BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0127.0023.p (220 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll9608 [U] COG3451 Type IV secretory pathway, VirB4 components ... 360 1e-99 mlr6400 [U] COG3451 Type IV secretory pathway, VirB4 components ... 259 4e-69 RSc2581 [U] COG3451 Type IV secretory pathway, VirB4 components ... 249 3e-66 AGpT83 [U] COG3451 Type IV secretory pathway, VirB4 components A... 188 8e-48 mlr9251 [U] COG3451 Type IV secretory pathway, VirB4 components ... 101 1e-21 >mll9608 [U] COG3451 Type IV secretory pathway, VirB4 components Mlo Mesorhizobium loti Length = 816 Score = 360 bits (924), Expect = 1e-99, Method: Compositional matrix adjust. Identities = 173/214 (80%), Positives = 189/214 (88%) Query: 1 LLDAEFERLGEASVQAFETEGLIGTSAAPAVLAYLFHRIGDRLDGRPTLLIVDEGWLALD 60 LLDAE E LG+ASVQAFE EGL+G AAPAVL+YLFHR+ DRLDGRPTLLI+DEGWLALD Sbjct: 593 LLDAEAEHLGQASVQAFEIEGLVGAQAAPAVLSYLFHRVSDRLDGRPTLLIIDEGWLALD 652 Query: 61 DEDFAGQLREWLKTLRKKNASVIFATQSLSDVDGSAIAPAIIESCPTRLLLPNERAIEPQ 120 DE FAGQLREWLKTLRKKNASV+FATQSL+D+D SAIAPAIIESCPTRLLLPN RAIEPQ Sbjct: 653 DEGFAGQLREWLKTLRKKNASVVFATQSLADIDNSAIAPAIIESCPTRLLLPNARAIEPQ 712 Query: 121 ITAIYRRFGLNDRQIELLSRATPKRDYYCQSRRGNRMFELGLGEVALAFTAASSKTDQAA 180 I IYRRFGLNDRQIE+L+RATPKRDYYCQSRRGNR+FELGL EV LA AASSK+DQA Sbjct: 713 IADIYRRFGLNDRQIEILARATPKRDYYCQSRRGNRLFELGLSEVGLALCAASSKSDQAL 772 Query: 181 IAQLFAEHGPDGFVPAWLQHRGVAWAIDLIPNLV 214 I+ + +EHG DGF+PAWL R V WA DLIPNL Sbjct: 773 ISAIISEHGRDGFLPAWLHARNVGWAADLIPNLT 806 >mlr6400 [U] COG3451 Type IV secretory pathway, VirB4 components Mlo Mesorhizobium loti Length = 816 Score = 259 bits (661), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 129/211 (61%), Positives = 160/211 (75%), Gaps = 1/211 (0%) Query: 1 LLDAEFERLGEASVQAFETEGLIGT-SAAPAVLAYLFHRIGDRLDGRPTLLIVDEGWLAL 59 LLDA+ +RL + VQ FETE L+ + SA VL YLFHR+ +R DGRPTLL++DE W+ L Sbjct: 594 LLDADDDRLALSDVQCFETEELMHSQSAVLPVLTYLFHRLEERFDGRPTLLMLDEAWVYL 653 Query: 60 DDEDFAGQLREWLKTLRKKNASVIFATQSLSDVDGSAIAPAIIESCPTRLLLPNERAIEP 119 D+ FA ++REWLK LRKKN SVIFATQSL+D+ GSAIAPAIIESCP R+ LPN+RA+EP Sbjct: 654 DNPLFAARIREWLKVLRKKNVSVIFATQSLADIAGSAIAPAIIESCPQRIFLPNDRAVEP 713 Query: 120 QITAIYRRFGLNDRQIELLSRATPKRDYYCQSRRGNRMFELGLGEVALAFTAASSKTDQA 179 Q Y RFGLNDRQIEL++RATPKR YY QSR GNR+FELGLG +ALA AS Q+ Sbjct: 714 QARTAYERFGLNDRQIELIARATPKRHYYLQSRCGNRLFELGLGPIALALCGASGPAAQS 773 Query: 180 AIAQLFAEHGPDGFVPAWLQHRGVAWAIDLI 210 I ++ ++HG D FV +L RG+ WA +L+ Sbjct: 774 LIDRVLSKHGQDSFVFRFLGARGLDWAAELL 804 >RSc2581 [U] COG3451 Type IV secretory pathway, VirB4 components Rso Ralstonia solanacearum Length = 816 Score = 249 bits (636), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 124/213 (58%), Positives = 155/213 (72%), Gaps = 1/213 (0%) Query: 1 LLDAEFERLGEASVQAFETEGLIGTSAAP-AVLAYLFHRIGDRLDGRPTLLIVDEGWLAL 59 LLDA+ +RLG A VQ FE E L+ + AA +VL YLF R +R DG PTLLI+DE WL L Sbjct: 587 LLDADRDRLGSADVQCFEMEELMASPAAVRSVLGYLFARFDERFDGSPTLLILDESWLFL 646 Query: 60 DDEDFAGQLREWLKTLRKKNASVIFATQSLSDVDGSAIAPAIIESCPTRLLLPNERAIEP 119 DD FA ++R+WLKTLRK+N SV+FATQSL+D+ S+IA AIIESC R+ LPN +A EP Sbjct: 647 DDPVFAARIRQWLKTLRKRNVSVVFATQSLADIQNSSIAAAIIESCAVRIFLPNPQATEP 706 Query: 120 QITAIYRRFGLNDRQIELLSRATPKRDYYCQSRRGNRMFELGLGEVALAFTAASSKTDQA 179 QI IY FGLN RQIE+++ A PK+DYY QSR GNR+F+L LG V LAF AS+ DQ Sbjct: 707 QIRTIYEGFGLNSRQIEIVATAQPKQDYYYQSRLGNRLFDLDLGPVVLAFAGASTPQDQR 766 Query: 180 AIAQLFAEHGPDGFVPAWLQHRGVAWAIDLIPN 212 I ++ + G GF AWL+HRG+ WA DL+P+ Sbjct: 767 DIERVLTQAGAPGFAGAWLRHRGLDWAADLLPS 799 >AGpT83 [U] COG3451 Type IV secretory pathway, VirB4 components Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 822 Score = 188 bits (477), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 100/213 (46%), Positives = 131/213 (61%), Gaps = 3/213 (1%) Query: 1 LLDAEFERLGEASVQAFETEGLI--GTSAAPAVLAYLFHRIGDRLDGRPTLLIVDEGWLA 58 LLDAE + L + Q FE E L+ G VL Y+F R+ RL G P+L+I+DE WL Sbjct: 609 LLDAEEDGLALGAFQCFEVEELMNMGERNLVPVLLYMFRRVEKRLTGAPSLIIIDEAWLM 668 Query: 59 LDDEDFAGQLREWLKTLRKKNASVIFATQSLSDVDGSAIAPAIIESCPTRLLLPNERAIE 118 L F ++REWLK LRK N +V+ ATQS+SD + S I + ESCPT++ LPN A E Sbjct: 669 LGHPTFRDKIREWLKVLRKANCAVVLATQSISDAERSGIIDVLKESCPTKICLPNGAARE 728 Query: 119 PQITAIYRRFGLNDRQIELLSRATPKRDYYCQSRRGNRMFELGLGEVALAFTAASSKTDQ 178 P Y R G N+RQIE+++ A PKR+YY S G R+F++ LG VAL+F AS K D Sbjct: 729 PGTREFYERIGFNERQIEIVATAMPKREYYVASPEGRRLFDMSLGPVALSFVGASGKEDL 788 Query: 179 AAIAQLFAEHGPDGFVPAWLQHRGVAWAIDLIP 211 +I L +E+G D + WLQ RG+A A L P Sbjct: 789 KSIRALHSENGADWPI-HWLQQRGIAHAETLFP 820 >mlr9251 [U] COG3451 Type IV secretory pathway, VirB4 components Mlo Mesorhizobium loti Length = 803 Score = 101 bits (251), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 64/192 (33%), Positives = 108/192 (56%), Gaps = 9/192 (4%) Query: 12 ASVQAFE-TEGLIGTSAAPAVLAYLFHRIGDRLDGRPTLLIVDEGWLALDDEDFAGQLRE 70 +S+ F+ T L + + A L Y+FHRI + LDG+P ++ +DEGW LD++ F+ +++ Sbjct: 611 SSIFGFDMTSVLSDATTSTAALLYIFHRIEELLDGQPVMIFLDEGWKLLDNDIFSYFIKD 670 Query: 71 WLKTLRKKNASVIFATQSLSDVDGSAIAPAIIESCPTRLLLPNERAIEPQITAIYRRFGL 130 LKT+RK N + F TQS +D+ S+IA +IE PT + PN +A E +R FGL Sbjct: 671 KLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEES----HRAFGL 726 Query: 131 NDRQIELLSRATPK-RDYYCQSRRGNRMFELGLGEVALAFTAASSKTDQAA-IAQLFAEH 188 ++R+I+ + P+ R + + + + + L LG + A S + + + L A Sbjct: 727 SEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELDALRARV 786 Query: 189 G--PDGFVPAWL 198 G PD ++P +L Sbjct: 787 GDDPDVWLPVFL 798