BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0134.0002.p (423 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_479 [E] COG0112 Glycine/serine hydroxymethyltransferase Aae A... 411 e-114 SA1915 [E] COG0112 Glycine/serine hydroxymethyltransferase Sau S... 409 e-114 BS_glyA [E] COG0112 Glycine/serine hydroxymethyltransferase Bsu ... 403 e-112 CAC2264 [E] COG0112 Glycine/serine hydroxymethyltransferase Cac ... 401 e-111 sll1931 [E] COG0112 Glycine/serine hydroxymethyltransferase Syn ... 397 e-110 >aq_479 [E] COG0112 Glycine/serine hydroxymethyltransferase Aae Aquifex aeolicus Length = 428 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 207/405 (51%), Positives = 270/405 (66%), Gaps = 3/405 (0%) Query: 12 DSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRYYGGQEFT 71 D EV A+ E RQ +E+I SEN+T V+E GSV TNKY+EG PG+RYYGG E+ Sbjct: 8 DPEVFDAVVKEYERQFYNLEMIASENFTSLAVMEATGSVLTNKYAEGLPGKRYYGGCEYV 67 Query: 72 DQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHGGHLTHGA 131 D +ENLA +RA LF AEHANVQP SGS N AVY +L PGDTI+ MDL+HGGHLTHGA Sbjct: 68 DVVENLAIERAKKLFGAEHANVQPHSGSQANMAVYFAVLNPGDTIMGMDLAHGGHLTHGA 127 Query: 132 PVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLDYASFKSI 191 V+ G+L+N Y P ID+D L +A+ +PKL++ G S+YPR D+A + I Sbjct: 128 KVNFSGKLYNVIHYGVNPET-ELIDYDQLYKLAKEHKPKLIVGGASAYPRVFDWAKMREI 186 Query: 192 ADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLCRREFAGK 251 ADEVGAL M D++HY GLIAG NP+ +T+T+HKTLRGPR G +L +E+A Sbjct: 187 ADEVGALFMVDMAHYAGLIAGGVYPNPVPYA-QFVTSTTHKTLRGPRSGFILTTKEYAKA 245 Query: 252 IDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALMERGMTLIT 311 +D SVFPG+QGGP M+V+A AV K+A + +F+ YA V+ NA+VLAE L + G ++T Sbjct: 246 VDKSVFPGIQGGPLMHVIAAKAVAFKEAMSEEFKEYAKQVVENARVLAEELKKYGFKIVT 305 Query: 312 GGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIRLGTPAAT 371 GGTD+H+++VD + + + G AE L+ GIT NK +P DP PP K SGIR+GT A T Sbjct: 306 GGTDSHIVLVD-LRNKNIIGKDAEKALEKAGITVNKNAVPFDPLPPTKTSGIRIGTAALT 364 Query: 372 TRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPV 416 TRGM E EMR IA I L + D ++R+R E+C FP+ Sbjct: 365 TRGMKEDEMRKIAGWINEVLSNMDDEKTIQRVRQEVRELCETFPL 409 >SA1915 [E] COG0112 Glycine/serine hydroxymethyltransferase Sau Staphylococcus aureus N315 Length = 412 Score = 409 bits (1051), Expect = e-114, Method: Compositional matrix adjust. Identities = 199/405 (49%), Positives = 270/405 (66%), Gaps = 2/405 (0%) Query: 12 DSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRYYGGQEFT 71 D +A A+E E +RQ +ELI SEN+ V+E GSV TNKY+EGYPGRRYYGG EF Sbjct: 8 DKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQGSVLTNKYAEGYPGRRYYGGCEFV 67 Query: 72 DQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHGGHLTHGA 131 D E++A RA ALF AEH NVQP SGS N AVYL L+ GDT+L M+LSHGGHLTHGA Sbjct: 68 DVTESIAIDRAKALFGAEHVNVQPHSGSQANMAVYLVALEMGDTVLGMNLSHGGHLTHGA 127 Query: 132 PVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLDYASFKSI 191 PV+ G+ +NF Y + I++D +R +A +PKL++ G S+Y R +D+ FK I Sbjct: 128 PVNFSGKFYNFVEYGVD-KDTERINYDEVRKLALEHKPKLIVAGASAYSRTIDFKKFKEI 186 Query: 192 ADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLCRREFAGK 251 ADEV A M D++H GL+A NP++ D +TTT+HKTLRGPRGG++LC+ E+ Sbjct: 187 ADEVNAKLMVDMAHIAGLVAAGLHPNPVEYA-DFVTTTTHKTLRGPRGGMILCKEEYKKD 245 Query: 252 IDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALMERGMTLIT 311 ID ++FPG+QGGP +V+A AV +A ++F+ Y V++NAKVLAEAL+ G +++ Sbjct: 246 IDKTIFPGIQGGPLEHVIAAKAVAFGEALENNFKTYQQQVVKNAKVLAEALINEGFRIVS 305 Query: 312 GGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIRLGTPAAT 371 GGTDNH++ VD S+GL G AE LD+VGIT NK IP D P SGIRLGTPAAT Sbjct: 306 GGTDNHLVAVDVKGSIGLTGKEAEETLDSVGITCNKNTIPFDQEKPFVTSGIRLGTPAAT 365 Query: 372 TRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPV 416 TRG E +A++I+ +L++ D +++ + ++ +P+ Sbjct: 366 TRGFDEKAFEEVAKIISLALKNSKDEEKLQQAKERVAKLTAEYPL 410 >BS_glyA [E] COG0112 Glycine/serine hydroxymethyltransferase Bsu Bacillus subtilis Length = 415 Score = 403 bits (1035), Expect = e-112, Method: Compositional matrix adjust. Identities = 200/406 (49%), Positives = 268/406 (66%), Gaps = 3/406 (0%) Query: 11 NDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRYYGGQEF 70 D +V A++ E RQQ +ELI SEN+ V+E GSV TNKY+EGYPG+RYYGG E Sbjct: 7 QDEQVFNAIKNERERQQTKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEH 66 Query: 71 TDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHGGHLTHG 130 D +E++AR RA +F AEH NVQP SG+ N AVY +L+ GDT+L M+LSHGGHLTHG Sbjct: 67 VDVVEDIARDRAKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHG 126 Query: 131 APVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLDYASFKS 190 +PV+ G +NF Y ID+D +R A +PKL++ G S+YPR +D+ F+ Sbjct: 127 SPVNFSGVQYNFVEYGVDKET-QYIDYDDVREKALAHKPKLIVAGASAYPRTIDFKKFRE 185 Query: 191 IADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLCRREFAG 250 IADEVGA M D++H GL+A NP+ D +TTT+HKTLRGPRGG++LCR EF Sbjct: 186 IADEVGAYFMVDMAHIAGLVAAGLHPNPVPYA-DFVTTTTHKTLRGPRGGMILCREEFGK 244 Query: 251 KIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALMERGMTLI 310 KID S+FPG+QGGP M+V+A AV+ + DF+ YA VI NAK LAEAL + G+ L+ Sbjct: 245 KIDKSIFPGIQGGPLMHVIAAKAVSFGEVLQDDFKTYAQNVISNAKRLAEALTKEGIQLV 304 Query: 311 TGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIRLGTPAA 370 +GGTDNH+++VD + S+GL G AE++LD +GIT+NK IP DP P SGIRLGT A Sbjct: 305 SGGTDNHLILVD-LRSLGLTGKVAEHVLDEIGITSNKNAIPYDPEKPFVTSGIRLGTAAV 363 Query: 371 TTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPV 416 T+RG + + +IA +L++ D ++ R + FP+ Sbjct: 364 TSRGFDGDALEEVGAIIALALKNHEDEGKLEEARQRVAALTDKFPL 409 >CAC2264 [E] COG0112 Glycine/serine hydroxymethyltransferase Cac Clostridium acetobutylicum Length = 411 Score = 401 bits (1031), Expect = e-111, Method: Compositional matrix adjust. Identities = 193/413 (46%), Positives = 273/413 (66%), Gaps = 6/413 (1%) Query: 4 FRNELHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRR 63 F N + ++DSEV +E E RQ++ +ELI SEN+T V+E +GS TNKY+EGYPG+R Sbjct: 3 FEN-IKVSDSEVYSIIEEENARQENNIELIASENFTSKAVMEAMGSYLTNKYAEGYPGKR 61 Query: 64 YYGGQEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSH 123 YYGG D++E LAR+RA LF+AEHANVQP SGS N AVY +L+PGDTI+ M+L+ Sbjct: 62 YYGGCYVVDKVEELARERAKKLFKAEHANVQPHSGSQANMAVYFAVLKPGDTIMGMNLTD 121 Query: 124 GGHLTHGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDL 183 GGHLTHG+PV+ G+LFN Y ID++ R A +PK+++ G S+Y R + Sbjct: 122 GGHLTHGSPVNFSGKLFNIIAYGVSDET-EQIDYEAFRKKALECKPKMIVSGASAYSRII 180 Query: 184 DYASFKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVL 243 D+ + I DEVGA M D++H GL+A +P+ D +TTT+HKTLRGPRGG + Sbjct: 181 DFKKIREICDEVGAYMMVDMAHIAGLVAAGLHPSPIPYA-DFVTTTTHKTLRGPRGGAIF 239 Query: 244 CRREFAGKIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALM 303 C+ ++A ID SVFPG+QGGP M+++AG AV +A DF+ YA ++ NAKV A+ L Sbjct: 240 CKEKYAKDIDKSVFPGMQGGPLMHIIAGKAVCFGEALKDDFKDYAQQIVNNAKVFADELT 299 Query: 304 ERGMTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGI 363 + G +++GGTDNH+++VD + + + G AE++LD+VGITANK IP + + P SGI Sbjct: 300 KYGFRIVSGGTDNHLLLVD-LTNKNITGKDAEHLLDSVGITANKNTIPFEKKSPFITSGI 358 Query: 364 RLGTPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPV 416 R+GTP+ TTRG E EM+ +A I + + + + +R E+C FP+ Sbjct: 359 RMGTPSVTTRGFKEEEMKKVAYFINYVIEHRDED--LSEIRKQVSELCSGFPI 409 >sll1931 [E] COG0112 Glycine/serine hydroxymethyltransferase Syn Synechocystis Length = 427 Score = 397 bits (1020), Expect = e-110, Method: Compositional matrix adjust. Identities = 204/410 (49%), Positives = 269/410 (65%), Gaps = 4/410 (0%) Query: 8 LHLNDSEVALALEGEERRQQDGVELIPSENYTYPEVLELLGSVFTNKYSEGYPGRRYYGG 67 L +D +A ++ E +RQ+ +ELI SEN+T V+ GSV TNKY+EG PG+RYYGG Sbjct: 9 LATSDPALAAIIDRELQRQRTHIELIASENFTSAAVMAAQGSVLTNKYAEGLPGKRYYGG 68 Query: 68 QEFTDQIENLARQRACALFRAEHANVQPLSGSPMNQAVYLGLLQPGDTILAMDLSHGGHL 127 EF DQ E LA R LF A HANVQP SG+ N AV+L LLQPGDTI+ MDLSHGGHL Sbjct: 69 CEFVDQAETLAISRVKELFGAAHANVQPHSGAQANFAVFLTLLQPGDTIMGMDLSHGGHL 128 Query: 128 THGAPVSHMGRLFNFARYKTQPSNGGAIDFDGLRAMARHVRPKLVLCGYSSYPRDLDYAS 187 THG+PV+ G+ F A Y + G +D+D +R A V+PKL++CGYS+YPR +++ Sbjct: 129 THGSPVNVSGKWFEVAHYGVEKET-GRLDYDKIRQQALEVKPKLLICGYSAYPRQIEFDK 187 Query: 188 FKSIADEVGALTMADVSHYGGLIAGNALRNPLDAGFDVMTTTSHKTLRGPRGGIVLCRRE 247 F++IADEVGA MAD++H GL+A +PL DV+TTT+HKTLRGPRGG+++ E Sbjct: 188 FRAIADEVGAYLMADIAHIAGLVASGHHPSPLPY-CDVVTTTTHKTLRGPRGGLIMTNNE 246 Query: 248 FAG-KIDASVFPGLQGGPHMNVVAGTAVTLKKASTSDFQAYAGLVIRNAKVLAEALMERG 306 G K D SVFPG QGGP +V+ AV +A +F+ Y+G VI NA+ +A+ L +RG Sbjct: 247 ELGKKFDKSVFPGTQGGPLEHVITAKAVAFGEALKPEFKVYSGQVIANAQAMADQLQKRG 306 Query: 307 MTLITGGTDNHMMVVDTVASVGLDGGAAENILDAVGITANKQIIPDDPRPPLKPSGIRLG 366 L++GGTDNH+M+VD + S+ + G + +L + ITANK +P DP P SG+RLG Sbjct: 307 FDLVSGGTDNHLMLVD-LRSIAMTGKVGDQLLGEINITANKNTVPFDPESPFVTSGLRLG 365 Query: 367 TPAATTRGMGEPEMRLIAELIAGSLRSKGDAAVVKRLRSNSVEMCRAFPV 416 +PA TTRGM E E R IA +IA L S D V E+C FP+ Sbjct: 366 SPAMTTRGMQEDEFRTIANIIADRLLSPEDEGVKADCLRRVSELCAGFPL 415