BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0132.0040.p (442 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF0333 [Q] COG2368 Aromatic ring hydroxylase Afu Archaeoglobus f... 335 1e-91 AF1027 [Q] COG2368 Aromatic ring hydroxylase Afu Archaeoglobus f... 311 3e-84 PAE2693 [Q] COG2368 Aromatic ring hydroxylase Pya Pyrobaculum ae... 249 7e-66 SSO2738 [Q] COG2368 Aromatic ring hydroxylase Sso Sulfolobus sol... 237 5e-62 SSO2503 [Q] COG2368 Aromatic ring hydroxylase Sso Sulfolobus sol... 213 5e-55 >AF0333 [Q] COG2368 Aromatic ring hydroxylase Afu Archaeoglobus fulgidus Length = 500 Score = 335 bits (860), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 180/444 (40%), Positives = 266/444 (59%), Gaps = 15/444 (3%) Query: 2 LMTAADYRESLRRYKPRVFVNGAAVASVADEPLLAPGVAGVGVTYDFALQPEHVPIMTAR 61 +M +Y ESLR+ KP+V+ G + SVAD+P+ AP V +TY+ A P + +MTA Sbjct: 4 MMNGKEYIESLRKLKPKVYFMGRKIDSVADDPITAPHVNAAAMTYELANDPRYEDVMTAT 63 Query: 62 QGTSGKTVNRMLHINRSSQDLLDKLEAVRLVCRTSG-CAQRYLSHDALNGLYQATRLTDD 120 +G+ +NR HI++S++DL+ K+ +RL+ R +G C QR + DAL LY T D Sbjct: 64 SHLTGEKINRFTHIHQSTEDLVKKVRMMRLLGRKTGSCFQRCVGLDALCALYITTYDVDR 123 Query: 121 RHGTDYSQRFLNYLHEVQDQDLTLGVAMTDAKGDRSKRPGLQANTDVYVHIRERRPDGIV 180 ++GTDY +RF +YL VQ DL AMTD KGDRS RP QA+ DVYV + ERR DGIV Sbjct: 124 KYGTDYHERFKDYLIHVQKNDLMAAGAMTDVKGDRSLRPHQQADPDVYVRVVERREDGIV 183 Query: 181 IRGTKAIVTGAPYMHEFLVMPCRTHVPADKDFAVCCAVPVDAPGVTIVARPAGRPGDAAA 240 +RG KA +TGA HE + +P R DKD+AV AVPVDA GV ++ GR + Sbjct: 184 VRGAKAHITGAVNSHEIIALPTRAMGEEDKDYAVAFAVPVDAEGVVMI---FGRQTNDVR 240 Query: 241 KF-------SAKYGQSVG--VVMFDDVFVPHDRVFLAGETEEGGFLTTSYATHHRHSCIG 291 + +AKY G +++F+DVFVP +RVF+ GE + L ++AT+HR + G Sbjct: 241 RLDGDIDCGNAKYATVGGEALIIFNDVFVPWERVFMCGEYDFASELVETFATYHRQNYGG 300 Query: 292 ARAGFGDLLIGAGALMIEANGLDAEKHAHIREAMVELITITESFYACGVASSVYCTKDPA 351 + G D+LIGA A + E NG+ E+ +HI + + E++ + E+ + C +A S K P+ Sbjct: 301 CKVGVADVLIGASAAIAEYNGV--ERASHIVDKLTEMVHLAETCWCCSLACSYEGFKTPS 358 Query: 352 GSVMPDAVFSNIGKLLLATKIYDMHRVAHYVSGGLIVALPGPDEDHNPETRASLAAVMGG 411 G+ MP+ + +N+ KL + Y+ R+A + GGL+ P + NPETR + + G Sbjct: 359 GAYMPNLLLANVTKLNITRFPYEWSRLAQDIVGGLVATTPSELDYKNPETRDLIEKYLKG 418 Query: 412 RPDIPAEQRAEVARLIEDLTVSHE 435 + D+P E R + RLIE+L++ E Sbjct: 419 KADVPTEHRVRMIRLIENLSIGAE 442 >AF1027 [Q] COG2368 Aromatic ring hydroxylase Afu Archaeoglobus fulgidus Length = 480 Score = 311 bits (796), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 171/420 (40%), Positives = 240/420 (57%), Gaps = 6/420 (1%) Query: 2 LMTAADYRESLRRYKPRVFVNGAAVASVADEPLLAPGVAGVGVTYDFALQPEHVPIMTAR 61 + T+ +YR+ L + KP V+V G V D P ++ G+ + T+D P++ ++T + Sbjct: 1 MRTSEEYRQDLYKLKPNVYVRGKKVRR--DSPEVSGGINVISKTFDLVEHPDYKDLLTTK 58 Query: 62 QGTSGKTVNRMLHINRSSQDLLDKLEAVRLVCR-TSGCAQRYLSHDALNGLYQATRLTDD 120 SGK +NR HIN+S++DLL K R +CR GC QR + DA+NGL AT D Sbjct: 59 SHISGKRINRFTHINQSAEDLLKKQLMTRKLCRLVGGCIQRCMGCDAINGLSVATFAADT 118 Query: 121 RHGTDYSQRFLNYLHEVQDQDLTLGVAMTDAKGDRSKRPGLQANTDVYVHIRERRPDGIV 180 HGTDY +RFL Y+ E Q++DL A TD KGDRSKRP Q + D+YV + ERR DGIV Sbjct: 119 EHGTDYHKRFLEYVKEFQERDLVAACAQTDVKGDRSKRPHQQDDPDMYVRVVERRSDGIV 178 Query: 181 IRGTKAIVTGAPYMHEFLVMPCRTHVPADKDFAVCCAVPVDAPGVTIVARPAGRPGDAAA 240 +RG K +T A E +V+P R P DKD++V AVP D GV IVAR + Sbjct: 179 VRGAKNCITMAAVADEIIVVPTRAMTPEDKDYSVAFAVPADTEGVKIVARTSKLRAPEGL 238 Query: 241 KF-SAKYGQSVGVVMFDDVFVPHDRVFLAGETEEGGFLTTSYATHHRHSCIGARAGFGDL 299 K + G +++FDDVFVP DRVFL GE + +A HRHS G + D+ Sbjct: 239 KMPQGEIGDDENMIIFDDVFVPWDRVFLCGENKYATLAAHLFALFHRHSYTGCKPATTDM 298 Query: 300 LIGAGALMIEANGLDAEKHAHIREAMVELITITESFYACGVASSVYCTKDPAGSVMPDAV 359 ++G GAL+ E NG+ EKH +IRE +VEL E +A GVA++ Y K PAG+ +P+ V Sbjct: 299 IMGFGALIAEYNGV-YEKH-NIREKLVELAATAELVFAAGVAAAHYGQKSPAGTFIPNEV 356 Query: 360 FSNIGKLLLATKIYDMHRVAHYVSGGLIVALPGPDEDHNPETRASLAAVMGGRPDIPAEQ 419 ++N+G+ Y + +SGGL ALP ++ + E + + R D+PAE Sbjct: 357 YANVGRRHAGLNYYKELEILAELSGGLPAALPFAEDFLSEEVGPYIRKYIKRRADVPAEH 416 >PAE2693 [Q] COG2368 Aromatic ring hydroxylase Pya Pyrobaculum aerophilum Length = 500 Score = 249 bits (637), Expect = 7e-66, Method: Compositional matrix adjust. Identities = 152/429 (35%), Positives = 223/429 (51%), Gaps = 20/429 (4%) Query: 2 LMTAADYRESLRRYK-PRVFVNGAAVASVADEPLLAPGVAGVGVTYDFALQPEHVPIMTA 60 L T Y E LR+ K ++V G V V P L P V T+D A + + A Sbjct: 3 LRTGDQYVEGLRKRKHVEIYVMGKRVEDVTAHPFLKPSVKSFKATFDAAFDEDTRGLARA 62 Query: 61 RQGTSGKTVNRMLHINRSSQDLLDKLEAVR-LVCRTSGCAQRYLSHDALNGLYQATRLTD 119 G+ VNR +HI++ +DL+ K++ +R L +T C QR + DALN LY T Sbjct: 63 YSPFIGEVVNRFVHIHQGPEDLVAKVKLLRKLSHKTGTCFQRCVGWDALNTLYIITGKIA 122 Query: 120 DRHGT-DYSQRFLNYLHEVQDQDLTLGVAMTDAKGDRSKRPGLQANTDVYVHIRERRPDG 178 +R G +Y +RF+ YL VQ DL L AMTD KG R+ RP Q N D YV + E R DG Sbjct: 123 EREGRREYVERFIEYLKYVQKNDLALAGAMTDVKGVRTLRPSEQPNKDAYVRVVEEREDG 182 Query: 179 IVIRGTKAIVTGAPYMHEFLVMPCRTHVPADKDFAVCCAVPVDAPGVTIVARPAGRPGDA 238 IV+RG KA +TG + E +VMP R D ++A+ A+P+D PGV +V GR + Sbjct: 183 IVVRGAKANITGIAVVDEVIVMPTRAMTEKDAEYAISFAIPLDTPGVKVV---VGRQLND 239 Query: 239 AAKFSAKYGQSV-------GVVMFDDVFVPHDRVFLAGETEEGGFLTTSYATHHRHSCIG 291 + +F + G ++F+DVFVP +RVF E G ++ +HR G Sbjct: 240 SRRFEEGEIDGLPYMFNHEGFLIFEDVFVPWERVFFYKEWRWSGVFVEVFSNYHRQGYAG 299 Query: 292 ARAGFGDLLIGAGALMIEANGLDAEKHAHIREAMVELITITESFYACGVASSVYCTK--- 348 ++G GD++IGA + A L HI+E + E+ + E+ Y+ G+A+S K Sbjct: 300 CKSGLGDVIIGASYGL--ARRLGVADKPHIQEKLTEMAFLNETMYSAGLAASWEARKLLR 357 Query: 349 DPAGSVMPDAVFSNIGKLLLATKIYDMHRVAHYVSGGLIVALPGPDEDHNPETRASLAAV 408 D V P +++N+ K L+ Y++ R+AH ++GGL+ P + NPE R L Sbjct: 358 DGGWWVNP--MYANVTKHLVTRFPYEISRLAHDIAGGLLGTAPSEADFKNPELRPLLEKY 415 Query: 409 MGGRPDIPA 417 + G P+ PA Sbjct: 416 LQGVPEFPA 424 >SSO2738 [Q] COG2368 Aromatic ring hydroxylase Sso Sulfolobus solfataricus Length = 502 Score = 237 bits (604), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 145/442 (32%), Positives = 232/442 (52%), Gaps = 15/442 (3%) Query: 1 MLMTAADYRESLR-RYKPRVFVNGAAVASVADEPLLAPGVAGVGVTYDFALQPEHVPIMT 59 +L T +Y ++R R K ++V G V V + P L P V TYD A + + + Sbjct: 2 VLRTGEEYVNAIRHRNKVEIYVMGKEVKDVTEHPFLKPSVLAFKATYDAAHEEDSESLAR 61 Query: 60 ARQGTSGKTVNRMLHINRSSQDLLDKLEAVRLVC-RTSGCAQRYLSHDALNGLYQATRLT 118 A + VNR HI+RS +DL K++ +R + +T C QR + DALN +Y +RL Sbjct: 62 AWSSFINEEVNRFTHIHRSPEDLAAKVKLLRKISHKTGACFQRCVGWDALNAIYVTSRLM 121 Query: 119 DDRHGTDYSQRFLNYLHEVQDQDLTLGVAMTDAKGDRSKRPGLQANTDVYVHIRERRPDG 178 +R +Y +RF+ YL VQ D+ L AMTD KG R+ RP Q + Y+ + + DG Sbjct: 122 AERGKKEYYERFVEYLKYVQKNDVALAGAMTDVKGIRTLRPHEQPHA--YLRVTQVTKDG 179 Query: 179 IVIRGTKAIVTGAPYMHEFLVMPCRTHVPADKDFAVCCAVPVDAPGV-TIVARPA--GRP 235 I + G KA +TG E +VMP R D DFAV ++P+D G+ I+ R GR Sbjct: 180 IYVSGAKANITGIGAAEEIVVMPTRAMSEKDMDFAVAFSIPLDTEGIKVIIGRQVNDGRR 239 Query: 236 GDAAAKFSAKYGQS-VGVVMFDDVFVPHDRVFLAGETEEGGFLTTSYATHHRHSCIGARA 294 + Y + G+V+FD+VFVP +RVF+ G+ + G L ++++HR G ++ Sbjct: 240 LEEGEIDGLPYRTNHEGLVIFDNVFVPMERVFMLGDWQMSGILVEIFSSYHRQGYGGCKS 299 Query: 295 GFGDLLIGAGALMIEANGLDAEKHAHIREAMVELITITESFYACGVASSVYCTK---DPA 351 G GD++IGA + A + EK HI+ + E++ + E+ Y G+A+S+ K D Sbjct: 300 GLGDVIIGAAYNL--AKQIGVEKAPHIQNKLNEMVLLNENMYGSGIAASLDAVKLCEDGC 357 Query: 352 GSVMPDAVFSNIGKLLLATKIYDMHRVAHYVSGGLIVALPGPDEDHNPETRASLAAVMGG 411 V P +F+N+ K L+ Y++ + + ++GG+I P + NP+ + + + G Sbjct: 358 WWVNP--MFANVTKQLVTRFPYEITKFSTDIAGGIIGTAPSEWDLKNPKLKPYIEKYLQG 415 Query: 412 RPDIPAEQRAEVARLIEDLTVS 433 D E R + RL+E++ S Sbjct: 416 MMDYSTEDRLRMVRLLENVGFS 437 >SSO2503 [Q] COG2368 Aromatic ring hydroxylase Sso Sulfolobus solfataricus Length = 463 Score = 213 bits (543), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 122/374 (32%), Positives = 206/374 (55%), Gaps = 10/374 (2%) Query: 69 VNRMLHINRSSQDLLDKLEAV---RLVCRTSGCAQRYLSHDALNGLYQATRLTDDRHGTD 125 +++ + +SQDLLD+ + + + C + + DA+ L + D ++GTD Sbjct: 59 MSKFFKVPWTSQDLLDRHKLIYDLTMYCNGVFNISQAIGSDAIFALMITAKQVDRKYGTD 118 Query: 126 YSQRFLNYLHEVQDQDLTLGVAMTDAKGDRSKRPGLQANTDVYVHIRERRPDGIVIRGTK 185 YS+R Y V +DLTL A TD KGDRSKRP Q + D+YV + + + DGIV+RG K Sbjct: 119 YSKRVEKYFERVAKEDLTLATAQTDVKGDRSKRPSEQVDPDMYVRVVDVKSDGIVVRGAK 178 Query: 186 AIVTGAPYMHEFLVMPCRTHVPADKDFAVCCAVPVDAPGVTIVARPAGR-PGDAAAKFSA 244 A T + E +V+P R +DKD+AV AVP + G+ + RP G++++ S Sbjct: 179 AHTTQSAVSDEIIVIPTRVMRDSDKDYAVAFAVPANTKGLKMYIRPIDEIEGNSSSVLSR 238 Query: 245 KYGQSVGVVMFDDVFVPHDRVFLAGETEEGGFLTTSYATHHRHSCIGARAGFGDLLIGAG 304 K + + +F+DVFVP DRVFL E + G L +AT HR + + R+ +L +GA Sbjct: 239 KDYELETLTVFNDVFVPWDRVFLFKEYDYAGTLAMLFATFHRFTALSYRSATMNLYLGAS 298 Query: 305 ALMIEANGLDAEKHAHIREAMVELITITESFYACGVASSVYCTKDPAGSVMPDAVFSNIG 364 + + NG++ EK H+R+ +V++I E + +A++VY + G +P+ +F+N+G Sbjct: 299 KVASQVNGIENEK--HVRDDIVDIILYKEIMRSSAIAAAVYPV-NMEGIAVPNPLFTNVG 355 Query: 365 KLLLATKIYDMHRVAHYVSGGLIVALPGPDEDHNPETRASLAAVMGGRPDIPAEQRAEVA 424 KL +D+ R ++GG+I +P ++ + E + + + G + E+RA+V Sbjct: 356 KLYSNMHFHDVVRDLIDIAGGIIATMPSQEDLESDEGKNIVKYLRGS---VDGEERAKVL 412 Query: 425 RLIEDLTVSHEAGW 438 +L ++L S G+ Sbjct: 413 KLAKELGASTFTGY 426