BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0143.0071.p (241 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0614 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 102 6e-22 AGl3303 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 87 2e-17 NMA1773 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 84 2e-16 NMB1584 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relat... 84 2e-16 PA2199 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and relate... 82 1e-15 >MA0614 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Mac Methanosarcina acetivorans str.C2A Length = 300 Score = 102 bits (255), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 64/223 (28%), Positives = 115/223 (51%), Gaps = 4/223 (1%) Query: 17 ADMVITVLKDGPAVDE---AMKAALPALRREVIWLQLGTVGIETVDALATFARKNGIIFF 73 AD+++T L D AV+ L ++ +W+ L T+ + A A+K G+ Sbjct: 66 ADIIMTSLTDEAAVNSVAFGEDGLLNGAKKGCLWIDLSTIDPSSSVKHAEAAKKAGLERL 125 Query: 74 DAPVQGTRQPAEQGKLVILASGPNKGRDVVQSVFDAIGQRTVWVANEPGASSRLKLALNS 133 D PV G++ A +G+L+IL G + + + +G+ +++ + G ++KLA+N Sbjct: 126 DTPVVGSKDLASKGELIILVGGSQEVLRKHEKFLNKLGKSVIYLGAD-GNGHKMKLAINL 184 Query: 134 FVFALTNGTAEALLIAKTLGVDPALVLETISGGPLDSGYFQTKGTAMLKGDFKTSFSVDN 193 + L +EAL+ ++ LG D +ETI+ P+ + Q KG +++G+F+ +FS++N Sbjct: 185 HLGLLAESFSEALVFSQKLGFDAKTFVETINNTPIRNYISQGKGPRIVEGNFEPAFSLNN 244 Query: 194 GVKDARLVVDAIAATGVCADVAKAGLVRFERTATAEYGGKDIA 236 KD RLV + I TG + K + + RT G KD + Sbjct: 245 LAKDLRLVNEQITKTGAILPMTKVSIEEYSRTVQNGEGQKDFS 287 >AGl3303 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 315 Score = 87.4 bits (215), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 61/197 (30%), Positives = 98/197 (49%), Gaps = 5/197 (2%) Query: 4 VEPVAEVVDAVTEADMVITVLKDGPAVDEAM---KAALPALRREVIWLQLGTVGIETVDA 60 V A +DA EAD+VI +L GP DE + + A++ + L + ++ +++ Sbjct: 55 VRQAATTIDAAVEADVVICMLSSGPVCDEVLLGPSGVVSAMKPGSVLLVMSSIPVDSARE 114 Query: 61 LATFARKNGIIFFDAPVQGTRQPAEQGKLVILASGPNKGRDVVQSVFDAIGQRTVWVANE 120 A AR +G+ + DAPV G + A +G L I+A G + D ++ + + +G+ V Sbjct: 115 QAQAARAHGVRYVDAPVSGGEKGAIEGTLAIMAGGEQRDVDALRPLLNCLGR--VTHVGP 172 Query: 121 PGASSRLKLALNSFVFALTNGTAEALLIAKTLGVDPALVLETISGGPLDSGYFQTKGTAM 180 G S KLA V + AEAL + + G DPA V + + GG +S F+ G M Sbjct: 173 VGCGSLAKLANQLIVASTICAVAEALTLVEAGGADPAQVRQALLGGFAESTVFRQHGKRM 232 Query: 181 LKGDFKTSFSVDNGVKD 197 ++GDF+ VKD Sbjct: 233 VEGDFRPGGPAKYQVKD 249 >NMA1773 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases NmA Neisseria meningitidis Z2491 Length = 289 Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 50/169 (29%), Positives = 83/169 (49%), Gaps = 1/169 (0%) Query: 72 FFDAPVQGTRQPAEQGKLVILASGPNKGRDVVQSVFDAIGQRTVWVANEPGASSRLKLAL 131 F +APV G+ PA G L+IL G + +Q +F +G++T + G S KL L Sbjct: 117 FAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFG-DVGKGSGAKLVL 175 Query: 132 NSFVFALTNGTAEALLIAKTLGVDPALVLETISGGPLDSGYFQTKGTAMLKGDFKTSFSV 191 NS + +EA+L+A+ G+D ++E I G +DS FQTK + +F +F++ Sbjct: 176 NSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFAL 235 Query: 192 DNGVKDARLVVDAIAATGVCADVAKAGLVRFERTATAEYGGKDIAATFL 240 + KD L V + G + + + A YG +D++ +L Sbjct: 236 KHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYL 284 >NMB1584 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Nme Neisseria meningitidis MC58 Length = 289 Score = 84.3 bits (207), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 50/169 (29%), Positives = 83/169 (49%), Gaps = 1/169 (0%) Query: 72 FFDAPVQGTRQPAEQGKLVILASGPNKGRDVVQSVFDAIGQRTVWVANEPGASSRLKLAL 131 F +APV G+ PA G L+IL G + +Q +F +G++T + G S KL L Sbjct: 117 FAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFG-DVGKGSGAKLVL 175 Query: 132 NSFVFALTNGTAEALLIAKTLGVDPALVLETISGGPLDSGYFQTKGTAMLKGDFKTSFSV 191 NS + +EA+L+A+ G+D ++E I G +DS FQTK + +F +F++ Sbjct: 176 NSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFAL 235 Query: 192 DNGVKDARLVVDAIAATGVCADVAKAGLVRFERTATAEYGGKDIAATFL 240 + KD L V + G + + + A YG +D++ +L Sbjct: 236 KHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYL 284 >PA2199 [I] COG2084 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases Pae Pseudomonas aeruginosa Length = 291 Score = 82.0 bits (201), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 56/200 (28%), Positives = 104/200 (52%), Gaps = 3/200 (1%) Query: 8 AEVVDAVTEADMVITVLKDGPAVDEAM--KAALPALRREVIWLQLGTVGIETVDALATFA 65 AE V + D+VI++L D A+ + AL R +I L + T+ ++ V+AL Sbjct: 47 AEEVGELFGLDVVISMLADDQAIRGVLLDSGALERARPGLIHLSMSTLSLDCVEALDQAH 106 Query: 66 RKNGIIFFDAPVQGTRQPAEQGKLVILASGPNKGRDVVQSVFDAIGQRTVWVANEPGASS 125 ++ G+ F APV G AE GKL I+ GP + + V+++ + +GQ+T + +P + Sbjct: 107 QRQGLAFVAAPVFGRTDVAEAGKLNIVVGGPEEAIEQVKALLEIMGQKTWFFGKDPRGAM 166 Query: 126 RLKLALNSFVFALTNGTAEALLIAKTLGVDPALVLETISGGPLDSGYFQTKGTAMLKGDF 185 +K++ N + + E++ + K LGV+P +E +S D+ ++ G +++ F Sbjct: 167 AVKISGNFMIASAIESMGESVALVKRLGVEPGRFMELMSSTLFDAPVYRNYGPQIVEQRF 226 Query: 186 KTS-FSVDNGVKDARLVVDA 204 + F + G+KD L + A Sbjct: 227 TPARFRLVLGLKDVDLALSA 246