BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0121.0010.p (225 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00383 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium... 311 1e-84 mlr1410 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium ... 244 9e-65 RSc2594 [O] COG0625 Glutathione S-transferase Rso Ralstonia sola... 239 3e-63 RSc2926 [O] COG0625 Glutathione S-transferase Rso Ralstonia sola... 88 2e-17 NMB1953 [O] COG0625 Glutathione S-transferase Nme Neisseria meni... 81 2e-15 >SMc00383 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 228 Score = 311 bits (796), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 151/221 (68%), Positives = 170/221 (76%), Gaps = 1/221 (0%) Query: 2 AALKLISHKLCPYVQRAVIALREKGVPFERIDIDLANKPDWFLKISPLGKVPVLVVTRDD 61 A L LISH LCPYVQRA IAL EKGVPFER+DIDLANKPDWFLKISPLGKVP+L +++D Sbjct: 3 AQLTLISHHLCPYVQRAAIALHEKGVPFERVDIDLANKPDWFLKISPLGKVPLLRISQDG 62 Query: 62 GKEVALFESNVICEYIEETQAGAKLHLQDPLVRAEHRAWMEFGSAILGDLWGLETATDAA 121 G E LFES VICEY+EETQAG KLH DPL RA HR WMEFGSAIL DLW ET D A Sbjct: 63 G-EAILFESTVICEYLEETQAGPKLHPADPLARARHRGWMEFGSAILSDLWVYETTQDGA 121 Query: 122 AFESKRQAVAAKFARVEVALGAGPFFAGQNFSLVDAVFAPIFRYFDLFDQLTDLAVFTHT 181 E+KR + +KFA VE LG+GP+FAG FSLVDAVFAPIFRYFD+FD ++D VF Sbjct: 122 TLEAKRNVLKSKFATVEAELGSGPYFAGSAFSLVDAVFAPIFRYFDVFDTISDSGVFQGL 181 Query: 182 PKLRAWRSALAQRPSVRSAVSPDYPALLHAFLVGHRAHMLK 222 P++ AWR ALA RPSV++AV DYP L AFL H A +LK Sbjct: 182 PRVTAWREALAGRPSVKAAVGEDYPQRLMAFLEKHEAALLK 222 >mlr1410 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium loti Length = 249 Score = 244 bits (624), Expect = 9e-65, Method: Compositional matrix adjust. Identities = 124/215 (57%), Positives = 153/215 (71%), Gaps = 7/215 (3%) Query: 4 LKLISHKLCPYVQRAVIALREKGVPFERIDIDLANKPDWFLKISPLGKVPVLVVTRDDGK 63 L L+SH LCPYVQRA I+L EKGVPFERIDIDLA+KPDWF ISPLGKVP+L V R+ G Sbjct: 11 LILVSHPLCPYVQRAAISLTEKGVPFERIDIDLADKPDWFKAISPLGKVPLLRVQRN-GD 69 Query: 64 EVALFESNVICEYIEETQAGAKLHLQDPLVRAEHRAWMEFGSAILGDLWGLETATDAAAF 123 E +FES VI E++EETQA LH DP RA HRAW+E+GSAIL + L +A A F Sbjct: 70 ETTIFESAVILEFLEETQAN-PLHPADPYARARHRAWIEYGSAILNAIGRLYSAPTEAGF 128 Query: 124 ESKRQAVAAKFARVEV-----ALGAGPFFAGQNFSLVDAVFAPIFRYFDLFDQLTDLAVF 178 ++ +A+ F R+E AL +GP+FAG FSLVDAV P+FRY D FD++ D ++ Sbjct: 129 LAESRALTGMFDRLEAELADDALRSGPWFAGDRFSLVDAVHGPVFRYLDAFDRIGDFSIL 188 Query: 179 THTPKLRAWRSALAQRPSVRSAVSPDYPALLHAFL 213 P+++AWR AL RPS+R AV+PDYP LHAFL Sbjct: 189 DGKPRVQAWRQALNDRPSIRHAVAPDYPQRLHAFL 223 >RSc2594 [O] COG0625 Glutathione S-transferase Rso Ralstonia solanacearum Length = 224 Score = 239 bits (610), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 116/217 (53%), Positives = 148/217 (68%) Query: 2 AALKLISHKLCPYVQRAVIALREKGVPFERIDIDLANKPDWFLKISPLGKVPVLVVTRDD 61 + L LISH LCP+VQRA I L EKGVPFERI++DLA KPDWFL +SP GKVP+L V R D Sbjct: 4 STLTLISHPLCPFVQRAAIVLLEKGVPFERINVDLAAKPDWFLALSPTGKVPLLKVVRAD 63 Query: 62 GKEVALFESNVICEYIEETQAGAKLHLQDPLVRAEHRAWMEFGSAILGDLWGLETATDAA 121 ++ LFES VICEY+EETQAG KL+ +D L RA+ RAW+EFG+A L D W A+D Sbjct: 64 REDAVLFESMVICEYLEETQAGGKLYAEDALSRAQQRAWIEFGTATLADAWQFLNASDRT 123 Query: 122 AFESKRQAVAAKFARVEVALGAGPFFAGQNFSLVDAVFAPIFRYFDLFDQLTDLAVFTHT 181 + KR A K ++E A+ A P+F+G FS+VDAV+AP+FRYFD+ D +F Sbjct: 124 TSDDKRAAFRKKLQQLEKAVAAEPYFSGSTFSMVDAVYAPLFRYFDILDPKVSQPIFEGL 183 Query: 182 PKLRAWRSALAQRPSVRSAVSPDYPALLHAFLVGHRA 218 P+++AWR+AL R SV +AV DY L H+A Sbjct: 184 PRVKAWRAALGARESVIAAVGEDYAERFGQHLRLHQA 220 >RSc2926 [O] COG0625 Glutathione S-transferase Rso Ralstonia solanacearum Length = 203 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 60/202 (29%), Positives = 100/202 (49%), Gaps = 13/202 (6%) Query: 6 LISHKLCPYVQRAVIALREKGVPFERIDIDLANKPDWFLKISPLGKVPVLVVTRDDGKEV 65 L S CP+ QR + L EKG+ FE D+DL NKP+ ++P G+VP+LV +++ Sbjct: 4 LYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDIAVMNPYGQVPILV-----ERDL 58 Query: 66 ALFESNVICEYIEETQAGAKLHLQDPLVRAEHRAWM-EFGSAILGDLWGLETATDAAAFE 124 L+ESN+I EYI+E +L DP+ RA R ++ F + + LE AA + Sbjct: 59 ILYESNIINEYIDERFPHPQLMPADPVQRARARLFLFNFEKELFTHVSVLENEKGKAAEK 118 Query: 125 SKRQAVAA---KFARVEVALGAGPFFAGQNFSLVDAVFAPIFRYFDLFDQLTDLAVFTHT 181 S +A AA + ++ + G+ FS++D AP+ D + + + + Sbjct: 119 SHEKARAAIRDRLTQLAPIFLKNKYMLGEEFSMLDVAIAPLLWRLDHY----GIELSKNA 174 Query: 182 PKLRAWRSALAQRPSVRSAVSP 203 L + + RP+ A++P Sbjct: 175 APLMKYAERIFSRPAYIEALTP 196 >NMB1953 [O] COG0625 Glutathione S-transferase Nme Neisseria meningitidis MC58 Length = 201 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 49/169 (28%), Positives = 82/169 (48%), Gaps = 7/169 (4%) Query: 4 LKLISHKLCPYVQRAVIALREKGVPFERIDIDLANKPDWFLKISPLGKVPVLVVTRDDGK 63 + L S CP+ R L EKG+ FE D+D+ NKP+ ++P +VPVLV + Sbjct: 2 MTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVMNPYNQVPVLV-----ER 56 Query: 64 EVALFESNVICEYIEETQAGAKLHLQDPLVRAEHRAWM-EFGSAILGDLWGLETATDAAA 122 ++ L ESN+I EYI+E +L DP++R R + + + LE Sbjct: 57 DLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELFNHVQVLENPAATNK 116 Query: 123 FESK-RQAVAAKFARVEVALGAGPFFAGQNFSLVDAVFAPIFRYFDLFD 170 ++K R+A+ + + + G++FS++D AP+ D +D Sbjct: 117 EQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWRLDHYD 165