BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0152.0064.p (365 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1799 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 399 e-111 PA3415 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 362 e-100 BH0215 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex... 201 2e-51 APE1671 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 194 3e-49 PAE2648 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase comple... 189 1e-47 >RSc1799 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Rso Ralstonia solanacearum Length = 372 Score = 399 bits (1025), Expect = e-111, Method: Compositional matrix adjust. Identities = 227/369 (61%), Positives = 262/369 (71%), Gaps = 7/369 (1%) Query: 1 MRQFTLPDLGEGLEEAEIVTWYVNEGDHVVTDQPLVSVETDKAVVEIPAPSSGRIARLFG 60 M F LPDLGEGL+EAEIV W+V GD V DQPLVSVET KA+VEIP+P +GRIARLFG Sbjct: 1 MIVFKLPDLGEGLQEAEIVQWHVQAGDTVEADQPLVSVETAKAIVEIPSPQAGRIARLFG 60 Query: 61 AKGDIVKVGAPLVEFAEDAGQDTG--TIVGELDTGEQ-----PPAPATASPPSGQGAQVF 113 GDIV +GAPLV F G + T+VG++ G+Q P A A + G + Sbjct: 61 QPGDIVHLGAPLVAFEGAGGGEADAGTVVGQMQVGQQVVREAPAVLAAAPGAAAGGIKAT 120 Query: 114 PAVRALARKLDVALDLVEATGPGGTITRADVERAAKRMSHSGPAEPLRGLRRAMAQRMTA 173 PAVRALARKLDV L + +GP G IT ADVER AK S GP E LRG+RRAMAQ M Sbjct: 121 PAVRALARKLDVDLSMATPSGPDGVITAADVERVAKVFSELGPPEVLRGVRRAMAQNMAR 180 Query: 174 AHAEIVPATVTDEADIDDWRQGEDVTIRLARAIAAACKAEPALNAWYHSGTGERRLIERI 233 A +E+ ATV D+ADI W G DVTIRL RA+ A C+AEP LNAW+ G R ++E+I Sbjct: 181 AQSEVAAATVIDDADIHAWPPGTDVTIRLIRALVAGCRAEPGLNAWFDGHAGRRHVLEKI 240 Query: 234 DLGIAVDTGGGLIVPVLRNVAERSVSDLRAGLDRLRADAIARAIPPEELRGATITLSNFG 293 DLGIAVD GL VPVLRNVA R +DLRAGLDR+RAD AR IPPEELRG TITLSNFG Sbjct: 241 DLGIAVDLPDGLFVPVLRNVAHRDAADLRAGLDRMRADIRARKIPPEELRGNTITLSNFG 300 Query: 294 MIGGRFANLIIVPPQTAIVGAGRISERVVALRGQVAVRRTLPLSLTFDHRVVTGGEAARF 353 MI G++A ++VPP AI+GAGRI E+VVA G AV R LPLSLTFDHRVVTGGEAARF Sbjct: 301 MIAGKYAAPVVVPPTVAILGAGRIHEQVVAAAGAPAVHRVLPLSLTFDHRVVTGGEAARF 360 Query: 354 LVVLKSDVE 362 L +D+E Sbjct: 361 LAATIADLE 369 >PA3415 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Pae Pseudomonas aeruginosa Length = 370 Score = 362 bits (929), Expect = e-100, Method: Compositional matrix adjust. Identities = 199/368 (54%), Positives = 250/368 (67%), Gaps = 6/368 (1%) Query: 1 MRQFTLPDLGEGLEEAEIVTWYVNEGDHVVTDQPLVSVETDKAVVEIPAPSSGRIARLFG 60 M+ F LPDLGEGL+EAEIV W+V GD V DQ LVSVET KA+V+IPAP G + +LFG Sbjct: 1 MKHFKLPDLGEGLQEAEIVEWHVKAGDSVRADQRLVSVETAKALVDIPAPYDGVVGKLFG 60 Query: 61 AKGDIVKVGAPLVEFAEDAGQDTGTIVGELDTG-----EQPPAPATASPPSGQGAQVFPA 115 A+GDI+ VG PLV F E D GT+VG L+ G ++ A S + PA Sbjct: 61 AEGDILHVGEPLVGF-EGEEADAGTVVGRLEGGGSALEDRFFIGAAPSTREHLAPRATPA 119 Query: 116 VRALARKLDVALDLVEATGPGGTITRADVERAAKRMSHSGPAEPLRGLRRAMAQRMTAAH 175 VR AR+ V L + +GP G ITRADVE AA+ E LRG+RR+MA M +H Sbjct: 120 VRQFARQQGVELAGLSGSGPDGLITRADVEAAAQGTRERFGGERLRGVRRSMALNMARSH 179 Query: 176 AEIVPATVTDEADIDDWRQGEDVTIRLARAIAAACKAEPALNAWYHSGTGERRLIERIDL 235 AE+VP T+ +AD+ W+ D IRLA+A+A AC+AEP LNAW+ + +L ER+DL Sbjct: 180 AEVVPVTIYGDADLHRWKTARDPLIRLAQALAEACRAEPTLNAWFDGASLSLKLHERLDL 239 Query: 236 GIAVDTGGGLIVPVLRNVAERSVSDLRAGLDRLRADAIARAIPPEELRGATITLSNFGMI 295 GIAVDT GL VPVLR+V RS DLRAG+ RLR D AR+IPP E+ GAT+TLSNFG + Sbjct: 240 GIAVDTPDGLFVPVLRDVGARSAEDLRAGMRRLREDVQARSIPPAEMLGATLTLSNFGTL 299 Query: 296 GGRFANLIIVPPQTAIVGAGRISERVVALRGQVAVRRTLPLSLTFDHRVVTGGEAARFLV 355 GR+AN ++VPPQ AI+GAG I + VVA RG++A+ LPLSL+FDHR TGGEAARFL Sbjct: 300 FGRYANPVVVPPQVAILGAGGIRDEVVAWRGEMAIHPILPLSLSFDHRAATGGEAARFLK 359 Query: 356 VLKSDVER 363 VL + +E+ Sbjct: 360 VLVNALEQ 367 >BH0215 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Bha Bacillus halodurans Length = 414 Score = 201 bits (511), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 133/405 (32%), Positives = 208/405 (51%), Gaps = 42/405 (10%) Query: 1 MRQFTLPDLGEGLEEAEIVTWYVNEGDHVVTDQPLVSVETDKAVVEIPAPSSGRIARLFG 60 M +F LPD+GEG+ E EI++W+V EGDHV D+P+V V+TDK E+ AP SG+I R++ Sbjct: 1 MVEFRLPDVGEGMHEGEIISWFVQEGDHVKQDEPVVEVQTDKMNAELTAPVSGKIKRVYY 60 Query: 61 AKGDIVKVGAPLVEFAEDAG---QDTGTIVGELDTGEQPPAPATASPPSGQGAQV----- 112 G++ +VG+ L E+ +T ++ EQ + S S Q A V Sbjct: 61 KVGEVAEVGSLLFTIDENLSTFKSETHERTKRENSTEQTRPISNISLTSQQKAPVRKGLA 120 Query: 113 FPAVRALARKLDVALDLVEATGPGGTITR-------------------ADVERAAKRMSH 153 P VR LAR++++ L+ V TGPGG + A+V+ + +R Sbjct: 121 TPYVRQLAREMNINLEDVVGTGPGGRVLEQDLQNDTNLQKVKTVPSGVANVQESIERTGS 180 Query: 154 SGPAE-PLRGLRRAMAQRMTAAHAEIVPATVTDEADIDDWRQ--------GEDVTIRLA- 203 S PL+G+R+A+A++M + A I T DE ++D ++ E I+L Sbjct: 181 SAEKRIPLKGIRKAIAEKMIKSVATIPHVTHVDEIEMDALKELREQLKHYSEQKGIKLTF 240 Query: 204 -----RAIAAACKAEPALNAWYHSGTGERRLIERIDLGIAVDTGGGLIVPVLRNVAERSV 258 +AI +A K NA T E L + +GIA DT GLIVPV++N ++S+ Sbjct: 241 LPFFIKAIVSALKEFEYFNASIDEETNEIVLKKDYHIGIATDTEKGLIVPVIQNADQKSL 300 Query: 259 SDLRAGLDRLRADAIARAIPPEELRGATITLSNFGMIGGRFANLIIVPPQTAIVGAGRIS 318 +L + +L A + +++ G+T T+SN G IGG A II P+ AI+ ++ Sbjct: 301 LELAGEITQLSTQARKGTLNVQQMTGSTFTISNVGPIGGLHATPIINYPEVAILALHKME 360 Query: 319 ERVVALRGQVAVRRTLPLSLTFDHRVVTGGEAARFLVVLKSDVER 363 R V + ++ + +SL+FDHR+V G A RF +K +E Sbjct: 361 PRNVVREWESVIKLMMNMSLSFDHRLVDGATAVRFTNRMKELIEN 405 >APE1671 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Ape Aeropyrum pernix Length = 412 Score = 194 bits (493), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 142/407 (34%), Positives = 204/407 (50%), Gaps = 60/407 (14%) Query: 3 QFTLPDLGEGLEEAEIVTWYVNEGDHVVTDQPLVSVETDKAVVEIPAPSSGRIARLFGAK 62 Q LPD+GEG+ E EIV W V EG V PLV V T KA VEIP+P +GR+ RL Sbjct: 6 QVKLPDIGEGIAEGEIVEWLVEEGAVVKQFSPLVRVLTAKATVEIPSPYTGRVVRLLAKP 65 Query: 63 GDIVKVGAPLVEFAEDAGQDTGTIVGELDTGEQPPAPATASPPSGQGAQVF--------- 113 GD+V+VG P I E++ GE P AP A PS Sbjct: 66 GDVVRVGDP-------------IIEIEVEEGEAPKAPEAAEKPSATVEPPKAEEAAAPPP 112 Query: 114 -----------PAVRALARKLDVALDLVEATGPGGTITRADVERAAKRMSHSGPAE---- 158 P VR LAR+L V L V TGP G IT DV RAA ++ + E Sbjct: 113 QAAPAILVRAPPRVRRLARQLGVDLARVRGTGPRGAITEDDVRRAAAMLATAPKPEAPPP 172 Query: 159 ---------PLRGLRRAMAQRMTAAHAEIVPATVTDEADIDDW--------RQGEDVTIR 201 P+RG++R+MAQ M+ + ++I A + +E D + R E+ +R Sbjct: 173 VAEEAEERIPVRGIKRSMAQSMSLSKSKIPHAYIAEEVDFTELSKLREALKRDAEEKGVR 232 Query: 202 LA------RAIAAACKAEPALNAWYHSGTGERRLIERIDLGIAVDTGGGLIVPVLRNVAE 255 L +A+A A + P +N+ + E + + +++G AVDT GL+VPV++NV + Sbjct: 233 LTYLPFVFKAVAKAIRKYPLVNSEFDEEKMEIVVKKAVNIGFAVDTPHGLVVPVVKNVEK 292 Query: 256 RSVSDLRAGLDRLRADAIARAIPPEELRGATITLSNFGMIGGRFANLIIVPPQTAIVGAG 315 + + + + L A A + EE+ GAT T++N G IG +I PP AI+G Sbjct: 293 KGLFAIAREIADLTAKAREMRLSLEEVSGATFTITNVGSIGSVIGFPVIYPPNVAILGVH 352 Query: 316 RISERVVALRGQVAVRRTLPLSLTFDHRVVTGGEAARFLVVLKSDVE 362 R+ ER V + G++ R+ +SL+FDHR + G A RFL+ +K +E Sbjct: 353 RLVERPVYVDGELKPRKIGFVSLSFDHRALEGAYATRFLMEVKRLLE 399 >PAE2648 [C] COG0508 Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes Pya Pyrobaculum aerophilum Length = 383 Score = 189 bits (479), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 126/382 (32%), Positives = 200/382 (52%), Gaps = 45/382 (11%) Query: 3 QFTLPDLGEGLEEAEIVTWYVNEGDHVVTDQPLVSVETDKAVVEIPAPSSGRIARLFGAK 62 +F PDLGEGL E E++ W+V EGD V PLV V T+KA V +PAP++GR+ ++ + Sbjct: 2 EFKFPDLGEGLVEGEVIKWHVKEGDFVKEGDPLVDVMTEKATVTLPAPTTGRVVKILVRE 61 Query: 63 GDIVKVGAPLVEFAEDAGQDTGTIVGELDTGEQPPA-PATASPPSGQGAQVFPAVRALAR 121 G++VKVG L ++ E P A P T +P + PA R LA+ Sbjct: 62 GEVVKVGQTLCV---------------IEPAEGPAAGPQTEAPARPREVAAMPAARRLAK 106 Query: 122 KLDVALDLVEATGPGGTITRADVERAAKR-------------MSHSGPAE--PLRGLRRA 166 +L + L V+ TGPGG IT DV+R A+ + + AE P+RG+RRA Sbjct: 107 ELGIDLSKVKGTGPGGVITVEDVKRYAEETAKATAPAPAPKAVEKAEEAEVVPVRGIRRA 166 Query: 167 MAQRMTAAHAEIVPATVTDEADIDDW--------RQGEDVTIRL------ARAIAAACKA 212 +A++M+ A I A +E D + + E IRL A+A+A A + Sbjct: 167 VAEKMSKAKRLIPHAYHLEEVDFTELIKLRERVKAEAEKRGIRLTLLPFIAKAVAMALRE 226 Query: 213 EPALNAWYHSGTGERRLIERIDLGIAVDTGGGLIVPVLRNVAERSVSDLRAGLDRLRADA 272 P LN+ Y + + ++LGI VDT GL+V V++N ++ + ++ ++ L A Sbjct: 227 YPMLNSEYDEEKNAIVVKKEVNLGIGVDTEQGLVVVVVKNADKKGLLEMAKEINELAQKA 286 Query: 273 IARAIPPEELRGATITLSNFGMIGGRFANLIIVPPQTAIVGAGRISERVVALRGQVAVRR 332 + +++RG+T T+SN G +GG I+ P+ I+ G+ ++ A+ ++ +R Sbjct: 287 REGKLELQDVRGSTFTISNIGAVGGLGGLSILNYPEAGILAVGQARKKPWAVGDRIEIRD 346 Query: 333 TLPLSLTFDHRVVTGGEAARFL 354 L+++FDHRVV G ARF+ Sbjct: 347 IALLAVSFDHRVVDGAYVARFM 368