BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0124.0007.p (532 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA2102 [L] COG3344 Retron-type reverse transcriptase Mac Methano... 132 2e-30 SA2010 [L] COG3344 Retron-type reverse transcriptase Sau Staphyl... 118 4e-26 FN0161 [L] COG3344 Retron-type reverse transcriptase Fnu Fusobac... 117 5e-26 STM3846 [L] COG3344 Retron-type reverse transcriptase Sty Salmon... 96 2e-19 all2961 [L] COG3344 Retron-type reverse transcriptase Nos Nostoc... 86 2e-16 >MA2102 [L] COG3344 Retron-type reverse transcriptase Mac Methanosarcina acetivorans str.C2A Length = 563 Score = 132 bits (332), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 81/275 (29%), Positives = 142/275 (51%), Gaps = 20/275 (7%) Query: 29 FLALSTAELKKIWWF---RGRMYQKFEISKGGQKKRVISAPDRRLKMLQTKIASSLASIY 85 F L+ + LK++ F + + Y F++ K R I+AP +++K +Q I ++ Sbjct: 59 FCKLTNSSLKQVNLFLSNKKKGYITFKLPKKNGDFREINAPSKKMKYIQRWILDNILYKL 118 Query: 86 RPRNPVHGFVDGRSVKTNATAHLRSKFVLNLDIEGFFMAITEGRVSGLMGALGVDGRVAE 145 + HGF+ G+++ TNA H+ VL +DI+ FF +I V + + G ++A Sbjct: 119 NSGDYAHGFIPGKTIFTNAKVHVNQDLVLGVDIKDFFPSINFRSVYYVFKSAGYTKKIAW 178 Query: 146 ILARICCNEGVLPQGAPSSPVISNMICFRMDKELQTIAKESRCIYTRYADDITFSSYQPL 205 LA +C LPQGAP+SP+++N++ ++DK++ Y+RYADD+T S Sbjct: 179 TLADLCTYHWKLPQGAPTSPMLANLVALKLDKKIAKYCARRNFRYSRYADDVTIS----- 233 Query: 206 TLLFEGPPPPAGNFPPDLLKDRLRGAFRSNGFAINPQKIHYADKHSRRTVTGLKINEFVN 265 G++ + K+++ G +GF +N +K K SR+ VTGL +N+ V+ Sbjct: 234 -----------GSYKLPMHKEKIIGIIEDDGFVVNHEKTRMFSKGSRQKVTGLVVNDKVS 282 Query: 266 VDRKYVRNTRAALFVTETRGAAVAQKILKDKYGRE 300 + R +N +A + +T G VA+ D + RE Sbjct: 283 IGRNKKKNLKAIVNNIQTNG-PVAENRSNDPFFRE 316 >SA2010 [L] COG3344 Retron-type reverse transcriptase Sau Staphylococcus aureus N315 Length = 338 Score = 118 bits (295), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 84/300 (28%), Positives = 142/300 (47%), Gaps = 29/300 (9%) Query: 25 ELLKFLALSTAELKKIWWFRGRMYQKFEISKGGQKK-RVISAPDRRLKMLQTKIASSLAS 83 EL + + + K + Y +I K + + R + P+ LK +Q I ++ Sbjct: 21 ELRNMIGIKSDYFYKCLYVNDHFYNVIKIPKRKKDEYRELMIPNMALKNIQRWILDNV-- 78 Query: 84 IYRPRNPVH----GFVDGRSVKTNATAHLRSKFVLNLDIEGFFMAITEGRVSGLMGALGV 139 +YR + VH GFV +S+ NA H+ K++L +DIE FF +IT +V + +G Sbjct: 79 LYRRQ--VHKCATGFVPRKSIVNNAIPHVGQKYILKMDIENFFPSITFKQVRKIFSEMGY 136 Query: 140 DGRVAEILARICCNEGVLPQGAPSSPVISNMICFRMDKELQTIAKESRCIYTRYADDITF 199 +A LA +C LPQGAP+SP I+N+I + +DK + + +++ YTRYADDIT Sbjct: 137 KFELATALANLCTVNNQLPQGAPTSPYIANIIFYNIDKRIFSYCQKNNLRYTRYADDITI 196 Query: 200 SSYQPLTLLFEGPPPPAGNFPPDLLKDRLRGAFRSNGFAINPQKIHYADKHSRRTVTGLK 259 S G+ K+ +R F IN K R+ VTG+ Sbjct: 197 S----------------GSNKVSFSKEIIREIVNQYNFRINESKTIMFKPGDRKKVTGII 240 Query: 260 INEFVNVDRKYVRNTRAALFVTETRGAAVAQKILKDKYG--REATLASHLRGRISWVGHI 317 +NE ++V + +R R ++ G + + ++++ Y E + G+IS++ I Sbjct: 241 VNEKISVPKTLIREVRKQIYFVNKFG--LEEHLIRNNYSLDYEQQFIMSIYGKISFIKMI 298 >FN0161 [L] COG3344 Retron-type reverse transcriptase Fnu Fusobacterium nucleatum Length = 349 Score = 117 bits (294), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 83/300 (27%), Positives = 133/300 (44%), Gaps = 25/300 (8%) Query: 45 GRMYQKFEISKGGQKKRVISAPDRRLKMLQTKIASS-LASIYRPR--NPVHGFVDGRSVK 101 + Y++FEI K R IS P LK +Q I + L I + + + S+K Sbjct: 67 SKFYREFEIKKKNGGSRKISEPLPSLKEIQKWILENILNKIQKEKVSKYAKAYQKKISIK 126 Query: 102 TNATAHLRSKFVLNLDIEGFFMAITEGRVSGLMGALGVDGRVAEILARICCNEGVLPQGA 161 N H K VL+LDI FF+ I ++ + LG ++ + + +C LPQGA Sbjct: 127 ENVKFHRGQKKVLSLDITNFFLNIKIDKIYEVFYNLGYSKSLSTLFSNLCTLNYSLPQGA 186 Query: 162 PSSPVISNMICFRMDKELQTIAKESRCIYTRYADDITFSSYQPLTLLFEGPPPPAGNFPP 221 P+SP++SN++ D E++ I E R YTRYADD+TFS G+F Sbjct: 187 PTSPILSNIVMLNFDNEIEKIVLEKRIRYTRYADDMTFS----------------GDFLE 230 Query: 222 DLLKDRLRGAFRSNGFAINPQKIHYADKHSRRTVTGLKINEFVNVDRKYVRNTRAALFVT 281 + ++ G IN +K ++ VTG+ +NE + + RK R ++ Sbjct: 231 KEIIKYVKENLNKIGLKINNKKTRVRKNWQQQMVTGIIVNEKIQISRKKRDELRQTMYYI 290 Query: 282 ETRG--AAVAQKILKDKYGREATLASHLRGRISWVGHIKGPSDPIFRGLASRYNKLFPSE 339 E G + + K +K+K SHL+G + + I +F + N F + Sbjct: 291 EKYGIDSFLKYKGIKNK----VYYLSHLKGILEYAYFINKNDKKLFNYIEYLKNNFFKEK 346 >STM3846 [L] COG3344 Retron-type reverse transcriptase Sty Salmonella typhimurium LT2 Length = 289 Score = 95.5 bits (236), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 77/230 (33%), Positives = 118/230 (51%), Gaps = 36/230 (15%) Query: 48 YQKFEISK-GGQKKRVISAPDRRLKMLQTKIASSLASIYRPRNPVH----GFVDGRSVKT 102 Y+ ++I+K KKR I+ P + LK +Q +I L + PVH + G S+KT Sbjct: 7 YKIYKIAKRNSDKKRTIAHPSKELKFIQREITEYLTD----KLPVHECAFAYKKGSSIKT 62 Query: 103 NATAHLRSKFVLNLDIEGFFMAIT------EGRVSGLMGALGVDGRVAEILARICC---- 152 NA HL +K++L +D E FF +IT + R++ + L D +V +L I Sbjct: 63 NAQVHLHTKYLLKMDFENFFPSITPRLFFSKLRLANI--DLTADDKV--LLENILFFKSK 118 Query: 153 -NEGV-LPQGAPSSPVISNMICFRMDKELQTIAKESRCIYTRYADDITFSSYQPLTLLFE 210 N + L GAPSSP+ISN + + D E+Q I + YTRYADD+TFS+ +LF+ Sbjct: 119 RNSNLRLSIGAPSSPLISNFVMYFWDIEVQEICSKIGVNYTRYADDLTFSTNNK-DVLFD 177 Query: 211 GPPPPAGNFPPDLLKDRLRGAFRSNGFAINPQKIHYADKHSRRTVTGLKI 260 PD+L++ L + IN +K ++ K R VTG+ + Sbjct: 178 ---------IPDMLENVL-PKYSLGRIRINHEKTVFSSKGHNRHVTGITL 217 >all2961 [L] COG3344 Retron-type reverse transcriptase Nos Nostoc sp. PCC 7120 Length = 306 Score = 86.3 bits (212), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 109/240 (45%), Gaps = 18/240 (7%) Query: 20 LTCEAELLKFLALSTAELKKIWWFRGRMYQKFEISKGGQKKRVISAPDRRLKMLQTKIAS 79 L +A+L L +S + L ++ +K I +K R++ P LK++Q +I Sbjct: 21 LQSKAKLATLLGVSRSRLIQLARSESLYIEKEIIHPRRKKSRLVEKPKYELKLIQKRIEG 80 Query: 80 SLASIYRPRNPVHGFVDGRSVKTNATAHLRSKFVLNLDIEGFFMAITEGRVSGLMGA-LG 138 L I P+ +H GRS +NA AHL S + +LDI +F + RV + Sbjct: 81 LLKRIEIPKY-IHAPTKGRSYVSNAKAHLNSYVISSLDIVKYFPSTKSKRVYWFFHTRMK 139 Query: 139 VDGRVAEILARICCNEGVLPQGAPSSPVISNMICFRMDKELQTIAKESRCIYTRYADDIT 198 VA IL ++ + LP G+P SP++S M +E+ I +++ CI T Y DD+T Sbjct: 140 CSPDVAAILTKLSTYKEHLPTGSPLSPILSYFAHIDMWEEINNIVEDAGCILTVYMDDVT 199 Query: 199 FSSYQPLTLLFEGPPPPAGNFPPDLLKDRLRGAFRSNGFAINPQKIHYADKHSRRTVTGL 258 S G++ L +++ F G + N +K Y + R +TG+ Sbjct: 200 IS----------------GDYVSRNLIWQIKKKFHRYGLSSNTKKEKYYIGKNSRKITGV 243