BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0129.0061.p (346 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1087 Non COG 391 e-108 Z3393 [Q] COG3435 Gentisate 1,2-dioxygenase EcZ Escherichia coli... 384 e-106 ECs3030 [Q] COG3435 Gentisate 1,2-dioxygenase Ecs Escherichia co... 384 e-106 STM2178 [Q] COG3435 Gentisate 1,2-dioxygenase Sty Salmonella typ... 380 e-105 PA2470 [Q] COG3435 Gentisate 1,2-dioxygenase Pae Pseudomonas aer... 378 e-105 >RSc1087 Non COG Length = 348 Score = 391 bits (1004), Expect = e-108, Method: Compositional matrix adjust. Identities = 183/347 (52%), Positives = 247/347 (71%), Gaps = 4/347 (1%) Query: 1 MEAVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGK 60 M + PER+ +Y + G L+ LW ++ L+ EPK P W++ IR + +AG+ Sbjct: 1 MTSQTLPPERDTYYSLLGGMGLAPLWESLHALVPREPKPRIVPAHWQYAAIRPLVMQAGR 60 Query: 61 LITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKG 120 +I+A+EA RRVLVLENPGL G+S IT++L+AG+Q+++PG+IAP+HRH QSALRF++EGKG Sbjct: 61 IISAEEAVRRVLVLENPGLPGRSSITSTLYAGLQLILPGEIAPSHRHTQSALRFIVEGKG 120 Query: 121 AFTAVDGERTAMSPGDFVITPSMTWHDHS----NETNEPMFWLDGLDIPMVQFFDCSFAE 176 A+TAVDGERT M PGDF+ITPS TWHDH +E EP+ WLDGLDIP+VQ FD FAE Sbjct: 121 AWTAVDGERTTMHPGDFIITPSWTWHDHGCPRLDEGGEPVVWLDGLDIPLVQQFDAGFAE 180 Query: 177 GAKEDQQSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWD 236 E QQ +++P G SFAR+GHN++P+ + + TSP+F+YPYE +REAL+ E D Sbjct: 181 NHPEAQQPVTRPEGDSFARFGHNMVPVRHRVSDPTSPVFSYPYERSREALDTLYRHGELD 240 Query: 237 ACHGLKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQ 296 G+KL++ NP TG + MPTI TF+Q LP GF+ YR+TDATV+ +EG+G +G+ Sbjct: 241 PWDGVKLRYVNPATGGWPMPTIATFMQYLPAGFRGKTYRSTDATVYCVVEGRGTAHIGEA 300 Query: 297 TFEWGPRDLFVVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDR 343 F + P D+FVVPSW V +A D+VLFS+SDRPV L+L RE R Sbjct: 301 RFAFAPHDVFVVPSWAPVRLDAQDDAVLFSYSDRPVLAALNLLREAR 347 >Z3393 [Q] COG3435 Gentisate 1,2-dioxygenase EcZ Escherichia coli O157:H7 EDL933 Length = 342 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 177/337 (52%), Positives = 242/337 (71%), Gaps = 7/337 (2%) Query: 10 REAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKLITAKEAER 69 RE FY+ I GQNL+ LW ++ L+ P + C P W + +IR + E+G LI AKEA R Sbjct: 9 REQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAYWNYQEIRPLLLESGGLIGAKEAVR 68 Query: 70 RVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGAFTAVDGER 129 RVLVLENP LRGQS IT +L+AG+Q+++PG++AP+HRH QSALRF++EGKGAFTAVDGER Sbjct: 69 RVLVLENPALRGQSSITATLYAGLQLIMPGEVAPSHRHNQSALRFIVEGKGAFTAVDGER 128 Query: 130 TAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKEDQQSISKPA 189 T M+ GDF++TP WHDH N +EP+ WLDGLD+P+V C FAE E+QQ +++ Sbjct: 129 TPMNEGDFILTPQWRWHDHGNPGDEPVIWLDGLDLPLVNILGCGFAEDYPEEQQPVTRKE 188 Query: 190 GASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREAL-EKAKVG--DEWDACHGLKLKFS 246 G RY N+LP+ + +T +SPIFNY Y+ +RE L + ++G DEWD G K+++ Sbjct: 189 GDYLPRYAANMLPL-RHQTGNSSPIFNYRYDRSREVLHDLTRLGDADEWD---GYKMRYV 244 Query: 247 NPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWGPRDLF 306 NP TG + MP++G F+QLLPKGF + R TD+T++ +EG G+ +G++TF + +D+F Sbjct: 245 NPVTGGYPMPSMGAFLQLLPKGFASRVARTTDSTIYHVVEGSGQVIIGNETFSFSAKDIF 304 Query: 307 VVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDR 343 VVP+WH V+ + DSVLFSFSDRPVQ+ L LFRE R Sbjct: 305 VVPTWHGVSFQTTQDSVLFSFSDRPVQEALGLFREAR 341 >ECs3030 [Q] COG3435 Gentisate 1,2-dioxygenase Ecs Escherichia coli O157:H7 Length = 342 Score = 384 bits (986), Expect = e-106, Method: Compositional matrix adjust. Identities = 177/337 (52%), Positives = 242/337 (71%), Gaps = 7/337 (2%) Query: 10 REAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKLITAKEAER 69 RE FY+ I GQNL+ LW ++ L+ P + C P W + +IR + E+G LI AKEA R Sbjct: 9 REQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAYWNYQEIRPLLLESGGLIGAKEAVR 68 Query: 70 RVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGAFTAVDGER 129 RVLVLENP LRGQS IT +L+AG+Q+++PG++AP+HRH QSALRF++EGKGAFTAVDGER Sbjct: 69 RVLVLENPALRGQSSITATLYAGLQLIMPGEVAPSHRHNQSALRFIVEGKGAFTAVDGER 128 Query: 130 TAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKEDQQSISKPA 189 T M+ GDF++TP WHDH N +EP+ WLDGLD+P+V C FAE E+QQ +++ Sbjct: 129 TPMNEGDFILTPQWRWHDHGNPGDEPVIWLDGLDLPLVNILGCGFAEDYPEEQQPVTRKE 188 Query: 190 GASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREAL-EKAKVG--DEWDACHGLKLKFS 246 G RY N+LP+ + +T +SPIFNY Y+ +RE L + ++G DEWD G K+++ Sbjct: 189 GDYLPRYAANMLPL-RHQTGNSSPIFNYRYDRSREVLHDLTRLGDADEWD---GYKMRYV 244 Query: 247 NPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWGPRDLF 306 NP TG + MP++G F+QLLPKGF + R TD+T++ +EG G+ +G++TF + +D+F Sbjct: 245 NPVTGGYPMPSMGAFLQLLPKGFASRVARTTDSTIYHVVEGSGQVIIGNETFSFSAKDIF 304 Query: 307 VVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDR 343 VVP+WH V+ + DSVLFSFSDRPVQ+ L LFRE R Sbjct: 305 VVPTWHGVSFQTTQDSVLFSFSDRPVQEALGLFREAR 341 >STM2178 [Q] COG3435 Gentisate 1,2-dioxygenase Sty Salmonella typhimurium LT2 Length = 345 Score = 380 bits (975), Expect = e-105, Method: Compositional matrix adjust. Identities = 173/337 (51%), Positives = 241/337 (71%), Gaps = 7/337 (2%) Query: 10 REAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGKLITAKEAER 69 R+ +Y+ I GQNL+ LW ++ L+ P + C P W + +IR + E+G +I AKEA R Sbjct: 12 RQQYYQHISGQNLTPLWESLHHLVPQTPNANCAPAYWNYQEIRPLLMESGNVIGAKEAIR 71 Query: 70 RVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKGAFTAVDGER 129 RVLVLENP LRGQS IT +L+AG+Q+++PG++AP+HRH QSALRF++EGKGAFTAVDGER Sbjct: 72 RVLVLENPALRGQSSITATLYAGLQLILPGEVAPSHRHNQSALRFIVEGKGAFTAVDGER 131 Query: 130 TAMSPGDFVITPSMTWHDHSNETNEPMFWLDGLDIPMVQFFDCSFAEGAKEDQQSISKPA 189 T M GDF++TP WHDH N +EP+ WLDGLD+P+V C FAE EDQQ +++ Sbjct: 132 TPMHTGDFILTPQWRWHDHGNPGSEPVVWLDGLDLPLVNLLGCGFAEDYPEDQQPVTRKE 191 Query: 190 GASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREAL-EKAKVG--DEWDACHGLKLKFS 246 G RY N+LP+ +R +SPIFNY Y+ +REAL + ++G DEW+ G KL++ Sbjct: 192 GDYLPRYAANMLPLRHQR-GNSSPIFNYRYDRSREALHDLTRMGDPDEWE---GYKLRYV 247 Query: 247 NPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQTFEWGPRDLF 306 NP TG + MP++G F+QLLPKGF + R+TD+T++ +EG G+ +G++TF + +D+F Sbjct: 248 NPVTGGYPMPSMGAFLQLLPKGFASRVARSTDSTIYHVVEGAGQVTIGNETFHFSAKDIF 307 Query: 307 VVPSWHWVTHEADADSVLFSFSDRPVQQKLDLFREDR 343 V P+WH V+ + D+VLFSFSD+PVQ+ L LFRE R Sbjct: 308 VAPTWHEVSFRSSEDTVLFSFSDKPVQEALGLFREAR 344 >PA2470 [Q] COG3435 Gentisate 1,2-dioxygenase Pae Pseudomonas aeruginosa Length = 353 Score = 378 bits (971), Expect = e-105, Method: Compositional matrix adjust. Identities = 184/348 (52%), Positives = 244/348 (70%), Gaps = 5/348 (1%) Query: 1 MEAVQKTPEREAFYKKIDGQNLSALWNVMNDLITPEPKSACRPHLWKFDQIRDYMNEAGK 60 M T +R FY++I Q+L+ LW ++ L+ +P C+ LW++ ++R ++ EAG Sbjct: 1 MHNDNSTEKRADFYRRIRQQHLTPLWEALHSLVPAQPAGGCQAALWRYRELRPFLLEAGD 60 Query: 61 LITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDIAPAHRHAQSALRFVLEGKG 120 LI+A+EA RRVLVLENP L GQS IT SL+AG+Q+++PG+IAP+HRH QSALRFV+EG G Sbjct: 61 LISAEEAVRRVLVLENPDLPGQSSITPSLYAGLQLILPGEIAPSHRHTQSALRFVVEGYG 120 Query: 121 AFTAVDGERTAMSPGDFVITPSMTWHDHSN----ETNEPMFWLDGLDIPMVQFFDCSFAE 176 A+T+VDGERT M PGDF+ITPS TWHDH N E EP+ WLDGLDIPM++FF +FAE Sbjct: 121 AYTSVDGERTRMEPGDFIITPSWTWHDHGNRAAAEGGEPVVWLDGLDIPMLRFFGATFAE 180 Query: 177 GAKEDQQSISKPAGASFARYGHNLLPIDQKRTSKTSPIFNYPYEYTREALEKAKVGDEWD 236 E Q + + G S ARYG N+LP+ + T+ TSP+F+YPY +REALE+ + D Sbjct: 181 NHDEPVQPLRRGEGDSLARYGSNMLPLRHQPTTPTSPLFSYPYARSREALERLARLERPD 240 Query: 237 ACHGLKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRATDATVFAAIEGKGRTRVGDQ 296 G KL++ NP TG +AMPTI T +QLLP GF R+TDA+V++ +EG G ++G + Sbjct: 241 PWEGHKLRYVNPATGGWAMPTIATCLQLLPAGFLGQPARSTDASVYSVVEGSGVAQIGGR 300 Query: 297 TFEWGPRDLFVVPSWHWVTHEADA-DSVLFSFSDRPVQQKLDLFREDR 343 F + +DLFVVPSW + EA A D VLFSFSDRPVQQ L + RE R Sbjct: 301 RFAFEAKDLFVVPSWAELRLEAGATDCVLFSFSDRPVQQALGILRESR 348