BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0137.0020.p (262 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG1341G [IQR] COG1028 Dehydrogenases with different specificiti... 130 2e-30 BS_ykuF [IQR] COG1028 Dehydrogenases with different specificitie... 120 2e-27 PA2887 [IQR] COG1028 Dehydrogenases with different specificities... 120 4e-27 BH2144 [IQR] COG1028 Dehydrogenases with different specificities... 116 5e-26 SMc00268 [IQR] COG1028 Dehydrogenases with different specificiti... 108 1e-23 >VNG1341G [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Hbs Halobacterium sp. NRC-1 Length = 255 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 81/209 (38%), Positives = 118/209 (56%), Gaps = 9/209 (4%) Query: 41 CAR--XRLDALVDHLVHRGLSASAHVADIKDPDAVNAMFEALWTEHGRVDHLVNSAGGQF 98 C+R +DA+ D + G +A D+ D DAV+A+ EA E G +D LVN+AG F Sbjct: 38 CSRDHADVDAVADAIRTDGGTALPVECDVTDRDAVDALVEATVHEFGGLDVLVNNAGASF 97 Query: 99 PQPAIEVSIKGWNAVINTNLNGTWYMMQAAAQRWRDRKHPGSIVNIVVVT-THGLYGIAH 157 P ++S GW+ +I+ NL GT+ QAAA++ + R G++VN+ V G ++H Sbjct: 98 VAPFDDISRNGWDRIIDVNLGGTYNCTQAAAEQLK-RDGGGAVVNVASVAGQEGSPHMSH 156 Query: 158 SIAARSGVIGLSRALAVEWAPLNVRINCIAPGAIETEGWNVYSEAARAAYPRSNPMMRPG 217 AA++G+I L+R LA EWAP VR+NCIAPG + T G + + A R R G Sbjct: 157 YAAAKAGIITLTRTLAAEWAPAGVRVNCIAPGFVATPGLDAQMGVSAADIDRREVARRIG 216 Query: 218 TPWDIAE-----AAXSGKFITGETLTVDG 241 T +IA+ A+ + F+ GETLT G Sbjct: 217 TSAEIADAIRFLASPAASFVVGETLTAAG 245 >BS_ykuF [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 254 Score = 120 bits (302), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 75/187 (40%), Positives = 104/187 (55%), Gaps = 10/187 (5%) Query: 66 DIKDPDAVNAMFEALWTEHGRVDHLVNSAGGQFPQPAIEVSIKGWNAVINTNLNGTWYMM 125 D++ A + M + GR+D L+N+A G F PA +++ GW AVI LNGT++ Sbjct: 60 DVRSDSAASDMIKEAVKAFGRLDALINNAAGNFICPAEKLTPNGWKAVIEIVLNGTFFCS 119 Query: 126 QAAAQRWRDRKHPGSIVNIVVVTTHGL-YGIAHSIAARSGVIGLSRALAVEW-APLNVRI 183 QAAA+ W D+K G I+N+ G G+ HS AA++GV+ L+R LAVEW + +R Sbjct: 120 QAAARHWIDQKQQGVILNMAATYAWGAGAGVVHSAAAKAGVLSLTRTLAVEWGSKYGIRT 179 Query: 184 NCIAPGAIETEGWN---VYSEAARAAYPRSNPMMRPGTPWDIAEAAX-----SGKFITGE 235 N IAPG IE G SE A A S P+ R GTP +IA A +I G+ Sbjct: 180 NAIAPGPIERTGGAEKLFESEKAMARTMNSVPLGRLGTPEEIAALAAFLLSDEASYINGD 239 Query: 236 TLTVDGG 242 +T+DGG Sbjct: 240 CITMDGG 246 >PA2887 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 292 Score = 120 bits (300), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 76/225 (33%), Positives = 115/225 (51%), Gaps = 11/225 (4%) Query: 45 RLDALVDHLVHRGLSASAHVADIKDPDAVNAMFEALWTEHGRVDHLVNSAGGQFPQPAIE 104 +L+ +V G S S H DI++ +AV + + E G + HLVN+AGGQ+P P Sbjct: 50 KLEKTAGEIVEDGGSVSWHACDIREEEAVKTLVANILAERGTIHHLVNNAGGQYPSPLAS 109 Query: 105 VSIKGWNAVINTNLNGTWYMMQAAAQRWRDRKHPGSIVNIVVVTTHGLYGIAHSIAARSG 164 +S KG+ V+ TNL G + + + + K GSIVN++ G+ G+ HS AARSG Sbjct: 110 ISQKGFETVLRTNLVGGFLVAREVFNQSMS-KTGGSIVNMLADMWGGMPGMGHSGAARSG 168 Query: 165 VIGLSRALAVEWAPLNVRINCIAPGAIETEGWNVYSEAARAAYP---RSNPMMRPGTPWD 221 + +R AVEW VR+N +APG I + G + Y A +A P P+ R G+ + Sbjct: 169 MENFTRTAAVEWGHAGVRVNAVAPGWIASSGMDTYEGAFKAVIPTLREHVPLKRIGSESE 228 Query: 222 IAEA-----AXSGKFITGETLTVDGGGQHWGETW--TTGKPDYFR 259 +A A + F++G T+ +DG + GKP R Sbjct: 229 VAAAIVFLLSPGAAFVSGNTIRIDGAASQGSRAFPLFKGKPGQSR 273 >BH2144 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 257 Score = 116 bits (290), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 69/188 (36%), Positives = 106/188 (56%), Gaps = 12/188 (6%) Query: 66 DIKDPDAVNAMFEALWTEHGRVDHLVNSAGGQFPQPAIEVSIKGWNAVINTNLNGTWYMM 125 D+++PD V M + +E G +D LVN+A G F A ++S+ GW +VI+ LNGTW+ Sbjct: 60 DVRNPDDVERMVKDTLSEFGAIDGLVNNAAGNFLCAAEDLSLNGWKSVIDIVLNGTWHCT 119 Query: 126 QAAAQRWRDRKHPGSIVNIVVVT--THGLYGIAHSIAARSGVIGLSRALAVEWA-PLNVR 182 QA + W G I+N+V T G G+ HS +A++GV+ ++R LAVEW +R Sbjct: 120 QAVGKEWIKDGRKGHILNMVATYAWTAG-AGVVHSASAKAGVLAMTRTLAVEWGTKYGIR 178 Query: 183 INCIAPGAIETEGWN---VYSEAARAAYPRSNPMMRPGTPWDIAEAAX-----SGKFITG 234 +N IAPG IE G + +E + A ++ P+ R GT +IA A ++ G Sbjct: 179 LNAIAPGPIEGTGGAEKLILNETFQKAVLQTVPLKRFGTLEEIAGLAAYLFSEEATYMNG 238 Query: 235 ETLTVDGG 242 E +++DGG Sbjct: 239 ECISLDGG 246 >SMc00268 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 252 Score = 108 bits (270), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 68/187 (36%), Positives = 104/187 (55%), Gaps = 10/187 (5%) Query: 65 ADIKDPDAVNAMFEALWTEHGRVDHLVNSAGGQFPQPAIEVSIKGWNAVINTNLNGTWYM 124 ++ D +V + +AL ++ GR+D LVN+AG P +E++ + ++AV++ NL GT Y Sbjct: 63 CNVTDLASVRTVMDALVSQWGRIDILVNNAGITQPLKIMEIAPENYDAVLDVNLRGTLYC 122 Query: 125 MQAAAQRWRDRKHPGSIVNIVVVTTH---GLYGIAHSIAARSGVIGLSRALAVEWAPLNV 181 QA R RK G IVN+ V+ G++G H AA++GV+GL++A+A E AP NV Sbjct: 123 SQAVIPHMRSRKQ-GKIVNLSSVSAQRGGGIFGGPHYSAAKAGVLGLTKAMARELAPDNV 181 Query: 182 RINCIAPGAIETE-GWNVYSEAARAAYPRSNPMMRPGTPWDIAEAAXS-----GKFITGE 235 R+N I PG I T+ + + PM RPGT D+A A ++TG Sbjct: 182 RVNAICPGFIATDITGGLLTPEKLEEIKAGIPMGRPGTADDVAGCALFLASDLSAYVTGS 241 Query: 236 TLTVDGG 242 + V+GG Sbjct: 242 EVDVNGG 248