDDDTIQVIDTKTQEIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEK 125 V VA + + VIDT T + T+P+G P+ GKT+YVAN N+++VID Sbjct: 112 VYVANDHSNDVSVIDTATNAVTATVPAGSSPQGIAVSPDGKTIYVANLAGNSISVIDTTS 171 Query: 126 RARIGDIQVGVEPEGMTISPDGKILINTSETTNMAHFIDTSTRQIVANVLVDSRPRFAQF 185 + ++ G P G+ +SPDGK + T+ IDT+T+ +++ V V PR Sbjct: 172 NTVVSTVKTGRNPTGVAVSPDGKKVYVTNSEDKTVSIIDTATKVVISTVSVGKDPREIAV 231 Query: 186 KRDGSELWVSSEIGGTVSIIDPVKREVTGKITFEIPGLRNEAIQPVGIGMTKDGKTAFIA 245 DG++++V++ GTVS+ID VT + E P G+ +T DG ++ Sbjct: 232 TPDGAKVYVANSDSGTVSVIDVSTNSVTDTVKVE--------GAPFGVAVTPDGAKVYVT 283 Query: 246 LGPA--NRVAVVDGVTHKVLKYLLVGQRVWHMDFTPDEKYLLVTNGVSNDVSVIDVAAQK 303 + VAV+D T+KV + VG + TPD + V + N VSVID A Sbjct: 284 NYDQYFSTVAVIDVATNKVTATIPVGPDPVGVAVTPDGTKVYVAINLCNTVSVIDTATNT 343 Query: 304 VIKTIQVGELP 314 V T+ VG+ P Sbjct: 344 VTATMPVGKSP 354 Score = 100 bits (250), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 65/222 (29%), Positives = 107/222 (48%), Gaps = 8/222 (3%) Query: 98 LFTQDAAGKTMYVANENDNTVTVIDLEKRARIGDIQVGVEPEGMTISPDGKILINTSETT 157 L + A Y+ NE N+++VID+ I VG P G+ I+PDG + ++ + Sbjct: 60 LVSIAGASPFAYITNEESNSISVIDVSTNKVAATIPVGSNPVGVAINPDGTKVYVANDHS 119 Query: 158 NMAHFIDTSTRQIVANVLVDSRPRFAQFKRDGSELWVSSEIGGTVSIIDPVKREVTGKIT 217 N IDT+T + A V S P+ DG ++V++ G ++S+ID V + Sbjct: 120 NDVSVIDTATNAVTATVPAGSSPQGIAVSPDGKTIYVANLAGNSISVIDTTSNTVVSTVK 179 Query: 218 FEIPGLRNEAIQPVGIGMTKDGKTAFIALGPANRVAVVDGVTHKVLKYLLVGQRVWHMDF 277 RN P G+ ++ DGK ++ V+++D T V+ + VG+ + Sbjct: 180 TG----RN----PTGVAVSPDGKKVYVTNSEDKTVSIIDTATKVVISTVSVGKDPREIAV 231 Query: 278 TPDEKYLLVTNGVSNDVSVIDVAAQKVIKTIQVGELPWGITI 319 TPD + V N S VSVIDV+ V T++V P+G+ + Sbjct: 232 TPDGAKVYVANSDSGTVSVIDVSTNSVTDTVKVEGAPFGVAV 273 Score = 89.0 bits (219), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 61/201 (30%), Positives = 90/201 (44%), Gaps = 44/201 (21%) Query: 25 YVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFSRDGKFVMVAVGDDDTIQVIDTKTQ 84 YV+N GN+++VIDT+S TV T+K G+ P G+ S DGK V V +D T+ +IDT T+ Sbjct: 155 YVANLAGNSISVIDTTSNTVVSTVKTGRNPTGVAVSPDGKKVYVTNSEDKTVSIIDTATK 214 Query: 85 EIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEKR------------------ 126 ++ T+ G DP G +YVAN + TV+VID+ Sbjct: 215 VVISTVSVGKDPREIAVTPDGAKVYVANSDSGTVSVIDVSTNSVTDTVKVEGAPFGVAVT 274 Query: 127 ---ARI-----------------------GDIQVGVEPEGMTISPDGKILINTSETTNMA 160 A++ I VG +P G+ ++PDG + N Sbjct: 275 PDGAKVYVTNYDQYFSTVAVIDVATNKVTATIPVGPDPVGVAVTPDGTKVYVAINLCNTV 334 Query: 161 HFIDTSTRQIVANVLVDSRPR 181 IDT+T + A + V PR Sbjct: 335 SVIDTATNTVTATMPVGKSPR 355 Score = 60.8 bits (146), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 37/128 (28%), Positives = 65/128 (50%), Gaps = 8/128 (6%) Query: 193 WVSSEIGGTVSIIDPVKREVTGKITFEIPGLRNEAIQPVGIGMTKDGKTAFIALGPANRV 252 ++++E ++S+ID T K+ IP N PVG+ + DG ++A +N V Sbjct: 71 YITNEESNSISVID----VSTNKVAATIPVGSN----PVGVAINPDGTKVYVANDHSNDV 122 Query: 253 AVVDGVTHKVLKYLLVGQRVWHMDFTPDEKYLLVTNGVSNDVSVIDVAAQKVIKTIQVGE 312 +V+D T+ V + G + +PD K + V N N +SVID + V+ T++ G Sbjct: 123 SVIDTATNAVTATVPAGSSPQGIAVSPDGKTIYVANLAGNSISVIDTTSNTVVSTVKTGR 182 Query: 313 LPWGITIA 320 P G+ ++ Sbjct: 183 NPTGVAVS 190 >MA3120 [S] COG3391 Uncharacterized conserved protein Mac Methanosarcina acetivorans str.C2A Length = 487 Score = 127 bits (319), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 105/326 (32%), Positives = 157/326 (48%), Gaps = 26/326 (7%) Query: 3 RRCLLTGMLVWLAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQR-PRGIEFSR 61 R L G V +A PA + V + +TV VIDT++ V+ + +G P I F+ Sbjct: 89 RVPLGGGWPVGVAVNPAGTKIYVVDSPSDSTVYVIDTATNKVSAKVNLGSSYPARITFNP 148 Query: 62 DGKFVMVAVGDDDT-IQVIDTKTQEIVDTLPSGPDPE--LFTQDAAGKTMYVANENDNTV 118 G V V D T I VI+ T ++ + GP FT D GK +Y AN N+NT+ Sbjct: 149 AGTRVYVTKQHDYTDIFVINPATNTLMAPIIVGPSTYGIAFTPD--GKKIYAANCNNNTI 206 Query: 119 TVIDLEKRARIGDIQVGVEPEGMTISPDGKILINTSETTNMAHFIDTSTRQIVANVLVDS 178 VID +I VG P G+ ++PDGK + + +N I+T+T ++A V V + Sbjct: 207 YVIDAATNKVTANISVGDSPCGIAVTPDGKKVYVLNYDSNTVSVINTATDNVIATVPVGN 266 Query: 179 RPRFAQFKRDGSELWVSSEIGGTVSIIDPVKREVTGKITFEIPGLRNEAIQPVGIGMTK- 237 P +G++++V++ TVS+ID V I PVG K Sbjct: 267 SPNLVAVTSNGNKVYVANN--NTVSVIDTATNNVIATI-------------PVGTSSRKI 311 Query: 238 ----DGKTAFIALGPANRVAVVDGVTHKVLKYLLVGQRVWHMDFTPDEKYLLVTNGVSND 293 DG ++ +N V+V+D T V+ + VG++ + TPD K + VTN S++ Sbjct: 312 VINPDGNRVYLVNFGSNSVSVIDTATDNVIANVPVGEQPMGIAVTPDGKRVYVTNSRSSN 371 Query: 294 VSVIDVAAQKVIKTIQVGELPWGITI 319 VSVID A V T+ G LP G+ I Sbjct: 372 VSVIDTATDTVAATVNAGILPTGVVI 397 Score = 65.1 bits (157), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 44/132 (33%), Positives = 64/132 (48%), Gaps = 2/132 (1%) Query: 14 LAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFSRDGKFVMVAVGDD 73 L A ++ YV+N NTV+VIDT++ V TI VG R I + DG V + Sbjct: 270 LVAVTSNGNKVYVANN--NTVSVIDTATNNVIATIPVGTSSRKIVINPDGNRVYLVNFGS 327 Query: 74 DTIQVIDTKTQEIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEKRARIGDIQ 133 +++ VIDT T ++ +P G P GK +YV N + V+VID + Sbjct: 328 NSVSVIDTATDNVIANVPVGEQPMGIAVTPDGKRVYVTNSRSSNVSVIDTATDTVAATVN 387 Query: 134 VGVEPEGMTISP 145 G+ P G+ I P Sbjct: 388 AGILPTGVVIVP 399 >MA0512 [S] COG3391 Uncharacterized conserved protein Mac Methanosarcina acetivorans str.C2A Length = 461 Score = 127 bits (318), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 96/331 (29%), Positives = 160/331 (48%), Gaps = 28/331 (8%) Query: 9 GMLVWLAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFSRDGKFVMV 68 +L+ + A+ F AYV+N N ++VIDT++ VT T+ VG+ P G+ S DGK V V Sbjct: 54 AILILMGVAGATPF-AYVTNSDSNNISVIDTATNRVTATVGVGEHPAGVTVSPDGKKVYV 112 Query: 69 AVGDDDTIQVIDTKTQ-EIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEKRA 127 DDT+ +I+T T+ +++ T+ G P+ G YV + ++N V+VID Sbjct: 113 VNSYDDTVSIINTTTKNKVITTVKVGTYPQEIAVSPDGTKAYVTSFSNNIVSVIDTTTNK 172 Query: 128 RIGDIQVGVEPEGMTISPDGKILI------NTSETTNMAHFIDTSTRQIVANVLVDSRPR 181 ++VG P G+ +SPDGK + +++ T IDT+T + A + + Sbjct: 173 VTATVKVGNFPLGIAVSPDGKKVYVANSGGDSTNFTGTVSVIDTTTNTVAATIPTGNNSL 232 Query: 182 FAQFKRDGSELWVSSEIGGTVSIIDPVKREVTGKI------TFEIPGLRNEAIQPVGIGM 235 DG++ +V + + R+ T I T E+ + N P G+ + Sbjct: 233 RVAVSPDGTKAYVMNS---------NIYRDSTAAIYVIDISTNEVTDVVNIGDIPCGVAV 283 Query: 236 TKDGKTAFIAL-----GPANRVAVVDGVTHKVLKYLLVGQRVWHMDFTPDEKYLLVTNGV 290 + DGK ++++ V ++D T+KV + VG+ + TPD + V N Sbjct: 284 SPDGKKVYVSIQYLSNSLTGAVNIIDTATNKVKATVSVGKAPSGIIVTPDGTKVYVVNCA 343 Query: 291 SNDVSVIDVAAQKVIKTIQVGELPWGITIAQ 321 S+ VSVID A +V T++VG P + I Sbjct: 344 SHTVSVIDTATSRVTDTVKVGIYPARVAIGH 374 >MA1591 [S] COG3391 Uncharacterized conserved protein Mac Methanosarcina acetivorans str.C2A Length = 443 Score = 118 bits (296), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 95/316 (30%), Positives = 155/316 (49%), Gaps = 19/316 (6%) Query: 10 MLVWLAATPASAFVAYVSNEKGNTVTVIDTSSWTVTKTIKVGQRPRGIEFSRDGKFVMVA 69 M++ L +A AY++N N ++VIDT++ VT T+ VG+ P G+ S DG V Sbjct: 43 MILILMDVVGAAPFAYITNSDSNNLSVIDTATNKVTATVDVGEHPAGVTVSPDGTKAYVV 102 Query: 70 VGDDDTIQVIDTKTQEIVDTLPSGPDPELFTQDAAGKTMYVANENDNTVTVIDLEKRARI 129 DDT+ +IDT +++ T+ G P+ G YV + ++NTV+VI+ Sbjct: 103 NSYDDTVSIIDTTKNKVIATVKVGTYPQNIAVSPDGTKAYVTSFSNNTVSVINTTINKVT 162 Query: 130 GDIQVGVEPEGMTISPDGKILI------NTSETTNMAHFIDTSTRQIVANVLVDSRPRFA 183 + VG P G+ +SPDGK + ++ T IDT+ A + + Sbjct: 163 ATVPVGNFPFGVAVSPDGKKVYVANSGGYSTNFTGTVSVIDTTANTAAATIPTGNNSLGV 222 Query: 184 QFKRDGSELWVSSEIGGTVSIIDPVKREVTGKITFEIPGLRNEAIQPVGIGMTKDGKTAF 243 DG +++V++ G ++SIID + V + P G+ ++ DGK + Sbjct: 223 AISPDGKKVYVANMAGSSISIIDTISNAVVSTV--------KTGRSPKGVAVSPDGKKIY 274 Query: 244 IALGPANR----VAVVDGVTHKVLKYLL-VGQRVWHMDFTPDEKYLLVTNGVSNDVSVID 298 +A+ P N V ++D T+KV + VG+ ++ TPD + V N S+ VSVID Sbjct: 275 VAINPINSLTGAVDIIDTATNKVTATAVPVGKAPGGIEVTPDGAKVYVVNYASHTVSVID 334 Query: 299 VAAQKVIKTIQVGELP 314 A KV T++VG+ P Sbjct: 335 TATNKVTDTVKVGKYP 350 Score = 73.2 bits (178), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 57/218 (26%), Positives = 103/218 (47%), Gaps = 14/218 (6%) Query: 109 YVANENDNTVTVIDLEKRARIGDIQVGVEPEGMTISPDGKILINTSETTNMAHFIDTSTR 168 Y+ N + N ++VID + VG P G+T+SPDG + + IDT+ Sbjct: 58 YITNSDSNNLSVIDTATNKVTATVDVGEHPAGVTVSPDGTKAYVVNSYDDTVSIIDTTKN 117 Query: 169 QIVANVLVDSRPRFAQFKRDGSELWVSSEIGGTVSIIDPVKREVTGKITFEIPGLRNEAI 228 +++A V V + P+ DG++ +V+S TVS+I+ K+T +P + N Sbjct: 118 KVIATVKVGTYPQNIAVSPDGTKAYVTSFSNNTVSVIN----TTINKVTATVP-VGN--- 169 Query: 229 QPVGIGMTKDGKTAFIA------LGPANRVAVVDGVTHKVLKYLLVGQRVWHMDFTPDEK 282 P G+ ++ DGK ++A V+V+D + + G + +PD K Sbjct: 170 FPFGVAVSPDGKKVYVANSGGYSTNFTGTVSVIDTTANTAAATIPTGNNSLGVAISPDGK 229 Query: 283 YLLVTNGVSNDVSVIDVAAQKVIKTIQVGELPWGITIA 320 + V N + +S+ID + V+ T++ G P G+ ++ Sbjct: 230 KVYVANMAGSSISIIDTISNAVVSTVKTGRSPKGVAVS 267