BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0144.0035.p (1227 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA3490 [L] COG1112 Superfamily I DNA and RNA helicases and helic... 383 e-105 MTH487 [L] COG1112 Superfamily I DNA and RNA helicases and helic... 347 9e-95 TM0618 [L] COG1112 Superfamily I DNA and RNA helicases and helic... 268 7e-71 PA2728 [L] COG1112 Superfamily I DNA and RNA helicases and helic... 184 1e-45 MPN153 [L] COG1112 Superfamily I DNA and RNA helicases and helic... 160 1e-38 >MA3490 [L] COG1112 Superfamily I DNA and RNA helicases and helicase subunits Mac Methanosarcina acetivorans str.C2A Length = 1939 Score = 383 bits (984), Expect = e-105, Method: Compositional matrix adjust. Identities = 213/513 (41%), Positives = 308/513 (60%), Gaps = 26/513 (5%) Query: 561 EARRRQFAEKDRAKIRDDRIRIKAKLMTKKPSPGSNVGRRRTWTEMALIANEIGKERGFV 620 E + +F E DR + ++R RI + P S R +E ++ NE ++RG + Sbjct: 878 EGKISKFTELDRKVLLENRKRIVCSAYEETPKLTSGASRA---SEAGILLNEFNRKRGHM 934 Query: 621 PVRTLLAQAGMSVQALKPCFMMSPLSLAKFMKAGSLSFDMLVIDEASQMRPEDALGAMLR 680 P+RTL+ +AG VQ +KPCF+MSPLS+A+++ S FD+++ DEASQ+RPEDA+GA+LR Sbjct: 935 PIRTLMKKAGSLVQKIKPCFLMSPLSIAQYLDPRSTRFDVIIFDEASQVRPEDAVGALLR 994 Query: 681 SKQIVVVGDQKQLPPTDFFARSGDGGSDEEDLDDY----DDESILESCQKTFGLRRPLRW 736 KQ+VV+GD KQLPPTDFF D S E + DDY D ESIL C+++F ++ LRW Sbjct: 995 GKQVVVMGDSKQLPPTDFFDTVVD--SPESEPDDYTSAGDMESILNVCKRSFPVKT-LRW 1051 Query: 737 HYRSRCESLIRFSNERFYKRELITFPA--SKPSSFSIDLVRVAGTFQAR----CNPVEAS 790 HYRSR ESLI SN+ FY L +P+ K S + V + R N +EA Sbjct: 1052 HYRSRHESLIAISNQEFYDNRLYVYPSPMQKDESLGLKFVYLPDAAYDRGKSGVNRIEAR 1111 Query: 791 RVAEEAITFMRHHAALNESDVPSLGLVALNIQQRDLIQEEFNRLITDDVLVEQYREKVAN 850 V+ + + SLG+ NI+Q++ I +E L + V+ + Sbjct: 1112 AVSRAVFEHFQKYPG------KSLGVGTFNIKQQEAILDEIEALQKANPGVD--LNSGND 1163 Query: 851 RGEEFFIKNLENVQGDERDFVFISMTYGPEPGSTVLKQRFGPINRKQGHRRLNVLFSRAR 910 RGE FF+KNLE +QGDERD + +S+ +G + + L FGP+NR+ G RRLNVL +RAR Sbjct: 1164 RGEHFFVKNLETIQGDERDVILLSVGFGFD-NNGRLSLNFGPLNREGGERRLNVLITRAR 1222 Query: 911 TRIGLFTSFSSSDVRPTSDSAEGVHVLQKYLEYAETRGRMTTSPGGDGEPDSDFETEVAD 970 + +F++F+S D+ +++ G+ L+ +LE+AE+ G + G E DS FE VA+ Sbjct: 1223 EKCVVFSNFTSRDLPIDENTSFGLKALKVFLEFAES-GTLPVQSCGRQELDSPFEEAVAE 1281 Query: 971 RLRARGFTVDYQVGASGYKIDLGVRHPDFAERYLAGIECDGAAYHSSKSARDRDRLREEV 1030 L G V Q+G +GY++DL V P RY+ GIECDGA+YHSS ARDRDRLR+++ Sbjct: 1282 FLTENGCEVHSQIGCAGYRLDLAVPDPRHPGRYVLGIECDGASYHSSPVARDRDRLRQQI 1341 Query: 1031 LNDKGWQILRVWSTDWFDDPALQTDRLVAKIEA 1063 L GW+I RVWSTDW+ P ++L+ ++A Sbjct: 1342 LEGLGWKIYRVWSTDWYLHPKECREKLLETVKA 1374 >MTH487 [L] COG1112 Superfamily I DNA and RNA helicases and helicase subunits Mth Methanothermobacter thermautotrophicus Length = 1157 Score = 347 bits (890), Expect = 9e-95, Method: Compositional matrix adjust. Identities = 214/521 (41%), Positives = 306/521 (58%), Gaps = 42/521 (8%) Query: 545 ARRSAQPLQNNTGASLEARRRQFAEKDRAKIRDDRIRIKAKLMTKKPSPGSNVGRRRTWT 604 A R L G EA+ R+F E D I +R R+ +L + P+ G + Sbjct: 634 AFREIPTLYTFIGDLHEAKIREFRELDSRIIELNRKRLIHQLNSNMPTV---FGGAAPNS 690 Query: 605 EMALIANEIGKERGFVPVRTLLAQAGMSVQALKPCFMMSPLSLAKFM--KAGSLSFDMLV 662 E ++++ E ++RG +P+R LL AG +V+ +KPCFMMSPLS+A+++ ++ L FD+++ Sbjct: 691 EESILSGEFTRKRGHMPLRKLLKLAGGTVKRIKPCFMMSPLSIAQYLDPRSSQLQFDVVI 750 Query: 663 IDEASQMRPEDALGAMLRSKQIVVVGDQKQLPPTDFFARSGDGGSDEEDLDDYDD-ESIL 721 DEASQ++PEDALGA LR++ VV+GD QLPPT FF + D ED+ D ESIL Sbjct: 751 FDEASQVKPEDALGAFLRARTAVVMGDTNQLPPTSFFDQMISAEEDSEDVATAADIESIL 810 Query: 722 ESCQKTFGLRRPLRWHYRSRCESLIRFSNERFYKRELITFP--ASKPSSFSIDLVRVAGT 779 C+++F +R LRWHYRSR ESLI SN+ FY L+ +P A + + LV + T Sbjct: 811 HLCKRSFPVRM-LRWHYRSRHESLIAVSNQEFYDNRLLVYPSPAREDEELGLHLVYLPDT 869 Query: 780 FQAR----CNPVEASRVAEEAIT--FMRHHAALNESDVPSLGLVALNIQQRDLIQEEFNR 833 R NP+EA+ V + AI FMR+ ++ SLG+ ++ Q + I E Sbjct: 870 VYERGRTSSNPLEAAAVVD-AIEEHFMRYGSS------RSLGVGTFSVAQMNAILEALEA 922 Query: 834 LITDDVLVEQYREKVANRGEEFFIKNLENVQGDERDFVFISMTYG-PEPGSTVLKQRFGP 892 + ++ +E+ + + E FFIKNLE +QGDERD +FIS+ YG E G L FGP Sbjct: 923 RLRENPELERIINQETD--EPFFIKNLETIQGDERDVIFISVGYGFDERGRMSLN--FGP 978 Query: 893 INRKQGHRRLNVLFSRARTRIGLFTSFSSSDVRPTSDSAEGVHVLQKYLEYAETRGRMTT 952 +N++ G RRLNVL +RAR + +FT+F SSD+R + GV L+++L YAET Sbjct: 979 LNQEGGERRLNVLITRAREKCVVFTNFRSSDLRTGPGTPFGVRALKRFLRYAET------ 1032 Query: 953 SPGGDGEPDSD------FETEVADRLRARGFTVDYQVGASGYKIDLGVRHPDFAERYLAG 1006 G E DS FE V D L GF VD VG +G+++D V+ PD RY+AG Sbjct: 1033 ---GFLESDSTDTSQGLFEDSVYDFLVDAGFEVDRGVGCAGFRVDFAVKDPDEPGRYIAG 1089 Query: 1007 IECDGAAYHSSKSARDRDRLREEVLNDKGWQILRVWSTDWF 1047 I DG Y ++ ARDRDRLREE+L + GW+I+ +WS+DW+ Sbjct: 1090 ILTDGEMYSRAEVARDRDRLREEILRNLGWKIIHLWSSDWY 1130 >TM0618 [L] COG1112 Superfamily I DNA and RNA helicases and helicase subunits Tma Thermotoga maritima Length = 1289 Score = 268 bits (684), Expect = 7e-71, Method: Compositional matrix adjust. Identities = 199/619 (32%), Positives = 312/619 (50%), Gaps = 44/619 (7%) Query: 452 HLRQYADKIQALRSGFGFVDLDDAQPVSVAEKLRP---LLDRKQELPEFLGLRHERRGLE 508 L++Y + + L GFV Q V E +R L+ + +L E++ L+ LE Sbjct: 683 QLKKYMEDLNRLWPD-GFVGEKTLQTAPVDELIRKIDHLIANESKLHEWIQLQETLESLE 741 Query: 509 KLGLGDFLKRADAARIAPSRLPQLLESAISRRGATLARRSAQPLQNNTGASLEARRRQFA 568 K+ L F++ + + R ++ E + ++ + L++ + E Sbjct: 742 KMELKTFIRSVEKEHVKNIR--RIFEKSFYKQWIDNVCTLDKNLRDFSSERYEQDILTL- 798 Query: 569 EKDRAKIRDDRIRIKAKLMTKKPSPGSNVGRRRTWTEMALIANEIGKERGFVPVRTLLAQ 628 EK +R ++ L+ KK + + ++ ++ E K+R P+R L + Sbjct: 799 EKAEEALRKRWVQYVKSLLAKKFR--TILADTNHSQQIRILMRETQKKRRHKPIRKFLLE 856 Query: 629 AGMSVQALKPCFMMSPLSLAKFMKAGSL--SFDMLVIDEASQMRPEDALGAMLRSKQIVV 686 ++ +KPC +MSPLS++ F+ FD+++ DEASQ+R +A+ A++R KQI+V Sbjct: 857 ISDILRFVKPCILMSPLSVSSFLDLDKFINYFDIVIFDEASQLRTPEAISAVVRGKQIIV 916 Query: 687 VGDQKQLPPTDFFARSGDGGSDEEDLDDYDDESILESC---QKTFGLRRPLRWHYRSRCE 743 GD KQLPPT+FF + DE++ + +S L+ C + F + LRWHYRSR E Sbjct: 917 AGDPKQLPPTNFFKSYYELEDDEDEREP--LDSFLDECIALPRVFK-QGYLRWHYRSRDE 973 Query: 744 SLIRFSNERFY-KRELITFPASK--PSSFSIDLVRVA-GTFQ---ARCNPVEASRVAEEA 796 LI FSN FY + LITFP+ K S I LV V GT+ R N +EA +V + Sbjct: 974 RLIAFSNHYFYGENPLITFPSPKYRNSDQGIKLVYVENGTWDRAGKRVNTMEALKVVDIV 1033 Query: 797 ITFMRHHAALNESDVPSLGLVALNIQQRDLIQEEFNRLITD-----DVLVEQYREKVANR 851 I + H S+G+V +N Q DLI+ R + + DV+ ++ Sbjct: 1034 IEHFQKHPD------RSIGVVTMNTSQSDLIENLLQRRLMEYPHLMDVIFKE-------S 1080 Query: 852 GEEFFIKNLENVQGDERDFVFISMTYGPEPGSTVLKQRFGPINRKQGHRRLNVLFSRART 911 E FFIK+LENVQGDERD + IS+ Y P + FGP+N + G RRLNVL +RAR Sbjct: 1081 NEPFFIKSLENVQGDERDTIIISIGYARTPSGELF-YNFGPLNNEGGWRRLNVLITRARY 1139 Query: 912 RIGLFTSFSSSDVRPTSDSAEGVHVLQKYLEYAETRGRMTTSPGGDGEPDSDFETEVADR 971 +I L TS S D+ + +GV L+ YL+YAE ++ GE D T +A + Sbjct: 1140 QIILVTSLRSEDLSRANSENKGVAALRNYLKYAEQNCKLEFCRSY-GESDDIIPTSIARQ 1198 Query: 972 LRARGFTVDYQVGASGYKIDLGVRHPDFAERYLAGIECDGAAYHSSKSARDRDRLREEVL 1031 L GF D +G ++ +G+ P+ RY GI DG + + DR+ L+ +VL Sbjct: 1199 LNNIGFLTDTNIGMGLCQVSVGIEDPEDPGRYKIGIIHDGKNHAMIQDVIDREVLKFKVL 1258 Query: 1032 NDKGWQILRVWSTDWFDDP 1050 GW++ R+W+ +W+ DP Sbjct: 1259 ESLGWKLKRLWTIEWYRDP 1277 >PA2728 [L] COG1112 Superfamily I DNA and RNA helicases and helicase subunits Pae Pseudomonas aeruginosa Length = 886 Score = 184 bits (467), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 162/561 (28%), Positives = 252/561 (44%), Gaps = 70/561 (12%) Query: 577 DDRIRIKAKLMTKKPSPGSNVGRRRTWTEMALIANEIGKE-RGFVPVRTLLAQAGMSVQA 635 D ++R + + K + +G R+ W ++ + + + R F+ + A + + + Sbjct: 341 DVQMRALNRELLGKGIDAARLGSRKQWEDVTRLTGKRSRRLREFIEL-----GAELGLMS 395 Query: 636 LKPCFMMSPLSLAKFMKAGSLSFDMLVIDEASQMRPEDALGAMLRSKQIVVVGDQKQLPP 695 L+P ++M+P ++ + + FDM++ DEASQM E AL + R + VV GD+KQ+PP Sbjct: 396 LRPVWLMNPDLASRVLPLKAGLFDMVIYDEASQMPVEFALPTLYRGRVTVVSGDEKQMPP 455 Query: 696 TDFF--------ARSGDGGSDEEDLDDYDDESI--------LESCQKTFGLRR------P 733 T FF A DG + +ED D+ E+ ++ C L R Sbjct: 456 TAFFSSRVESDEAELFDGEAPDEDADEEQREAYEDTWNRREIKDCPDLLQLARNALPSTT 515 Query: 734 LRWHYRSRCESLIRFSNERFYKRELITFPASKPSSF-----SIDLVRVAGTFQARCNPVE 788 L+ HYRS LI FSN FY L + P P + ++L++V G +Q + N E Sbjct: 516 LQIHYRSAYRELIGFSNASFYGNRL-SVPVRHPQANILRIKPLELIQVNGLYQNQSNERE 574 Query: 789 ASRVAEEAITFMRHHAALNESDVPSLGLVALNIQQRDLIQEEFN-RLITDDVLVEQYRE- 846 A RV + R A PS+G+V N +Q DLI+E R D+ Y E Sbjct: 575 AERVVDYLAELWRQPYAAR----PSVGVVTFNRKQADLIEERLELRAEQDETFRAAYSEE 630 Query: 847 -KVANRGEEF--FIKNLENVQGDERDFVFISMTYGPEPGSTVLKQRFGPINRKQGHRRLN 903 + + GE+ F+KN+ENVQGDERD + S T+G G ++ FG + + G RRLN Sbjct: 631 RERSEDGEDMGVFVKNVENVQGDERDVIVFSSTFG-RNGQGSFRRNFGVLGQTGGERRLN 689 Query: 904 VLFSRARTRIGLFTSFSSSDVRPTSDSAEGVH----VLQKYLEYAETRGRMTTSPGGDGE 959 V +RAR ++ + TS D+ + LQ YLEYA R G G+ Sbjct: 690 VAVTRARRKVVMITSMPIGDISDMLGTQRSPSSPRDYLQGYLEYA--RALSAGEFAGTGQ 747 Query: 960 PDSDFETE------------------VADRLRARGFTVDYQVGASGYKIDLGVRHPDFAE 1001 +T+ V + +R+ G++ + +D + +P Sbjct: 748 LLERLQTDRSDQRRQHYQRHDGFSEIVGEYIRSLGWSAAPAGEGDAFGLDFAIENPATG- 806 Query: 1002 RYLAGIECDGAAYHSSKSARDRDRLREEVLNDKGWQILRVWSTDWFDDPALQTDRLVAKI 1061 Y GIECD + + AR R+ R VL + RV S W+ D + RL A I Sbjct: 807 LYAIGIECDAPCHPLLERARAREIWRPSVLRRAIPHLHRVSSQGWYHDGDNERARLRAAI 866 Query: 1062 EALRARPTAVEDDYVVAKGSE 1082 E A P+A A+ S+ Sbjct: 867 EKALA-PSAETHPTAAAEASQ 886 >MPN153 [L] COG1112 Superfamily I DNA and RNA helicases and helicase subunits Mpn Mycoplasma pneumoniae Length = 1113 Score = 160 bits (406), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 125/443 (28%), Positives = 208/443 (46%), Gaps = 56/443 (12%) Query: 642 MSPLSLAKFMKAGSLS------FDMLVIDEASQMRPEDALGAMLRSKQIVVVGDQKQLPP 695 + P+ + F A L+ +D +VIDEASQ+ E A+ + R + ++ GD KQL P Sbjct: 674 LFPVHIMTFESAALLTPNQRRLYDYVVIDEASQVYLERAIPILYRGAKYIIAGDTKQLKP 733 Query: 696 TDFFARSGDGGSDEEDLDDYDDESILESCQKTFGLRR------PLRWHYRSRCESLIRFS 749 ++FF + DEE +D + E+ + S L+ L++HYRS +LI F+ Sbjct: 734 SNFFQARAEYDVDEE-FEDGNVEAAVHSTSLLHFLKNRSRILTLLKFHYRSDSANLIAFT 792 Query: 750 NERFYKRELITFPASKPSSFSIDLVR--VAGTFQARCNPVEASRVAE--EAITFMRHHAA 805 N R Y ELI F + + +V + G ++ N EA V + E +T + Sbjct: 793 NNRIYNNELI-FMNKATADKQVFIVHDVIDGIWRNNRNLQEARDVVQRLEQLTQTAEYQ- 850 Query: 806 LNESDVPSLGLVALNIQQRDLIQEEFNRLITDDVLVEQYREKVANRGEE--FFIKNLENV 863 SLG++ N Q +LI+ ++ ++ L+ ++R++V +GE F+KN+ENV Sbjct: 851 ------KSLGVICFNKNQAELIEYMIDK--QNNPLLNEWRDRVNAQGEYVGLFVKNIENV 902 Query: 864 QGDERDFVFISMTYGPEPGSTVLKQRFGPINRKQGHRRLNVLFSRARTRIGLFTSFSSSD 923 QGDERD + S+ Y S +GPI+++ G RLNV +RA+ RI LF + +SD Sbjct: 903 QGDERDIIIFSLGYDRSVNS------YGPISKQGGENRLNVAITRAKQRIELFKTNRASD 956 Query: 924 VRPTSDSAEGVHVLQKYLEYAETRGRMTTSPGGDGE--------PDSDFETEVADR---- 971 S ++ G +L +YL Y E M + G + P + +E E+ ++ Sbjct: 957 YNGLSSNSLGSKLLVEYLLYCEA---MANNQGESLDFQATQKQAPKAKYELELENQFFNE 1013 Query: 972 ---LRARGFTVDYQVGASGYKIDLGVRHPDFAERYLAGIECDGAAYHSSKSARDRDRLRE 1028 + FT+ V Y F E ++ + A HS K + RE Sbjct: 1014 LELIFGEQFTIKRNVNEGAYSFSFVFY---FNENPYLAVDFNPALPHSRKEVSENIIYRE 1070 Query: 1029 EVLNDKGWQILRVWSTDWFDDPA 1051 + L + W ++ +W +W +P Sbjct: 1071 QFLKKRKWNLVNIWLDEWKLNPG 1093