BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0146.0002.p (363 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH0575 [V] COG0534 Na+-driven multidrug efflux pump Pho Pyrococc... 85 3e-16 PH0577 [V] COG0534 Na+-driven multidrug efflux pump Pho Pyrococc... 84 4e-16 PAB1439 [V] COG0534 Na+-driven multidrug efflux pump Pab Pyrococ... 82 2e-15 PAB0243 [V] COG0534 Na+-driven multidrug efflux pump Pab Pyrococ... 73 1e-12 VC2671 [V] COG0534 Na+-driven multidrug efflux pump Vch Vibrio c... 70 9e-12 >PH0575 [V] COG0534 Na+-driven multidrug efflux pump Pho Pyrococcus horikoshii Length = 472 Score = 85.1 bits (209), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 68/271 (25%), Positives = 119/271 (43%), Gaps = 7/271 (2%) Query: 23 PRLLELLGGRGNVLAQAIAYSQVFFGGAILPWLMNTMAGILRGTGNMKLPSTMILSSAVW 82 P L + ++ A YS + F G + +L T ++R G+ + P + L + Sbjct: 135 PYALSFMHVTSSIRPYAYKYSLIIFAGVPMSFLFMTFNVLMRAIGDTRTPVKISLITVFL 194 Query: 83 QIILGGTLGLGLGPVPQFGMRGVAAGSLIAYSISISVMGWYLFSGRARVRPKLKGLRVQW 142 IIL L G+ G A ++++ S+ + L SGRA ++ + L+ W Sbjct: 195 NIILDPIFIFIL----NLGVEGAALATVLSNSVGTVIGVRILTSGRAGLKISRENLKPDW 250 Query: 143 AMFIDILKVGAISCFSPLQSVLTISIFTHMLAQFGTAVLAGYGIGARLEFMLTSVAFAVG 202 + I+++G S + + T ++ FG A Y I RL +TS++ + Sbjct: 251 KFYSKIVRIGLPSSIGQSANSFGFVVLTRIIMGFGDVTYAAYVITTRLVNFITSISRGIS 310 Query: 203 IASVPMIGMAVGAERVARARRIAWTAGTVSFLAVGVVGSFIAVFPDLWVNLFTSDPHVRA 262 +A M+ +GAE RA+ IA ++FL G+ I +F +F +DP V A Sbjct: 311 MAMSTMVAQNIGAENYERAKVIAERTMVINFLIAGIAVLIIGIFRVPIFKVFLNDPMVIA 370 Query: 263 TSH---QYLSTAAPMYAFIGLAMSMYFSSQG 290 S +Y + P + I + ++ FSS G Sbjct: 371 ESKIVLEYFLISVPFFNGIFVVVTGAFSSSG 401 >PH0577 [V] COG0534 Na+-driven multidrug efflux pump Pho Pyrococcus horikoshii Length = 449 Score = 84.3 bits (207), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 77/283 (27%), Positives = 128/283 (45%), Gaps = 13/283 (4%) Query: 16 LGMLTFGPRLLELLGGRGNVLAQAIAYSQVFFGGAILPWLMNTMAGILRGTGNMKLPSTM 75 LG+L P L+ + +V A+ Y ++ FGG + + ILR G+ K P + Sbjct: 114 LGILI-SPLALKFIKVTPDVYPYALKYMKIVFGGIPIALTLYAFNFILRAVGDTKTPVKV 172 Query: 76 ILSSAVWQIILGGTLGLGLGPVPQFGMRGVAAGSLIAYSISISVMGWYLFSGRARVRPKL 135 + + + IIL GL +G+ G A ++I+ + G+ LF+GR ++ L Sbjct: 173 NILTILLNIILDPVFIFGL----NYGVLGAALATVISEGTGSVIGGYLLFTGRVSIKLSL 228 Query: 136 KGLRVQWAMFIDILKVGAISCFSPLQSVLTISIFTHMLAQFGTAVLAGYGIGARL-EFML 194 + L ++I K+G + S + + ++ +GT A Y I RL FM Sbjct: 229 EDLSPDIKLYIKTFKIGLPASISESTNSFGFVLLNRIIYTYGTVAFATYTITNRLTNFMF 288 Query: 195 TSVAFAVGIASV--PMIGMAVGAERVARARRIAWTAGTVSFLAVGVVGSFIAVFPDLWVN 252 AFA GI+ M+G VGAE+ RA+ IA A +FL + +F + Sbjct: 289 ---AFADGISQAMGTMVGQNVGAEKYERAKEIAIKAMLTNFLILIFGTMLFIIFREEIFR 345 Query: 253 LFTSDPHVRATSHQYLSTAAPMYAFIGL--AMSMYFSSQGAAK 293 F +DP + S + + A + F G+ A++ F+S G K Sbjct: 346 FFINDPGILKESEKVVKYFAASFPFFGIFSAVTNVFASAGHTK 388 >PAB1439 [V] COG0534 Na+-driven multidrug efflux pump Pab Pyrococcus abyssi Length = 468 Score = 82.0 bits (201), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 69/271 (25%), Positives = 119/271 (43%), Gaps = 7/271 (2%) Query: 23 PRLLELLGGRGNVLAQAIAYSQVFFGGAILPWLMNTMAGILRGTGNMKLPSTMILSSAVW 82 P L+ + ++ A Y+ V F G + +L ++R TG+ K P + + Sbjct: 122 PLALDFMNVTPSIRPYAYRYAIVIFAGVPVSFLFMVFNALMRATGDTKTPVKISALTVFL 181 Query: 83 QIILGGTLGLGLGPVPQFGMRGVAAGSLIAYSISISVMGWYLFSGRARVRPKLKGLRVQW 142 IIL + + G+ G A ++++ ++ + L SG+A ++ L+ L+ W Sbjct: 182 NIILDPIFIF----IFKLGVLGAALATVLSNAVGTIIGVKILMSGKAGLKITLEDLKPDW 237 Query: 143 AMFIDILKVGAISCFSPLQSVLTISIFTHMLAQFGTAVLAGYGIGARLEFMLTSVAFAVG 202 + I++VG S + + T ++ FG A Y I RL +TS++ + Sbjct: 238 KFYSKIVRVGLPSSIGQSANSFGFVVLTRIIMGFGDVTYAAYVITTRLVNFITSISRGIS 297 Query: 203 IASVPMIGMAVGAERVARARRIAWTAGTVSFLAVGVVGSFIAVFPDLWVNLFTSDPHVRA 262 +A M+ VGAE+ RA+ IA V+F G I VF +F DP V A Sbjct: 298 MAMGTMVAQNVGAEKYERAKVIAERTMVVNFAIAGFAVLVIGVFRVPIFKVFLDDPEVIA 357 Query: 263 TSH---QYLSTAAPMYAFIGLAMSMYFSSQG 290 S +Y + P + I + ++ FSS G Sbjct: 358 ESKIVLEYFLISVPFFNGIFVVVTGVFSSAG 388 >PAB0243 [V] COG0534 Na+-driven multidrug efflux pump Pab Pyrococcus abyssi Length = 463 Score = 72.8 bits (177), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 68/265 (25%), Positives = 118/265 (44%), Gaps = 3/265 (1%) Query: 2 HALLIGLTFGATFMLGMLTFGPRLLELLGGRGNVLAQAIAYSQVFFGGAILPWLMNTMAG 61 ++ I G ML L G +L+++G +G VL A AY +V F + ++ Sbjct: 87 QSMYIAFIIGIPVMLFGLFLGDEVLKIMGAKGEVLEIAYAYLRVLFLFYPIRFMGFAFFS 146 Query: 62 ILRGTGNMKLPSTMILSSAVWQIILGGTLGLGLGPVPQFGMRGVAAGSLIAYSISISVMG 121 LRG G+ K P + + V +L L G P+ G G A S I +I+ V G Sbjct: 147 ALRGAGDTKTPMKLNILMNVINAVLDYLLVFGKFGFPRLGPVGAAWASGIGITIAFLV-G 205 Query: 122 WYLFSGRARVRPKLKGLRVQWAMFIDILKVGAISCFSPLQSVLTISIFTHMLAQFGTAVL 181 YLF + + L +++ + IL++G + ++ ++A+FG L Sbjct: 206 MYLFITNKLILKPVLDLSLRFDIIEKILRIGTPTMLERGLFSFYNFLYVSIVARFGKIAL 265 Query: 182 AGYGIGARLEFMLTSVAFAVGIASVPMIGMAVGAERVARARRIAWTAGTVSFLAVGVVGS 241 + + IG R+E + AF IA+ ++G +GA++ A R A ++ + + + Sbjct: 266 SAHYIGLRIESIAYMPAFGFSIATSALVGQNLGAKKPEEAERTVKEALKMTTMFMSAMAF 325 Query: 242 FIAVFPDLWVNLFTS--DPHVRATS 264 + VFP F S DP+ +A Sbjct: 326 VLMVFPGYLTEPFLSHNDPNYQAVK 350 >VC2671 [V] COG0534 Na+-driven multidrug efflux pump Vch Vibrio cholerae Length = 460 Score = 69.7 bits (169), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 65/211 (30%), Positives = 106/211 (50%), Gaps = 13/211 (6%) Query: 16 LGMLTFGPRLLELLGGRGNVLAQAIAYSQVFFGGAILPWLMNTMAG--ILRGTGNMKLPS 73 LG+LT P L LLG ++ Y V++ +P L+ MAG +R TG+ K P+ Sbjct: 122 LGLLTIEPLFL-LLGASHELIPLIKEYMVVWY--LTIPLLVLPMAGNSAIRATGDTKTPA 178 Query: 74 TMILSSAVWQIILGGTLGLGLGPVPQFGMRGVAAGSLIAYSISISVMG-WYLFSGRARVR 132 +++ + + L L G GP P+ G++G A S A+S +++G YL R ++ Sbjct: 179 KIMMLAGLINGALDPLLIFGYGPFPELGIQGAAIAS--AFSWLGALIGSLYLLIKREKLL 236 Query: 133 --PKLKGLRVQWAMFIDILKVGAISCFSPLQSVLTISIFTHMLAQFGTAVLAGYGIGARL 190 P L LR W ILK+G + S + ++ ++ ML+ GTA +A YG R+ Sbjct: 237 AIPSLPHLRDDWQ---QILKIGTPAALSTAMNPISGALLMIMLSSHGTAAVAAYGATQRI 293 Query: 191 EFMLTSVAFAVGIASVPMIGMAVGAERVARA 221 E +L V ++ A P + +GA+ AR+ Sbjct: 294 ESILILVLMSLTSALTPFMAQNLGAQNPARS 324